##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	pBOB11_5a_GAGTGG_L001_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2121146
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.75808548775049	34.0	34.0	34.0	34.0	34.0
2	33.81317504782792	34.0	34.0	34.0	34.0	34.0
3	33.75911229118599	34.0	34.0	34.0	34.0	34.0
4	33.79549262521297	34.0	34.0	34.0	34.0	34.0
5	33.83136097185201	34.0	34.0	34.0	34.0	34.0
6	37.603348850102726	38.0	38.0	38.0	37.0	38.0
7	37.57506838284588	38.0	38.0	38.0	37.0	38.0
8	37.52288385617963	38.0	38.0	38.0	37.0	38.0
9	37.544217606897405	38.0	38.0	38.0	37.0	38.0
10-11	37.54670777023364	38.0	38.0	38.0	37.0	38.0
12-13	37.47883667602325	38.0	38.0	38.0	37.0	38.0
14-15	37.44842976391064	38.0	38.0	38.0	37.0	38.0
16-17	37.5050241708963	38.0	38.0	38.0	37.0	38.0
18-19	37.441442031807334	38.0	38.0	38.0	37.0	38.0
20-21	37.44561147606058	38.0	38.0	38.0	37.0	38.0
22-23	37.25136034954689	38.0	38.0	38.0	37.0	38.0
24-25	37.4138112133724	38.0	38.0	38.0	37.0	38.0
26-27	37.43935165236151	38.0	38.0	38.0	37.0	38.0
28-29	37.50098366637657	38.0	38.0	38.0	37.0	38.0
30-31	37.370702441038944	38.0	38.0	38.0	37.0	38.0
32-33	37.37208848424389	38.0	38.0	38.0	37.0	38.0
34-35	37.42478240536012	38.0	38.0	38.0	37.0	38.0
36-37	37.48479312598001	38.0	38.0	38.0	37.0	38.0
38-39	37.42681361867594	38.0	38.0	38.0	37.0	38.0
40-41	37.4083929159049	38.0	38.0	38.0	37.0	38.0
42-43	37.27525238715298	38.0	38.0	38.0	37.0	38.0
44-45	37.36213513826959	38.0	38.0	38.0	37.0	38.0
46-47	37.350795513368716	38.0	38.0	38.0	37.0	38.0
48-49	37.36368783666943	38.0	38.0	38.0	37.0	38.0
50-51	37.309701218115116	38.0	38.0	38.0	37.0	38.0
52-53	37.42047129240514	38.0	38.0	38.0	37.0	38.0
54-55	37.35216623466749	38.0	38.0	38.0	37.0	38.0
56-57	37.301706247471884	38.0	38.0	38.0	37.0	38.0
58-59	37.40044273237203	38.0	38.0	38.0	37.0	38.0
60-61	37.271571122402705	38.0	38.0	38.0	37.0	38.0
62-63	37.32738128351372	38.0	38.0	38.0	37.0	38.0
64-65	37.33463160951675	38.0	38.0	38.0	37.0	38.0
66-67	37.354326387716824	38.0	38.0	38.0	37.0	38.0
68-69	37.349978030743756	38.0	38.0	38.0	37.0	38.0
70-71	37.38868870883947	38.0	38.0	38.0	37.0	38.0
72-73	37.306978868969885	38.0	38.0	38.0	37.0	38.0
74-75	37.42095169309421	38.0	38.0	38.0	37.0	38.0
76	37.076395967085716	38.0	38.0	38.0	36.0	38.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.028838017857566456
1101	2	0.05507996337325238
1101	3	-0.06067547322746236
1101	4	0.0459705347555186
1101	5	0.0341671900706757
1101	6	0.038892889307007295
1101	7	-0.010037611872839136
1101	8	0.005496373565300416
1101	9	0.037011734264254414
1101	10-11	0.019287479129765472
1101	12-13	0.0752549093021102
1101	14-15	0.0361199570478874
1101	16-17	0.08347772168165335
1101	18-19	0.023391254068805267
1101	20-21	0.024293507639534084
1101	22-23	0.2891852735088065
1101	24-25	0.06561298769629076
1101	26-27	0.0503159520445422
1101	28-29	0.0339168305301385
1101	30-31	0.030601812821309693
1101	32-33	0.0954194606108274
1101	34-35	0.05919882032893753
1101	36-37	0.042181720425155333
1101	38-39	0.10647027738050951
1101	40-41	0.07175675608388588
1101	42-43	0.11528128236014368
1101	44-45	0.09687767108035672
1101	46-47	0.0967779147278165
1101	48-49	0.093423219720151
1101	50-51	0.09991759662446498
1101	52-53	-0.02202485119618558
1101	54-55	0.07369660928229393
1101	56-57	0.041589574982943134
1101	58-59	0.05137952927380951
1101	60-61	0.059233958541767606
1101	62-63	0.055430264443657506
1101	64-65	0.06546514489398447
1101	66-67	0.0294620730592996
1101	68-69	0.15441538613373496
1101	70-71	0.06660643715476766
1101	72-73	0.17022889168542576
1101	74-75	0.09419701936029412
1101	76	0.10499649752403428
1102	1	0.03428672012188372
1102	2	0.05083081239345688
1102	3	0.018286351054072725
1102	4	0.022272095735246467
1102	5	-0.007905577759728999
1102	6	0.01139938643471794
1102	7	0.03267136884146993
1102	8	-0.11899639884033775
1102	9	0.01534969192271518
1102	10-11	0.039387952656731784
1102	12-13	0.06952850244201159
1102	14-15	0.009451481396283157
1102	16-17	0.028741200214867035
1102	18-19	-0.013369369360916039
1102	20-21	-0.12745897413071106
1102	22-23	0.2640448010422034
1102	24-25	-0.0971198730918843
1102	26-27	-0.04061284758472539
1102	28-29	-0.1056182529777061
1102	30-31	-0.08317131248612242
1102	32-33	0.13728942331187
1102	34-35	0.12558335161774892
1102	36-37	0.10360295668103703
1102	38-39	0.10697793451674187
1102	40-41	0.08540416062004397
1102	42-43	0.10355123003949274
1102	44-45	0.08889954433457348
