##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AE2TW l01n01.33100000008a6b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16299268 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.6916893445767 32.0 32.0 33.0 30.0 34.0 2 26.774282194758683 33.0 32.0 33.0 2.0 34.0 3 31.506325314731924 33.0 31.0 33.0 29.0 34.0 4 31.620034470259647 33.0 32.0 33.0 29.0 34.0 5 32.081565380727525 33.0 32.0 33.0 31.0 34.0 6 33.51189403106937 33.0 32.0 37.0 32.0 37.0 7 34.948663829565845 37.0 33.0 37.0 32.0 37.0 8 35.883430470619906 37.0 37.0 37.0 33.0 37.0 9 35.53581780482412 37.0 37.0 37.0 33.0 37.0 10-11 33.925433215773865 35.0 33.0 35.5 32.0 37.0 12-13 35.082147492758565 35.5 34.0 36.5 34.0 37.5 14-15 36.43763836510941 38.0 37.0 38.0 34.0 38.0 16-17 36.523593268114865 38.0 37.0 38.0 34.0 38.0 18-19 36.42479610127277 38.0 37.0 38.0 33.5 38.0 20-21 36.77477194067856 38.0 37.5 38.0 35.5 38.0 22-23 36.64077650603696 38.5 37.0 38.5 33.5 38.5 24-25 37.09393219376477 39.0 37.0 39.0 34.0 39.0 26-27 37.458538107355494 39.0 37.5 39.0 35.5 39.0 28-29 37.091219311198515 38.0 37.5 39.0 34.5 39.0 30-31 36.94620411787818 38.5 37.5 39.0 34.0 39.0 32-33 36.84751839775872 39.0 37.5 39.0 33.5 39.0 34-35 37.049646339946065 38.0 37.5 39.0 34.5 39.0 36-37 36.61078976061992 38.5 37.0 39.0 33.0 39.0 38-39 37.29721555593785 39.0 37.5 39.0 35.0 39.0 40-41 36.95500356212315 39.0 37.5 39.0 33.5 39.0 42-43 36.701534909420474 39.0 37.0 39.0 33.0 39.0 44-45 36.417336471797384 39.0 37.0 39.0 32.5 39.0 46-47 36.25287193878891 38.0 37.0 39.0 32.5 39.0 48-49 36.27654741918471 38.0 37.0 39.0 32.5 39.0 50-51 36.331847049818435 38.0 37.0 39.0 32.0 39.0 52-53 36.434916647790565 38.5 37.0 39.0 32.5 39.0 54-55 35.97784566153523 38.0 36.5 39.0 32.0 39.0 56-57 36.10440876854102 38.0 37.0 39.0 32.0 39.0 58-59 35.40822376195054 38.0 36.0 39.0 23.0 39.0 60-61 36.4056366212274 38.5 37.0 39.0 32.5 39.0 62-63 35.915026245350404 38.5 37.0 39.0 30.5 39.0 64-65 35.64183587876462 38.0 36.5 39.0 25.0 39.0 66-67 35.758029164254495 38.0 36.5 39.0 30.0 39.0 68-69 36.060161904203305 38.0 37.0 39.0 31.5 39.0 70-71 35.55742125965412 38.0 36.5 39.0 25.0 39.0 72-73 35.42139947634458 38.0 36.0 39.0 23.5 39.0 74-75 35.953750162277224 39.0 37.0 39.0 26.0 39.0 76-77 35.681942005002924 38.0 36.5 39.0 24.5 39.0 78-79 35.922811901736935 38.5 36.5 39.0 26.0 39.0 80-81 36.3802467386879 39.0 37.0 39.0 32.0 39.0 82-83 35.50085328985326 38.0 36.5 39.0 24.5 39.0 84-85 35.515894272061786 38.0 36.0 38.5 25.0 39.0 86-87 36.34269753709185 39.0 37.0 39.0 32.0 39.0 88-89 35.61287463952369 38.5 37.0 39.0 24.5 39.0 90-91 35.547497991934364 38.0 37.0 39.0 23.5 39.0 92-93 35.7057536571581 38.0 37.0 39.0 29.5 39.0 94-95 35.34964024151269 38.5 36.5 39.0 23.5 39.0 96-97 35.679920564530875 38.5 37.0 39.0 29.0 39.0 98-99 35.94181717240308 38.0 36.5 39.0 30.5 39.0 100-101 35.56928522802374 38.5 36.0 39.0 25.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.22034727506412466 1101 2 7.7619410703251575 1101 3 1.1113585762115505 1101 4 1.150926251319465 1101 5 0.6756841571824559 1101 6 1.045544207711771 1101 7 1.288970877481674 1101 8 0.24003860983935255 1101 9 0.37918415328168464 1101 10-11 0.5168097048299316 1101 12-13 0.4465963894664 1101 14-15 0.5098126287781071 1101 16-17 0.3695384073615031 1101 18-19 0.2293305615882275 1101 20-21 0.1643502531326888 1101 22-23 0.20320931784053897 1101 24-25 0.16898001249846573 1101 26-27 0.32435386339543726 1101 28-29 0.2898809632221955 1101 30-31 0.3750828222135496 1101 32-33 0.25391595259439725 1101 34-35 0.3082855389947241 1101 36-37 0.2749396826888102 1101 38-39 0.34587010638293947 1101 40-41 0.4865482157156933 1101 42-43 0.37844559249679577 1101 44-45 0.28707976072894326 1101 46-47 0.35719260388961516 1101 48-49 0.30529022643305836 1101 50-51 0.3942387945692474 1101 52-53 0.3497598269362854 1101 54-55 0.2824398793462777 1101 56-57 0.24531473391617453 1101 58-59 0.23156046266139185 1101 60-61 0.23789702918949018 1101 62-63 0.15970401437287052 1101 64-65 0.281039168569059 1101 66-67 0.3500833845979372 1101 68-69 0.4798778268700232 1101 70-71 0.17808029219080623 1101 72-73 0.3041501823985868 1101 74-75 0.4568856229635614 