FastQCFastQC Report
Mon 15 Sep 2014
pBOB_4.2_TAGCTT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamepBOB_4.2_TAGCTT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1410566
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC29595220.981081353158945TruSeq Adapter, Index 10 (100% over 50bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC138690.983222337699902No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG84880.6017442643591296No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG80680.5719689826636967No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA60120.4262118894117681No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA45830.32490503811944993No Hit
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA39620.280880157326917No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG37770.26776485467535727No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT36330.25755618666549457No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTCATCTCGTATGC34820.24685126396070795TruSeq Adapter, Index 10 (98% over 50bp)
GGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCT32920.23338149366991687No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCAT31430.22281835802082287No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC30360.21523275054127206No Hit
GCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTC29530.2093485877300318No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC28260.20034510969355562No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTACCTCGTATGC27160.19254682163046608TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGATTATCTCGTATGC26910.19077448343430933TruSeq Adapter, Index 10 (98% over 50bp)
TGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATC26630.18878946465461383No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC26080.18489032062306904No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGAT24500.17368914322335857No Hit
TTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT23880.16929374449688991No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG23690.1679467674678108No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCATATCTCGTATGC22190.15731273829087047TruSeq Adapter, Index 10 (98% over 50bp)
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT22120.15681648359594658No Hit
TTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCC22100.15667469654025407No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA21910.15532771951117494No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC19200.1361155734648361No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC19110.13547753171421967No Hit
AAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCC18690.1325000035446764No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC18440.13072766534851968No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG17480.12392188667527787No Hit
CTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGC15560.11031032932879425No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG14230.1008814901252405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG348750.068.5849463
GCACACG349200.068.3963311
AGCACAC349350.068.3769810
CGGAAGA349600.068.368134
GATCGGA350550.068.3426061
ATCGGAA350250.068.32122
CACACGT349700.068.29854612
ACACGTC349550.068.28779613
ACGTCTG349450.068.1971715
GAGCACA350800.068.144249
CACGTCT350050.068.1202614
AGAGCAC351200.068.076598
CTGAACT340950.067.803219
CGTCTGA341650.067.7872216
GTCTGAA341950.067.7379717
TATGCCG347600.067.6639846
CGTATGC347950.067.6462244
TCTGAAC342000.067.6359618
ACTCCAG349450.067.6062423
GTATGCC348150.067.5671445
Produced by FastQC (version 0.11.2)