1102	46-47	0.05112405626358196
1102	48-49	0.0273318814443968
1102	50-51	0.0904342324132017
1102	52-53	-0.046587589837386645
1102	54-55	0.07066637542496323
1102	56-57	0.06853670058686845
1102	58-59	0.05103785939171246
1102	60-61	0.052386017839161525
1102	62-63	0.06636032569878125
1102	64-65	0.045851894582504826
1102	66-67	0.05373315276558088
1102	68-69	0.08675161777020435
1102	70-71	0.024859691609478318
1102	72-73	0.1034645226505404
1102	74-75	0.030464540987111377
1102	76	0.10436777094142968
1103	1	0.015891023145329086
1103	2	0.022419935774841804
1103	3	-0.017161173466810453
1103	4	-0.008677282050541635
1103	5	0.06004363786775713
1103	6	0.048682366378521635
1103	7	0.040268618423404234
1103	8	-0.02700070765424556
1103	9	0.05528145845387655
1103	10-11	0.027561619114258917
1103	12-13	0.09331118505326685
1103	14-15	0.13480457569279736
1103	16-17	0.12060141557175541
1103	18-19	0.13529575800826876
1103	20-21	-0.016896014997016096
1103	22-23	0.2934339925122913
1103	24-25	0.02511524539224297
1103	26-27	0.028963935131159246
1103	28-29	0.015404170327578015
1103	30-31	0.0370109860753729
1103	32-33	0.10380752846994312
1103	34-35	0.02900811708821749
1103	36-37	0.02318993302052519
1103	38-39	0.02548040581174149
1103	40-41	-0.004859058995499765
1103	42-43	0.026159738004103872
1103	44-45	0.0436680617772609
1103	46-47	0.04350009246716979
1103	48-49	-0.03941152787476909
1103	50-51	0.05577070580166321
1103	52-53	-0.14121960732661165
1103	54-55	-0.018151832170929083
1103	56-57	-0.06414659638465281
1103	58-59	-0.030388780613442634
1103	60-61	-0.07402331173595655
1103	62-63	0.009647730900624651
1103	64-65	-0.12849959286114654
1103	66-67	0.038702145229216
1103	68-69	-0.14149156723209444
1103	70-71	-0.035907559042932746
1103	72-73	-0.1901651387814809
1103	74-75	0.04591649978188883
1103	76	-0.14917339421863574
2116	1	-0.05002612025697317
2116	2	-0.009428757925142861
2116	3	0.05327608592681088
2116	4	-0.01382357306869153
2116	5	0.009123927090925577
2116	6	-0.07809594128939779
2116	7	0.013950420699458732
2116	8	-0.0677911630291419
2116	9	0.04817044130872006
2116	10-11	0.018518794143425055
2116	12-13	0.045433586997674524
2116	14-15	0.11552845957423585
2116	16-17	0.08911340020507907
2116	18-19	0.08820971703134006
2116	20-21	-0.05309899993118705
2116	22-23	0.34324981762042484
2116	24-25	0.08186884757460433
2116	26-27	0.08115378322821698
2116	28-29	-0.013939127585324229
2116	30-31	0.018337265384595014
2116	32-33	0.01646449200613631
2116	34-35	-0.07599844981248793
2116	36-37	-0.1359888056399683
2116	38-39	-0.08308132182166617
2116	40-41	-0.1542565560535678
2116	42-43	-0.08511469798459359
2116	44-45	-0.0808524014215024
2116	46-47	-0.06332142387059037
2116	48-49	-0.07071733838817806
2116	50-51	-0.038139626642831104
2116	52-53	-0.1940843438445441
2116	54-55	-0.06999815676510934
2116	56-57	-0.08435514473310235
2116	58-59	-0.1908682190708575
2116	60-61	-0.2020893226380167
2116	62-63	-0.06530978678244992
2116	64-65	-0.11606602474432748
2116	66-67	0.007688899676331573
2116	68-69	-0.12390383426712503
2116	70-71	-0.07470535042413218
2116	72-73	0.028091452325725186
2116	74-75	0.012708953781483956
2116	76	-0.36880727313987194
1117	1	0.0067482139424654974
1117	2	0.05545531298250239
1117	3	0.05634443289621771
1117	4	0.004316135617649763
1117	5	-0.028541536671376377
1117	6	-0.04354548643214429
1117	7	-0.026719020002914817
1117	8	-0.005618742963761747
1117	9	0.013936451310279097
1117	10-11	0.02822992540043856
1117	12-13	0.07811224249783777
1117	14-15	0.10814174528218956
1117	16-17	-0.023258297686865603
1117	18-19	0.13258895584687025
1117	20-21	0.02299990132436136
1117	22-23	0.4480360840972679
1117	24-25	0.09469437001246916
1117	26-27	0.09539769546120169
1117	28-29	0.10543639062984056
1117	30-31	0.13104354804517726
1117	32-33	0.03889643352976435
1117	34-35	0.14325404308478795
1117	36-37	0.15811760498772998
1117	38-39	0.1811632714918474
1117	40-41	0.13611770751623453
1117	42-43	0.21615438311992818
1117	44-45	0.19000637079748373
1117	46-47	0.08982974882653139
1117	48-49	0.12210210247575759
1117	50-51	0.22680600444100207
1117	52-53	0.1566477987150492
1117	54-55	0.2494252208202994
1117	56-57	0.24477990263810767
1117	58-59	0.20926807939776637
1117	60-61	0.1849320892032651
1117	62-63	0.22946986402661196
1117	64-65	0.2284060374274901
1117	66-67	0.2995221486084816
1117	68-69	0.2085534922364829
1117	70-71	0.28703892975072876
1117	72-73	0.285895605624475
