##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename pBOB_4.3_GGCTAC_L001_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1040391 Sequences flagged as poor quality 0 Sequence length 76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.779795288502115 34.0 34.0 34.0 34.0 34.0 2 33.79560857408416 34.0 34.0 34.0 34.0 34.0 3 33.7272784943353 34.0 34.0 34.0 34.0 34.0 4 33.778641875987006 34.0 34.0 34.0 34.0 34.0 5 33.831498926845775 34.0 34.0 34.0 34.0 34.0 6 37.55984913364303 38.0 38.0 38.0 37.0 38.0 7 37.53898101771353 38.0 38.0 38.0 37.0 38.0 8 37.50084343290167 38.0 38.0 38.0 37.0 38.0 9 37.54724137367586 38.0 38.0 38.0 37.0 38.0 10-11 37.55024409092351 38.0 38.0 38.0 37.0 38.0 12-13 37.465992593169304 38.0 38.0 38.0 37.0 38.0 14-15 37.44714871620381 38.0 38.0 38.0 37.0 38.0 16-17 37.48770270023482 38.0 38.0 38.0 37.0 38.0 18-19 37.40125923811337 38.0 38.0 38.0 37.0 38.0 20-21 37.43335198016899 38.0 38.0 38.0 37.0 38.0 22-23 37.23451663845612 38.0 38.0 38.0 37.0 38.0 24-25 37.39652255738468 38.0 38.0 38.0 37.0 38.0 26-27 37.40014955915612 38.0 38.0 38.0 37.0 38.0 28-29 37.47113681298666 38.0 38.0 38.0 37.0 38.0 30-31 37.341020347157944 38.0 38.0 38.0 37.0 38.0 32-33 37.325557891215894 38.0 38.0 38.0 37.0 38.0 34-35 37.13565861296378 38.0 38.0 38.0 36.5 38.0 36-37 37.131540930284864 38.0 38.0 38.0 36.5 38.0 38-39 37.14398048426024 38.0 38.0 38.0 36.5 38.0 40-41 37.040562154036316 38.0 38.0 38.0 36.0 38.0 42-43 37.016705257927065 38.0 38.0 38.0 36.0 38.0 44-45 37.26886334080168 38.0 38.0 38.0 36.5 38.0 46-47 37.264192020115516 38.0 38.0 38.0 37.0 38.0 48-49 37.291005496971806 38.0 38.0 38.0 37.0 38.0 50-51 37.21264409246139 38.0 38.0 38.0 37.0 38.0 52-53 37.296516405851264 38.0 38.0 38.0 37.0 38.0 54-55 37.22860732167041 38.0 38.0 38.0 37.0 38.0 56-57 37.183051372032246 38.0 38.0 38.0 36.5 38.0 58-59 37.29054509314287 38.0 38.0 38.0 37.0 38.0 60-61 37.13446675336485 38.0 38.0 38.0 36.5 38.0 62-63 37.20029921442996 38.0 38.0 38.0 37.0 38.0 64-65 37.18651160957755 38.0 38.0 38.0 36.5 38.0 66-67 37.238988995483425 38.0 38.0 38.0 37.0 38.0 68-69 37.20775698751719 38.0 38.0 38.0 37.0 38.0 70-71 37.20210863031303 38.0 38.0 38.0 37.0 38.0 72-73 37.036132088801224 38.0 38.0 38.0 36.0 38.0 74-75 35.53328171812328 38.0 37.0 38.0 27.0 38.0 76 34.800109766424356 38.0 37.0 38.0 23.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.02430999459356542 1101 2 0.05006291103644145 1101 3 0.003168629108522225 1101 4 0.08145202884353608 1101 5 -0.007613634585851514 1101 6 0.059858427291068494 1101 7 -0.01658984958915255 1101 8 -0.029320383276726147 1101 9 0.02353814132937515 1101 10-11 0.02817291829085633 1101 12-13 0.0799122345458727 1101 14-15 0.017292691859914555 1101 16-17 0.06144172912431145 1101 18-19 0.03970767729784086 1101 20-21 -0.004883345902811698 1101 22-23 0.32001619744443843 1101 24-25 0.0778299603950856 1101 26-27 0.0638847919200316 1101 28-29 0.03531749468591272 1101 30-31 0.022840977341758162 1101 32-33 0.051002858625139424 1101 34-35 -0.05606208981772198 1101 36-37 0.026267422103373406 1101 38-39 0.05591963592814864 1101 40-41 0.05018818082439935 1101 42-43 0.06800138777973785 1101 44-45 0.07027058952258614 1101 46-47 0.06276467961643561 1101 48-49 0.022172792223656757 1101 50-51 0.10346601647444942 1101 52-53 -0.09497936345152169 1101 54-55 0.010955036489384895 1101 56-57 0.02389470189423548 1101 58-59 0.03447934380022133 1101 60-61 0.07843628691694704 1101 62-63 0.06577067425077843 1101 64-65 0.032420875033949415 1101 66-67 -0.025353820681822015 1101 68-69 0.11094281684042073 1101 70-71 -0.0074899616676233904 1101 72-73 0.21040549931935715 1101 74-75 0.07424279847859339 1101 76 0.021523860330489697 1102 1 0.03635068294927635 1102 2 0.04550843009310768 1102 3 0.04171358762345534 1102 4 0.05199038103725684 1102 5 -0.03944357184713709 1102 6 -0.015656989955822098 1102 7 0.0056960716475060735 1102 8 -0.09153134719974076 1102 9 