FastQCFastQC Report
Mon 15 Sep 2014
pBOB_4.2_TAGCTT_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamepBOB_4.2_TAGCTT_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1410566
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG32703223.184452198620978Illumina Single End PCR Primer 1 (100% over 50bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC121480.8612145762764735No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG107550.762459891986621No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA74500.5281567824547027No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG67990.4820050958267816No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA48780.34581862883409925No Hit
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA44150.3129949254412768No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG42030.2979654975378678No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC31920.22629214088529No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT31330.22210942274236015No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCAT30120.21353130587296165No Hit
GGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCT29930.21218432884388252No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC29470.20892322656295417No Hit
GCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTC26870.19049090932292426No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC25930.18382691770537501No Hit
TGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATC25490.18070760248013917No Hit
TTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT24100.17085340210950783No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG22470.159297757070566No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC22120.15681648359594658No Hit
TTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCC21500.15242108486947792No Hit
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT19980.14164526863684507No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGAT19870.14086543983053612No Hit
CTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGC19340.13710808285468384No Hit
CATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG19230.1363282540483749Illumina Single End PCR Primer 1 (98% over 50bp)
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG19030.13491038349144954No Hit
AAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC18960.13441412879652564No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG16620.11782504328049874No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG16050.11378411219326143No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC15950.11307517691479874No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA15740.11158641283002711No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC15380.10903424582756142No Hit
CCTCGCCCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGC14290.10130685129231812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC340050.068.744343
GAGCGTC344250.068.7291269
TGGTCGC340200.068.7242842
GCCGTAT339500.068.6907247
TCGGAAG346050.068.675053
TGTAGGG344300.068.6683117
AGCGTCG344950.068.66067510
TCGTGTA344050.068.62665614
GTGGTCG342450.068.6202441
CGCCGTA339550.068.6187646
GTCGTGT344550.068.5880113
GTAGGGA344500.068.57764418
CCGTATC339750.068.5680748
CGTGTAG344900.068.55900615
CGTATCA340500.068.53010649
ATCGGAA347350.068.5087052
CGTCGTG345500.068.4905912
GTGTAGG345900.068.4417516
AAGAGCG347050.068.416667
AGAGCGT346250.068.392788
Produced by FastQC (version 0.11.2)