FastQCFastQC Report
Mon 13 Apr 2015
000000000-ADVLY l01n01 control 2h 1.3410000000819a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ADVLY l01n01 control 2h 1.3410000000819a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671740
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGTT300.005145753330.00000214
GGTGACT406.163454E-428.12500222
GTGGTCG406.163454E-428.1250029
CGGTGAT507.1540475E-527.010
CGCTCGG709.2978917E-725.71428510
GGGGACC450.00122602425.09
ACTGCTC450.00122602425.08
CCGCTCG1052.3628672E-923.5714269
TAGAGTG802.9717012E-622.5000025
CTGTCGA500.00226363822.532
GTACACG500.00226363822.51
GCGCCGA500.00226363822.512
AGTACCG903.2827302E-722.55
CCTAGAC1056.098344E-821.428573
TAAGGTG1601.8189894E-1221.0937525
ACTGTGC654.2357045E-420.769238
GTCTAGG1201.1032171E-820.6250021
CCCTGCG550.003934859820.4545469
GCCATAG550.003934859820.4545461
GGACACG550.003934859820.4545467
Produced by FastQC (version 0.11.2)