##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	pBOB11_5a_GAGTGG_L001_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2121146
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.35962541003778	34.0	34.0	34.0	34.0	34.0
2	33.524821016563685	34.0	34.0	34.0	34.0	34.0
3	33.50969475934235	34.0	34.0	34.0	34.0	34.0
4	33.427624029651895	34.0	34.0	34.0	34.0	34.0
5	33.559464553595085	34.0	34.0	34.0	34.0	34.0
6	37.25400184617183	38.0	38.0	38.0	37.0	38.0
7	37.178842946218694	38.0	38.0	38.0	37.0	38.0
8	37.159474642481	38.0	38.0	38.0	37.0	38.0
9	37.223676258022785	38.0	38.0	38.0	37.0	38.0
10-11	37.327614176487614	38.0	38.0	38.0	37.0	38.0
12-13	37.25152394036054	38.0	38.0	38.0	37.0	38.0
14-15	37.20885950330623	38.0	38.0	38.0	37.0	38.0
16-17	37.29375252811452	38.0	38.0	38.0	37.0	38.0
18-19	37.31271515492097	38.0	38.0	38.0	37.0	38.0
20-21	37.31451088232493	38.0	38.0	38.0	37.0	38.0
22-23	37.355905722661234	38.0	38.0	38.0	37.0	38.0
24-25	37.36211415904421	38.0	38.0	38.0	37.0	38.0
26-27	37.3192877812277	38.0	38.0	38.0	37.0	38.0
28-29	37.297802697221215	38.0	38.0	38.0	37.0	38.0
30-31	37.22033773252761	38.0	38.0	38.0	37.0	38.0
32-33	37.15245768089514	38.0	38.0	38.0	37.0	38.0
34-35	37.26787288569481	38.0	38.0	38.0	37.0	38.0
36-37	37.10744192054672	38.0	38.0	38.0	36.0	38.0
38-39	37.093821924563414	38.0	38.0	38.0	36.0	38.0
40-41	37.251950125073904	38.0	38.0	38.0	37.0	38.0
42-43	37.17928185990026	38.0	38.0	38.0	36.5	38.0
44-45	37.151856119286464	38.0	38.0	38.0	36.5	38.0
46-47	37.11274612874361	38.0	38.0	38.0	36.0	38.0
48-49	37.042564491081706	38.0	38.0	38.0	36.0	38.0
50-51	37.143650884946155	38.0	38.0	38.0	36.5	38.0
52-53	37.11932936252384	38.0	38.0	38.0	36.0	38.0
54-55	37.12455978984945	38.0	38.0	38.0	36.5	38.0
56-57	37.04180546742185	38.0	38.0	38.0	36.0	38.0
58-59	37.20216312314192	38.0	38.0	38.0	37.0	38.0
60-61	37.19346593775252	38.0	38.0	38.0	36.5	38.0
62-63	37.24086295804249	38.0	38.0	38.0	37.0	38.0
64-65	37.18870860374533	38.0	38.0	38.0	37.0	38.0
66-67	37.25505882197642	38.0	38.0	38.0	37.0	38.0
68-69	37.330234222443906	38.0	38.0	38.0	37.0	38.0
70-71	37.350633572606505	38.0	38.0	38.0	37.0	38.0
72-73	37.3525815761857	38.0	38.0	38.0	37.0	38.0
74-75	37.35180770206294	38.0	38.0	38.0	37.0	38.0
76	36.88005964700214	38.0	38.0	38.0	35.0	38.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.011546288173853725
1101	2	0.09782379595887392
1101	3	0.02523101997995525
1101	4	0.0537706794025965
1101	5	0.028516545253530978
1101	6	0.07557916739038006
1101	7	0.14624427117968253
1101	8	0.24112383907052504
1101	9	0.05951254301506026
1101	10-11	0.1072094355328872
1101	12-13	0.13585596257897237
1101	14-15	0.12660799537469813
1101	16-17	-0.0038348033703812234
1101	18-19	-0.014099494197004958
1101	20-21	0.006135744187737657
1101	22-23	-0.01870813620394074
1101	24-25	0.015539491029784358
1101	26-27	-0.0012453380605137454
1101	28-29	0.1168982510488803
1101	30-31	-0.02726225007872074
1101	32-33	0.04289150119107177
1101	34-35	8.402027004521528E-4
1101	36-37	0.0961347837974742
1101	38-39	0.08386837967758964
1101	40-41	0.07913697738242575
1101	42-43	0.011813299480365913
1101	44-45	-0.012945071106372552
1101	46-47	0.07011928565890457
1101	48-49	0.09773613456133745
1101	50-51	0.020221080434218663
1101	52-53	0.014075122548476315
1101	54-55	0.12995620535205887
1101	56-57	0.1520206117855878
1101	58-59	0.029208923753408556
1101	60-61	0.14389767564161104
1101	62-63	0.08322511589146586
1101	64-65	0.20191760578104123
1101	66-67	0.023428562802941144
1101	68-69	0.022900614723077695
1101	70-71	0.02164815234337425
1101	72-73	0.032675664468648336
1101	74-75	0.07072659050906083
1101	76	-0.16812972972791584
1102	1	0.05183961126719794
1102	2	0.05796476423953578
1102	3	0.12579315529890067
1102	4	0.024226175427735086
1102	5	0.07240001618753666
1102	6	0.12992210577100138
1102	7	0.047813154478802744
1102	8	0.23966536030658858
1102	9	0.20799068841522939
1102	10-11	0.17068296151697382
1102	12-13	0.18543497472101222
1102	14-15	0.13009397316418614
1102	16-17	0.04281731041747605
1102	18-19	0.033187789301614146
1102	20-21	0.15672570154772814
1102	22-23	0.08820563485956256
1102	24-25	0.11148098300790821
1102	26-27	0.06086611062960401
1102	28-29	0.18489577345133057
1102	30-31	-0.0034878435092338123
1102	32-33	0.13426125623929153
1102	34-35	0.10481437324281018
1102	36-37	0.17237484665295
1102	38-39	0.11000072570278263
1102	40-41	0.08550278800894517