0.04090411582905773 1102 10-11 0.05378066707998386 1102 12-13 0.0670212477348997 1102 14-15 0.034018402797556746 1102 16-17 0.08165917492222974 1102 18-19 0.04016050947996064 1102 20-21 -0.14713900128914048 1102 22-23 0.26846005984136667 1102 24-25 0.022917391546435795 1102 26-27 -0.05529680343593668 1102 28-29 -0.10476111220623352 1102 30-31 -0.008658542270090663 1102 32-33 0.15618849823218284 1102 34-35 0.13691026202195644 1102 36-37 0.13779709327813094 1102 38-39 0.1508035973640176 1102 40-41 0.11315090204357858 1102 42-43 0.10735245361157553 1102 44-45 0.12472023079975258 1102 46-47 0.12293681933864775 1102 48-49 0.05679083475432378 1102 50-51 0.13918845650593425 1102 52-53 -0.03996463434193487 1102 54-55 0.11115920462803075 1102 56-57 0.15011557733084402 1102 58-59 0.08952162887826631 1102 60-61 0.04507273937426248 1102 62-63 0.10298500965288326 1102 64-65 0.048248032825014775 1102 66-67 0.12769583771305548 1102 68-69 0.14874839919707483 1102 70-71 0.07823894195277603 1102 72-73 0.1783866068457911 1102 74-75 -0.2568334005044477 1102 76 -0.1745329186790272 1103 1 0.0032180344564949337 1103 2 0.05286862945769144 1103 3 -0.02229211056226177 1103 4 -0.005350181368328322 1103 5 0.04721911862429806 1103 6 0.10103393715266407 1103 7 0.13368627705600744 1103 8 0.0351204883614713 1103 9 0.08794779669675279 1103 10-11 0.09927271995515952 1103 12-13 0.121508414873027 1103 14-15 0.19522293822871717 1103 16-17 0.18318893127239022 1103 18-19 0.1448177308849381 1103 20-21 0.05994448262845964 1103 22-23 0.3892271649940042 1103 24-25 0.0994052732695252 1103 26-27 0.1117430810386395 1103 28-29 0.06789142355576416 1103 30-31 0.06661580364194464 1103 32-33 0.17608071200456266 1103 34-35 0.10996826438455543 1103 36-37 -0.02156402102232846 1103 38-39 0.054036670472314086 1103 40-41 0.017323441175584264 1103 42-43 0.11405188022265378 1103 44-45 0.07421420344773821 1103 46-47 0.1077629340025652 1103 48-49 0.011881661790091869 1103 50-51 0.09808607949635473 1103 52-53 -0.1251979622555197 1103 54-55 0.06688909973405543 1103 56-57 -0.042495313693919456 1103 58-59 -0.035155915302318874 1103 60-61 0.01058097957652393 1103 62-63 0.0949426539267364 1103 64-65 -0.18406720959061573 1103 66-67 0.003316700819695484 1103 68-69 -0.10093874513187018 1103 70-71 -0.03842129036696207 1103 72-73 -0.14444358624369613 1103 74-75 0.008179305083217514 1103 76 -0.20209876542238447 2116 1 -0.05097946911278228 2116 2 -0.04032707916964995 2116 3 0.01924237118416272 2116 4 0.02762666175724604 2116 5 0.022552219626945202 2116 6 -0.15925550191861504 2116 7 0.03245317567863282 2116 8 -0.03231536775838606 2116 9 0.012139292144446756 2116 10-11 0.03698854704617105 2116 12-13 0.06727549961002666 2116 14-15 0.14838557096011584 2116 16-17 0.12138896799562815 2116 18-19 0.15462726968354445 2116 20-21 0.018995449236548723 2116 22-23 0.4073817473421215 2116 24-25 0.10084416217923575 2116 26-27 0.07002136074230236 2116 28-29 -0.015471575658551728 2116 30-31 0.08166877129176697 2116 32-33 0.043049970657321524 2116 34-35 -0.066055395873029 2116 36-37 -0.1434502589264497 2116 38-39 -0.11272380283276107 2116 40-41 -0.10865937384796354 2116 42-43 -0.023358606884876565 2116 44-45 0.011336025919611359 2116 46-47 -0.09758104657159095 2116 48-49 0.036342835709596955 2116 50-51 -0.05688261206967127 2116 52-53 -0.16310365098485846 2116 54-55 -0.03870007715328683 2116 56-57 0.07106274337198926 2116 58-59 -0.13676355623399417 2116 60-61 -0.19635989975765966 2116 62-63 -0.0076026786621383735 2116 64-65 -0.05783832389106891 2116 66-67 0.09616066578023208 2116 68-69 -0.05678823231808394 2116 70-71 -0.006897263501542739 2116 72-73 0.05020618773351515 2116 74-75 -0.09778491099432074 2116 76 -0.3862779198635877 1117 1 -0.018916119532732978 1117 2 0.05929810332222729 1117 3 0.04094774672029189 1117 4 0.03635740082727068 1117 5 8.754628511056239E-4 1117 6 -0.012591163415414997 1117 7 -0.045072776620067145 1117 8 -0.05665266065059171 