FastQCFastQC Report
Mon 15 Sep 2014
pBOB_4.3_GGCTAC_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamepBOB_4.3_GGCTAC_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1040391
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG11300110.86139730159142Illumina Single End PCR Primer 1 (100% over 50bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC81950.7876846301054122No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG76700.7372228325696781No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG45450.43685499009507006No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA45140.4338753410977219No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA31640.3041164331486912No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT29680.28527736206868376No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG28750.2763384150766394No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC25890.24884875013336333No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC21480.20646084020334662No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC19800.1903130649919117No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG19250.18502659096435858No Hit
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA18500.177817762744968No Hit
GGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCT18040.17339634810374177No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC17530.16849434491455617No Hit
TTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCC16490.1584981031170012No Hit
TGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATC15960.15340386450863186No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC15880.15263492283189686No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA15560.14955915612495685No Hit
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT15520.14917468528658936No Hit
GCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTC15400.14802127277148686No Hit
AAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC15250.14657950712760875No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG14690.14119691539046378No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT14680.14110079768087191No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC14450.1388900903602588No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG13670.13139290901209255No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCAT13290.12774043604760135No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG13220.12706761208045822No Hit
TTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT12980.12476078705025322No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT12140.11668689944453577No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGAT12130.11659078173494389No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA11530.1108237191594314No Hit
CTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGC11150.10717124619494017No Hit
CCGGCGCCTACAAGACCGACATCAAGCTGGACATCACCTCCCACAACGAG10800.10380712635922457No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG10750.10332653781126519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT120450.068.89580547
GGTCGCC122150.068.8825243
TGGTCGC122400.068.8562142
CGCCGTA121650.068.64776646
TGTAGGG125200.068.60223417
CCGTATC120850.068.55192648
GTAGGGA125650.068.5515318
GAGCGTC124300.068.53589
TCGTGTA124000.068.53226514
CGTGTAG124600.068.5112415
CGTATCA121900.068.3634149
GTCGCCG123200.068.3522744
AGCGTCG124700.068.3159610
GTGTAGG125800.068.30286416
GTGGTCG124750.068.2885841
TAGGGAA126350.068.1717419
CATTAAA123850.068.10657554
GTCGTGT125050.068.09675613
GTATCAT122700.068.0603150
ATCTCGG125500.067.9641435
Produced by FastQC (version 0.11.2)