1101 76-77 0.36902037838502366 1101 78-79 0.35403966630329364 1101 80-81 0.4452600266897093 1101 82-83 0.48996848348561883 1101 84-85 0.5633630014282431 1101 86-87 0.41630645121795595 1101 88-89 0.28966328873380576 1101 90-91 0.3486578646141112 1101 92-93 0.39837574930620434 1101 94-95 0.32719733416374197 1101 96-97 0.3077700576228892 1101 98-99 0.3804103559156715 1101 100-101 0.30416323708112003 1102 1 0.22918752028401812 1102 2 7.915074911381275 1102 3 1.2709611852913056 1102 4 1.2009387208238884 1102 5 0.6848257263591613 1102 6 1.026551669350063 1102 7 1.192266651857878 1102 8 0.2130492060128759 1102 9 0.2994483924954707 1102 10-11 0.5293119612931392 1102 12-13 0.4341794571351585 1102 14-15 0.44497127889618326 1102 16-17 0.28832559625057286 1102 18-19 0.13827968448207884 1102 20-21 0.06541267006626583 1102 22-23 0.28003021506492587 1102 24-25 0.3552967379046805 1102 26-27 0.39115920729661724 1102 28-29 0.2664063388092046 1102 30-31 0.3354673655279399 1102 32-33 0.2780833360712336 1102 34-35 0.3095297475873764 1102 36-37 0.0492440761934958 1102 38-39 0.2648189920647539 1102 40-41 0.20258497003787568 1102 42-43 0.28306338518571295 1102 44-45 0.2824420350082022 1102 46-47 0.3664316970304071 1102 48-49 0.33269676025917505 1102 50-51 0.3413225300496592 1102 52-53 0.37570831976794494 1102 54-55 0.3648695770278749 1102 56-57 0.1940911758735453 1102 58-59 0.2509345780043333 1102 60-61 0.10956697491894118 1102 62-63 0.21744288808941548 1102 64-65 0.27553327385137294 1102 66-67 0.47856769983242486 1102 68-69 0.520391327358297 1102 70-71 0.29293638504076824 1102 72-73 0.2759799445282809 1102 74-75 0.5378419007041586 1102 76-77 0.4803393073081068 1102 78-79 0.36209855873337915 1102 80-81 0.4668875257175529 1102 82-83 0.5358242889019351 1102 84-85 0.5765195135693162 1102 86-87 0.518121645565472 1102 88-89 0.30118387341249786 1102 90-91 0.3651425438642022 1102 92-93 0.4723793038206594 1102 94-95 0.3716875154260819 1102 96-97 0.3690996943884173 1102 98-99 0.4805318284303013 1102 100-101 0.41708989146946607 1103 1 0.439507039085445 1103 2 7.832319815837678 1103 3 1.1280150747771955 1103 4 1.191019929232045 1103 5 0.6175860008275471 1103 6 1.0857936501078385 1103 7 1.2326574019177343 1103 8 0.23991580434599058 1103 9 0.35839913035220405 1103 10-11 0.5430811629186323 1103 12-13 0.4588230712142263 1103 14-15 0.43523961631384367 1103 16-17 0.3713287121160178 1103 18-19 0.34349963443611387 1103 20-21 0.1949957754306837 1103 22-23 0.1890534937791557 1103 24-25 0.2528419670844926 1103 26-27 0.3276193790439521 1103 28-29 -0.19705296678254314 1103 30-31 0.2372451116705676 1103 32-33 0.13976485455054188 1103 34-35 0.384842992463966 1103 36-37 -0.2823312002401721 1103 38-39 0.15891536384283 1103 40-41 -0.21995330841936322 1103 42-43 0.04024892712850914 1103 44-45 0.29492491402691456 1103 46-47 0.3648210150153375 1103 48-49 0.3338989992051751 1103 50-51 0.45823520880579593 1103 52-53 0.43171042363159273 1103 54-55 0.36321131663756745 1103 56-57 0.18652646897966463 1103 58-59 0.18200104560717278 1103 60-61 0.2175103049821132 1103 62-63 0.2092885791951531 1103 64-65 0.40506533853248783 1103 66-67 0.38152657928921485 1103 68-69 0.5704122219124557 1103 70-71 0.4238592304834583 1103 72-73 0.2845241454011358 1103 74-75 0.43362559298712 1103 76-77 0.576750657680698 1103 78-79 0.4344546894074952 1103 80-81 0.262230151860706 1103 82-83 -0.15429540283270882 1103 84-85 0.07342325989219489 1103 86-87 0.43347213559906805 1103 88-89 0.2524807138579632 1103 90-91 0.3019368820257 1103 92-93 0.19569261632395296 1103 94-95 0.13958735367415187 1103 96-97 0.19315863730198402 1103 98-99 0.5072272818231909 1103 100-101 0.4648664520503658 2112 1 0.17674302709763623 2112 2 7.791769789328534 2112 3 1.1852933638501 2112 4 0.9813964221724696 2112 5 0.5860849100514933 2112 6 1.0259478722471371 2112 7 1.3903889491087185 2112 8 -0.006915041411190259 2112 9 0.44481033576928297 2112 10-11 -0.016375911852122726 2112 12-13 0.006299026926122053 2112 14-15 -1.1522689179958547 2112 16-17 -1.1476890772852215 2112 18-19 0.16437297808599283 2112 20-21 -0.4785951992095647 2112 22-23 0.3209389720449778 2112 24-25 