FastQCFastQC Report
Mon 13 Apr 2015
000000000-ADVLY l01n01 drought 2h 3.341000000081f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ADVLY l01n01 drought 2h 3.341000000081f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347928
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAC651.52400535E-524.2307681
CTAACCG654.2387558E-420.769239
CCGTGCG1009.057294E-720.2500029
AGCGCGC1351.9645086E-920.0000028
GCGTCGT1302.7734131E-819.0384626
TACGGGC851.2046367E-418.52941327
GTACCGT1354.2737156E-818.3333346
TCACGCG750.001110358618.024
TAGACGG750.001110358618.05
TATACAC29150.017.98456245
ACTATGC901.8659787E-417.58
GCGTCCG901.8659787E-417.59
GTGCTAG1953.45608E-1117.3076921
TAGAGTG1701.8317223E-917.2058835
CGTCCGG1053.113067E-517.14285710
TGCGTCG1459.671021E-817.06896425
GAGTGTC1853.074092E-1017.0270277
ACGGGCC1205.1744373E-616.87528
GGCTTAG1601.6141712E-816.8751
GGACTGC2301.8189894E-1216.6304346
Produced by FastQC (version 0.11.2)