##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename pBOB11_3_CGTACG_L001_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1598361 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.39774869381823 34.0 34.0 34.0 34.0 34.0 2 33.59456092835098 34.0 34.0 34.0 34.0 34.0 3 33.479730799237466 34.0 34.0 34.0 34.0 34.0 4 33.58199743362106 34.0 34.0 34.0 34.0 34.0 5 33.61343401146549 34.0 34.0 34.0 34.0 34.0 6 37.31989081315172 38.0 38.0 38.0 37.0 38.0 7 37.24483017290837 38.0 38.0 38.0 37.0 38.0 8 37.22737792025706 38.0 38.0 38.0 37.0 38.0 9 37.27723837105635 38.0 38.0 38.0 37.0 38.0 10-11 37.37515429868471 38.0 38.0 38.0 37.0 38.0 12-13 37.312509814741475 38.0 38.0 38.0 37.0 38.0 14-15 37.26304164078078 38.0 38.0 38.0 37.0 38.0 16-17 37.33661575826737 38.0 38.0 38.0 37.0 38.0 18-19 37.35617892328454 38.0 38.0 38.0 37.0 38.0 20-21 37.3574039281489 38.0 38.0 38.0 37.0 38.0 22-23 37.39225869500069 38.0 38.0 38.0 37.0 38.0 24-25 37.39903031918321 38.0 38.0 38.0 37.0 38.0 26-27 37.35818347669894 38.0 38.0 38.0 37.0 38.0 28-29 37.33555560977777 38.0 38.0 38.0 37.0 38.0 30-31 37.27692617625179 38.0 38.0 38.0 37.0 38.0 32-33 37.21138403652241 38.0 38.0 38.0 37.0 38.0 34-35 37.31817468018802 38.0 38.0 38.0 37.0 38.0 36-37 37.17211130651962 38.0 38.0 38.0 37.0 38.0 38-39 37.16628502572323 38.0 38.0 38.0 37.0 38.0 40-41 37.30678238520585 38.0 38.0 38.0 37.0 38.0 42-43 37.2382061999761 38.0 38.0 38.0 37.0 38.0 44-45 37.2115836159666 38.0 38.0 38.0 37.0 38.0 46-47 37.17624773126972 38.0 38.0 38.0 36.5 38.0 48-49 37.11671299537464 38.0 38.0 38.0 36.0 38.0 50-51 37.20343339208101 38.0 38.0 38.0 37.0 38.0 52-53 37.179333392143576 38.0 38.0 38.0 37.0 38.0 54-55 37.19112703575725 38.0 38.0 38.0 36.5 38.0 56-57 37.107328382011325 38.0 38.0 38.0 36.0 38.0 58-59 37.25663257549452 38.0 38.0 38.0 37.0 38.0 60-61 37.24710781857165 38.0 38.0 38.0 37.0 38.0 62-63 37.289267881285895 38.0 38.0 38.0 37.0 38.0 64-65 37.239178758740984 38.0 38.0 38.0 37.0 38.0 66-67 37.303276919294206 38.0 38.0 38.0 37.0 38.0 68-69 37.37727553412527 38.0 38.0 38.0 37.0 38.0 70-71 37.39588115575893 38.0 38.0 38.0 37.0 38.0 72-73 37.396389801803224 38.0 38.0 38.0 37.0 38.0 74-75 37.39600972496201 38.0 38.0 38.0 37.0 38.0 76 36.948667416184456 38.0 38.0 38.0 36.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04141802791581739 1101 2 0.11055657062777868 1101 3 0.07209644019312833 1101 4 0.07448837310880663 1101 5 0.03505380351284515 1101 6 0.07804965053080082 1101 7 0.11935724651361568 1101 8 0.26659832834687336 1101 9 0.07848164016840542 1101 10-11 0.13445215738513383 1101 12-13 0.1430175125609594 1101 14-15 0.1744053719895291 1101 16-17 0.00526270663517181 1101 18-19 -0.03912073299093066 1101 20-21 0.013993198618727831 1101 22-23 0.10216454181911416 1101 24-25 0.05745527303871967 1101 26-27 0.11423873828560005 1101 28-29 0.1339932486138835 1101 30-31 0.007435551450619471 1101 32-33 0.12937340036850742 1101 34-35 0.11543146069414689 1101 36-37 0.14017832847306266 1101 38-39 0.1514307803186199 1101 40-41 0.13402322534664535 1101 42-43 0.06254270884399205 1101 44-45 0.05706759352878521 1101 46-47 0.12721512765359932 1101 48-49 0.16218830521934535 1101 50-51 0.07761458209928662 1101 52-53 0.10170148545797986 1101 54-55 0.11505687875219195 1101 56-57 0.1554198908532527 1101 58-59 0.06967513873144782 1101 60-61 0.1925783307676383 1101 62-63 0.12663985300518732 1101 64-65 0.21282262891052284 1101 66-67 0.09452630751495406 1101 68-69 0.10380195107605061 1101 70-71 0.07682061214852354 1101 72-73 0.08863308483260113 1101 74-75 0.11139912459385215 1101 76 -0.05999578937718297 1102 1 