0.4307183906326415 2112 26-27 0.44978259111757524 2112 28-29 0.12343144227394731 2112 30-31 0.5332455809104246 2112 32-33 0.27061459559032386 2112 34-35 0.20849338252094185 2112 36-37 -0.5982911666957236 2112 38-39 -0.4525041785184314 2112 40-41 -2.0517826393449496 2112 42-43 0.15829903567448156 2112 44-45 0.3621790287819948 2112 46-47 0.5481204801004296 2112 48-49 -0.21210259570545986 2112 50-51 0.5098967254492806 2112 52-53 0.5833991975136499 2112 54-55 0.41233682778600667 2112 56-57 -0.5555747131925415 2112 58-59 0.2266231500614424 2112 60-61 -0.4077718927760756 2112 62-63 0.24711811347242474 2112 64-65 0.32047304753782413 2112 66-67 -0.031765494866405675 2112 68-69 -0.5807871626277716 2112 70-71 -0.27382026975151774 2112 72-73 -0.13348839612969243 2112 74-75 -0.33378103834972705 2112 76-77 0.4287880172217413 2112 78-79 0.571606797024927 2112 80-81 0.5180045955251558 2112 82-83 -0.018202900563544233 2112 84-85 0.24807533125642323 2112 86-87 0.505337869394765 2112 88-89 0.4725236556073824 2112 90-91 0.4186672365814701 2112 92-93 0.6062152151750979 2112 94-95 -0.4397628119617494 2112 96-97 0.3690744593888766 2112 98-99 0.4873645740806367 2112 100-101 0.3139674991608743 1113 1 0.3339190978524442 1113 2 7.783555491626558 1113 3 1.1838433218738302 1113 4 1.0007633230933344 1113 5 0.5669764759550695 1113 6 1.0008909812411844 1113 7 1.1244985014471567 1113 8 0.23661448947784436 1113 9 0.5009708794991639 1113 10-11 0.502372429536905 1113 12-13 0.3753998139860144 1113 14-15 -0.06423909005216899 1113 16-17 0.24067282943771318 1113 18-19 0.024275041401807584 1113 20-21 0.13912563427168578 1113 22-23 0.21927478652593635 1113 24-25 0.2326341359869346 1113 26-27 0.3496119458739031 1113 28-29 0.3224980949094558 1113 30-31 0.4428849012540823 1113 32-33 0.3841507927829184 1113 34-35 0.28407339633692175 1113 36-37 0.4144497856448055 1113 38-39 0.17929348631356135 1113 40-41 0.44479774195740873 1113 42-43 0.21522344338161759 1113 44-45 0.2257952166717132 1113 46-47 0.34793528835695753 1113 48-49 0.21768670245577226 1113 50-51 0.4150954394885247 1113 52-53 0.33660941327210736 1113 54-55 0.3044443592970225 1113 56-57 0.30479314524567513 1113 58-59 0.2718003026687157 1113 60-61 0.3555044900966635 1113 62-63 0.34072063668340746 1113 64-65 0.3790259224590926 1113 66-67 -0.06206275185526522 1113 68-69 0.37369260132027904 1113 70-71 -0.11644446684583443 1113 72-73 0.2879338671567311 1113 74-75 -0.3710833592478906 1113 76-77 0.2978108893008695 1113 78-79 0.3834982470367265 1113 80-81 0.28212330603022906 1113 82-83 -0.33656188314973434 1113 84-85 -0.017638132198960932 1113 86-87 0.3242641672652695 1113 88-89 0.3475683130750866 1113 90-91 0.21457842872005273 1113 92-93 0.3674575540633285 1113 94-95 -0.6156860733588161 1113 96-97 0.1434962091433576 1113 98-99 0.3895280765377578 1113 100-101 0.19618730922292826 2113 1 0.42543941449205747 2113 2 7.833456136758677 2113 3 1.191882819260229 2113 4 1.0989641046095961 2113 5 0.5915429691959311 2113 6 1.0796842843361176 2113 7 1.3868969325508829 2113 8 0.25007299600045485 2113 9 0.543458214981726 2113 10-11 0.45490855986743384 2113 12-13 0.28225135037319404 2113 14-15 0.015545332478623664 2113 16-17 0.2906203033330854 2113 18-19 0.12646929411964436 2113 20-21 0.19932285772056701 2113 22-23 0.16143987223881595 2113 24-25 0.15184536004375815 2113 26-27 0.3112700732668472 2113 28-29 0.32477783937702753 2113 30-31 0.4324533889082218 2113 32-33 0.31083857412954785 2113 34-35 0.3870941912341763 2113 36-37 0.37472823299427205 2113 38-39 0.2572471638339735 2113 40-41 0.48296505699060077 2113 42-43 0.2980319681005099 2113 44-45 0.21069508235581935 2113 46-47 0.4311956364506315 2113 48-49 0.39620684532853545 2113 50-51 0.38250261132881036 2113 52-53 0.3743859364796336 2113 54-55 0.2979159775433047 2113 56-57 0.2949600201410192 2113 58-59 0.14391903212776214 2113 60-61 0.31449344880582686 2113 62-63 0.18819402529979357 2113 64-65 0.4260915138615573 2113 66-67 0.39264159710707247 2113 68-69 0.6035077005208436 2113 70-71 0.02433704983457119 2113 72-73 0.3433585660708758 2113 74-75 -0.20726031913882537 2113 76-77 0.34222860175903236 2113 78-79 0.29533774254738887 