FastQCFastQC Report
Mon 13 Apr 2015
000000000-ADVLY l01n02 heat 2h 3.34200000008239.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ADVLY l01n02 heat 2h 3.34200000008239.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844750
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG301.1396656E-437.5000049
TGACGCT458.6962973E-735.045
GGACTGC952.3646862E-1128.4210536
GACTGTG406.16478E-428.1250027
GTCGCGC602.4667577E-422.50000224
CTCAGCG602.4667577E-422.5000029
GTGTTAC500.002264116722.51
GTACACG500.002264116722.51
ACTGAGC1551.8189894E-1221.7741938
ACTGTCC754.6007786E-521.08
TAGACGG654.2369484E-420.7692325
CGTCGCG654.2369484E-420.76923223
GCGTGTC654.2369484E-420.76923225
GGTCCGT550.003935687720.45454611
GCCAGCG550.003935687720.45454615
GACTGCC550.003935687720.4545467
CTGTGAC550.003935687720.4545469
TATACTG2500.019.85
TAGCCCG807.559967E-519.68750218
GCATAGT1151.5940896E-719.5652181
Produced by FastQC (version 0.11.2)