0.017045491484857678 1102 2 0.09795767655509735 1102 3 0.10090054649283786 1102 4 0.055179876298716124 1102 5 0.040825734986100315 1102 6 0.16467725725911464 1102 7 0.08861697695261483 1102 8 0.2915472345397845 1102 9 0.19930993072375713 1102 10-11 0.1559514082142286 1102 12-13 0.14807738036259366 1102 14-15 0.1247614367607568 1102 16-17 0.0620020829877177 1102 18-19 0.036115886008673215 1102 20-21 0.1398499036544223 1102 22-23 0.10040809613882828 1102 24-25 0.059686866972278096 1102 26-27 0.06044334889238456 1102 28-29 0.13099716880654455 1102 30-31 0.040945360056340974 1102 32-33 0.1667706857876894 1102 34-35 0.11642084055981172 1102 36-37 0.13423381542536816 1102 38-39 0.02653293499738396 1102 40-41 0.04540708804723437 1102 42-43 -0.04185306022868929 1102 44-45 0.10007778725929484 1102 46-47 0.04170705057243396 1102 48-49 0.11741853694414317 1102 50-51 0.02772944984823056 1102 52-53 0.011915921357754655 1102 54-55 0.0831151801454908 1102 56-57 0.1503126509464181 1102 58-59 0.06017519274553251 1102 60-61 0.21214817009551723 1102 62-63 0.1010696751012432 1102 64-65 0.2453423440499236 1102 66-67 0.10348287404625012 1102 68-69 0.14958947856614913 1102 70-71 0.022984218163671244 1102 72-73 0.07058090942648931 1102 74-75 0.10475769816542169 1102 76 0.09055649361796014 1103 1 0.007964924891908254 1103 2 0.1042817505731719 1103 3 0.11896939340042678 1103 4 0.09278239078620487 1103 5 0.09423832252086584 1103 6 0.05764679001951123 1103 7 -0.019364128551877968 1103 8 0.2889119237041484 1103 9 0.15441646425627198 1103 10-11 0.0818869659604573 1103 12-13 0.151209961921154 1103 14-15 0.004255548918600027 1103 16-17 0.13418479234709224 1103 18-19 0.03914716545712338 1103 20-21 0.10587819343064808 1103 22-23 0.04356260464832218 1103 24-25 0.027102306869835502 1103 26-27 0.026056014422493945 1103 28-29 0.13132353071793545 1103 30-31 0.07593373131328462 1103 32-33 0.12474570980564437 1103 34-35 0.0732602057646119 1103 36-37 0.2266112600317527 1103 38-39 0.09341287928513964 1103 40-41 0.149923034197748 1103 42-43 0.18622441275797286 1103 44-45 0.10079877380856317 1103 46-47 0.06930053578837203 1103 48-49 0.1641441772305967 1103 50-51 0.024146916683967845 1103 52-53 0.0752490598147233 1103 54-55 0.03935479653920737 1103 56-57 0.21761863528597303 1103 58-59 0.16245986052277317 1103 60-61 0.2626110126607202 1103 62-63 0.06480861558757312 1103 64-65 0.22247158672676903 1103 66-67 0.06731807673671142 1103 68-69 0.17635563521672992 1103 70-71 -0.013375455510256984 1103 72-73 0.05936734262678556 1103 74-75 0.10766846786210493 1103 76 0.12055513396332174 2116 1 -0.030013559922018374 2116 2 0.09244509278164514 2116 3 0.001848364831957383 2116 4 0.06607720458734434 2116 5 0.0060173001776675505 2116 6 0.08561190428071797 2116 7 -0.2239848747796671 2116 8 0.139356534523003 2116 9 0.008861718536415708 2116 10-11 -0.050321108927853686 2116 12-13 -0.02538887136452672 2116 14-15 -0.09988571397335733 2116 16-17 -0.0047112858995674856 2116 18-19 -0.173253182056051 2116 20-21 0.0668440023876471 2116 22-23 -0.05876404781592015 2116 24-25 -0.1075410872121978 2116 26-27 -0.18823629109160578 2116 28-29 -0.003685363702075506 2116 30-31 -0.1149507414919384 2116 32-33 -0.1779420632728872 2116 34-35 -0.07110708392463749 2116 36-37 0.01927985047139913 2116 38-39 -0.19702499943011986 2116 40-41 0.07035769560653904 2116 42-43 -0.09871699535349165 2116 44-45 -0.2562244299124927 2116 46-47 -0.11455836438862832 2116 48-49 0.0556702382393226 2116 50-51 -0.07305501832754402 2116 52-53 0.041696657660232006 2116 54-55 -0.1991881217230329 2116 56-57 0.010225358685218566 2116 58-59 -0.07831217182879158 