FastQCFastQC Report
Sun 19 Apr 2015
000000000-AE2TW l01n01.33100000008a6b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE2TW l01n01.33100000008a6b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16299268
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTCGGGAG16005659.819858167863735No Hit
ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGCTAAAATAGCTACC11772997.223017622631887No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGAATAA9419995.779394510231993No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGCAAAG7691244.7187640573797545No Hit
ACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAG5110393.135349391150572No Hit
ACACCATGGGAGTCGAACTCATTCGAAGCGGGGATGCTAAAATAGCTACC4500722.7613019185892274No Hit
ACACCATGGGAGTTGAACTCATTCGAAGCGGGGATGCTAAAGTAGCTACC2841801.7435138805006456No Hit
GCACCTACCGATTGAATGACTCGGTGAGAAATCGGGACCGTGGATCTGTT2415101.481722982897146No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTTCTGCATAA1838621.1280383879815952No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGCTTAACCTTCGGGAG1835251.1259708104682984No Hit
GCTGTTACCGATTGATTATTTTGATGAGTATTGGGGAGTTTAATAATAGG1716561.0531515893842596No Hit
GCTATTACCGATTGAGTGTAAAGGTGAACCTTCTCGATAGACGCAAGTTT1694041.039335017989765No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACTGTATGTG1573540.9654053175884953No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCTCAGCAATGA1498230.9192007886489136No Hit
GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCTTTCAGCC1345120.825264054802952No Hit
ACACCATGGGAGTTTGTTGCACCAGAAGTAGGTAGTCTAACCGCAAGGAG1317820.8085148363717929No Hit
GCACCTACCGATTGAATGATTCGGTGAAACTTTCGGACCGTGGACAGGAC1314770.8066435866935865No Hit
GCTGCCCGGGACTGAGTTGATTCGAGAAGACCGTGGATTGTTGCTTAGGC1115760.6845460790018301No Hit
GCTTTTACCGATTGAGTGTCCAGGTGAGTCTTCTTGATAGTTGCAAAATT1096170.672527134347383No Hit
ACACCATGGGAATTTGTTGCACCAGAAGTAGGTAGTCTAACCGCAAGGAG1036010.6356175013503674No Hit
GCTGCCCGGAACTGAGCCATTTCGAGAAAAATCGGGACTGACTGCTTGTG841760.5164403702055822No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCCTTCGGGG835710.5127285470734023No Hit
GCTCCTACCGATTTCGAGTGATGTGGTGAACTATCTGGACTGCGATTGGG784640.4813958516419265No Hit
GCTGTCCGGGACTGAGCTGTTTCGAGAAGAGTGAGGACTGCTGCAGCGAG773090.47430964384412844No Hit
GCACCTACCGATTGAATGACTCGGTGAGAAATCGGGACCGTGAGTCCGTT743550.4561861305673359No Hit
GCACCTACCGATTGAATGACTCGGTGAAAAATTGGGACCGTGAAACTGTT726550.44575621432815266No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACCGTATGTG666980.40920856077708523No Hit
ACACCATGGGAGTCTATTGCACCAGAAGTAGGTAGCCTAACGAAAGAGGG658010.4037052461497044No Hit
GCTTTTACCGATTGAGTGTTCAGGTGAGTCTTCTTGATAGTTGCAAAGCT650590.39915289447354324No Hit
GCTACTACCGATTGAATGGCTTAGTGAGGGTTTTGGAGAGCTTTTGAAAA541220.3320517215865154No Hit
GCTATTACCGATTGAGTGTAAAGGTGAACCTTCTCGATAGTGTCACCGCT538670.3304872341506379No Hit
ACACCATGGGAGTGGAGGATACCAGAAGTAGCTAGACTAACCGCAAGGGG526340.3229224772548068No Hit
GCTATCCGGGACTGAGTTGTTTCGAGAGGAGTGGAGACTGTTGTTTTGGG522110.3203272686847041No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCCTCGGGAG512470.3144128926526025No Hit
GCTGTTACCGATTGAATGGTTCAATGAGTAGTAAGGAGTTTGAGGCAGCA511140.31359690508800764No Hit
