FastQCFastQC Report
Mon 13 Apr 2015
000000000-ADVLY l01n01 heat 2h 1.34100000008212.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ADVLY l01n01 heat 2h 1.34100000008212.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202515
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG302.165585E-645.0000049
GAGCGTC450.001226587925.09
CCAGGGT1003.644709E-822.53
GGTGGCC702.703128E-522.536
GTCGCAG1056.108348E-821.42857413
CTAGACC1950.020.769234
TAGACGG654.2383885E-420.769235
GCGCAGA654.2383885E-420.7692320
CCCGGTA654.2383885E-420.769239
GCGAGTC550.003936641420.45454638
GCCCTAG1452.1827873E-1020.1724131
ACTCCGT908.251773E-620.06
GTCTAGG2550.019.4117641
ACACCGC1403.086825E-919.2857136
GTGCGAG706.983728E-419.28571311
CCTAGAC2350.019.1489353
CCGGGCG951.3159146E-518.9473699
GTGGACA600.00651066718.7500024
ACTAGGC600.00651066718.7500023
GACGGTC600.00651066718.7500027
Produced by FastQC (version 0.11.2)