2116 60-61 0.13488741674780869 2116 62-63 -0.14438299209055572 2116 64-65 0.12577389839452735 2116 66-67 -0.08390623508199013 2116 68-69 0.08177139770458552 2116 70-71 -0.173269058524383 2116 72-73 -0.0035135624940707544 2116 74-75 -0.10339947955697681 2116 76 -0.13053569269161613 1117 1 -0.19584064355014874 1117 2 0.041316957838347435 1117 3 0.006568404698825248 1117 4 0.06728898276247719 1117 5 -0.08274122843972975 1117 6 -0.29784496897811863 1117 7 -1.0027988914948907 1117 8 -0.09856424742236669 1117 9 -0.13707824290548132 1117 10-11 -0.17005805921341022 1117 12-13 -0.17375205357701162 1117 14-15 -0.3854993018177666 1117 16-17 -0.11484554013807724 1117 18-19 -0.2906603425431129 1117 20-21 -0.008932738697531306 1117 22-23 -0.20423268761645375 1117 24-25 -0.05292644732553242 1117 26-27 -0.16103196614086102 1117 28-29 -0.004372071559288315 1117 30-31 -0.181317353328609 1117 32-33 -0.07629350019704617 1117 34-35 -0.10283776334482297 1117 36-37 0.006727017647683908 1117 38-39 -0.36181500206441797 1117 40-41 -0.020401335261759357 1117 42-43 -0.3000644595737043 1117 44-45 -0.0821889557859592 1117 46-47 -0.21756406756506408 1117 48-49 -0.0036032821200180365 1117 50-51 -0.1838499450341473 1117 52-53 0.03560364951851369 1117 54-55 -0.2723908549075489 1117 56-57 0.06723922539632099 1117 58-59 -0.29868504558204734 1117 60-61 -0.030770702518331916 1117 62-63 -0.5264262639699311 1117 64-65 0.0018360779508981295 1117 66-67 -0.16733002577871048 1117 68-69 0.07120701422875442 1117 70-71 -0.4688344571287075 1117 72-73 -0.20294774721165965 1117 74-75 -0.32582691185238133 1117 76 -0.01877362538664329 2117 1 0.031835711932018285 2117 2 0.04072727516494723 2117 3 -0.0077315985438062285 2117 4 0.10506359450275227 2117 5 0.009460493147962268 2117 6 -0.31284508575819103 2117 7 0.08245997137559868 2117 8 0.19931609223304037 2117 9 0.04908903286941069 2117 10-11 0.09013582064915227 2117 12-13 0.10615373551727458 2117 14-15 0.06506950783502674 2117 16-17 0.1359455598145516 2117 18-19 -0.06002162811255829 2117 20-21 0.11060657412316743 2117 22-23 0.07153710280753245 2117 24-25 0.12164473738448578 2117 26-27 0.0047074138453879755 2117 28-29 0.16565863593066865 2117 30-31 0.18798896624198136 2117 32-33 0.15167021362692168 2117 34-35 0.11508246747609263 2117 36-37 0.28416528917563966 2117 38-39 -0.0016063036043973966 2117 40-41 0.1985406644983314 2117 42-43 0.025976090548866182 2117 44-45 0.13183966417517468 2117 46-47 0.13518181741132906 2117 48-49 0.26292824090300826 2117 50-51 0.08462143103646014 2117 52-53 0.21541208031407422 2117 54-55 -0.15527268776057923 2117 56-57 0.2746442610890796 2117 58-59 0.15529238162372394 2117 60-61 0.19560843044746434 2117 62-63 -0.10954929869170371 2117 64-65 0.19608436462851841 2117 66-67 0.018599154704091347 2117 68-69 0.20550364332095938 2117 70-71 0.08445002207412244 2117 72-73 0.12047953701220848 2117 74-75 0.031183996766877442 2117 76 0.2426994497773194 1116 1 0.09213341140527831 1116 2 0.009486347456537203 1116 3 0.05774733477227301 1116 4 0.038759019892111723 1116 5 0.03855430719791997 1116 6 0.10906330599298286 1116 7 0.25584653997690765 1116 8 0.31653450671166183 1116 9 0.18352742385073384 1116 10-11 0.15699195711650304 1116 12-13 0.13274524279239586 1116 14-15 0.08480663034122671 1116 16-17 0.2164290174217811 1116 18-19 0.16021495159027666 1116 20-21 0.22396126627612034 1116 22-23 0.10862753172818884 1116 24-25 0.12425220185122754 1116 26-27 0.10883768442143094 1116 28-29 0.215086600401321 1116 30-31 0.1437611472627225 1116 32-33 0.1841612068845606 1116 34-35 0.09093385427453171 1116 36-37 0.29427744250391896 1116 38-39 