1102	42-43	0.06714019557639261
1102	44-45	0.049943296114136615
1102	46-47	0.08174563429767545
1102	48-49	0.11477090634450349
1102	50-51	0.08991848413583625
1102	52-53	0.11077592184000196
1102	54-55	0.12056230857759687
1102	56-57	0.2345941469005126
1102	58-59	0.10826991084555715
1102	60-61	0.27004663880114776
1102	62-63	0.13312475036360638
1102	64-65	0.2756910550857654
1102	66-67	0.14261729074044638
1102	68-69	0.17562275660051085
1102	70-71	0.10906477212861176
1102	72-73	0.14104687112295977
1102	74-75	0.16519420157877107
1102	76	0.15892578770068866
1103	1	-0.015057511927771827
1103	2	0.11079557573832233
1103	3	0.04396156399266715
1103	4	0.0327883430714806
1103	5	0.034697862758356735
1103	6	0.03692432201906115
1103	7	-0.1318936043984138
1103	8	0.22918008668001022
1103	9	0.1150639520928749
1103	10-11	0.03279769429038737
1103	12-13	0.1049572210743932
1103	14-15	-0.00961347967943027
1103	16-17	0.056387893276870216
1103	18-19	-0.021499223596507022
1103	20-21	0.09536203978733937
1103	22-23	0.02014610206593659
1103	24-25	0.027766587198676973
1103	26-27	-0.027288051118020462
1103	28-29	0.1254299637728451
1103	30-31	0.019089953426160378
1103	32-33	0.05244591885796979
1103	34-35	0.07665713086847603
1103	36-37	0.1718830860595446
1103	38-39	0.017492797787411973
1103	40-41	0.05826295588659036
1103	42-43	0.09050038725079901
1103	44-45	0.0869529275805121
1103	46-47	0.025400308361405166
1103	48-49	0.1089548107429863
1103	50-51	-9.902893137834212E-4
1103	52-53	0.021940841273234923
1103	54-55	-0.049097715186150026
1103	56-57	0.17701885599017686
1103	58-59	0.12563368993897228
1103	60-61	0.24211684026474245
1103	62-63	0.028276458530477555
1103	64-65	0.23541282916104223
1103	66-67	0.037245984429006285
1103	68-69	0.13662987645454194
1103	70-71	0.003142022843242387
1103	72-73	0.02917664563404543
1103	74-75	0.03749903416271394
1103	76	0.06201525247799111
2116	1	-0.10690479023566013
2116	2	0.08125122637503068
2116	3	-0.09073784827810272
2116	4	-0.02113240369899927
2116	5	-0.015964130289525258
2116	6	-0.05364047065287991
2116	7	-0.33689123497639883
2116	8	0.1346212384018841
2116	9	-0.013392240288631285
2116	10-11	-0.06884222528145045
2116	12-13	-0.06323757469711921
2116	14-15	-0.16469809552475567
2116	16-17	-0.04870455845228605
2116	18-19	-0.21887935054289187
2116	20-21	-0.029686681512160362
2116	22-23	-0.0810597291470998
2116	24-25	-0.21404848746072958
2116	26-27	-0.17836790737540298
2116	28-29	-0.004970195019211587
2116	30-31	-0.06688033295294105
2116	32-33	-0.2547043472743198
2116	34-35	-0.10059204764440466
2116	36-37	0.039391302828043706
2116	38-39	-0.2140328095837063
2116	40-41	0.04821757732833731
2116	42-43	-0.19360019020766828
2116	44-45	-0.3142566887628533
2116	46-47	-0.19894460321232543
2116	48-49	-0.009737468786454428
2116	50-51	-0.1609123301751012
2116	52-53	-0.07078681976983603
2116	54-55	-0.22406659774673443
2116	56-57	0.027432276335758843
2116	58-59	-0.08307134456263299
2116	60-61	0.06522182599286452
2116	62-63	-0.1985888217560472
2116	64-65	0.026183663204541574
2116	66-67	-0.13302966855657417
2116	68-69	0.024675081472317117
2116	70-71	-0.18158806890490098
2116	72-73	-0.06563111369971608
2116	74-75	-0.11997381867389123
2116	76	-0.14518904267414
1117	1	-0.07770845195265963
1117	2	0.05473302343673225
1117	3	0.01858957804910233
1117	4	0.0878081688888841
1117	5	-0.06830539053432716
1117	6	-0.25945153840784485
1117	7	-0.9489256237775834
1117	8	-0.11336559843396543
1117	9	-0.1165544623293755
1117	10-11	-0.09745495674824411
1117	12-13	-0.059803455806559214
1117	14-15	-0.30029034699514057
1117	16-17	-0.01231638994244122
1117	18-19	-0.18770467923710044
1117	20-21	0.0871724500639246
1117	22-23	-0.07595655114933209
1117	24-25	-0.00441961489228504
1117	26-27	-0.0801394960283659
1117	28-29	0.05115845900157012
1117	30-31	-0.10346615736733611
1117	32-33	0.02679180987927765
1117	34-35	-0.07208502621102753
1117	36-37	0.08059767257190487
1117	38-39	-0.31932307565050877
1117	40-41	0.09569489307023815
1117	42-43	-0.21636118866031495
1117	44-45	-0.04744190344862176
1117	46-47	-0.14198002579871627
1117	48-49	0.10503849088293293
1117	50-51	-0.13644870960043676
1117	52-53	0.10291956913931699
1117	54-55	-0.23377961385451584
1117	56-57	0.14728230177016144
1117	58-59	-0.2425094132134049
1117	60-61	0.024494794388260743
1117	62-63	-0.47160090133692734
1117	64-65	-0.013964006457840128
1117	66-67	-0.23544308215561216