1117 9 0.01639176256121999 1117 10-11 -0.003331062730104861 1117 12-13 0.08809457609398663 1117 14-15 0.10091391866166788 1117 16-17 -0.02198944513823875 1117 18-19 0.15533964988188131 1117 20-21 0.01331607516791422 1117 22-23 0.4475948877656819 1117 24-25 0.10942014561817359 1117 26-27 0.17295768337028505 1117 28-29 0.09837183559681506 1117 30-31 0.10791066159240614 1117 32-33 -0.02478840520912229 1117 34-35 0.07718918988617673 1117 36-37 0.16306277836870464 1117 38-39 0.19191180596517654 1117 40-41 0.20354722082213783 1117 42-43 0.2655219331145773 1117 44-45 0.2037740543265656 1117 46-47 0.14664262858152455 1117 48-49 0.09631155679141301 1117 50-51 0.21084507097062755 1117 52-53 0.17019686112706012 1117 54-55 0.24693412466066178 1117 56-57 0.2047406575056243 1117 58-59 0.2942386929232086 1117 60-61 0.2426691552180955 1117 62-63 0.24929400130952217 1117 64-65 0.28504985113976034 1117 66-67 0.32457638416154566 1117 68-69 0.2137762380583439 1117 70-71 0.3294186195321984 1117 72-73 0.3384584387818208 1117 74-75 0.2558093909726864 1117 76 -0.05034549039559266 2117 1 0.028548524861612634 2117 2 0.03315647593956328 2117 3 0.018015281051098953 2117 4 -0.011807182079060397 2117 5 -0.03625458428970063 2117 6 -0.07208439973432945 2117 7 -0.006139593590759773 2117 8 -0.01894667581763798 2117 9 0.05826407246078702 2117 10-11 0.005744823722039882 2117 12-13 0.10374455559797724 2117 14-15 0.06269450894655648 2117 16-17 -0.007738574058102188 2117 18-19 0.13936019712517123 2117 20-21 -0.03166266022868314 2117 22-23 0.3946695956976285 2117 24-25 0.1540330273562347 2117 26-27 0.11784649255250201 2117 28-29 0.14022262469293878 2117 30-31 0.2024100467123091 2117 32-33 0.22000484135779885 2117 34-35 0.18525078037603038 2117 36-37 0.15896280911270821 2117 38-39 0.08525291069975083 2117 40-41 0.1243383665487201 2117 42-43 0.2275433514380012 2117 44-45 0.138211182836514 2117 46-47 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0.12058761400321316 2118 9 0.12448218241407716 2118 10-11 0.1677953473091236 2118 12-13 0.1383221568504851 2118 14-15 -0.023515002885481806 2118 16-17 -0.040375245686867345 2118 18-19 0.047114483352771686 2118 20-21 -0.005991557615878662 2118 22-23 0.27185390348470406 2118 24-25 0.0806867540643097 2118 26-27 0.07534289930779892 2118 28-29 0.0029139649732172757 2118 30-31 0.18453686258418145 2118 32-33 0.02156129379864069 2118 34-35 0.13450556709128847 2118 36-37 0.062074623187960754 2118 38-39 -0.06618124309112261 2118 40-41 -0.19362837027177449 2118 42-43 -0.005267977549216596 2118 44-45 -0.12453232365699307 2118 46-47 0.043157696752643915 2118 48-49 -0.24183448618008185 2118 50-51 0.0497159492431507 2118 52-53 -0.049078061272773255 2118 54-55 -0.01183845749034873 2118 56-57 -0.03317899042856709 2118 58-59 -0.06279740346236196 2118 60-61 -0.1458161452147877 2118 62-63 -0.012767920555702972 2118 64-65 -0.19794474455834887 2118 66-67 -0.0376969823684945 2118 68-69 -0.21919537788617305 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0.16555399881573862 1119 52-53 0.18177099609598457 1119 54-55 0.0724190762869128 1119 56-57 0.10832022377087469 1119 58-59 -0.023299395964691882 1119 60-61 0.08867905458532022 1119 62-63 -0.0035071432289299764 1119 64-65 0.1923735515442715 1119 66-67 -0.06076032990418412 1119 68-69 0.14904283497885729 1119 70-71 0.09824426358802896 1119 72-73 0.30643144621039653 1119 74-75 0.02622152397064781 1119 76 0.3406491612749747 2119 1 -0.05900664561900015 2119 2 -0.04942315812597542 2119 3 0.013650697186875504 2119 4 -0.02833258853133458 2119 5 -0.03924411081739976 2119 6 0.003382319596994421 2119 7 0.028960809039077162 2119 8 0.09504942529851235 2119 9 -0.05262247766407313 2119 10-11 0.030299191491266697 2119 12-13 0.0044877006976165035 2119 14-15 0.05394890474698144 2119 16-17 0.024568902625041744 2119 18-19 -0.12005388727419586 2119 20-21 0.10489631994561677 2119 22-23 0.29695681347450176 2119 24-25 0.16806983740669068 