FastQCFastQC Report
Mon 15 Sep 2014
pBOB11_5a_GAGTGG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamepBOB11_5a_GAGTGG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121146
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1401.19216566E-825.044
TTTGTGT18150.023.9118462
TATGCCG1558.756415E-720.3225846
GTGTTTG23800.018.676475
CTTTGTG23700.018.6075951
ATGCCGT2056.676946E-717.07317247
TGTGTTT26450.016.9376184
TCGTATG2202.4560519E-514.31818343
GTATGCC2454.2738557E-614.28571445
TTGTGTT33850.013.4416543
TGTTTGA55750.011.0493281
GTTTGAC8350.010.4790422
TTGAGGG6058.54925E-1010.4132234
CTCGTAT2700.00218565310.3703742
TAACTTT10800.09.7222221
GCCGTCT4056.78943E-59.50617349
ATATGCC3500.00182402969.030
TTTGACG5301.5290401E-58.5849063
TTTGAGT9404.0017767E-118.563838
ACTTTGT16050.08.5046732
Produced by FastQC (version 0.11.2)