1117	74-75	0.24332361216765008
1117	76	0.1273050241776872
2117	1	0.05962921247191133
2117	2	0.045074365801724525
2117	3	-0.03513686081473111
2117	4	0.021940115868119392
2117	5	-0.04723439851716904
2117	6	-0.04106684460896304
2117	7	-0.0012065443169930745
2117	8	-0.024242140582231286
2117	9	0.06688551074933002
2117	10-11	0.0294360929308084
2117	12-13	0.12576856156737648
2117	14-15	0.14580189732273396
2117	16-17	0.036725322405942507
2117	18-19	0.14883907209899405
2117	20-21	-0.009312844742609627
2117	22-23	0.3470414922286267
2117	24-25	0.11164852119851787
2117	26-27	0.08742983284039951
2117	28-29	0.08234468585777677
2117	30-31	0.14797826009139214
2117	32-33	0.1841201077038832
2117	34-35	0.11516807405499208
2117	36-37	0.10931399394504382
2117	38-39	0.08059326296860547
2117	40-41	0.07133423051080712
2117	42-43	0.11565469995079525
2117	44-45	0.10416269873343964
2117	46-47	-0.13651890967188507
2117	48-49	0.038207447368520775
2117	50-51	0.16442154692472855
2117	52-53	0.051309428194009854
2117	54-55	0.13416330496246331
2117	56-57	-0.0398309675630415
2117	58-59	0.14932816946269867
2117	60-61	0.048220047321123616
2117	62-63	0.1721016613946773
2117	64-65	0.17069899735243155
2117	66-67	0.127118649984304
2117	68-69	0.09614968807176183
2117	70-71	0.15820913216145271
2117	72-73	0.194388111333744
2117	74-75	0.18377428701676024
2117	76	0.08825633708985947
1116	1	-0.0370340839408172
1116	2	0.011216630764558033
1116	3	-0.04942763374265269
1116	4	0.04586135135958358
1116	5	0.019898041301473768
1116	6	-0.011553617165525054
1116	7	-0.02087674476170065
1116	8	0.09163134958882324
1116	9	0.02253335793832889
1116	10-11	0.07022202075934558
1116	12-13	0.09966251626528333
1116	14-15	0.03371347457620999
1116	16-17	-0.13143565786614886
1116	18-19	-0.0780047214507178
1116	20-21	-0.04205880183300792
1116	22-23	0.2999743233890513
1116	24-25	0.059729087177963436
1116	26-27	-0.017713528934635292
1116	28-29	-0.01398860300105298
1116	30-31	0.08352614872792685
1116	32-33	0.08476024879796284
1116	34-35	0.034424851892687514
1116	36-37	0.053012384289445436
1116	38-39	0.09246586178950622
1116	40-41	-0.020317504222980176
1116	42-43	0.034569041049245186
1116	44-45	0.023125932188541753
1116	46-47	0.10195187945962658
1116	48-49	0.04298006430052226
1116	50-51	0.11167753791889368
1116	52-53	0.06093874930176213
1116	54-55	0.08881802219342205
1116	56-57	-0.03163227105482491
1116	58-59	-0.0015162922191436223
1116	60-61	0.05423729497187679
1116	62-63	0.03855088918714955
1116	64-65	0.06642878584828793
1116	66-67	0.07739626471710181
1116	68-69	0.04820784702025094
1116	70-71	0.12361205083855964
1116	72-73	0.15202006235564625
1116	74-75	0.10222443609089282
1116	76	-0.06367320515592922
2118	1	0.05060736953920042
2118	2	0.02887396985399704
2118	3	0.03404967824288718
2118	4	-0.02750786698908314
2118	5	0.02800031055291896
2118	6	0.008354795932831394
2118	7	0.038372192447674536
2118	8	0.08763399304955044
2118	9	0.08229621337282111
2118	10-11	0.14985630695459662
2118	12-13	0.0621282481840808
2118	14-15	-0.05913486703690296
2118	16-17	-0.11014273632993365
2118	18-19	0.012587190527895586
2118	20-21	0.0034166230709260503
2118	22-23	0.25585878372135795
2118	24-25	0.04500889674839925
2118	26-27	0.03911945071614298
2118	28-29	-0.07411248781085789
2118	30-31	0.08563379337957144
2118	32-33	3.1111260015848075E-4
2118	34-35	0.07785477641576222
2118	36-37	0.013305744543295361
2118	38-39	-0.05373446129731718
2118	40-41	-0.23548651192126613
2118	42-43	-0.033457528811375425
2118	44-45	-0.14470382952351457
2118	46-47	0.010655718562183836
2118	48-49	-0.2360205531492383
2118	50-51	0.025192487593130863
2118	52-53	-0.11331725606577692
2118	54-55	-0.017682411136895837
2118	56-57	-0.06402859279850048
2118	58-59	-0.12846713429460266
2118	60-61	-0.19564801858447112
2118	62-63	-0.06041737965030336
2118	64-65	-0.26062399374674783
2118	66-67	-0.0628211749490859
2118	68-69	-0.1816796398426277
2118	70-71	0.057037932973479144
2118	72-73	-0.08660038997351194
2118	74-75	0.015412819365067776
2118	76	-0.47434577539099365
1119	1	0.047300821172051144
1119	2	0.04371584044583443
1119	3	0.04252190769700093
1119	4	0.026149385871377717
1119	5	0.02210052985908817
1119	6	0.054964395207321104
1119	7	0.031737173321417345
1119	8	0.105044769388833
1119	9	0.03762255427506034
1119	10-11	0.16269169345332557
1119	12-13	0.05521924660953914
1119	14-15	-0.05399150376742767
1119	16-17	0.07854262685766855
1119	18-19	0.05151789470106394