2119 26-27 0.13067649612010257 2119 28-29 0.14438602105398246 2119 30-31 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42-43 -0.15673902506400594 2104 44-45 -0.17155016562810488 2104 46-47 0.024112693881406244 2104 48-49 -0.04244204676991359 2104 50-51 -0.025807270841411878 2104 52-53 -0.1654934806581636 2104 54-55 -0.06641487438732696 2104 56-57 -0.25273470419136856 2104 58-59 -0.048628937374864734 2104 60-61 -0.11164626869480543 2104 62-63 -0.11638608289648289 2104 64-65 -0.5867002393682981 2104 66-67 -0.07126406690377252 2104 68-69 -0.42263354217469384 2104 70-71 -0.17651998248713596 2104 72-73 -0.5133941604753431 2104 74-75 0.030459426505473175 2104 76 -0.2553086837878382 2111 1 -2.2814270522530933E-4 2111 2 -0.03427128533508039 2111 3 -0.08730810539232436 2111 4 -0.026588440518168 2111 5 -0.014724068491695164 2111 6 -0.1065706271175344 2111 7 -0.049856849869449604 2111 8 0.035455835659547574 2111 9 0.0090296679239259 2111 10-11 -0.022612251622277313 2111 12-13 0.026130121355471658 2111 14-15 -0.13079522910367558 2111 16-17 -0.13798026998032498 2111 18-19 -0.03810165812733146 2111 20-21 0.017222365426185604 2111 22-23 0.2642705948874777 2111 24-25 0.037586565028391306 2111 26-27 -0.0010807286195273491 2111 28-29 0.0814513581522931 2111 30-31 -0.05678857602601539 2111 32-33 0.032356650701572676 2111 34-35 -0.05289464225426599 2111 36-37 -0.03638184387135368 2111 38-39 0.053686275539078565 2111 40-41 -0.1515811581609725 2111 42-43 -0.03133931126791367 2111 44-45 -0.1100452983949225 2111 46-47 0.04465436128420208 2111 48-49 -0.14396387423489188 2111 50-51 0.07924617155563851 2111 52-53 -0.10160817422563895 2111 54-55 0.10073633667922621 2111 56-57 -0.11228349135812721 2111 58-59 0.0891499789369945 2111 60-61 -0.2139362533245901 2111 62-63 0.0910315451444319 2111 64-65 -0.18639636854872776 2111 66-67 0.14362595659321187 2111 68-69 -0.07891041795866727 2111 70-71 0.08076390705934955 2111 72-73 -0.20743016563407224 2111 74-75 0.4658550769126961 2111 76 0.19717263345683023 2110 1 0.013828610629502691 2110 2 0.021592552058571357 2110 3 -0.006177294665867805 2110 4 -0.02365253185124061 2110 5 0.01593450710670652 2110 6 0.01684819206411703 2110 7 -0.035553959712444794 2110 8 0.09065999799635449 2110 9 0.06564681167279929 2110 10-11 -0.021896894316022042 2110 12-13 -0.001972745393707953 2110 14-15 -0.08434984913439081 2110 16-17 -0.03873428566357262 2110 18-19 -0.1658197144266893 2110 20-21 -0.09840448547660685 2110 22-23 0.1952331175416404 2110 24-25 -0.14035085096056577 2110 26-27 -0.04343399525238567 2110 28-29 -0.023998763674789814 2110 30-31 -0.2720639548958417 2110 32-33 -0.06449428756206288 2110 34-35 0.041685382359410994 2110 36-37 0.03385794446516144 2110 38-39 0.060198970737275204 2110 40-41 -0.13020733165551945 2110 42-43 0.03446575829943299 2110 44-45 0.08225600996060223 2110 46-47 0.12309114419790035 2110 48-49 0.07312702969726814 2110 50-51 0.17092398058325387 2110 52-53 -0.015715289849659086 2110 54-55 0.11014734615938693 2110 56-57 0.005113132949830401 2110 58-59 0.09313782105476065 2110 60-61 0.03906580018001904 2110 62-63 0.10264076215269569 2110 64-65 0.0847573889391171 2110 66-67 0.20833369571656135 2110 68-69 0.0757589110524961 2110 70-71 0.21078657008953172 2110 72-73 0.05174339629203217 2110 74-75 0.36512127707797504 2110 76 0.09263541678904375 2109 1 0.061097062060092355 2109 2 -0.04911955764870868 2109 3 0.06895460286743571 2109 4 -0.04183686598557301 2109 5 0.04997703040784529 2109 6 0.04774779259599171 2109 7 0.012723593016715995 2109 8 0.07546138429896132 2109 9 0.029452765834058425 2109 10-11 0.013602033434828797 2109 12-13 0.09563128876372673 2109 14-15 -0.11772521079458187 2109 16-17 -0.028887154044198837 2109 18-19 0.010539597563735015 2109 20-21 -0.1865923949454853 2109 22-23 0.08213643529756354 2109 24-25 -0.021310543804915483 2109 26-27 -0.18948690317943573 2109 28-29 -0.1638994269731029 2109 30-31 -0.7495708860576613 