FastQCFastQC Report
Mon 13 Apr 2015
000000000-ADVLY l01n02 control 2h 1.34200000008197.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ADVLY l01n02 control 2h 1.34200000008197.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671740
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGAC651.5225263E-524.2307683
GTCAGGG602.466028E-422.5000027
CGCCCTC602.466028E-422.50000218
CTCAGGA802.9717012E-622.5000022
GCGTCGT500.00226363822.526
TGCGTCG500.00226363822.525
CTGTGCC1305.0931703E-1122.4999989
TCTGTGC1459.094947E-1221.7241368
TTAGGAC1056.098344E-821.428573
TGACGCT654.2357045E-420.7692345
AGACGGC550.003934859820.4545466
CTACACT550.003934859820.4545464
CAGACTA550.003934859820.4545464
AGCCGCG1009.043415E-720.2519
GAGCGCG908.2428705E-620.07
TCGGCGG807.5570235E-519.68750232
ATAACAC807.5570235E-519.6875023
TAAGACT2550.019.4117644
GTCTAGG1051.4437428E-619.2857131
TCTAGCC1051.4437428E-619.2857133
Produced by FastQC (version 0.11.2)