1119	20-21	0.0663415592191896
1119	22-23	0.34674868858324004
1119	24-25	0.17438161518256834
1119	26-27	0.1255844805150943
1119	28-29	0.16625956460016056
1119	30-31	0.20333834242457982
1119	32-33	0.23101186296599963
1119	34-35	0.15971879279915413
1119	36-37	0.14465062413369623
1119	38-39	0.1108234115081359
1119	40-41	0.08945149958934451
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2101	26-27	-0.09551804797094121
2101	28-29	-0.0665902135044334
2101	30-31	0.0032016280428237565
2101	32-33	0.036492317786866124
2101	34-35	0.07618189219382998
2101	36-37	0.1126465505760521
2101	38-39	0.07707775600582067
2101	40-41	0.0370734989243644
2101	42-43	0.08029569559813154
2101	44-45	0.052098405239341616
2101	46-47	-0.01979992191871105
2101	48-49	0.04176327794085921
2101	50-51	0.0777333071829105
2101	52-53	0.13166404093291106
2101	54-55	0.06406354683696946
2101	56-57	0.12590024284360624
2101	58-59	0.04730278942694355
2101	60-61	0.04167345391068977
2101	62-63	0.02833063503290134
2101	64-65	0.08023155237183488
2101	66-67	-0.02850869271332357
2101	68-69	-7.425401148708488E-4
2101	70-71	0.023451725174361115
2101	72-73	0.25444010562330277
2101	74-75	0.14438799153771242
2101	76	0.10208810739368346
2107	1	0.05463930991264476
2107	2	-0.02450821801875236
2107	3	0.025857994157739483
2107	4	-0.026983034438593734
2107	5	-0.005313364861535774
2107	6	-0.0869116875150624
2107	7	-0.2018068940772224
2107	8	-0.20715054187147075
2107	9	-0.13018070184079988
2107	10-11	-0.011467708654585351
2107	12-13	-0.30116652450805503
2107	14-15	0.025454823553033634
2107	16-17	-0.12696563613537393
2107	18-19	0.002215612437609593
2107	20-21	-0.004545426213006465
2107	22-23	0.19571300304248496
2107	24-25	0.07268114859090957
2107	26-27	-0.003483948352624111
2107	28-29	-0.02411348622304388
2107	30-31	0.012042690307808357
2107	32-33	-0.04102918814790257
2107	34-35	0.011521192333482588
2107	36-37	-0.14547722160407517
2107	38-39	-0.10299620046159674
2107	40-41	0.011874382040218734
2107	42-43	-0.07806995688169849
2107	44-45	-0.044796489410998674
2107	46-47	-0.195182101074046
2107	48-49	-0.04854251369389573
2107	50-51	-0.19341179994734148
2107	52-53	-0.013824742232671383
2107	54-55	-0.13400669041241997
2107	56-57	-0.017629549721469573
2107	58-59	-0.019197056497084475
2107	60-61	0.010081357369763566
2107	62-63	-0.1475226577242097
2107	64-65	0.07420297188258473
2107	66-67	-0.2564269485940045
2107	68-69	-0.04397601680385321
2107	70-71	-0.21599389808452685
2107	72-73	0.0656108035132732
2107	74-75	-0.050536680321876304
2107	76	0.16211876385395385
2106	1	0.010935738654232807
2106	2	0.07700784533766836
2106	3	0.016882170042165967
2106	4	0.02361765622490708
2106	5	-0.005762829815580517
2106	6	-0.03153859666810632
2106	7	-0.3126563172954775
2106	8	-0.06060054555795347
2106	9	0.014022853846540784
2106	10-11	0.0510735147578103
2106	12-13	0.14797547953403978
2106	14-15	0.06756908087815816
2106	16-17	0.0020448462564104375
2106	18-19	0.005149744544702628
2106	20-21	-0.02991868607927728
2106	22-23	0.11812515092666587
2106	24-25	0.08860995499245661
2106	26-27	0.07459823536099464
2106	28-29	0.048524345703619076
2106	30-31	0.020420059605967822
2106	32-33	-0.022370154145207266
2106	34-35	-0.001262290992293913
2106	36-37	-0.07011030994368639
2106	38-39	-0.10256047664501722
2106	40-41	-0.04386196725430125
2106	42-43	-0.05317138178109815
2106	44-45	0.03112637270091767
2106	46-47	-0.0656192948263481
2106	48-49	0.04050032325410058
2106	50-51	-0.11079672108186855
2106	52-53	0.05186222014948072
2106	54-55	-0.168136265545769
2106	56-57	0.06884820281135262
2106	58-59	0.08457977332335531
2106	60-61	0.08590585223489455
2106	62-63	-0.07377764945024978
2106	64-65	0.02417889328407341
2106	66-67	-0.1217830822784407
2106	68-69	0.08509242457452615
2106	70-71	-0.0836570955520628
2106	72-73	0.07936173944111147
2106	74-75	0.04008385879917853
2106	76	0.1611883590886194
2105	1	0.020450775370463248
2105	2	-0.060212948862407245
2105	3	-0.02152598836102726
2105	4	-0.06641348071017461
2105	5	-0.049322510902293004
2105	6	-0.1184397120766505
2105	7	-0.06081690328490197
2105	8	-0.0336482104134177
2105	9	-0.1575826305693937
2105	10-11	-0.10169143674863079
2105	12-13	0.004079913932820034
2105	14-15	-0.0436635575470774
2105	16-17	-0.030817074505506525
2105	18-19	-0.07911689145790035
2105	20-21	-0.11812594375761165
2105	22-23	0.24848434352138327
2105	24-25	0.1180707231946343
2105	26-27	0.022924372247160818
2105	28-29	0.00883147968564657
