FastQCFastQC Report
Mon 13 Apr 2015
000000000-ADVLY l01n01 drought 2h 2.341000000081dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ADVLY l01n01 drought 2h 2.341000000081dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740774
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGGG453.4891855E-530.00000227
CGTCGCA300.005146091329.99999810
CCGTGCG507.155021E-527.09
TAGACGG709.300056E-725.7142855
AGCCGTG450.001226142925.0000027
GACGGTC751.6962877E-624.0000027
ACTGCTC903.283676E-722.5000028
CCTAGAC903.283676E-722.5000023
GTCGAGT500.002263855422.512
TGCGGCT702.7013179E-522.512
AGTAGCG1003.6399797E-822.57
CCCGCGC500.002263855422.513
CGTCAGG602.4663605E-422.4999981
GTCTCAC955.5262535E-721.315791
ATAGACG855.0282233E-621.176474
GCTGGTC754.5997956E-521.0000028
CGTTGCT754.5997956E-521.00000214
GTGGCCT754.5997956E-521.00000235
GTACTGG754.5997956E-521.0000021
ACAGTGA654.2362703E-420.7692326
Produced by FastQC (version 0.11.2)