1117	68-69	0.08979604169068978
1117	70-71	-0.42421798564898694
1117	72-73	-0.12128193429405343
1117	74-75	-0.25694321496981587
1117	76	0.09613799870000861
2117	1	0.024390662592132628
2117	2	0.026906700079415202
2117	3	0.008457751657871881
2117	4	0.045773955237329744
2117	5	-0.032954094819174884
2117	6	-0.3335095263028265
2117	7	0.058530522224614856
2117	8	0.19269499912168442
2117	9	0.01899808828351013
2117	10-11	0.05625024217395236
2117	12-13	-0.0028377183190784194
2117	14-15	-0.011199034131450958
2117	16-17	0.09648289696804113
2117	18-19	-0.10773044733986836
2117	20-21	0.13023303116248286
2117	22-23	0.02102914470553685
2117	24-25	0.08402654178405555
2117	26-27	-0.01158712223350733
2117	28-29	0.1298262658851499
2117	30-31	0.15817626081955893
2117	32-33	0.1318626729592225
2117	34-35	0.09987574161809931
2117	36-37	0.1789933040693441
2117	38-39	-0.050560845258139864
2117	40-41	0.16608346026625753
2117	42-43	-0.011967579471289014
2117	44-45	0.09687686999555467
2117	46-47	0.012584545761370691
2117	48-49	0.2229552127786576
2117	50-51	0.05166920468159475
2117	52-53	0.19606869001816563
2117	54-55	-0.20175080042471194
2117	56-57	0.25810136155493524
2117	58-59	0.17753989235482237
2117	60-61	0.1589197951569048
2117	62-63	-0.1456013436371535
2117	64-65	0.18700797923801105
2117	66-67	-0.016403364819524313
2117	68-69	0.1807531164978542
2117	70-71	8.735680750575625E-4
2117	72-73	0.13144289410889343
2117	74-75	-0.0019034385668064147
2117	76	0.27214893284141795
1116	1	0.02321838748809313
1116	2	-0.017091728262784045
1116	3	0.0859589222842061
1116	4	-0.0026204730379006946
1116	5	0.09300542560704628
1116	6	0.08157694277868899
1116	7	0.28563710426459465
1116	8	0.2924073776264038
1116	9	0.16205281170369545
1116	10-11	0.11989895889860946
1116	12-13	0.13390583258249222
1116	14-15	0.08985005252635148
1116	16-17	0.1886432770880333
1116	18-19	0.12242020706942469
1116	20-21	0.22287605684218903
1116	22-23	0.12234907345840185
1116	24-25	0.10794422607970233
1116	26-27	0.11245570366607183
1116	28-29	0.19766526684244923
1116	30-31	0.09866532136084771
1116	32-33	0.1455135964984251
1116	34-35	0.06826984521256207
1116	36-37	0.23621080517401793
1116	38-39	0.22894033348521248
1116	40-41	0.27469272574199266
1116	42-43	0.08151574791801863
1116	44-45	0.18430509145015606
1116	46-47	0.07287433855924519
1116	48-49	0.23413044552150097
1116	50-51	0.02260672544975506
1116	52-53	0.2192025746661983
1116	54-55	0.14965765456666702
1116	56-57	0.2755410305429109
1116	58-59	0.07713921600421969
1116	60-61	0.26131028261940514
1116	62-63	0.1565219036574632
1116	64-65	0.2891394196486985
1116	66-67	0.07006565327991154
1116	68-69	0.15056585940599376
1116	70-71	0.06077120739227837
1116	72-73	0.1570669914076035
1116	74-75	0.1576382187356984
1116	76	0.2555368193066414
2118	1	0.09984084732121801
2118	2	-0.03939239473077549
2118	3	0.08311616256393961
2118	4	0.0020512169731716767
2118	5	0.102869252013285
2118	6	0.1512339520376429
2118	7	0.3901775846179376
2118	8	0.3157349932220299
2118	9	0.09541938583107168
2118	10-11	0.15736523588700635
2118	12-13	0.1642638792863309
2118	14-15	0.15565861698925687
2118	16-17	0.14107771630840205
2118	18-19	0.2154465043952456
2118	20-21	0.1976057593698286
2118	22-23	0.16275100967232703
2118	24-25	0.17956584675918918
2118	26-27	0.15261744482621253
2118	28-29	0.1439038969127182
2118	30-31	0.18453674058641667
2118	32-33	0.1880503516180525
2118	34-35	0.12561496844329412
2118	36-37	0.19431067231647603
2118	38-39	0.2176141360845847
2118	40-41	0.2190735083123343
2118	42-43	0.2035569232350838
2118	44-45	0.11722153950800873
2118	46-47	0.1403147843196848
2118	48-49	0.20474743957427677
2118	50-51	0.14492786688631298
2118	52-53	0.242404966862523
2118	54-55	0.22102383725802355
2118	56-57	0.28396655457977715
2118	58-59	0.22258363532193925
2118	60-61	0.23679902623527482
2118	62-63	0.2507884244314056
2118	64-65	0.14396938899423617
2118	66-67	0.15025348616055823
2118	68-69	0.15472074739355435
2118	70-71	0.18982579623804696
2118	72-73	0.2254439903077028
2118	74-75	0.2263120587293841
2118	76	0.2706341376783641
1119	1	0.09363785432316263
1119	2	0.06087888617022941
1119	3	0.09302334385237998
1119	4	-0.02337784030007839
1119	5	0.10120637689759349
1119	6	0.1946859676544861
1119	7	0.3990420129398444
1119	8	0.13347415623712777
1119	9	0.10477319280153097
1119	10-11	0.1981579989289841
1119	12-13	-0.036383754990211514