2113 80-81 0.15309278698276785 2113 82-83 0.5194643982198812 2113 84-85 0.5392186821018754 2113 86-87 0.4036014784512787 2113 88-89 0.30876775910593324 2113 90-91 0.3512271313654267 2113 92-93 0.4196863043688879 2113 94-95 -0.4687041667288767 2113 96-97 0.11437565339968359 2113 98-99 0.44359767524910865 2113 100-101 0.18915709730658392 1112 1 0.21019649098624882 1112 2 7.800988291879584 1112 3 1.1247695189861773 1112 4 1.0931067181927254 1112 5 0.5521580295779849 1112 6 0.8577386055497485 1112 7 1.1465662202698255 1112 8 0.06586486428998484 1112 9 0.461272145941372 1112 10-11 0.40292897161383934 1112 12-13 0.09260770815482289 1112 14-15 0.2591743189862541 1112 16-17 0.2303984511287993 1112 18-19 0.15850406129526107 1112 20-21 0.19782545268797946 1112 22-23 0.21984499242584832 1112 24-25 0.06780627500544512 1112 26-27 0.30226725962522494 1112 28-29 0.33812495090187866 1112 30-31 0.3709518249939805 1112 32-33 0.2614570995197312 1112 34-35 0.22109360606855688 1112 36-37 0.27115807755905763 1112 38-39 0.2065453889020077 1112 40-41 0.40408501415389964 1112 42-43 0.29144616100179377 1112 44-45 0.33053718507473917 1112 46-47 0.41677837411968 1112 48-49 0.34687963073810124 1112 50-51 0.37789570710437204 1112 52-53 0.3095286606312939 1112 54-55 0.17502356925937335 1112 56-57 0.19477778002948298 1112 58-59 0.11939725071034246 1112 60-61 0.23752103164225957 1112 62-63 0.2392880558446464 1112 64-65 0.19363626827662017 1112 66-67 0.002498136520962646 1112 68-69 0.1681207415642163 1112 70-71 -0.04366219937621452 1112 72-73 0.010825572180522158 1112 74-75 0.2945307059858422 1112 76-77 0.44566780952202834 1112 78-79 0.270756158976198 1112 80-81 0.19189997835820805 1112 82-83 0.3701027020839689 1112 84-85 0.4768066573901777 1112 86-87 0.41187107532887524 1112 88-89 0.26272358669250195 1112 90-91 0.23790642020219366 1112 92-93 0.3259429561436349 1112 94-95 0.2805414122195202 1112 96-97 0.17962375163164523 1112 98-99 0.3213929711722514 1112 100-101 0.19544057100740275 2114 1 0.17892773441072407 2114 2 7.812207575491804 2114 3 1.1576762299204049 2114 4 1.0894613736220116 2114 5 0.5174312349232828 2114 6 0.8551021682810145 2114 7 1.1121951005833353 2114 8 0.044370698832878475 2114 9 0.3254259170151741 2114 10-11 0.38303624120859325 2114 12-13 0.36722036333279107 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-0.8743146554584911 2109 26-27 -0.7514895767537269 2109 28-29 -0.7567377619494451 2109 30-31 -1.159982313157741 2109 32-33 -1.0899712675368676 2109 34-35 -1.0238167975480224 2109 36-37 -0.7657344324382365 2109 38-39 -1.0021462795702973 2109 40-41 -1.0228371088081403 2109 42-43 -1.1733328689001823 2109 44-45 -0.8873055924181656 2109 46-47 -1.3129299731035715 2109 48-49 -0.9987270569220499 2109 50-51 -1.241583502625211 2109 52-53 -1.3344007308392278 2109 54-55 -1.1037565965313334 2109 56-57 -1.2466162105062608 2109 58-59 -0.9794020599525552 2109 60-61 -1.0494270695911752 2109 62-63 -0.5194440838781347 2109 64-65 -1.3839811685782664 2109 66-67 -0.9141814026521118 2109 68-69 -1.49713820116785 2109 70-71 -0.965296308332448 2109 72-73 -1.0251589818155011 2109 74-75 -0.9964297200197905 2109 76-77 -1.0122186730570988 2109 78-79 -0.9658550709842117 2109 80-81 -0.9179456375077706 2109 82-83 -0.7352262216691443 2109 84-85 -0.8207020883947038 2109 86-87 -1.1795388481995417 2109 88-89 -1.0643937890613984 2109 90-91 -1.1774176829086755 2109 92-93 -1.2875668826212632 2109 94-95 -0.8096623834191305 2109 96-97 -1.1048255170447163 2109 98-99 -1.012689838672813 2109 100-101 -0.9967464413687495 2108 1 -0.7117766924894369 2108 2 -22.869256692977537 2108 3 -3.234007102335685 2108 4 -3.4815580376707977 2108 5 -1.610813802469739 2108 6 -2.4344807056396824 2108 7 -3.5945996360686685 2108 8 -0.3145173435750266 2108 9 -0.2247424181114468 2108 10-11 -1.2462405141912427 2108 12-13 -0.9079846970815524 2108 14-15 -0.7867574556094752 2108 16-17 -0.25842295429515616 2108 18-19 -0.14774476759349398 2108 20-21 0.11941107595620082 2108 22-23 -0.37507234073154905 2108 24-25 -0.3838355943980716 2108 26-27 -0.25416647007956783 2108 28-29 -0.5989318681801947 2108 30-31 -1.1034314243348788 2108 32-33 -0.47514958145593056 2108 34-35 -0.6198439801459372 2108 36-37 -0.40057864550061595 2108 38-39 -0.4128739829818713 2108 40-41 -0.362724239654149 2108 42-43 -0.630473345469909 2108 44-45 -0.4782190882653481 2108 46-47 -1.1767296723307368 2108 48-49 -0.8537450410277216 2108 50-51 -0.9323723905334589 2108 52-53 -0.8350433724534767 2108 54-55 -0.6596587533243152 2108 56-57 -0.5526680215056956 2108 58-59 -0.07944317025798853 2108 60-61 -0.815951102401435 2108 62-63 -0.40559330072323263 2108 64-65 -1.8170080998520817 2108 66-67 -0.6760206786996719 2108 68-69 -1.0784846590697725 2108 70-71 -0.20260913232971944 2108 72-73 -0.04822947769623909 2108 74-75 -0.5654155373611331 2108 76-77 -0.869139636237442 2108 78-79 -0.8390121157513306 2108 80-81 -0.6457630939990793 2108 82-83 -0.45123008462481806 2108 84-85 -0.552162406843344 2108 86-87 -0.6442130411892393 2108 88-89 -0.3006050397365527 2108 90-91 -0.33124273594667386 2108 92-93 -0.5485065921915577 2108 94-95 -0.4231732148379379 2108 96-97 -0.4370159599584795 2108 98-99 -0.41151167799646515 2108 100-101 -0.34828837667614465 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 6.0 15 15.0 16 41.0 17 85.0 18 172.0 19 334.0 20 670.0 21 1909.0 22 6014.0 23 17951.0 24 43293.0 25 82324.0 26 129236.0 27 185767.0 28 239223.0 29 307220.0 30 409983.0 31 494678.0 32 608325.0 33 789361.0 34 1030963.0 35 1328901.0 36 2131638.0 37 5620593.0 38 2870566.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.46068743700637 48.68345007886244 1.044341377784573 0.8115211063466163 2 0.738315593653242 1.4296403249894083 4.597081308394834 93.23496277296252 3 0.832129393786273 55.20166917925394 43.00116422406209 0.9650372028977008 4 11.210478900034037 9.118047509863633 21.851103988228182 57.82036960187414 5 19.26506760917116 1.052955261549169 16.260313039824855 63.42166408945481 6 0.8532285008136562 48.44843952501425 43.32177371400973 7.3765582601623585 7 1.305807107411204 39.94027216436959 51.936933609533874 6.81698711868533 8 72.33593557698418 1.0747292455096755 1.132418952802052 25.45691622470408 9 54.327114567353576 1.2013484286533604 19.656760045911266 24.8147769580818 10-11 39.19639826770135 58.826411713703955 1.0249263954675756 0.9522636231271244 12-13 35.289965168987955 1.826879587475953 49.57408823512811 13.309067008407984 14-15 50.38507864279549 32.30268684458713 12.857537528679202 4.454696983938175 16-17 33.73935872457585 25.993517009475518 36.61316876316164 3.6539555027869963 18-19 39.361138794699244 7.495474643401163 41.179527203307536 11.963859358592055 20-21 4.003167504209391 30.322196064264972 27.743607872451697 37.931028559073944 22-23 27.698102761424625 13.357820731581322 24.889320182967726 34.05475632402633 24-25 45.878601599734466 22.146918565512223 21.01214057625596 10.962339258497353 26-27 14.797612383574526 80.70856617610067 2.0884772248667853 2.4053442154580194 28-29 37.457485820835636 13.44958252112917 19.338340838373846 29.75459081966135 30-31 37.149367689395625 26.57986849470786 28.590351419462518 7.680412396434 32-33 28.439893742467454 2.6275934600253215 49.83506621278943 19.097446584717794 34-35 51.56984043700613 26.495082478550568 17.019012755664857 4.916064328778446 36-37 24.999116524742092 24.52318104101362 15.472142675364317 35.00555975887997 38-39 13.507735853773797 29.074374047790798 44.014487154100635 13.403402944334768 40-41 14.785492207380111 36.56081058364094 20.57898551026954 28.074711698709415 42-43 17.738468377843716 13.530086136383549 37.07098993648058 31.660455549292156 44-45 23.81805501382194 21.453546915528566 32.75834634900004 21.970051721649455 46-47 32.920374092873374 27.124564735054356 18.904830572759465 21.0502305993128 48-49 30.62057142688862 39.70487815771849 16.477669426627013 13.196880988765876 50-51 49.58599981299774 15.906386102737866 18.614578274312688 15.89303580995171 52-53 28.098439758153553 21.481280631743708 18.703201886121512 31.717077723981223 54-55 30.055417212601203 28.34507660098601 13.239680456815606 28.359825729597183 56-57 23.77886847433885 20.565941979725714 37.54174727355854 18.113442272376894 58-59 23.521234926661585 37.392487116368876 20.119039091787407 18.967238865182132 60-61 