0.2454088915426169 1116 40-41 0.2969254200359899 1116 42-43 0.141572566176265 1116 44-45 0.19348633879329213 1116 46-47 0.1860511953798749 1116 48-49 0.27595440592192233 1116 50-51 0.04103805855955045 1116 52-53 0.28368129069974657 1116 54-55 0.15131989061676876 1116 56-57 0.29167300121625317 1116 58-59 0.14696997316112004 1116 60-61 0.2204533716651511 1116 62-63 0.15440710744793762 1116 64-65 0.28152618871559554 1116 66-67 0.057489202714016585 1116 68-69 0.20933037914809915 1116 70-71 0.07760579046165361 1116 72-73 0.16971933336889578 1116 74-75 0.15978421121047148 1116 76 0.30036279565519663 2118 1 0.06062313939589359 2118 2 -0.07036136106103186 2118 3 0.057116304993883205 2118 4 0.003716314285277633 2118 5 0.014692382734850185 2118 6 0.1487624114416164 2118 7 0.376658302016466 2118 8 0.3025847491248612 2118 9 0.056504351268976905 2118 10-11 0.14202005349519453 2118 12-13 0.08682532802585285 2118 14-15 0.07472187459863022 2118 16-17 0.10079164401657437 2118 18-19 0.19101076013051 2118 20-21 0.19300247874206633 2118 22-23 0.18929541231246816 2118 24-25 0.17472430766869707 2118 26-27 0.1539090418909126 2118 28-29 0.16059206858541586 2118 30-31 0.17892511501798225 2118 32-33 0.17495867076699056 2118 34-35 0.12678701097878076 2118 36-37 0.23184548928899318 2118 38-39 0.21502408558426822 2118 40-41 0.1845524592176062 2118 42-43 0.20040664978326816 2118 44-45 0.12091291492347978 2118 46-47 0.20252718291925476 2118 48-49 0.25662023121554967 2118 50-51 0.16620936981276202 2118 52-53 0.26901795841813225 2118 54-55 0.21603010574460058 2118 56-57 0.2502557838798154 2118 58-59 0.21879827084720205 2118 60-61 0.161843481384345 2118 62-63 0.19222334446628508 2118 64-65 0.13055048114012635 2118 66-67 0.09717247686979391 2118 68-69 0.1398217269299451 2118 70-71 0.16330291172157985 2118 72-73 0.2062986742538513 2118 74-75 0.22331652260144352 2118 76 0.29341235815602573 1119 1 0.12897407927127347 1119 2 0.0026170609842353088 1119 3 0.10942063934287205 1119 4 0.011756606822018512 1119 5 0.06619323660262211 1119 6 0.19068785111367248 1119 7 0.31745196224228067 1119 8 0.1013289360170262 1119 9 0.05544178367018304 1119 10-11 0.13660641320699796 1119 12-13 -0.11871154770943804 1119 14-15 0.04926551209751295 1119 16-17 0.09015446597971533 1119 18-19 0.18883008250849542 1119 20-21 0.1395771519286697 1119 22-23 0.20821872186048296 1119 24-25 0.05212513173646016 1119 26-27 0.13415431435477387 1119 28-29 0.07419499590399425 1119 30-31 0.1360003274428152 1119 32-33 0.08894027879667732 1119 34-35 0.11042217285889677 1119 36-37 0.0830993290332529 1119 38-39 0.20122420438519129 1119 40-41 0.08580772909613898 1119 42-43 0.18145554130725117 1119 44-45 0.054063948633412906 1119 46-47 0.17021950715574974 1119 48-49 0.02241046577087502 1119 50-51 0.10644577655270382 1119 52-53 0.11188572354618032 1119 54-55 0.18834885895131492 1119 56-57 0.21932802140361218 1119 58-59 0.18060991617566913 1119 60-61 -0.001479350254932399 1119 62-63 0.21096497707210204 1119 64-65 0.09172380432431027 1119 66-67 0.14320608812958824 1119 68-69 0.04720326524888918 1119 70-71 0.11179216332011777 1119 72-73 0.08102187675244465 1119 74-75 0.1376079930588574 1119 76 0.06920688089693527 2119 1 0.08118876389712426 2119 2 0.04546929740072869 2119 3 0.026571340550468392 2119 4 0.019329288446904513 2119 5 -0.06877513752618825 2119 6 -0.013166333526370977 2119 7 0.1023703203179096 2119 8 -0.5524853886679679 2119 9 -0.6338865051037246 2119 10-11 -0.13161908711733616 2119 12-13 -0.3105786426067141 2119 14-15 -0.11248839690822621 2119 16-17 -0.2891469964984239 2119 18-19 -0.031621978524192684 2119 20-21 -0.34464715150496517 