FastQCFastQC Report
Mon 13 Apr 2015
000000000-ADVLY l01n02 control 2h 3.342000000081b0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ADVLY l01n02 control 2h 3.342000000081b0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108554
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCT300.0051471830.00000215
CTGTCCG507.158176E-527.09
TCAGGTG500.002264560222.53
AGACCGA1257.530616E-1021.66
GGTGTCC1401.364242E-1020.8928578
GACAGTG654.2381E-420.7692327
CCGTGCG654.2381E-420.7692329
TCAGGGC654.2381E-420.7692328
GCACTGA654.2381E-420.7692326
ACTGTCC1452.1827873E-1020.1724138
GTGTGAG908.250818E-620.01
CGTCATA1251.7671482E-819.80000111
AGTAGCG1051.4454745E-619.2857137
CTAGGGC1051.4454745E-619.2857133
GCGTGTC600.00651035318.75000225
GTGGCCT851.20439225E-418.52941137
GCGTGAT851.20439225E-418.52941111
AAGTGTC750.001110189618.042
GTCTAGA2150.017.7906991
TATACAC18850.017.66578545