27.35106263667792 39.03378973828763 9.297939024010159 24.317208601024294 62-63 20.81410343090254 32.116353323351696 27.138905869883235 19.930637375862524 64-65 23.91609856344469 23.18482952731374 38.93390181694049 13.965170092301078 66-67 39.857832879366114 21.217940584816446 32.72448799541181 6.199738540405618 68-69 30.535309929255718 38.99974526463397 13.856935170340165 16.608009635770145 70-71 10.083265702484308 22.510348317482723 34.74305103762942 32.66333494240355 72-73 18.939553571962666 25.342144976760462 21.497189981083505 34.22111147019337 74-75 41.501627557752904 20.556659354272842 29.296530371793384 8.645182716180875 76-77 18.9381602903885 32.43268348001886 16.78473843119826 31.84441779839438 78-79 30.506060149449652 29.416587910573654 32.780975194714266 7.2963767452624255 80-81 47.90181068254108 18.199393985055035 27.302195411474923 6.596599920928964 82-83 32.91945994139123 17.219751831800053 35.69302621442877 14.167762012379942 84-85 28.296598963830768 43.06327744288885 20.330704421818208 8.30941917146218 86-87 19.695651362993726 60.15548980481823 8.320290825330314 11.828568006857731 88-89 9.325553761064608 31.44142117302446 20.86648921902505 38.36653584688589 90-91 31.504715426484182 34.41706707319617 25.592535812037692 8.485681688281952 92-93 27.276347624936285 45.91465089107069 18.86420298138542 7.944798502607601 94-95 17.382170168623524 49.60640256973503 10.145701021665513 22.86572623997593 96-97 22.05838937061468 29.015425723412857 17.367184833085755 31.55900007288671 98-99 39.66911581550779 16.56071916849272 39.17968279311685 4.590482222882647 100-101 24.027312146778616 60.54292438163481 7.484599921910603 7.945163549675974 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 2.0 7 2.5 8 2.5 9 63.5 10 316.5 11 323.0 12 87.5 13 43.0 14 44.5 15 38.0 16 45.5 17 80.5 18 156.0 19 185.5 20 176.0 21 192.0 22 334.5 23 1108.0 24 2756.5 25 3213.0 26 2199.5 27 2892.0 28 5199.5 29 13128.0 30 15403.0 31 12453.0 32 47728.5 33 143266.5 34 138981.5 35 71617.0 36 109864.0 37 289587.0 38 321349.5 39 172542.5 40 176111.0 41 249283.5 42 386532.0 43 1024490.5 44 1287952.0 45 759181.0 46 449840.5 47 556380.5 48 947982.0 49 1924435.0 50 2744817.5 51 1919601.0 52 899592.5 53 686710.5 54 389976.0 55 153290.0 56 88294.5 57 65708.0 58 96224.0 59 78318.5 60 24194.5 61 15153.0 62 10314.5 63 4700.0 64 2109.5 65 921.0 66 550.5 67 304.5 68 132.0 69 146.5 70 151.5 71 85.0 72 43.5 73 31.5 74 22.5 75 20.0 76 15.0 77 13.0 78 10.5 79 7.0 80 5.0 81 4.0 82 4.0 83 5.0 84 5.0 85 5.0 86 4.0 87 3.5 88 2.5 89 2.0 90 2.0 91 2.5 92 3.0 93 1.0 94 0.5 95 2.5 96 4.0 97 4.0 98 8.0 99 26.5 100 83.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 18.790119899862987 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.067622423289193E-6 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 3.067622423289193E-6 40-41 0.0 42-43 0.0 44-45 3.067622423289193E-6 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.067622423289193E-6 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 3.067622423289193E-6 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.6299268E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 3.5160552005929255 #Duplication Level Percentage of deduplicated Percentage of total 1 74.38405832040849 2.6153845509867972 2 9.75799043681769 0.6861926604541776 3 3.3973275718309237 0.35835573831161566 4 1.8036358925688862 0.2536673344017158 5 1.256602952932326 0.2209142673869066 6 0.8170425284108619 0.17236599786747625 7 0.8132769298976984 0.20016686052223356 8 0.5452095980471003 0.15335896341013486 9 0.4606489853378806 0.14577005344905983 >10 5.052128185967265 4.4502794794055625 >50 0.9624229517818286 2.256953540931322 >100 0.5354604187445963 4.057499418991414 >500 0.09265766240173785 2.2500725444674226 >1k 0.09135088725912222 6.667120902434541 >5k 0.01111037439650614 2.71544554461604 >10k+ 0.019076303197296435 72.79645214236359 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTCGGGAG 1600565 9.819858167863735 No Hit ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGCTAAAATAGCTACC 1177299 7.223017622631887 No Hit GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGAATAA 941999 5.779394510231993 No Hit GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGCAAAG 769124 4.7187640573797545 No Hit ACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG 511039 3.135349391150572 No Hit ACACCATGGGAGTCGAACTCATTCGAAGCGGGGATGCTAAAATAGCTACC 450072 2.7613019185892274 No Hit ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGCTAAAGTAGCTACC 284180 1.7435138805006456 No Hit GCACCTACCGATTGAATGACTCGGTGAGAAATCGGGACCGTGGATCTGTT 241510 1.481722982897146 No Hit GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGCATAA 183862 1.1280383879815952 No Hit ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGCTTAACCTTCGGGAG 183525 1.1259708104682984 No Hit GCTGTTACCGATTGATTATTTTGATGAGTATTGGGGAGTTTAATAATAGG 171656 1.0531515893842596 No Hit GCTATTACCGATTGAGTGTAAAGGTGAACCTTCTCGATAGACGCAAGTTT 169404 1.039335017989765 No Hit GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACTGTATGTG 157354 0.9654053175884953 No Hit GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTCAGCAATGA 149823 0.9192007886489136 No Hit GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCTTTCAGCC 134512 0.825264054802952 No Hit ACACCATGGGAGTTTGTTGCACCAGAAGTAGGTAGTCTAACCGCAAGGAG 131782 0.8085148363717929 No Hit GCACCTACCGATTGAATGATTCGGTGAAACTTTCGGACCGTGGACAGGAC 131477 0.8066435866935865 No Hit GCTGCCCGGGACTGAGTTGATTCGAGAAGACCGTGGATTGTTGCTTAGGC 111576 0.6845460790018301 No Hit GCTTTTACCGATTGAGTGTCCAGGTGAGTCTTCTTGATAGTTGCAAAATT 109617 0.672527134347383 No Hit ACACCATGGGAATTTGTTGCACCAGAAGTAGGTAGTCTAACCGCAAGGAG 103601 0.6356175013503674 No Hit GCTGCCCGGAACTGAGCCATTTCGAGAAAAATCGGGACTGACTGCTTGTG 84176 0.5164403702055822 No Hit ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCCTTCGGGG 83571 0.5127285470734023 No Hit GCTCCTACCGATTTCGAGTGATGTGGTGAACTATCTGGACTGCGATTGGG 78464 0.4813958516419265 No Hit GCTGTCCGGGACTGAGCTGTTTCGAGAAGAGTGAGGACTGCTGCAGCGAG 77309 0.47430964384412844 No Hit GCACCTACCGATTGAATGACTCGGTGAGAAATCGGGACCGTGAGTCCGTT 74355 0.4561861305673359 No Hit GCACCTACCGATTGAATGACTCGGTGAAAAATTGGGACCGTGAAACTGTT 72655 0.44575621432815266 No Hit GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACCGTATGTG 66698 0.40920856077708523 No Hit ACACCATGGGAGTCTATTGCACCAGAAGTAGGTAGCCTAACGAAAGAGGG 65801 0.4037052461497044 No Hit GCTTTTACCGATTGAGTGTTCAGGTGAGTCTTCTTGATAGTTGCAAAGCT 65059 0.39915289447354324 No Hit GCTACTACCGATTGAATGGCTTAGTGAGGGTTTTGGAGAGCTTTTGAAAA 54122 0.3320517215865154 No Hit GCTATTACCGATTGAGTGTAAAGGTGAACCTTCTCGATAGTGTCACCGCT 53867 0.3304872341506379 No Hit ACACCATGGGAGTGGAGGATACCAGAAGTAGCTAGACTAACCGCAAGGGG 52634 0.3229224772548068 No Hit GCTATCCGGGACTGAGTTGTTTCGAGAGGAGTGGAGACTGTTGTTTTGGG 52211 0.3203272686847041 No Hit ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCCTCGGGAG 51247 0.3144128926526025 No Hit GCTGTTACCGATTGAATGGTTCAATGAGTAGTAAGGAGTTTGAGGCAGCA 51114 0.31359690508800764 No Hit GTTGTTTCCGATGATGGCGTAATGCAGGTGATCGGACTAGCAGACGTTTT 49224 0.30200129232797446 No Hit GTTGTTTCCGATGATGGTGGAATGCAGGTGATCGGACTGGCCTCGGCCGG 46445 0.28495144689933316 No Hit ACACCATGGGAGTGGGGGATACCAGAAGTGGGTAGGCTAACCGCAAGGAG 45627 0.279932816614832 No Hit GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCCTTACCGC 43619 0.2676132449629026 No Hit ACACCATGGGAGTGGGTTGTACCAGAAGTAGTTAGTCTAACCCTTCGGGG 41106 0.25219537466345116 No Hit GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCGGTAGCAATA 38890 0.23859967208343347 No Hit GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACGGCATGTG 37657 0.23103491518760227 No Hit ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTAGGGGG 35806 0.21967857697658572 No Hit ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAG 35775 0.21948838438634177 No Hit GCTATTACCGATTGGGTGTATAGGTGAACCTTCCTGATAGATGCAAGTCT 33959 0.2083467797449554 No Hit GCTTTTACCGATTGAGTGCTCAGGTGAACCTTCTCGATAGTGCAAACTAG 29727 0.18238242355423567 No Hit GCACCTACCGATTGAATGACTCGGTGAGAAATCGGGACCGTGGATCCGTT 26282 0.16124650505777313 No Hit GCTCCTACTGATTGAATTTTGTGATGAGGACCTTGGACTGTAGACGCATG 25517 0.15655304275014068 No Hit ACTCCTACCGATTCGAGTGGTTCGACGAATTCTCCGGATATTGCTCTCAT 25516 0.1565469075052941 No Hit GCTACCGCGAAAGGGTCCTAAGTAAAGCTTCACGGAGTGGCCGCACGGGG 25346 