2119 22-23 -0.01434736376087642 2119 24-25 0.009848844369116705 2119 26-27 0.13590929903326554 2119 28-29 -0.23244070376379966 2119 30-31 -0.011757686592261507 2119 32-33 -0.14594013041763532 2119 34-35 -0.13441907531464636 2119 36-37 -0.6362342780415062 2119 38-39 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2104 52-53 0.003231034926393761 2104 54-55 -0.06983355389976253 2104 56-57 0.04904308868322005 2104 58-59 -0.14323865546488435 2104 60-61 -0.07907740885431025 2104 62-63 -0.19605459338217202 2104 64-65 -0.07930916119404685 2104 66-67 -0.03210957982155094 2104 68-69 -0.02161889223898328 2104 70-71 -0.01967064803717733 2104 72-73 -0.04588729640996547 2104 74-75 -0.01457217838929381 2104 76 0.06404492939249451 2111 1 0.050661090060032166 2111 2 -0.08957388478019368 2111 3 0.006357291711154289 2111 4 -0.10373150355152916 2111 5 -0.049823746297406046 2111 6 0.004312696071600897 2111 7 -0.04388249494748209 2111 8 -0.18674162658670923 2111 9 0.058164692824952624 2111 10-11 -0.2129372520076558 2111 12-13 -0.18689383705952878 2111 14-15 -0.15914909314329861 2111 16-17 -0.16450721746350183 2111 18-19 -0.28194939167214983 2111 20-21 -0.10324989317444278 2111 22-23 -0.07161540362681507 2111 24-25 -0.07551288398215661 2111 26-27 -0.18895148941838613 2111 28-29 -0.18926561642406625 2111 30-31 -0.06467071040896144 2111 32-33 -0.2078686537111878 2111 34-35 -0.12795682203829273 2111 36-37 -0.03926456763699804 2111 38-39 -0.1440840738085214 2111 40-41 -0.2214010512188267 2111 42-43 -0.1716284545810538 2111 44-45 -0.11438120272357821 2111 46-47 -0.16571285503140132 2111 48-49 -0.19855054677306327 2111 50-51 -0.19372896035801546 2111 52-53 -0.07657027687599083 2111 54-55 -0.2574044293597666 2111 56-57 -0.1428893774104978 2111 58-59 -0.1866808562875235 2111 60-61 0.020358661650000442 2111 62-63 -0.16024344870260165 2111 64-65 -0.03880435756955336 2111 66-67 -0.09341101459131096 2111 68-69 -0.019452466618439246 2111 70-71 -0.11729481123857255 2111 72-73 -0.153046676789792 2111 74-75 -0.11812085880273315 2111 76 -0.29932957732784615 2110 1 -0.025493435298805878 2110 2 -0.12565924887715596 2110 3 -0.061023365543128705 2110 4 -0.1322269571188741 2110 5 -0.019488334628732673 2110 6 -0.04514743172290281 2110 7 -0.02907013199401831 2110 8 0.05929013708770725 2110 9 0.18795720903934665 2110 10-11 0.06878929869667161 2110 12-13 0.11081113920796781 2110 14-15 0.052984826996691936 2110 16-17 -0.07713399384446262 2110 18-19 -0.12574746355078048 2110 20-21 -0.05506197514360878 2110 22-23 0.02122512088907058 2110 24-25 -0.105590986057706 2110 26-27 -0.05671369845291707 2110 28-29 -0.05375515865085134 2110 30-31 0.034183856208329644 2110 32-33 0.07913935700889851 2110 34-35 0.032194891699170114 2110 36-37 0.04449253922062013 2110 38-39 0.09634434277382553 2110 40-41 -0.03034873389863435 2110 42-43 -0.08576051986899813 2110 44-45 -9.144522358610629E-4 2110 46-47 0.062157297322542604 2110 48-49 0.0474536519859825 2110 50-51 0.08191697008236787 2110 52-53 -0.06380838795014654 2110 54-55 0.049674729963669506 2110 56-57 0.039398408308734645 2110 58-59 0.026489638049220332 2110 60-61 -0.05732960672201415 2110 62-63 0.03851826416345716 2110 64-65 0.0478187224451716 2110 66-67 0.01044491693388494 2110 68-69 -0.025635677273079693 2110 70-71 -0.025033149945585365 2110 72-73 -0.010782259005260642 2110 74-75 0.05003423919498573 2110 76 -0.07745579440777561 2109 1 -0.031408829402394645 2109 2 -0.11845264762580854 2109 3 -0.012115484654550812 2109 4 0.033308593095334516 2109 5 0.023483225506680583 2109 6 0.12381663776759666 2109 7 -0.16159904199605535 2109 8 0.14403424798886988 