FastQCFastQC Report
Sun 19 Apr 2015
000000000-AE2TW l01n02.33200000008a68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE2TW l01n02.33200000008a68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16299268
Sequences flagged as poor quality0
Sequence length12
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGAACCTCCA14327618.790339541628494No Hit
AGCAGTCGCGAT10955576.721510438382877No Hit
AATCAGTCTCGT9972696.1184894929023805No Hit
TTCAGACTACCA9561835.866416823135861No Hit
GAGTGAGTACAA8837745.422169879039966No Hit
ATTTCGACATGC8602975.278132735776846No Hit
TGCCCATTTAAG8387415.145881397864002No Hit
GCGGATGTGACT7916744.857113828670098No Hit
CAGGGTGATACC7852884.817934155079848No Hit
ATCGAATGCGGC7676674.70982500563829No Hit
AATCCGTACAGC7668894.705051785147652No Hit
AACGGATAAACG7242314.443334510482312No Hit
AGTTCAGACGCT7000954.295254240865296No Hit
ACCGTGCATAGG6561964.025923127345351No Hit
CTTATCAAGGCG6092793.738075844878433No Hit
TAATGACCACGC5656423.470352165508291No Hit
TGCCTACAAGCA5513533.382685651895533No Hit
TGAAAACCTCCA279570.17152304017579192No Hit
TGAGAACCTCAA207220.12713454371079733No Hit
AGCAGTCGCGCT183300.11245903803778182No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAG2250.06.00000055
GAAATCG550.0091607636.00000052
GGGTGCT2750.06.00000053
GACAGAG550.0091607636.00000051
GTCGCAT550.0091607636.00000055
GTCGCAC550.0091607636.00000055
GTCGAGC550.0091607636.00000055
AGCCCTC550.0091607636.00000051
TAGAATG2250.06.00000052
CATGACC1101.8153878E-76.00000052
TAGAAGC550.0091607636.00000055
GATCCTA550.0091607636.00000054
GGCAAAG1101.8153878E-76.00000055
CCTACGA550.0091607636.00000053
CGATTGC3450.06.00000053
GACCCCA550.0091607636.00000055
GACTTGC2750.06.00000056
AACGGAC4400.06.00000051
GGAAGAT550.0091607636.00000053
GCACAGC10100.06.00000056
Produced by FastQC (version 0.11.2)