1119	14-15	0.1521056710594948
1119	16-17	0.11545211672465427
1119	18-19	0.22582303998288467
1119	20-21	0.1518956266631193
1119	22-23	0.25211737156186587
1119	24-25	0.14982519593176846
1119	26-27	0.19994279596251374
1119	28-29	0.1141872088730409
1119	30-31	0.2011355343044272
1119	32-33	0.16603261466309505
1119	34-35	0.13489101893552657
1119	36-37	0.14009463571664327
1119	38-39	0.2617948418869105
1119	40-41	0.15509688258033094
1119	42-43	0.2810895273362348
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2101	26-27	-0.08960309991820736
2101	28-29	-0.23497505052458223
2101	30-31	-0.05989204018997896
2101	32-33	-0.25622302663022367
2101	34-35	0.008020304947983448
2101	36-37	-0.5407982352920087
2101	38-39	-0.1671656443384535
2101	40-41	-0.09798649129168524
2101	42-43	0.011337385201059647
2101	44-45	-0.18769087165399867
2101	46-47	-0.15158425068128878
2101	48-49	-0.15569942910336465
2101	50-51	-0.029229390673499722
2101	52-53	-0.15537345819275572
2101	54-55	0.04345784151909271
2101	56-57	-0.6710088263174896
2101	58-59	-0.10458069974752249
2101	60-61	-0.22426203645493104
2101	62-63	-0.0673661881646268
2101	64-65	-0.4850233167178075
2101	66-67	-0.12577428965040838
2101	68-69	-0.13809493097991776
2101	70-71	-0.02992269059233621
2101	72-73	-0.07337344376870192
2101	74-75	0.016774853565493686
2101	76	-0.22346966020371895
2107	1	-0.05059795894362651
2107	2	-0.01567719412410895
2107	3	0.02018655542548231
2107	4	0.08111993493659497
2107	5	0.12174897959775421
2107	6	0.055104360176372325
2107	7	0.03872501661464156
2107	8	0.027628010018020177
2107	9	-0.02906718440663525
2107	10-11	0.06060782863085734
2107	12-13	0.10135455290927808
2107	14-15	0.05853487306004013
2107	16-17	0.06326758982981318
2107	18-19	0.18978454807385958
2107	20-21	-0.07927708069470185
2107	22-23	-0.028349127713795497
2107	24-25	-0.049974952392290106
2107	26-27	0.10684657900679184
2107	28-29	-0.09115425623791396
2107	30-31	0.08815616402262805
2107	32-33	0.03769664888888258
2107	34-35	0.14116011448730603
2107	36-37	0.022807277073589205
2107	38-39	0.19906735511184337
2107	40-41	0.06934683145934883
2107	42-43	0.20162314523386016
2107	44-45	0.062096406881195776
2107	46-47	-0.038798222352710354
2107	48-49	-0.07394573395433213
2107	50-51	0.07295629692806216
2107	52-53	-0.02086986061365792
2107	54-55	0.16090735101227693
2107	56-57	-0.12387745179071885
2107	58-59	0.14579600134266713
2107	60-61	-0.057703337994894355
2107	62-63	0.0986207038175877
2107	64-65	-0.1863938682269719
2107	66-67	0.08968782957230559
2107	68-69	-0.05515557549024663
2107	70-71	0.07853784025029142
2107	72-73	0.022002672419418445
2107	74-75	0.09975313351170456
2107	76	0.11519057242512076
2106	1	0.0027119982638765805
2106	2	0.03514924379776119
2106	3	-0.0024509870389621824
2106	4	0.028240777360821312
2106	5	-0.028932141636474284
2106	6	0.04257300842374434
2106	7	0.14232673842906962
2106	8	-0.0560988033928993
2106	9	-0.040052109214329334
2106	10-11	0.14006381001158985
2106	12-13	0.024785175270345405
2106	14-15	-0.034357961106451285
2106	16-17	0.019765693885560154
2106	18-19	0.17064387555797111
2106	20-21	0.13871377136761254
2106	22-23	-0.05247645236157439
2106	24-25	-0.02970447889597949
2106	26-27	0.058989091836949115
2106	28-29	-0.039070387787646155
2106	30-31	-0.08045052057161683
2106	32-33	-0.024053793688871394
2106	34-35	0.08510360999844835
2106	36-37	0.08686448399075886
2106	38-39	0.16093944169125507
2106	40-41	0.04427680821985547
2106	42-43	0.19399300435159716
2106	44-45	0.05600477432227535
2106	46-47	-0.12142328267397318
2106	48-49	-0.016101733727552414
2106	50-51	0.07117289358701129
2106	52-53	0.052920629827170274
2106	54-55	0.17710954948780255
2106	56-57	0.09298814085356355
2106	58-59	0.15261381808154795
2106	60-61	0.09796545015256442
2106	62-63	0.07024455624961945
2106	64-65	-0.03141185414487069
2106	66-67	0.10786055041334919
2106	68-69	0.015944779530173037
2106	70-71	0.10305552299810472
2106	72-73	0.05515805746804858
2106	74-75	0.13556817726907155
2106	76	-0.08523449785098336
2105	1	0.026289193298147495
2105	2	-0.15830570660605048
2105	3	-0.16260901052116594
2105	4	-0.02388982404172424
2105	5	-0.09164574450152685
2105	6	-0.16261547375280117
2105	7	-0.1868226339800998
2105	8	-0.08950305232655609
2105	9	-0.12992812997986647
2105	10-11	-0.1845318963675524
2105	12-13	-0.1756544979260255
2105	14-15	-0.09909291639148421
2105	16-17	-0.04788691953599766
2105	18-19	-0.0500514639894476