2109 32-33 -0.11692828962578261 2109 34-35 0.0463649965604489 2109 36-37 -0.011430235225951435 2109 38-39 -0.008981926439894039 2109 40-41 -0.24622749963301516 2109 42-43 -0.10510132417840623 2109 44-45 -0.03906892894612923 2109 46-47 0.07076601774414826 2109 48-49 -0.01661028506492812 2109 50-51 0.05233406724121892 2109 52-53 0.03292042851513344 2109 54-55 -0.05551434009201017 2109 56-57 0.012958217707740971 2109 58-59 0.18365236718745592 2109 60-61 -0.07643134341567048 2109 62-63 0.05725023938821039 2109 64-65 0.16821321318718674 2109 66-67 0.14331894131534995 2109 68-69 -0.028366842384286883 2109 70-71 0.10673302576697097 2109 72-73 -0.47369493340158186 2109 74-75 0.14021215309112733 2109 76 -0.39828569194397545 2108 1 0.01067913101906015 2108 2 -0.06483702571165395 2108 3 -0.05101236356379246 2108 4 -0.05047928179143213 2108 5 -0.039386841666924965 2108 6 -0.12253422395932034 2108 7 -0.16480210557541852 2108 8 0.007816134451886114 2108 9 -0.039651662065679716 2108 10-11 -0.03458022687986073 2108 12-13 0.031609555065323036 2108 14-15 -0.12091926542225195 2108 16-17 -0.04643197983204317 2108 18-19 -0.027151601106425005 2108 20-21 -0.023948134821203837 2108 22-23 0.07524299507975485 2108 24-25 0.012940425886114326 2108 26-27 -0.06076088999783735 2108 28-29 -0.11929266240623804 2108 30-31 -0.13604339954315492 2108 32-33 -0.07769157389734005 2108 34-35 -0.1491555794833488 2108 36-37 -0.3711507788280599 2108 38-39 -0.35204295059778445 2108 40-41 -0.4528289925649531 2108 42-43 -0.124670614685364 2108 44-45 -0.3457207176641788 2108 46-47 -0.42963238406990456 2108 48-49 -0.3423093518219389 2108 50-51 -0.23134498505942247 2108 52-53 -0.15088170398153267 2108 54-55 -0.25585440549468075 2108 56-57 -0.3744405730157254 2108 58-59 -0.19296744887765271 2108 60-61 -0.5195893576157502 2108 62-63 -0.23234230192678496 2108 64-65 -0.1883610169997212 2108 66-67 -0.28067481491578405 2108 68-69 -0.6099568536942073 2108 70-71 -0.2628810465556626 2108 72-73 -1.445185402887546 2108 74-75 -0.41631972421897956 2108 76 -0.8297642093755186 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 3.0 17 5.0 18 21.0 19 41.0 20 94.0 21 157.0 22 227.0 23 344.0 24 489.0 25 731.0 26 1077.0 27 1586.0 28 2421.0 29 3517.0 30 5452.0 31 8202.0 32 12317.0 33 18850.0 34 29721.0 35 54478.0 36 123693.0 37 776964.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.43866873127507 11.527589146772705 10.937906998426552 34.095835123525674 2 26.696886074562354 27.652584461034362 24.722243848706878 20.928285615696407 3 25.86729412307488 19.867818925769253 30.50189784417589 23.762989106979973 4 27.38999087842936 25.166403784730935 15.241961916241106 32.2016434205986 5 39.31070145743283 25.816832325539146 17.755343904359034 17.117122312668986 6 32.291513479067 29.538029452388574 18.79495305130475 19.375504017239674 7 17.69094503893248 28.132211831897813 33.98501140436625 20.191831724803464 8 21.091877957421776 29.860696603488496 24.946678700603908 24.10074673848582 9 32.37609706350785 17.914514831443178 27.607120784397406 22.102267320651563 10-11 29.24376508447305 31.587883785999686 18.6949906333292 20.473360496198065 12-13 21.84582527146044 27.178964447020398 23.34723195414032 27.627978327378845 14-15 22.02128815032041 27.394220057651403 22.18512078631976 28.399371005708428 16-17 28.823153987299005 21.67680227914313 21.3612958974078 28.138747836150063 18-19 22.511584586948562 21.87533340830515 28.48030211718479 27.132779887561504 20-21 28.500919365892248 21.043002102094306 28.13408612723486 22.321992404778587 22-23 22.739768029519674 34.617754286609554 21.58515404304728 21.057323640823498 24-25 22.863904051457578 22.59059334423308 27.84837623547301 26.697126368836333 26-27 23.579452340514287 21.26738889513654 21.23062387121765 33.92253489313152 28-29 28.63149527437281 28.02643429249196 21.195829260345388 22.146241172789846 30-31 21.837943619273908 21.75384062338102 27.088613800004037 29.31960195734104 