FastQCFastQC Report
Mon 15 Sep 2014
pBOB11_5a_GAGTGG_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamepBOB11_5a_GAGTGG_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121146
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTGT21600.025.4398142
CTTTGTG27750.019.9279271
GCCGTAT1801.7045386E-719.44444547
GTGTTTG29200.018.9383565
TGTGTTT31550.017.7496034
GTCCGGC1300.002291655416.15384751
TAACTTT12000.015.4583341
TTGTGTT37850.015.2575953
CGCCGTA2302.2180902E-615.21739246
CGTATCA2801.1235479E-613.7549
TGGTCGC2851.371307E-613.50877142
GTTTGAC9100.013.0769222
CCGTATC3003.43645E-511.66666748
GTATCAT4257.388917E-811.52941150
ACTTTGT16900.010.562133
GTGGTCG3652.1646934E-510.54794541
TGTTTGA58400.010.4880131
TTGAGGG5703.5543053E-910.4385969
TTTGAGT11250.09.3333333
AACTTTG22150.09.0067712
Produced by FastQC (version 0.11.2)