2105	20-21	0.03441074041153769
2105	22-23	-0.10304655468998902
2105	24-25	-0.07386548716628738
2105	26-27	-0.09075086299459656
2105	28-29	-0.1690910880123795
2105	30-31	-0.2850216119680624
2105	32-33	-0.1277274853254582
2105	34-35	-0.04951959157285302
2105	36-37	-0.05524944366302975
2105	38-39	-0.012898800007299371
2105	40-41	-0.08501260173185443
2105	42-43	0.054680091969991906
2105	44-45	-0.0327845243080489
2105	46-47	-0.16349638697787583
2105	48-49	-0.12965695005991051
2105	50-51	-0.11219922484836076
2105	52-53	0.016240183944034925
2105	54-55	-0.05072424080967863
2105	56-57	0.018567051717923277
2105	58-59	-0.05706941374484131
2105	60-61	0.07784787784720493
2105	62-63	-0.12131528639852718
2105	64-65	-0.11277841603017436
2105	66-67	-0.07246781395819824
2105	68-69	-0.12999040008992324
2105	70-71	-0.09095110948240404
2105	72-73	-0.0882364296907241
2105	74-75	0.013287668136364061
2105	76	-0.021659443307470383
2104	1	0.08189131289888252
2104	2	-0.02415353459164038
2104	3	-0.02116434779077281
2104	4	-0.10670012282185581
2104	5	-0.02913126833850299
2104	6	-0.0210262340109324
2104	7	0.06343961493386274
2104	8	0.07747742249060963
2104	9	-0.047626722587480685
2104	10-11	-0.13188295505035796
2104	12-13	-0.1771425908685984
2104	14-15	-0.20265006555074905
2104	16-17	-0.07269393876467234
2104	18-19	-0.15033442795012064
2104	20-21	-0.0028308207956868614
2104	22-23	-0.06053518603012975
2104	24-25	0.03038512292079787
2104	26-27	-0.03960475130060104
2104	28-29	-0.15155621040838696
2104	30-31	-0.07079268055562693
2104	32-33	-0.19856972863422584
2104	34-35	-0.06356167894193732
2104	36-37	-0.0822741363991426
2104	38-39	-0.11182620268768773
2104	40-41	-0.3596703587348884
2104	42-43	-0.15198598479183545
2104	44-45	-0.13776105540567585
2104	46-47	-0.20790734330935123
2104	48-49	-0.11284546624059999
2104	50-51	-0.13521138858332193
2104	52-53	-0.1286956115549458
2104	54-55	-0.12561122704394734
2104	56-57	-0.08630066097299505
2104	58-59	-0.24223322934518166
2104	60-61	-0.10387155866282427
2104	62-63	-0.20218592292565063
2104	64-65	-0.15682149689440905
2104	66-67	-0.13728520006425526
2104	68-69	-0.11090687458622028
2104	70-71	-0.12963179452866314
2104	72-73	-0.0902062709589515
2104	74-75	-0.09740728582398361
2104	76	0.05089593091872047
2111	1	0.08034522936378607
2111	2	-0.05298566963687534
2111	3	-0.017581387069398602
2111	4	-0.06877689964282041
2111	5	-0.0877943144327844
2111	6	0.015480061856997906
2111	7	-0.006889129744429567
2111	8	-0.1405340105340045
2111	9	-0.015473119981841421
2111	10-11	-0.13372759187188876
2111	12-13	-0.1309427027204677
2111	14-15	-0.09545925101288333
2111	16-17	-0.06139053624033863
2111	18-19	-0.29358586906209894
2111	20-21	-0.093218780068554
2111	22-23	0.008932372576992975
2111	24-25	-0.06779598475574744
2111	26-27	-0.17129598747570185
2111	28-29	-0.16471710111983384
2111	30-31	-0.022492413411079326
2111	32-33	-0.18066723020988462
2111	34-35	-0.07461754810115195
2111	36-37	-0.06297782705751587
2111	38-39	-0.17891600163675747
2111	40-41	-0.19972456001175232
2111	42-43	-0.19422522664865483
2111	44-45	-0.09401079817756397
2111	46-47	-0.1776068754907243
2111	48-49	-0.1923192747880904
2111	50-51	-0.1556444815307927
2111	52-53	-0.08132960655905208
2111	54-55	-0.24320438667329825
2111	56-57	-0.12150652715872923
2111	58-59	-0.21427917991683643
2111	60-61	-0.006001903830906485
2111	62-63	-0.10102699320408703
2111	64-65	0.05337736610029964
2111	66-67	-0.01993073848619531
2111	68-69	-0.013227741248904579
2111	70-71	-0.09992558991827138
2111	72-73	-0.11599761226786853
2111	74-75	-0.06334989798676816
2111	76	-0.1956509633768988
2110	1	0.030348277593205353
2110	2	-0.011709711693740132
2110	3	-0.021403305660932403
2110	4	0.016750964483946973
2110	5	0.05407425461483939
2110	6	0.020166170826499297
2110	7	0.09046157746686134
2110	8	0.23145825862660985
2110	9	0.3059412685562606
2110	10-11	0.14350308949344281
2110	12-13	0.15453466860501663
2110	14-15	0.07530648565274589
2110	16-17	0.028973121627402065
2110	18-19	-0.06599733754141823
2110	20-21	0.049458704586747615
2110	22-23	0.13444457441555357
2110	24-25	0.03989435132304919
2110	26-27	0.08918394820823039
2110	28-29	0.06963304631423739
2110	30-31	0.15842911268072157
2110	32-33	0.23434750310912733
2110	34-35	0.16406264547496363
2110	36-37	0.18848037402563733
2110	38-39	0.13972252797051254
2110	40-41	0.06283430281143154
2110	42-43	-0.07134458932345922