2109 9 0.15823583368296568 2109 10-11 0.049398155731282145 2109 12-13 0.09928759475505444 2109 14-15 0.04484967414778396 2109 16-17 0.06696543600842375 2109 18-19 -0.017022233965711564 2109 20-21 -0.1020735290645689 2109 22-23 -0.04273622485008133 2109 24-25 -0.08526438898729793 2109 26-27 -0.10679089697276112 2109 28-29 -0.04579528018757628 2109 30-31 -0.09177414060950895 2109 32-33 0.05973202602007888 2109 34-35 -0.019378836174190894 2109 36-37 0.126469840270083 2109 38-39 -0.02774373190648305 2109 40-41 0.02812048935453504 2109 42-43 -0.06852758829082006 2109 44-45 0.06388825985791868 2109 46-47 0.03979845962591355 2109 48-49 -0.007107492068470833 2109 50-51 -0.24494491556433928 2109 52-53 -0.12062059855156804 2109 54-55 -0.051947490763623705 2109 56-57 -0.06845609187412549 2109 58-59 -0.12875243836526806 2109 60-61 -0.27153350438171486 2109 62-63 0.055365148360635885 2109 64-65 -0.08266082761652171 2109 66-67 0.038550119474273004 2109 68-69 -0.1292426975833294 2109 70-71 -0.05257412728366262 2109 72-73 -0.020698532685521798 2109 74-75 -0.02908764243292694 2109 76 -0.07364840416695984 2108 1 -0.033873310210339014 2108 2 -0.04690755138466329 2108 3 -0.01062457434905184 2108 4 -0.028232829008402405 2108 5 0.03861133447977494 2108 6 -0.23843205578808835 2108 7 -0.18025933307276176 2108 8 0.027410749906408682 2108 9 0.07800033969367348 2108 10-11 -0.14520473378042453 2108 12-13 0.08054436989480962 2108 14-15 0.020364281121359795 2108 16-17 0.15067428035556674 2108 18-19 0.02587806482365096 2108 20-21 -0.032231896532969984 2108 22-23 -0.08137634612698008 2108 24-25 -0.07301467062056588 2108 26-27 -0.23797601125058065 2108 28-29 -0.016882513622832107 2108 30-31 -0.14863369442954877 2108 32-33 0.08940201408672266 2108 34-35 -0.0804122176862947 2108 36-37 0.16260334961788203 2108 38-39 -0.007407115635217565 2108 40-41 0.016180491662254326 2108 42-43 -0.07818396534841554 2108 44-45 0.030859738227576372 2108 46-47 -0.06084994212430672 2108 48-49 -4.4127769456281385E-6 2108 50-51 -0.07953130182245616 2108 52-53 -0.2218138581794804 2108 54-55 -0.1343358470881455 2108 56-57 -0.12104332227291081 2108 58-59 -0.27222959191080776 2108 60-61 -0.3663664829533033 2108 62-63 -0.08298035871587217 2108 64-65 -0.1898947790754022 2108 66-67 -0.19123870871881365 2108 68-69 -0.19059130338482788 2108 70-71 -0.12373397047998225 2108 72-73 -0.18661259582602696 2108 74-75 -0.2852133556255083 2108 76 -0.11356934948304342 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 0.0 13 8.0 14 41.0 15 165.0 16 361.0 17 678.0 18 958.0 19 1205.0 20 1143.0 21 1134.0 22 1159.0 23 1248.0 24 1511.0 25 1752.0 26 2190.0 27 2933.0 28 3963.0 29 5372.0 30 7510.0 31 10546.0 32 15081.0 33 22567.0 34 35241.0 35 60226.0 36 128383.0 37 1292983.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 14.320553567406591 26.51849395630521 25.167671863660253 33.99328061262795 2 26.044429262225492 27.624547896251222 25.112224334802963 21.218798506720322 3 22.506242331988833 27.91947501221564 24.59569521528616 24.978587440509372 4 22.501800281663527 24.56891778515617 26.803957303763042 26.125324629417257 5 24.600074701522374 25.051099219763245 28.07788728578838 22.270938792926003 6 23.643156958909785 25.607857048564124 26.043678493156424 24.705307499369667 7 24.90388591813739 25.925870313402292 26.92226599622989 22.247977772230428 8 22.562112063545094 26.039236442831125 28.15390265403122 23.244748839592557 9 22.865297639269226 24.801781324744535 28.689701512987366 23.64321952299887 10-11 23.520593908384903 25.78563290770984 26.811746532854592 23.88202665105067 12-13 23.5545662087601 26.09563796914464 