0.15550391588137577 No Hit GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCTCCGTGCC 24617 0.15103132238822012 No Hit GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGTCTGTCATGT 23608 0.14484086033802254 No Hit GCTACTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCGTGTTG 23529 0.14435617599514286 No Hit ACACCATGGGAGTTTGTTGCACCAGAAGTAGGTAGTCTAACCGTAAGGAG 22309 0.1368711772823172 No Hit GTTGTTTCCGATGATGGCGTAATGCAGGTGATCGGACGAGCAGGCGTTTT 22058 0.13533123082582604 No Hit GCTGTTACCGATTGAATGGTTCAATGAGTAGTAAGGAGTTTGGGGAATTT 21864 0.13414099332558985 No Hit GCACCTACCGATTGGACGATCCGGCAAGATGTTTGGAGTTGTGTCTGTTG 21850 0.13405509989773773 No Hit GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAGAA 21325 0.13083409635328408 No Hit GCTTTTACCGATTGAGTGTTCAGGTGAGTCTTCTTGATAGTGTCAAAGCT 19734 0.12107292180237787 No Hit GCTACCGGGGACTCTGAGCTGCGGCGAGAAGAATTTTTTTCTGATCGTCC 19180 0.11767399615737345 No Hit GCTATTACCGATTGGGTGTATAGGTGAACCTTCCTGATAGTCGCAAGATT 18329 0.11245290279293524 No Hit GCTGCTACCGATTGAATAGTAAATGTGAAATGGGAGGACTATCGTCTGAA 18123 0.1111890423545401 No Hit GCACCTACCGATTGAATGATTCGGTGAAACTTTCGGACCGTGGTTTGGAC 17493 0.1073238381011957 No Hit GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGTCTTTCATAT 17235 0.10574094493077849 No Hit GCACCTACCGATTGGACGATCCGGCAAGATGTTTGGAGTCGTGTTTCTTG 16712 0.10253221187601798 No Hit GCTGTTACCGATTGATTATTTTGATGAGTATTGGGGAGTTTTAATGGATT 16481 0.10111497031645839 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.5951636601103803E-4 0.0 0.0 2 1.5951636601103803E-4 0.0 0.0 3 1.5951636601103803E-4 0.0 0.0 4 1.5951636601103803E-4 0.0 0.0 5 1.5951636601103803E-4 0.0 0.0 6 1.6565161085761642E-4 0.0 0.0 7 1.6565161085761642E-4 0.0 0.0 8 1.717868557041948E-4 0.0 0.0 9 1.717868557041948E-4 0.0 0.0 10-11 1.779221005507732E-4 0.0 0.0 12-13 1.779221005507732E-4 0.0 0.0 14-15 1.779221005507732E-4 0.0 0.0 16-17 1.779221005507732E-4 0.0 0.0 18-19 1.840573453973516E-4 0.0 0.0 20-21 1.9019259024392998E-4 0.0 0.0 22-23 1.9019259024392998E-4 0.0 0.0 24-25 1.9632783509050835E-4 0.0 0.0 26-27 2.0246307993708674E-4 0.0 0.0 28-29 2.270040593234003E-4 0.0 0.0 30-31 2.454097938631354E-4 0.0 0.0 32-33 2.760860180960274E-4 0.0 0.0 34-35 2.9449175263576255E-4 0.0 0.0 36-37 3.2516797686865445E-4 0.0 0.0 38-39 3.681146907947032E-4 0.0 0.0 40-41 4.1719664956733026E-4 0.0 0.0 42-43 4.6014336349337894E-4 0.0 0.0 44-45 5.092253222660061E-4 0.0 0.0 46-47 6.012539949646819E-4 0.0 0.0 48-49 6.595388210071765E-4 0.0 0.0 50-51 7.086207797798036E-4 0.0 0.0 52-53 8.405285439812389E-4 0.0 0.0 54-55 9.785715530292527E-4 0.0 0.0 56-57 0.0011442231638868691 0.0 6.135244846578386E-6 58-59 0.0013160100195910639 0.0 6.135244846578386E-6 60-61 0.0014939321201418371 0.0 6.135244846578386E-6 62-63 0.0017546800261214186 0.0 6.135244846578386E-6 64-65 0.002015427932101 0.0 6.135244846578386E-6 66-67 0.002254702481117557 0.0 9.20286726986758E-6 68-69 0.0025768028355629222 0.0 1.2270489693156772E-5 70-71 0.002871294588198685 0.0 1.2270489693156772E-5 72-73 0.003227138789300231 0.0 1.2270489693156772E-5 74-75 0.0035799153679784883 0.0 1.2270489693156772E-5 76-77 0.00399404439512253 0.0 1.5338112116445964E-5 78-79 0.004454187758615908 0.0 1.840573453973516E-5 80-81 0.004837640561527057 0.0 1.840573453973516E-5 82-83 0.005319257281983461 0.0 1.840573453973516E-5 84-85 0.005797806380016575 0.0 1.840573453973516E-5 86-87 0.006374519395594943 0.0 1.840573453973516E-5 88-89 0.007000314369945939 0.0 1.840573453973516E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAGTAC 20 6.3259868E-6 116.51853 2 GCTATCC 41575 0.0 116.46248 1 CTACTAC 19880 0.0 116.45993 2 CACCATG 604675 0.0 116.42507 2 GCTATTA 35415 0.0 116.38693 1 ACACCAT 599490 0.0 116.37957 1 CTTGTAG 242155 0.0 116.37899 2 CTATCCG 41730 0.0 116.32308 2 CTGTCCG 11520 0.0 116.316246 2 GCTGTTA 39460 0.0 116.31184 1 GCTGTCC 11520 0.0 116.26568 1 GCTTGTA 242745 0.0 116.261734 1 CTCCTAC 38835 0.0 115.78344 2 CACCTAC 88315 0.0 115.7533 2 CTATTAC 35830 0.0 115.38034 2 CTGCTAC 15575 0.0 115.02231 2 GCACCAT 5825 0.0 114.91828 1 CTAGTAC 3400 0.0 114.80503 2 CTTTTAC 28570 0.0 114.78523 2 GCTTTTA 28770 0.0 114.716286 1 >>END_MODULE