FastQCFastQC Report
Mon 13 Apr 2015
000000000-ADVLY l01n02 heat 2h 1.3420000000821f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ADVLY l01n02 heat 2h 1.3420000000821f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202515
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCCA250.002107147636.010
GACGTGT352.820455E-432.14285713
GGACACG507.158672E-527.07
TAAACGT450.001226587925.04
CTGCCCG450.001226587925.013
GTTAGAC551.3682537E-424.5454563
CCGTGCG851.8938044E-723.8235289
GTACCGT1052.3665052E-923.571436
TAGACGC602.4676012E-422.5000025
GGTGAGC955.532165E-721.3157926
TAAGGTG2650.021.2264165
GTGTGAG855.032529E-621.176471
CTAGACG754.6028596E-521.0000024
GTGTTAG654.2383885E-420.769231
AGACCGA1403.086825E-919.2857136
GGACCAT706.983728E-419.2857136
TCGCGCA706.983728E-419.28571313
CCGAGCG951.3159146E-518.9473699
GCACTGA951.3159146E-518.9473696
CGTGTAC600.00651066718.7500023
Produced by FastQC (version 0.11.2)