32-33 21.80170724275777 27.472219578985207 22.15527623749148 28.57079694076554 34-35 34.16912487708948 21.544928781583078 21.580011745584113 22.705934595743333 36-37 22.774850993520705 21.84063491514248 27.18146350746979 28.20305058386703 38-39 22.88341594650473 27.39830506030906 21.226250515431218 28.492028477755 40-41 22.209630802265686 26.876241720660786 26.961930658761947 23.952196818311577 42-43 21.44837854229804 21.197271025989266 28.092467158981577 29.261883272731115 44-45 27.47688128790041 21.474618677016622 22.20544968189844 28.843050353184523 46-47 22.238861273723664 27.978196648277738 26.95963679022444 22.823305287774158 48-49 28.818876749222166 21.997210664067644 27.449343564102342 21.734569022607847 50-51 22.67142833799985 21.557616319249206 21.718036776558044 34.0529185661929 52-53 28.647258578745877 21.23840940569459 28.031528531100324 22.082803484459205 54-55 21.615479180423513 21.759992156794898 28.248466201649187 28.3760624611324 56-57 21.41050816471884 22.265475191538567 27.545269038275034 28.778747605467558 58-59 28.299696940861658 21.88071600004229 27.300024702251363 22.519562356844684 60-61 23.42032643559284 22.474958885570686 26.848082784420058 27.256631894416415 62-63 29.067877495821275 21.118398983457148 28.462046407641754 21.35167711307982 64-65 22.500755726581247 33.495820555666896 21.7055727686602 22.297850949091664 66-67 21.951768086967387 34.14902104018685 22.14131239246821 21.75789848037755 68-69 22.31723457815379 33.39480060861734 21.72909031316111 22.558874500067763 70-71 23.439697190767703 33.93094519272081 20.633636776942517 21.995720839568968 72-73 23.1723938403927 33.155803923717144 21.762779570373063 21.9090226655171 74-75 21.82064243154737 32.158246274717875 22.898458368055856 23.122652925678903 76 22.266436368634484 31.553810057949367 23.067769713501942 23.111983859914208 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 86.0 1 95.5 2 92.0 3 83.0 4 87.0 5 73.0 6 48.5 7 35.5 8 33.0 9 33.0 10 38.0 11 45.0 12 47.0 13 49.0 14 60.5 15 77.5 16 102.5 17 120.0 18 160.0 19 214.0 20 295.5 21 363.0 22 461.5 23 699.5 24 1318.5 25 1798.0 26 1949.5 27 2431.0 28 3077.0 29 3393.0 30 4096.5 31 5852.5 32 7865.5 33 8826.0 34 9653.5 35 12247.0 36 16319.5 37 18626.0 38 21438.5 39 28261.5 40 34886.0 41 40834.0 42 44168.0 43 48877.0 44 95944.0 45 104940.5 46 71579.0 47 61228.0 48 48220.0 49 42068.0 50 38573.0 51 37181.5 52 31900.5 53 32130.0 54 36249.0 55 36063.0 56 32766.5 57 23195.0 58 16734.0 59 18116.0 60 21413.0 61 27462.0 62 31596.0 63 31481.5 64 33189.5 65 32693.5 66 25813.0 67 21251.0 68 14923.5 69 9172.0 70 11933.5 71 14119.0 72 11816.0 73 8490.0 74 4798.0 75 2129.0 76 1313.0 77 273.0 78 32.5 79 16.0 80 10.0 81 2.5 82 1.0 83 1.0 84 0.5 85 0.5 86 1.0 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 4.80588547959373E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 1.922354191837492E-4 62-63 1.922354191837492E-4 64-65 1.4417656438781189E-4 66-67 9.61177095918746E-5 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 1040391.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.63071428012665 #Duplication Level Percentage of deduplicated Percentage of total 1 90.23947573841403 46.59128588638475 2 6.81082153368756 7.032951612375127 3 1.1768258136734262 1.822810720297507 4 0.49923566945846753 1.0310357683303155 5 0.2574400516239993 0.6645906874829881 6 0.13806240892816188 0.42769564729175596 7 0.08676639555731723 0.3135867684695407 8 0.07415869216500849 0.3063092997247536 9 0.07213057842738431 0.33517379570800915 >10 0.4454039611113029 4.55944334556652 >50 0.07962254072908855 2.911176125538272 >100 0.09897028682863025 11.228723210999599 >500 0.014000837058240612 4.782994970900661 >1k 0.006339652079310164 5.617013495271047 >5k 5.593801935256581E-4 2.0195659716225993 >10k+ 1.8646006450855272E-4 10.355642694036598 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC 107568 10.339189785378766 TruSeq