26.326999970594876 24.02279585150038 14-15 23.818774356981933 26.148097957845568 26.142467189827578 23.89066049534492 16-17 23.7916841064065 26.217293840377735 26.352025606230384 23.63899644698538 18-19 23.666305671872625 25.950895949037793 26.369011756417983 24.0137866226716 20-21 23.90730254304253 25.947799026627905 26.31689587020704 23.828002560122524 22-23 23.704657458484036 25.880198528367497 26.408614824811167 24.006529188337304 24-25 23.634616960749167 25.840470331796134 26.411430208820157 24.11348249863454 26-27 23.623949783559535 25.89671544788693 26.356561502689317 24.122773265864218 28-29 23.59463850782145 25.884765706870976 26.458228147458552 24.06236763784902 30-31 23.57292876890765 25.934629285874717 26.310389204941814 24.182052740275818 32-33 23.896197417229274 26.072958486849966 26.130298474499813 23.900545621420942 34-35 23.945623047609395 26.01859029343184 25.925307236600492 24.110479422358278 36-37 23.741883091491847 25.914483649188135 26.15388513608628 24.189748123233738 38-39 24.070125584896026 26.021374395396286 25.969070816918084 23.939429202789608 40-41 24.110635832581 25.930155953504872 26.06632669340656 23.89288152050757 42-43 23.99195175557962 25.836716486450804 26.248137936298495 23.923193821671074 44-45 24.08457788947553 25.831117000477366 26.219202045094942 23.865103064952162 46-47 23.906927158507997 25.948455949563332 26.104021557082536 24.04059533484613 48-49 23.79105846551561 25.929405184435804 25.914076982609057 24.36545936743952 50-51 24.18518094473026 25.87244058132049 26.103083095746204 23.839295378203047 52-53 24.12862300819402 26.035576443619433 26.004732347698674 23.831068200487877 54-55 24.058457382280974 25.899405703717747 26.024690292117985 24.01744662188329 56-57 24.02614303026663 25.97642209738601 26.212757943918803 23.784676928428556 58-59 24.19002966163464 26.14362462547572 25.904880061513012 23.76146565137663 60-61 24.091741477676194 26.129578987475295 25.874599042394053 23.904080492454458 62-63 24.21177068259298 25.994722093444473 25.97664107169782 23.816866152264726 64-65 24.275022976661717 26.20637640683175 25.80349495514468 23.715105661361857 66-67 24.14976967030602 26.214134353878755 25.94022877184816 23.695867203967065 68-69 24.416918330715028 26.20271640762006 25.785007266818948 23.59535799484597 70-71 24.235576318491255 26.182508206844386 25.875881606220375 23.70603386844399 72-73 24.434123455214436 26.21053691875615 25.742275993971326 23.61306363205809 74-75 24.553683429463057 26.073177461161777 25.940666720471782 23.432472388903385 76 24.439973197544234 26.11850514370658 25.80987649223173 23.63164516651745 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8.0 1 4.0 2 2.5 3 3.5 4 2.0 5 3.0 6 4.0 7 6.0 8 8.0 9 8.0 10 13.0 11 24.5 12 31.0 13 31.0 14 43.0 15 69.0 16 111.0 17 139.0 18 169.0 19 246.0 20 381.0 21 469.0 22 610.0 23 1000.0 24 1542.0 25 1835.0 26 2242.0 27 3141.0 28 4130.0 29 4627.0 30 5452.5 31 7496.0 32 10683.5 33 12653.0 34 17579.0 35 26489.5 36 35535.5 37 40597.0 38 46137.0 39 60403.5 40 76793.0 41 85551.0 42 86646.0 43 90675.0 44 98065.5 45 105276.0 46 109125.0 47 112580.5 48 114660.5 49 111585.5 50 109886.0 51 102118.5 52 84330.0 53 69559.0 54 64809.0 55 60400.0 56 52986.0 57 43291.0 58 36601.0 59 32244.0 60 27004.0 61 22177.5 62 18234.0 63 17577.5 64 15231.0 65 11793.5 66 8783.0 67 7520.0 68 5688.5 69 4824.5 70 5722.0 71 5652.0 72 5786.0 73 4523.5 74 2481.0 75 1835.0 76 1634.0 77 937.0 78 275.0 79 109.0 80 60.5 81 9.0 82 5.5 83 5.0 84 5.0 85 5.5 86 5.5 87 5.0 88 4.5 89 4.0 90 3.5 91 2.5 92 2.0 93 5.0 94 8.5 95 8.5 96 8.0 97 8.5 98 11.5 99 51.5 100 89.