FastQCFastQC Report
Mon 13 Apr 2015
000000000-ADVLY l01n01 heat 2h 3.3410000000823c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ADVLY l01n01 heat 2h 3.3410000000823c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844750
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG300.00514649530.0000029
TCGTCAC406.16478E-428.12500212
AGGACAG551.3677836E-424.5454545
GACCGTC751.6967588E-624.07
AACAGAC751.6967588E-624.07
CGTGCGA602.4667577E-422.50000210
GGCATGT802.9732055E-622.5000021
TTAGACG500.002264116722.54
ACTAGGC500.002264116722.53
GCGTGTC500.002264116722.525
CGCGTGT500.002264116722.524
TAGACGG855.0296003E-621.1764725
CGACACT754.6007786E-521.010
GTCCACG754.6007786E-521.01
CGAATGT550.003935687720.45454615
CCTAGAC1552.3646862E-1120.322583
CTACAGT1703.6379788E-1219.8529424
CACGGTA807.559967E-519.6875027
GTCCGGT807.559967E-519.6875026
GAGTGTC1151.5940896E-719.5652187
Produced by FastQC (version 0.11.2)