FastQCFastQC Report
Mon 13 Apr 2015
000000000-ADVLY l01n01 control 2h 3.341000000081b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ADVLY l01n01 control 2h 3.341000000081b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108554
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGAC2050.025.24393
TGCGTCG1001.3442332E-924.7525
TAGACGC651.5236876E-524.230775
GCGTCGT1104.0527084E-922.50000226
GGGTGTC500.002264560222.513
GTCGCAG702.7028813E-522.513
CGACACT654.2381E-420.76923210
AGCGCGC1201.1050361E-820.6250028
TACGGGC550.003936450520.45454627
CGTGTAC550.003936450520.4545463
GCCCTAG908.250818E-620.01
GACGGTC706.983255E-419.2857137
GCCGTGA706.983255E-419.2857138
TTGCGTC1302.771958E-819.03846424
CTAGACG951.3157631E-518.9473694
TAGACGG851.20439225E-418.5294115
AGCCGTG1102.2558324E-618.4090927
GAGCGCG1354.2717147E-818.3333327
TGGGTGT1002.0465293E-518.012
ATACAGC1002.0465293E-518.06
Produced by FastQC (version 0.11.2)