GTTGTTTCCGATGATGGCGTAATGCAGGTGATCGGACTAGCAGACGTTTT492240.30200129232797446No Hit
GTTGTTTCCGATGATGGTGGAATGCAGGTGATCGGACTGGCCTCGGCCGG464450.28495144689933316No Hit
ACACCATGGGAGTGGGGGATACCAGAAGTGGGTAGGCTAACCGCAAGGAG456270.279932816614832No Hit
GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCCTTACCGC436190.2676132449629026No Hit
ACACCATGGGAGTGGGTTGTACCAGAAGTAGTTAGTCTAACCCTTCGGGG411060.25219537466345116No Hit
GCTTGTAGTAACGAATGGTCTGGTGAACCTTCTGGACTGCGGTAGCAATA388900.23859967208343347No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGACGGCATGTG376570.23103491518760227No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGTTAGTCTAACCTTAGGGGG358060.21967857697658572No Hit
ACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAG357750.21948838438634177No Hit
GCTATTACCGATTGGGTGTATAGGTGAACCTTCCTGATAGATGCAAGTCT339590.2083467797449554No Hit
GCTTTTACCGATTGAGTGCTCAGGTGAACCTTCTCGATAGTGCAAACTAG297270.18238242355423567No Hit
GCACCTACCGATTGAATGACTCGGTGAGAAATCGGGACCGTGGATCCGTT262820.16124650505777313No Hit
GCTCCTACTGATTGAATTTTGTGATGAGGACCTTGGACTGTAGACGCATG255170.15655304275014068No Hit
ACTCCTACCGATTCGAGTGGTTCGACGAATTCTCCGGATATTGCTCTCAT255160.1565469075052941No Hit
GCTACCGCGAAAGGGTCCTAAGTAAAGCTTCACGGAGTGGCCGCACGGGG253460.15550391588137577No Hit
GTTGTTTCCGATGGATGGCGAAATACAGGTTGTTGGACTGGCTCCGTGCC246170.15103132238822012No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGTCTGTCATGT236080.14484086033802254No Hit
GCTACTACCGATTGAATGGCTTAGTGAGCTCTCTGGACTGTTGCGTGTTG235290.14435617599514286No Hit
ACACCATGGGAGTTTGTTGCACCAGAAGTAGGTAGTCTAACCGTAAGGAG223090.1368711772823172No Hit
GTTGTTTCCGATGATGGCGTAATGCAGGTGATCGGACGAGCAGGCGTTTT220580.13533123082582604No Hit
GCTGTTACCGATTGAATGGTTCAATGAGTAGTAAGGAGTTTGGGGAATTT218640.13414099332558985No Hit
GCACCTACCGATTGGACGATCCGGCAAGATGTTTGGAGTTGTGTCTGTTG218500.13405509989773773No Hit
GCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAGAA213250.13083409635328408No Hit
GCTTTTACCGATTGAGTGTTCAGGTGAGTCTTCTTGATAGTGTCAAAGCT197340.12107292180237787No Hit
GCTACCGGGGACTCTGAGCTGCGGCGAGAAGAATTTTTTTCTGATCGTCC191800.11767399615737345No Hit
GCTATTACCGATTGGGTGTATAGGTGAACCTTCCTGATAGTCGCAAGATT183290.11245290279293524No Hit
GCTGCTACCGATTGAATAGTAAATGTGAAATGGGAGGACTATCGTCTGAA181230.1111890423545401No Hit
GCACCTACCGATTGAATGATTCGGTGAAACTTTCGGACCGTGGTTTGGAC174930.1073238381011957No Hit
GCTATCCGGGACTGAGCTGTTTCGAGAAGAGTGGGGACCGTCTTTCATAT172350.10574094493077849No Hit
GCACCTACCGATTGGACGATCCGGCAAGATGTTTGGAGTCGTGTTTCTTG167120.10253221187601798No Hit
GCTGTTACCGATTGATTATTTTGATGAGTATTGGGGAGTTTTAATGGATT164810.10111497031645839No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTAC206.3259868E-6116.518532
GCTATCC415750.0116.462481
CTACTAC198800.0116.459932
CACCATG6046750.0116.425072
GCTATTA354150.0116.386931
ACACCAT5994900.0116.379571
CTTGTAG2421550.0116.378992
CTATCCG417300.0116.323082
CTGTCCG115200.0116.3162462
GCTGTTA394600.0116.311841
GCTGTCC115200.0116.265681
GCTTGTA2427450.0116.2617341
CTCCTAC388350.0115.783442
CACCTAC883150.0115.75332
CTATTAC358300.0115.380342
CTGCTAC155750.0115.022312
GCACCAT58250.0114.918281
CTAGTAC34000.0114.805032
CTTTTAC285700.0114.785232
GCTTTTA287700.0114.7162861
Produced by FastQC (version 0.11.2)