2105	30-31	-0.03901180998776965
2105	32-33	-0.11521294931148418
2105	34-35	-0.07612588924320107
2105	36-37	-0.17100490022678372
2105	38-39	-0.07005383900828832
2105	40-41	-0.017008624849552234
2105	42-43	-0.23543585574259396
2105	44-45	-0.14539772877554213
2105	46-47	-0.27487294113053906
2105	48-49	-0.17432033429302862
2105	50-51	-0.3479475295633421
2105	52-53	-0.1256991566712884
2105	54-55	-0.5514155456480907
2105	56-57	-0.2563955506971851
2105	58-59	-0.1444986482293089
2105	60-61	-0.10269468591541653
2105	62-63	-0.45359995103707007
2105	64-65	-0.6284917589614025
2105	66-67	-0.7523405413004056
2105	68-69	-0.2033183780420842
2105	70-71	-0.7975409082856899
2105	72-73	-0.3565424116566476
2105	74-75	-0.32849618934137226
2105	76	-0.1846307296466847
2104	1	-0.07148487647050672
2104	2	-0.02174171176139339
2104	3	-0.07209636846662448
2104	4	-0.08076408656005896
2104	5	-0.04516161405088326
2104	6	-0.21283166334149684
2104	7	-0.022042366260365043
2104	8	0.05632257255823703
2104	9	0.028062326165979812
2104	10-11	-0.006828666589420607
2104	12-13	0.06535737868286162
2104	14-15	-0.03793976843206792
2104	16-17	-0.03782556411357518
2104	18-19	-0.06510202920368613
2104	20-21	0.052804420962765164
2104	22-23	0.2731448985244711
2104	24-25	0.18917231895395759
2104	26-27	0.06789030081180414
2104	28-29	0.11122279001975954
2104	30-31	-0.13323686588294947
2104	32-33	-0.020795889893676645
2104	34-35	-0.018237614878636066
2104	36-37	-0.15959657605026933
2104	38-39	-0.09918325138463047
2104	40-41	0.09981337485498898
2104	42-43	-0.09436770043268439
2104	44-45	-0.1416807867358827
2104	46-47	-0.01185094222027061
2104	48-49	-0.05547653749524528
2104	50-51	-0.06029415362120005
2104	52-53	-0.1523021144904746
2104	54-55	-0.038499567144477
2104	56-57	-0.10826594891019425
2104	58-59	8.774425271127484E-4
2104	60-61	-0.12669202464812912
2104	62-63	-0.05708861893992889
2104	64-65	-0.5261416883582655
2104	66-67	-0.07439474530615087
2104	68-69	-0.368480021541032
2104	70-71	-0.054645686839826624
2104	72-73	-0.39011934768150525
2104	74-75	-0.11995074271476369
2104	76	-0.3140143875080952
2111	1	0.021632230923572138
2111	2	-0.03497865042859871
2111	3	0.005611116268283922
2111	4	-0.004971648065811962
2111	5	-0.04694156031096952
2111	6	-0.08447296296539974
2111	7	0.00438900518242491
2111	8	0.011261251142919093
2111	9	-0.01661274745708141
2111	10-11	-0.006108636704858839
2111	12-13	0.022421576747845506
2111	14-15	-0.1185160153109095
2111	16-17	-0.11720766210349609
2111	18-19	-0.05314344799830906
2111	20-21	0.039546116900289974
2111	22-23	0.27687470216170595
2111	24-25	0.07461004144523287
2111	26-27	0.04421692880593042
2111	28-29	0.04295064937389981
2111	30-31	-0.09617625848950695
2111	32-33	-0.01882678759658063
2111	34-35	-0.07855731444455216
2111	36-37	-0.0634247151240217
2111	38-39	0.034979543353010456
2111	40-41	-0.020408708308316648
2111	42-43	-0.018433382862525605
2111	44-45	-0.04355913268931744
2111	46-47	0.07236203228066529
2111	48-49	-0.16143640004603554
2111	50-51	0.006144016836557853
2111	52-53	-0.1252113716086214
2111	54-55	0.05967405461162656
2111	56-57	-0.03371201258028833
2111	58-59	0.11066718872452697
2111	60-61	-0.14583582040882703
2111	62-63	0.016402509457194014
2111	64-65	-0.2996152296616117
2111	66-67	0.034567485397900555
2111	68-69	-0.10468089185479101
2111	70-71	0.0582899451358756
2111	72-73	-0.2240534395174194
2111	74-75	0.01630711376678562
2111	76	-0.1216360633537974
2110	1	0.02244576458305403
2110	2	0.025889100735255965
2110	3	0.07423259218251133
2110	4	0.02235864451944991
2110	5	0.04463519514607128
2110	6	0.05176653191240632
2110	7	-0.008462702018334767
2110	8	0.06775373708402554
2110	9	0.0405000389858543
2110	10-11	-0.0878726940388006
2110	12-13	-0.08933056443629539
2110	14-15	-0.14158646244612783
2110	16-17	-0.11325594946194428
2110	18-19	-0.15687983333535982
2110	20-21	-0.08326660511688289
2110	22-23	0.1942088638232775
2110	24-25	-0.07197056385936662
2110	26-27	-0.04527418521128368
2110	28-29	-0.10001796168066335
2110	30-31	-0.3076873815007275
2110	32-33	-0.069941922594694
2110	34-35	0.00256652014357428
2110	36-37	0.006108581723019313
2110	38-39	0.06539162109994834
2110	40-41	-0.05470209310828977
2110	42-43	0.05987180094948741
2110	44-45	0.01965457303278839
2110	46-47	0.14211390640586785
2110	48-49	0.06523408484057569
2110	50-51	0.15076559097500564
2110	52-53	0.002502584938319785
2110	54-55	0.09845256067912089
2110	56-57	-0.045314891394987455
