FastQCFastQC Report
Mon 13 Apr 2015
000000000-ADVLY l01n02 drought 2h 2.342000000081da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ADVLY l01n02 drought 2h 2.342000000081da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740774
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA300.005146091329.99999843
ACGCTAC300.005146091329.99999835
GTGTGAG608.186411E-626.2499981
GGTGGCC608.186411E-626.24999836
ATCGCAT709.300056E-725.71428510
ACGCACA450.001226142925.00000245
TGCGTCG450.001226142925.00000225
GTCGCGC551.3675619E-424.54545224
TTCGCAG551.3675619E-424.54545244
GCGTACA551.3675619E-424.54545222
GCGCTAT751.6962877E-624.0000021
GGTGTCT851.891749E-723.82352814
GTGTCTG851.891749E-723.82352815
GTCTAGG1353.6379788E-1223.3333321
ACAGTGA500.002263855422.56
TCGCAGA500.002263855422.545
CGTCGCG602.4663605E-422.49999823
TAGACGG855.0282233E-621.176475
GACGGTC855.0282233E-621.176477
GCCCTAG654.2362703E-420.76923227
Produced by FastQC (version 0.11.2)