FastQCFastQC Report
Mon 13 Apr 2015
000000000-ADVLY l01n02 drought 2h 3.342000000081f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ADVLY l01n02 drought 2h 3.342000000081f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347928
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT1500.024.045
GTGCACA1003.6456186E-822.50000211
GCGTGTT500.00226481122.5000027
CCGTGCG1056.1098035E-821.4285729
CCAGGGT1109.987343E-820.4545463
GACCGTG1109.987343E-820.4545467
ATCGCAT550.003936885420.45454610
ACAGTGA1351.9645086E-920.0000026
GCCTAGT706.9843256E-419.2857151
GACAGTG1202.4974543E-718.7499987
TGCAGTC1202.4974543E-718.7499985
GGTTACG600.006511067518.7499988
ACGTTCA851.2046367E-418.5294139
GAGACGT1102.2565673E-618.409096
TATACAC28600.018.4090945
CCTGGAC3050.017.7049183
AGACCGA1406.478513E-817.6785726
GTGCGCT901.8659787E-417.511
GTCCTAC1701.8317223E-917.2058831
CTGAGCG800.001711052516.8759
Produced by FastQC (version 0.11.2)