2110	58-59	0.06219359512304834
2110	60-61	-0.0018684650230440525
2110	62-63	0.0925457672875325
2110	64-65	0.082882729096454
2110	66-67	0.1691227468341694
2110	68-69	0.057989399987803836
2110	70-71	0.1526138424916823
2110	72-73	0.007977752412145378
2110	74-75	0.10686963156518914
2110	76	-0.07434049851813995
2109	1	0.028875210759856884
2109	2	-0.0699504377836675
2109	3	0.03167881178223553
2109	4	-0.0027425557520643906
2109	5	0.05366222017934774
2109	6	-0.004598696308114825
2109	7	0.036349638428077924
2109	8	0.071661487891852
2109	9	0.0064618786727805855
2109	10-11	-0.05386583566431824
2109	12-13	0.036966351229587247
2109	14-15	-0.09576121739067389
2109	16-17	-0.06247507727579915
2109	18-19	-0.06820447353100434
2109	20-21	-0.20412691985049491
2109	22-23	-0.00657884409669407
2109	24-25	-0.13021896211133566
2109	26-27	-0.16935884841828397
2109	28-29	-0.1267849289019196
2109	30-31	-0.6934349013391241
2109	32-33	-0.1382968817889676
2109	34-35	-0.06969594106021049
2109	36-37	-0.026371416913562484
2109	38-39	-0.0608736038799762
2109	40-41	-0.1632312011743977
2109	42-43	-0.00596939332226043
2109	44-45	-0.03236503488718512
2109	46-47	0.06869927573360712
2109	48-49	0.04538400635404116
2109	50-51	0.07272435339992
2109	52-53	0.0060300008648610515
2109	54-55	-0.010127177845049573
2109	56-57	-0.003705145118892972
2109	58-59	0.07317794763692831
2109	60-61	-0.06620511365618853
2109	62-63	0.10912873508948451
2109	64-65	0.1679273594720243
2109	66-67	0.1315694861113812
2109	68-69	-0.03996924711908889
2109	70-71	0.09470076217504442
2109	72-73	-0.36440432169379733
2109	74-75	-0.07282287112758468
2109	76	-0.480719867813157
2108	1	0.014194711221271916
2108	2	-0.04387920755598884
2108	3	-0.06983350323805837
2108	4	-0.0180394133220787
2108	5	0.043362109462556475
2108	6	-0.06116512708103983
2108	7	-0.12055729404550419
2108	8	0.03280940407619681
2108	9	-0.05085536631477083
2108	10-11	-0.0043926103691731555
2108	12-13	0.06543434720920516
2108	14-15	-0.030667258129312813
2108	16-17	0.0168421779189174
2108	18-19	0.02270646479035321
2108	20-21	0.031379131992309794
2108	22-23	0.0953678012029755
2108	24-25	0.036853872342234695
2108	26-27	-0.04298743478642564
2108	28-29	-0.038484457222700996
2108	30-31	-0.027595556218372508
2108	32-33	-0.01568139507814692
2108	34-35	-0.13393398325198547
2108	36-37	-0.2504586219709921
2108	38-39	-0.21783372186241934
2108	40-41	-0.3099902333930018
2108	42-43	-0.06756569783411237
2108	44-45	-0.20759849669158825
2108	46-47	-0.26390181732990214
2108	48-49	-0.3235175806588657
2108	50-51	-0.1684017936880906
2108	52-53	-0.13601465176447647
2108	54-55	-0.17719630060273772
2108	56-57	-0.21279643381505764
2108	58-59	-0.0858143702351768
2108	60-61	-0.381611311600615
2108	62-63	-0.14043898379657094
2108	64-65	-0.0698876610497905
2108	66-67	-0.10797142659772874
2108	68-69	-0.4277604122621739
2108	70-71	-0.19039363939673137
2108	72-73	-0.9862887899013444
2108	74-75	-0.5486621126301543
2108	76	-0.8971648766415825
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	10.0
15	10.0
16	43.0
17	59.0
18	138.0
19	252.0
20	323.0
21	389.0
22	495.0
23	626.0
24	811.0
25	995.0
26	1442.0
27	1960.0
28	3017.0
29	4741.0
30	7608.0
31	12501.0
32	19626.0
33	30990.0
34	49488.0
35	82570.0
36	170633.0
37	1732416.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.313231621019957	26.574832661212383	25.90726899515639	32.20466672261127
2	25.781063632583518	26.990409901062918	25.09879093659748	22.129735529756083
3	22.540975491550324	27.25668105825813	25.03689043564187	25.165453014549684
4	22.77617853745098	24.3182223194443	27.0160092704604	25.889589872644315
5	24.63465504024711	24.859627767254118	27.962950216533894	22.542766975964877
6	23.69535147509884	25.463499447939935	25.906137531315622	24.935011545645608
7	25.232633680095574	25.748628335814693	26.616649678994282	22.402088305095454
8	22.950093958643112	25.897981562796712	27.59117005618661	23.560754422373567
9	22.982104956471645	24.81281345084214	28.204517746538897	24.00056384614732
10-11	23.712959881120867	25.579003048352167	26.5969197782708	24.111117292256168
12-13	23.7094476287818	25.969122351785305	26.090141838421303	24.231288181011585
14-15	23.963696982668804	25.999294720872584	25.87907668779047	24.157931608668147
16-17	23.918697722834732	26.025601255170557	26.15249021048056	23.903210811514153
18-19	23.789145113066237	25.77502915876606	26.15543673089924	24.280388997268457