2110	44-45	0.05236071813279608
2110	46-47	0.1769612162489409
2110	48-49	0.10920414012157664
2110	50-51	0.12852207233773072
2110	52-53	0.016429790356802698
2110	54-55	0.06678626563330425
2110	56-57	0.08407112429492258
2110	58-59	0.09747895685403307
2110	60-61	-0.023293859966408093
2110	62-63	0.13327344318762613
2110	64-65	0.15548564387463415
2110	66-67	0.14323986331751826
2110	68-69	0.04789329350740701
2110	70-71	0.009841025406579718
2110	72-73	0.03402021057510041
2110	74-75	0.10375069742983811
2110	76	-0.06279131501838009
2109	1	-0.08333731559703494
2109	2	-0.09947297685819478
2109	3	-0.04955066815927722
2109	4	0.08383921368332636
2109	5	0.027141090116046485
2109	6	0.11316197565143682
2109	7	-0.07329352686391388
2109	8	0.16030937205195528
2109	9	0.1509577666838382
2109	10-11	0.033185743504731136
2109	12-13	0.13182715696700598
2109	14-15	-0.0017178820900767278
2109	16-17	0.05001746314813005
2109	18-19	0.005598453014940219
2109	20-21	-0.03162256855654988
2109	22-23	-0.043350079144914844
2109	24-25	-0.11409944638364067
2109	26-27	-0.04080004970583673
2109	28-29	0.007976655329166249
2109	30-31	-0.02833430045653529
2109	32-33	0.12515598506680448
2109	34-35	0.0020716922794647985
2109	36-37	0.17278855515504432
2109	38-39	0.019402058940890754
2109	40-41	0.02651057228464282
2109	42-43	-0.03786005192132791
2109	44-45	0.09496620193388594
2109	46-47	0.08608265402456539
2109	48-49	0.009957557413152074
2109	50-51	-0.18197438978126712
2109	52-53	-0.08337229679986535
2109	54-55	-0.05191214720135662
2109	56-57	-0.08720942946047217
2109	58-59	-0.12130571746803298
2109	60-61	-0.23791570300340936
2109	62-63	-0.019873429489024375
2109	64-65	-0.05707600912646882
2109	66-67	0.04226952810095952
2109	68-69	-0.08159680864234531
2109	70-71	-0.06929167484698695
2109	72-73	-0.09370561604021788
2109	74-75	-0.11917449407800262
2109	76	-0.09318653923511278
2108	1	0.019980028059762844
2108	2	0.006845118262361893
2108	3	-0.023761031925580767
2108	4	0.01813164923166255
2108	5	-0.006460676156294198
2108	6	-0.20014743889504416
2108	7	-0.13738353425173244
2108	8	0.12674367109123352
2108	9	0.14407915908942925
2108	10-11	-0.06732149066785809
2108	12-13	0.1746094782213916
2108	14-15	0.043427428483752806
2108	16-17	0.19067321464514464
2108	18-19	0.05623294422726133
2108	20-21	0.05469508425358782
2108	22-23	0.021083646746653528
2108	24-25	0.010801285083360312
2108	26-27	-0.13207062042702944
2108	28-29	-0.007927744994560726
2108	30-31	-0.07427312521299001
2108	32-33	0.09920134848590578
2108	34-35	0.028431862565888366
2108	36-37	0.20001641168006046
2108	38-39	0.02310900694173057
2108	40-41	0.142407208977815
2108	42-43	0.036656836062931575
2108	44-45	0.13484967324177433
2108	46-47	-0.0030244310306386524
2108	48-49	0.11056489911666745
2108	50-51	-0.03072202492835885
2108	52-53	-0.13879917507649964
2108	54-55	-0.080438447737059
2108	56-57	0.029859376256538894
2108	58-59	-0.16737805501831815
2108	60-61	-0.23792710100283188
2108	62-63	-0.00679458858360249
2108	64-65	-0.11299580061577075
2108	66-67	-0.09583198894647182
2108	68-69	-0.10782393009692015
2108	70-71	-0.06041575730417037
2108	72-73	-0.11459654058808866
2108	74-75	-0.2050303500592534
2108	76	-0.0656774598857055
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	29.0
14	90.0
15	300.0
16	711.0
17	1176.0
18	1648.0
19	1759.0
20	1822.0
21	1804.0
22	1780.0
23	1908.0
24	2067.0
25	2675.0
26	3522.0
27	4344.0
28	5805.0
29	7877.0
30	10809.0
31	15157.0
32	21575.0
33	31081.0
34	48315.0
35	81661.0
36	173832.0
37	1699395.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.11795981983324	25.890014171584607	26.179527481842364	32.812498526739795
2	26.01046792630022	27.46685046668169	24.65558712130141	21.867094485716684
3	22.987243688081822	27.4289935723425	24.58425775500602	24.99950498456966
4	22.857785366966723	24.231618191298477	26.687790467982875	26.22280597375192
5	24.781981061181078	24.79456859640968	27.993311162927963	22.43013917948128
6	23.774978242893226	25.413526461639137	25.9194322314447	24.89206306402294
7	25.193975332202502	25.686114958611995	26.75586687573604	22.364042833449467
8	23.00784575884923	25.868846368896815	27.712189542822607	23.411118329431353
9	23.03259653036613	24.84402299511679	28.231767167370847	23.89161330714623
10-11	23.71484565418882	25.600312284020056	26.637204605434988	24.04763745635614
12-13	23.723968081405054	25.87745020851936	26.210784170443713	24.187797539631877