Adapter, Index 11 (100% over 50bp) GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC 9118 0.8764012760587127 No Hit CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG 6552 0.6297632332459623 No Hit GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG 5308 0.5101928025136703 No Hit CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA 3718 0.35736564426258977 No Hit GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT 3444 0.3310293918344161 No Hit CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA 3087 0.29671536951011684 No Hit CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG 2602 0.2500982803580577 No Hit AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC 2346 0.2254921467025378 No Hit CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC 2128 0.20453848601150915 No Hit ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA 2095 0.20136660159497727 No Hit GGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCT 1833 0.17618376168190614 No Hit GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT 1765 0.16964775742965865 No Hit GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC 1745 0.16772540323782117 No Hit GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC 1735 0.16676422614190242 No Hit ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA 1732 0.16647587301312677 No Hit GCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTC 1717 0.16503410736924867 No Hit CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC 1708 0.1641690479829218 No Hit CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG 1671 0.16061269272802245 No Hit TTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCC 1648 0.15840198540740932 No Hit CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC 1631 0.15676798434434747 No Hit TGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATC 1580 0.15186598115516187 No Hit TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG 1500 0.14417656438781187 No Hit GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGAT 1483 0.14254256332475002 No Hit AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA 1457 0.14004350287536127 No Hit CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC 1433 0.13773667784515628 No Hit TTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT 1408 0.13533373510535943 No Hit AAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC 1394 0.1339880871710732 No Hit TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCAT 1361 0.13081620275454134 No Hit CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG 1317 0.12658702353249884 No Hit CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT 1281 0.12312678598719136 No Hit CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG 1162 0.11168877854575829 No Hit TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG 1135 0.10909360038677765 No Hit GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCC 1085 0.10428771490718393 No Hit CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT 1083 0.10409547948800019 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACACCTCGTATGC 1058 0.10169253674820332 TruSeq Adapter, Index 11 (98% over 50bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.003364119835715611 0.0 9.61177095918746E-5 2 0.003748590674083109 0.0 9.61177095918746E-5 3 0.004325296931634357 0.0 9.61177095918746E-5 4 0.0048058854795937295 0.0 9.61177095918746E-5 5 0.006055415704288099 0.0 9.61177095918746E-5 6 0.0063437688330637235 9.61177095918746E-5 9.61177095918746E-5 7 0.0070165928002068455 9.61177095918746E-5 9.61177095918746E-5 8 0.007977769896125591 9.61177095918746E-5 9.61177095918746E-5 9 0.009035064701636212 9.61177095918746E-5 9.61177095918746E-5 10 0.011245772022249327 9.61177095918746E-5 9.61177095918746E-5 11 0.012399184537351823 9.61177095918746E-5 9.61177095918746E-5 12 0.012783655375719322 9.61177095918746E-5 9.61177095918746E-5 13 0.014609891857964939 9.61177095918746E-5 