20-21	23.97840601259885	25.838697571972887	26.175708791379755	24.00718762404851
22-23	23.501588292366485	25.781299354217012	26.16686923012372	24.550243123292788
24-25	23.771324557574065	25.670840196761564	26.281147078041776	24.276688167622595
26-27	23.706265386729626	25.78537733847647	26.171135791689963	24.337221483103942
28-29	23.734056967318608	25.772931236227965	26.26912527473356	24.223886521719862
30-31	23.669492811904508	25.815502563237043	26.160104019242432	24.35490060561602
32-33	23.99837163495582	26.030904991924174	25.893337846616877	24.077385526503125
34-35	24.039858642450827	25.871439306865252	25.81333392420889	24.275368126475026
36-37	23.86250168540968	25.88928343452077	25.979564820149108	24.26865005992044
38-39	24.15755445405455	25.973836784455195	25.82226777411833	24.04634098737192
40-41	24.199230038856353	25.8672198896257	25.90507678396489	24.028473287553048
42-43	24.11010368923214	25.747049000870287	26.068549736793223	24.07429757310435
44-45	24.130210744569208	25.78896030730558	25.995075303633037	24.085753644492176
46-47	24.031584813115174	25.94557376059922	25.974402516375584	24.04843890991002
48-49	23.858235123843432	25.943876564838064	25.81752976928509	24.38035854203341
50-51	24.28253406413326	25.836906087558333	25.81422966641617	24.066330181892244
52-53	24.21316118739587	25.958467733951363	25.80859591937566	24.019775159277106
54-55	24.04339446695324	25.933929111904604	25.889189145867377	24.13348727527478
56-57	24.101169839322704	25.97626471728019	25.892654253879744	24.029911189517364
58-59	24.258301880210038	26.02281973989532	25.792071832867702	23.92680654702694
60-61	24.11144730254306	25.977089742997418	25.81788335173534	24.093579602724187
62-63	24.1932427093656	25.8591110654335	25.884121130747246	24.06352509445366
64-65	24.211393275144662	26.189616367755917	25.6901222263814	23.908868130718016
66-67	24.045445245164643	26.273603985769956	25.742947444447484	23.938003324617917
68-69	24.272115167932807	26.28753513430947	25.564694745199056	23.875654952558666
70-71	24.11637388468309	26.2530254871659	25.581124543053612	24.049476085097396
72-73	24.20137510572115	26.342505419240354	25.555643034472876	23.90047644056562
74-75	24.30445617604823	26.107868105260078	25.707919209710223	23.879756508981465
76	24.267259302282824	26.18707057411418	25.512246681746564	24.03342344185643
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	27.0
1	18.5
2	23.0
3	35.5
4	35.0
5	38.5
6	38.5
7	29.0
8	23.0
9	21.5
10	22.0
11	24.5
12	25.0
13	34.5
14	48.5
15	79.5
16	142.5
17	179.0
18	205.5
19	276.0
20	443.5
21	567.0
22	687.5
23	1051.5
24	1650.0
25	2005.0
26	2532.0
27	3619.0
28	4663.5
29	5148.0
30	6226.5
31	8617.0
32	12399.0
33	14869.0
34	21952.5
35	34100.5
36	45457.5
37	51750.0
38	58424.0
39	77294.0
40	99500.0
41	108906.5
42	108303.0
43	114548.5
44	125945.5
45	138065.5
46	145034.0
47	151120.5
48	154593.0
49	150548.5
50	149118.0
51	139688.5
52	116515.5
53	96273.5
54	89775.0
55	84081.5
56	74065.5
57	60217.5
58	50692.0
59	44395.5
60	37232.5
61	30686.0
62	25006.0
63	23899.5
64	20078.5
65	15496.0
66	11783.5
67	9939.0
68	7618.0
69	6858.5
70	8118.0
71	7816.0
72	7872.0
73	5978.0
74	3188.0
75	2348.0
76	2068.0
77	1177.0
78	360.5
79	155.0
80	79.0
81	2.5
82	1.0
83	0.0
84	0.0
85	0.5
86	0.5
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	1.0
95	1.5
96	1.0
97	1.5
98	2.0
99	4.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	2121146.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.49153129396552
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.86557087166342	41.79105492146284
2	4.00212512736737	3.641256009479969
3	0.8912528969391554	1.2163337715583507
4	0.4793497138459812	0.8722541003271149
5	0.2709767566949928	0.6163573803563773
6	0.20983557511087053	0.5727444979046051
7	0.14489926864744113	0.461418272990345
8	0.15494584023429178	0.5638978831910453
9	0.11593793392201487	0.4746774734253942
>10	1.176602259883282	11.932032653675678
>50	0.37714664583456037	12.250723627600014
>100	0.3068494778634082	24.301807189950054
>500	0.004403249296029905	1.2480325056285868
>1k	1.0438269725220877E-4	0.05740971244964237
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	4.714432669886938E-5	0.0	0.0
2	1.8857730679547753E-4	0.0	0.0
3	2.3572163349434692E-4	0.0	0.0
4	2.828659601932163E-4	0.0	0.0
5	3.300102868920857E-4	4.714432669886938E-5	0.0
6	3.7715461359095505E-4	4.714432669886938E-5	0.0
7	5.657319203864326E-4	4.714432669886938E-5	0.0
8	7.071649004830408E-4	4.714432669886938E-5	4.714432669886938E-5