14-15	23.981046094893987	25.963205738784602	25.952904703400897	24.102843462920514
16-17	23.89630416765277	25.98701362376753	26.298849772717205	23.817832435862503
18-19	23.810949364164465	25.78936103408252	26.225799638497303	24.17388996325571
20-21	24.038279307506414	25.78137007070706	26.189003491508835	23.991347130277692
22-23	23.83405008424691	25.76574172640639	26.187659878197916	24.212548311148787
24-25	23.784312819579604	25.722533950986872	26.247580317432178	24.245572912001343
26-27	23.709801211232044	25.81345178502564	26.19539154777653	24.281355455965784
28-29	23.7302854211827	25.77887142139202	26.273627557933306	24.217215599491972
30-31	23.72941325113877	25.749076206918335	26.22511604576017	24.296394496182725
32-33	24.0368414055421	25.92110585504251	25.979446959332357	24.06260578008303
34-35	24.06970100125121	25.8694592451439	25.832568809502032	24.22827094410286
36-37	23.90045286840227	25.761050865899847	26.071826267498793	24.266669998199085
38-39	24.203001584992265	25.849399334133526	25.829905155043548	24.11769392583066
40-41	24.29116147591915	25.767415350004196	25.878322378563283	24.063100795513368
42-43	24.169458396546016	25.66838869177322	26.105228022964944	24.056924888715816
44-45	24.211605424614806	25.669873738064236	26.060346623947623	24.058174213373338
46-47	24.022014514795302	25.75461566530545	26.016455255790973	24.20691456410827
48-49	23.858918716580565	25.835468185594014	25.758622933074854	24.546990164750564
50-51	24.355984925130095	25.704784111984747	25.94448944108515	23.99474152180001
52-53	24.291397197552644	25.887916249046505	25.86314190536625	23.957544648034602
54-55	24.14854988765507	25.822550640078525	25.864084791900225	24.164814680366177
56-57	24.10977367894525	25.835020314490375	26.077365725885915	23.977840280678464
58-59	24.33653789036681	25.9985875559721	25.7673210613508	23.89755349231029
60-61	24.235083299310844	25.956086945453073	25.811330290324193	23.997499464911893
62-63	24.33778721502433	25.789997482492954	25.852086560755367	24.020128741727348
64-65	24.37158969726742	26.086511725265492	25.728780574274474	23.813118003192614
66-67	24.23015671717081	26.10329510557029	25.824483557473176	23.84206461978572
68-69	24.479243767284288	26.14848294271116	25.645782987121112	23.72649030288344
70-71	24.412133818228448	26.006201364733972	25.709710694124784	23.871954122912804
72-73	24.482756019623356	26.11416187287438	25.717489508030095	23.685592599472173
74-75	24.67307766650669	25.863212621856295	25.826369330541134	23.63734038109588
76	24.62385898943307	25.938007096164057	25.66419284669702	23.773941067705852
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	6.0
2	3.0
3	2.5
4	3.0
5	5.0
6	7.0
7	6.0
8	5.0
9	6.5
10	10.0
11	20.5
12	29.0
13	36.0
14	47.0
15	70.5
16	126.0
17	162.0
18	187.0
19	281.0
20	429.0
21	508.0
22	688.5
23	1110.0
24	1649.5
25	1948.0
26	2428.5
27	3452.5
28	4594.5
29	5193.0
30	6227.5
31	8681.0
32	12610.5
33	15121.0
34	22263.5
35	34358.0
36	45639.0
37	51968.0
38	58669.5
39	77324.5
40	99392.5
41	108972.0
42	108437.0
43	114080.5
44	125030.5
45	137254.0
46	144171.0
47	150158.5
48	153566.5
49	149907.5
50	148828.0
51	139766.5
52	116783.5
53	96154.5
54	89447.0
55	83708.0
56	73860.0
57	60404.5
58	51058.0
59	44793.5
60	37654.5
61	30873.0
62	24966.0
63	24148.5
64	20646.5
65	15779.5
66	11883.5
67	10170.0
68	7802.5
69	6913.5
70	8311.5
71	8231.0
72	8317.0
73	6401.5
74	3467.0
75	2534.0
76	2190.5
77	1228.0
78	386.5
79	164.0
80	91.0
81	13.5
82	8.5
83	8.0
84	8.0
85	8.5
86	7.5
87	6.0
88	7.0
89	6.0
90	8.0
91	7.5
92	3.0
93	5.5
94	9.0
95	9.0
96	8.0
97	12.0
98	15.0
99	78.5
100	143.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	2121146.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.84649358645149
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.97809676759815	43.088513203173015
2	4.078004691768024	3.820804412768596
3	0.87627230384957	1.2315085458682185
4	0.42509051066411924	0.796559995259522
5	0.30719049557351424	0.7195398790351744
6	0.24851485281500177	0.6985229675121549
7	0.14804569612998864	0.48547952279786805
8	0.11169916379051596	0.41861713280995205
9	0.11563153712062617	0.48752388559016463
>10	1.0731709500688806	11.542641096106491
>50	0.34440203580362533	11.783077166412163
>100	0.28896799163094844	23.453985553657606
>500	0.004507439291817565	1.261199366429593
>1k	4.0556389510869854E-4	0.21202727257934054