9.61177095918746E-5 14 0.016051657501843057 9.61177095918746E-5 9.61177095918746E-5 15 0.016628363759394305 9.61177095918746E-5 9.61177095918746E-5 16 0.018935188789599294 9.61177095918746E-5 9.61177095918746E-5 17 0.019608012756742416 9.61177095918746E-5 9.61177095918746E-5 18 0.019992483595109915 9.61177095918746E-5 9.61177095918746E-5 19 0.022491544044498653 9.61177095918746E-5 9.61177095918746E-5 20 0.022779897173274277 9.61177095918746E-5 9.61177095918746E-5 21 0.023164368011641776 9.61177095918746E-5 9.61177095918746E-5 22 0.0234527211404174 9.61177095918746E-5 9.61177095918746E-5 23 0.023837191978784898 9.61177095918746E-5 9.61177095918746E-5 24 0.024029427397968647 9.61177095918746E-5 9.61177095918746E-5 25 0.02441389823633615 9.61177095918746E-5 9.61177095918746E-5 26 0.024606133655519898 9.61177095918746E-5 9.61177095918746E-5 27 0.02527895762266302 9.61177095918746E-5 9.61177095918746E-5 28 0.026432370137765514 9.61177095918746E-5 9.61177095918746E-5 29 0.028162488910419257 9.61177095918746E-5 9.61177095918746E-5 30 0.042868498477976066 9.61177095918746E-5 9.61177095918746E-5 31 0.043156851606751694 9.61177095918746E-5 9.61177095918746E-5 32 0.04354132244511919 9.61177095918746E-5 9.61177095918746E-5 33 0.04431026412185419 9.61177095918746E-5 9.61177095918746E-5 34 0.045271441217772934 9.61177095918746E-5 9.61177095918746E-5 35 0.04604038289450793 9.61177095918746E-5 9.61177095918746E-5 36 0.04671320686165105 9.61177095918746E-5 9.61177095918746E-5 37 0.047578266247977924 9.61177095918746E-5 9.61177095918746E-5 38 0.04873167876308042 9.61177095918746E-5 9.61177095918746E-5 39 0.049500620439815415 9.61177095918746E-5 9.61177095918746E-5 40 0.05007732669736666 9.61177095918746E-5 9.61177095918746E-5 41 0.05065403295491791 9.61177095918746E-5 9.61177095918746E-5 42 0.05103850379328541 9.61177095918746E-5 9.61177095918746E-5 43 0.051807445470020405 9.61177095918746E-5 9.61177095918746E-5 44 0.05315309340430665 9.61177095918746E-5 9.61177095918746E-5 45 0.054306505919409144 9.61177095918746E-5 9.61177095918746E-5 46 0.05536380072491977 9.61177095918746E-5 9.61177095918746E-5 47 0.056421095530430385 9.61177095918746E-5 9.61177095918746E-5 48 0.05738227262634913 9.61177095918746E-5 9.61177095918746E-5 49 0.058055096593492256 9.61177095918746E-5 9.61177095918746E-5 50 0.05949686223737037 9.61177095918746E-5 9.61177095918746E-5 51 0.060842510171656615 9.61177095918746E-5 9.61177095918746E-5 52 0.06218815810594286 9.61177095918746E-5 9.61177095918746E-5 53 0.062476511234718486 9.61177095918746E-5 9.61177095918746E-5 54 0.06420663000737223 9.61177095918746E-5 9.61177095918746E-5 55 0.0658406310704341 9.61177095918746E-5 9.61177095918746E-5 56 0.06853192693900659 9.61177095918746E-5 9.61177095918746E-5 57 0.07026204571166032 9.61177095918746E-5 9.61177095918746E-5 58 0.07276110616104907 9.61177095918746E-5 9.61177095918746E-5 59 0.07554851973921343 9.61177095918746E-5 9.61177095918746E-5 60 0.07785534476941842 9.61177095918746E-5 9.61177095918746E-5 61 0.07996993438043966 9.61177095918746E-5 9.61177095918746E-5 62 0.08170005315309341 9.61177095918746E-5 9.61177095918746E-5 63 0.08448746673125777 9.61177095918746E-5 9.61177095918746E-5 64 0.087370998019014 9.61177095918746E-5 9.61177095918746E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCACACG 12735 0.0 68.57086 11 ACGTCTG 12700 0.0 68.539375 15 ACACGTC 12695 0.0 68.538795 13 CACACGT 12735 0.0 68.46093 12 CACGTCT 12745 0.0 68.24245 14 AGCACAC 12810 0.0 68.1694 10 TATGCCG 12660 0.0 68.12006 46 GAGCACA 12830 0.0 68.11769 9 GTATGCC 12685 0.0 68.04099 45 CGTATGC 12700 0.0 67.98819 44 TCGGAAG 12905 0.0 67.91166 3 CTCGTAT 12620 0.0 67.892235 42 AGTCACG 12660 0.0 67.843605 28 CGGCTAC 12105 0.0 67.77364 33 ATGCCGT 12705 0.0 67.76859 47 AGAGCAC 12910 0.0 67.722694 8 TCGTATG 12730 0.0 67.69049 43 GCTACAT 11965 0.0 67.630585 35 ACGGCTA 12215 0.0 67.53582 32 GCCGTCT 12745 0.0 67.47352 49 >>END_MODULE