9	8.014535538807795E-4	4.714432669886938E-5	4.714432669886938E-5
10	8.014535538807795E-4	4.714432669886938E-5	4.714432669886938E-5
11	9.428865339773877E-4	4.714432669886938E-5	4.714432669886938E-5
12	0.0011786081674717347	4.714432669886938E-5	4.714432669886938E-5
13	0.0013671854742672122	4.714432669886938E-5	4.714432669886938E-5
14	0.001980061721352514	4.714432669886938E-5	4.714432669886938E-5
15	0.002121494701449122	4.714432669886938E-5	4.714432669886938E-5
16	0.002545793641738947	4.714432669886938E-5	4.714432669886938E-5
17	0.0027815152752332937	4.714432669886938E-5	4.714432669886938E-5
18	0.0029700925820287712	4.714432669886938E-5	4.714432669886938E-5
19	0.0033001028689208568	4.714432669886938E-5	4.714432669886938E-5
20	0.0038186904626084203	4.714432669886938E-5	4.714432669886938E-5
21	0.004620144016489199	4.714432669886938E-5	4.714432669886938E-5
22	0.005515886223767718	4.714432669886938E-5	4.714432669886938E-5
23	0.006788783044637191	4.714432669886938E-5	4.714432669886938E-5
24	0.007967391212108927	4.714432669886938E-5	4.714432669886938E-5
25	0.009476009666472746	4.714432669886938E-5	4.714432669886938E-5
26	0.011691793021319608	4.714432669886938E-5	4.714432669886938E-5
27	0.01461474127664951	4.714432669886938E-5	4.714432669886938E-5
28	0.018056277125666976	4.714432669886938E-5	9.428865339773876E-5
29	0.021073514034394614	4.714432669886938E-5	9.428865339773876E-5
30	0.025457936417389467	4.714432669886938E-5	9.428865339773876E-5
31	0.031916709175134575	4.714432669886938E-5	9.428865339773876E-5
32	0.03771546135909551	4.714432669886938E-5	1.4143298009660816E-4
33	0.04525855363091461	4.714432669886938E-5	1.4143298009660816E-4
34	0.05369738811001223	4.714432669886938E-5	1.4143298009660816E-4
35	0.06279624316289402	4.714432669886938E-5	1.4143298009660816E-4
36	0.07293227340315094	4.714432669886938E-5	1.4143298009660816E-4
37	0.08679270545261854	4.714432669886938E-5	1.4143298009660816E-4
38	0.10154887970936466	4.714432669886938E-5	1.4143298009660816E-4
39	0.11786081674717346	4.714432669886938E-5	1.4143298009660816E-4
40	0.13728427934710766	4.714432669886938E-5	1.4143298009660816E-4
41	0.15807492742130905	4.714432669886938E-5	1.4143298009660816E-4
42	0.1776869673280387	4.714432669886938E-5	1.4143298009660816E-4
43	0.20121198635077453	4.714432669886938E-5	1.4143298009660816E-4
44	0.22780138660893687	4.714432669886938E-5	1.4143298009660816E-4
45	0.25566368368796866	4.714432669886938E-5	1.4143298009660816E-4
46	0.28800469180339305	4.714432669886938E-5	1.4143298009660816E-4
47	0.32067571020570956	4.714432669886938E-5	1.4143298009660816E-4
48	0.3590983364652881	4.714432669886938E-5	1.4143298009660816E-4
49	0.3997367460797135	4.714432669886938E-5	1.4143298009660816E-4
50	0.44574960893781	4.714432669886938E-5	1.4143298009660816E-4
51	0.494496842744441	4.714432669886938E-5	1.4143298009660816E-4
52	0.5469213340335838	4.714432669886938E-5	1.4143298009660816E-4
53	0.6054274434668806	4.714432669886938E-5	1.4143298009660816E-4
54	0.6652535940477459	4.714432669886938E-5	1.4143298009660816E-4
55	0.728002692883941	4.714432669886938E-5	1.4143298009660816E-4
56	0.7941461832424548	4.714432669886938E-5	1.4143298009660816E-4
57	0.866654157705316	4.714432669886938E-5	1.4143298009660816E-4
58	0.9437351318579673	4.714432669886938E-5	1.4143298009660816E-4
59	1.0248233737800228	4.714432669886938E-5	1.4143298009660816E-4
60	1.1114746462525447	9.428865339773876E-5	1.4143298009660816E-4
61	1.200860289673601	9.428865339773876E-5	1.4143298009660816E-4
62	1.2943946338441579	9.428865339773876E-5	1.4143298009660816E-4
63	1.3941520291389655	9.428865339773876E-5	1.4143298009660816E-4
64	1.4957951975017278	9.428865339773876E-5	1.4143298009660816E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGC	140	1.19216566E-8	25.0	44
TTTGTGT	1815	0.0	23.911846	2
TATGCCG	155	8.756415E-7	20.32258	46
GTGTTTG	2380	0.0	18.67647	5
CTTTGTG	2370	0.0	18.607595	1
ATGCCGT	205	6.676946E-7	17.073172	47
TGTGTTT	2645	0.0	16.937618	4
TCGTATG	220	2.4560519E-5	14.318183	43
GTATGCC	245	4.2738557E-6	14.285714	45
TTGTGTT	3385	0.0	13.441654	3
TGTTTGA	5575	0.0	11.049328	1
GTTTGAC	835	0.0	10.479042	2
TTGAGGG	605	8.54925E-10	10.413223	4
CTCGTAT	270	0.002185653	10.37037	42
TAACTTT	1080	0.0	9.722222	1
GCCGTCT	405	6.78943E-5	9.506173	49
ATATGCC	350	0.0018240296	9.0	30
TTTGACG	530	1.5290401E-5	8.584906	3
TTTGAGT	940	4.0017767E-11	8.56383	8
ACTTTGT	1605	0.0	8.504673	2
>>END_MODULE