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	1.4143298009660816E-4	0.0	0.0
3	1.4143298009660816E-4	0.0	0.0
4	1.8857730679547753E-4	0.0	0.0
5	2.828659601932163E-4	0.0	0.0
6	3.300102868920857E-4	0.0	0.0
7	5.657319203864326E-4	0.0	0.0
8	7.071649004830408E-4	0.0	0.0
9	8.014535538807795E-4	0.0	0.0
10	8.014535538807795E-4	0.0	0.0
11	9.428865339773877E-4	0.0	0.0
12	0.0011786081674717347	0.0	0.0
13	0.0013671854742672122	0.0	0.0
14	0.0018857730679547754	0.0	0.0
15	0.0020272060480513833	4.714432669886938E-5	0.0
16	0.0023572163349434693	4.714432669886938E-5	0.0
17	0.002451504988341208	4.714432669886938E-5	0.0
18	0.0026400822951366857	4.714432669886938E-5	0.0
19	0.002922948255329902	4.714432669886938E-5	4.714432669886938E-5
20	0.0033943915223185956	4.714432669886938E-5	4.714432669886938E-5
21	0.004290133729597114	4.714432669886938E-5	4.714432669886938E-5
22	0.005233020263574501	4.714432669886938E-5	9.428865339773876E-5
23	0.006553061411142844	9.428865339773876E-5	9.428865339773876E-5
24	0.007731669578614579	9.428865339773876E-5	9.428865339773876E-5
25	0.00919314370627953	9.428865339773876E-5	9.428865339773876E-5
26	0.011361782734427521	9.428865339773876E-5	9.428865339773876E-5
27	0.014379019643155163	9.428865339773876E-5	9.428865339773876E-5
28	0.01767912251207602	9.428865339773876E-5	9.428865339773876E-5
29	0.02060207076740592	9.428865339773876E-5	9.428865339773876E-5
30	0.02456219421011095	9.428865339773876E-5	9.428865339773876E-5
31	0.030973822641157184	9.428865339773876E-5	9.428865339773876E-5
32	0.03681971915181699	9.428865339773876E-5	9.428865339773876E-5
33	0.04426852277023835	9.428865339773876E-5	9.428865339773876E-5
34	0.052660212922637104	9.428865339773876E-5	9.428865339773876E-5
35	0.061806212302217764	9.428865339773876E-5	9.428865339773876E-5
36	0.07203653119587242	9.428865339773876E-5	9.428865339773876E-5
37	0.08580267459194228	9.428865339773876E-5	9.428865339773876E-5
38	0.10037027154189292	9.428865339773876E-5	9.428865339773876E-5
39	0.11677649723309946	9.428865339773876E-5	9.428865339773876E-5
40	0.13638853713982912	9.428865339773876E-5	9.428865339773876E-5
41	0.15717918521403054	9.428865339773876E-5	9.428865339773876E-5
42	0.17697980242755568	9.428865339773876E-5	9.428865339773876E-5
43	0.20041053279689375	9.428865339773876E-5	9.428865339773876E-5
44	0.22695278872835722	9.428865339773876E-5	9.428865339773876E-5
45	0.25467365282729243	9.428865339773876E-5	9.428865339773876E-5
46	0.2870146609427168	9.428865339773876E-5	9.428865339773876E-5
47	0.31949710203823783	9.428865339773876E-5	9.428865339773876E-5
48	0.3581554499313107	9.428865339773876E-5	9.428865339773876E-5
49	0.3985109935855429	9.428865339773876E-5	9.428865339773876E-5
50	0.4448538667305315	9.428865339773876E-5	9.428865339773876E-5
51	0.4942611211109466	9.428865339773876E-5	9.428865339773876E-5
52	0.5467799010534872	1.4143298009660816E-4	9.428865339773876E-5
53	0.6053802991401818	1.4143298009660816E-4	9.428865339773876E-5
54	0.6651593053943482	1.4143298009660816E-4	9.428865339773876E-5
55	0.728002692883941	1.4143298009660816E-4	9.428865339773876E-5
56	0.7941461832424548	1.4143298009660816E-4	9.428865339773876E-5
57	0.8671256009723046	1.4143298009660816E-4	9.428865339773876E-5
58	0.944913740025439	1.4143298009660816E-4	9.428865339773876E-5
59	1.025813404640699	1.4143298009660816E-4	9.428865339773876E-5
60	1.1130304090336074	1.4143298009660816E-4	9.428865339773876E-5
61	1.2024160524546637	1.4143298009660816E-4	9.428865339773876E-5
62	1.2962332625854138	1.4143298009660816E-4	9.428865339773876E-5
63	1.3961792351870168	1.4143298009660816E-4	9.428865339773876E-5
64	1.497869547876478	1.4143298009660816E-4	9.428865339773876E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTGTGT	2160	0.0	25.439814	2
CTTTGTG	2775	0.0	19.927927	1
GCCGTAT	180	1.7045386E-7	19.444445	47
GTGTTTG	2920	0.0	18.938356	5
TGTGTTT	3155	0.0	17.749603	4
GTCCGGC	130	0.0022916554	16.153847	51
TAACTTT	1200	0.0	15.458334	1
TTGTGTT	3785	0.0	15.257595	3
CGCCGTA	230	2.2180902E-6	15.217392	46
CGTATCA	280	1.1235479E-6	13.75	49
TGGTCGC	285	1.371307E-6	13.508771	42
GTTTGAC	910	0.0	13.076922	2
CCGTATC	300	3.43645E-5	11.666667	48
GTATCAT	425	7.388917E-8	11.529411	50
ACTTTGT	1690	0.0	10.56213	3
GTGGTCG	365	2.1646934E-5	10.547945	41
TGTTTGA	5840	0.0	10.488013	1
TTGAGGG	570	3.5543053E-9	10.438596	9
TTTGAGT	1125	0.0	9.333333	3
AACTTTG	2215	0.0	9.006771	2
>>END_MODULE