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.25640890887603E-5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 1598361.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.522223639078824 #Duplication Level Percentage of deduplicated Percentage of total 1 92.32739576270423 48.492401282624904 2 3.8793287815381285 4.075019476669215 3 0.764881309703032 1.205198016167225 4 0.3889669395289376 0.8171763434458766 5 0.2900517574308432 0.7617081635345297 6 0.22768171625701145 0.7175010011868027 7 0.19253273021202374 0.7078572979826838 8 0.12461756700799223 0.5236153379001327 9 0.0987318101988359 0.46670527939989087 >10 1.1663220624809163 14.014757895510776 >50 0.3621517511788717 13.430691689176994 >100 0.1763557273472225 14.443326585463396 >500 9.82084411938025E-4 0.3440416309375671 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.876922672662809E-4 0.0 0.0 2 1.876922672662809E-4 0.0 0.0 3 2.502563563550412E-4 0.0 0.0 4 2.502563563550412E-4 6.25640890887603E-5 0.0 5 5.630768017988427E-4 6.25640890887603E-5 0.0 6 6.882049799763633E-4 6.25640890887603E-5 0.0 7 7.507690690651236E-4 6.25640890887603E-5 0.0 8 8.133331581538839E-4 6.25640890887603E-5 0.0 9 8.133331581538839E-4 6.25640890887603E-5 0.0 10 9.384613363314045E-4 6.25640890887603E-5 0.0 11 9.384613363314045E-4 6.25640890887603E-5 0.0 12 9.384613363314045E-4 6.25640890887603E-5 0.0 13 0.0013138458708639663 6.25640890887603E-5 0.0 14 0.0015015381381302472 6.25640890887603E-5 0.0 15 0.0018143585835740487 6.25640890887603E-5 0.0 16 0.00206461493992909 6.25640890887603E-5 0.0 17 0.002314871296284131 6.25640890887603E-5 0.0 18 0.0026902558308166927 6.25640890887603E-5 0.0 19 0.003128204454438015 6.25640890887603E-5 0.0 20 0.0038789735235031384 6.25640890887603E-5 0.0 21 0.0050676912161895845 6.25640890887603E-5 0.0 22 0.005505639839810906 6.25640890887603E-5 0.0 23 0.0066317934434085915 6.25640890887603E-5 0.0 24 0.008070767492450079 6.25640890887603E-5 0.0 25 0.009885126076024127 6.25640890887603E-5 0.0 26 0.013138458708639663 6.25640890887603E-5 0.0 27 0.016391791341255197 6.25640890887603E-5 0.0 28 0.019895380330225775 6.25640890887603E-5 0.0 29 0.023711789764640154 6.25640890887603E-5 0.0 30 0.029342557782628582 6.25640890887603E-5 0.0 31 0.03597435122603717 6.25640890887603E-5 0.0 32 0.04360717009486593 6.25640890887603E-5 0.0 33 0.05199075803275981 6.25640890887603E-5 0.0 34 0.06099998686154129 6.25640890887603E-5 0.0 35 0.0731374201447608 6.25640890887603E-5 0.0 36 0.08521228933889152 6.25640890887603E-5 0.0 37 0.0989763889384188 6.25640890887603E-5 0.0 38 0.1142420266760763 6.25640890887603E-5 0.0 39 0.13100920255186407 6.25640890887603E-5 0.0 40 0.1512799674166224 6.25640890887603E-5 0.0 41 0.17292714224133346 6.25640890887603E-5 0.0 42 0.20064303370765427 6.25640890887603E-5 0.0 43 0.22748302792673245 6.25640890887603E-5 0.0 44 0.2548235348585207 6.25640890887603E-5 0.0 45 0.28591788713563454 6.25640890887603E-5 0.0 46 0.3232060842325357 6.25640890887603E-5 0.0 47 0.3599937686167268 6.25640890887603E-5 0.0 48 0.40109837514804225 6.25640890887603E-5 0.0 49 0.4433291352829555 6.25640890887603E-5 0.0 50 0.49413117562302883 6.25640890887603E-5 0.0 51 0.5479362922393627 6.25640890887603E-5 0.0 52 0.6033055110829155 6.25640890887603E-5 0.0 53 0.6641803697662794 6.25640890887603E-5 0.0 54 0.7296224069531226 6.25640890887603E-5 0.0 55 0.8015711094051969 6.25640890887603E-5 0.0 56 0.8755218627081116 6.25640890887603E-5 0.0 57 0.9531638972672631 6.25640890887603E-5 0.0 58 1.03355875174632 6.25640890887603E-5 0.0 59 1.1224623223414485 6.25640890887603E-5 0.0 60 1.2168089686872992 6.25640890887603E-5 0.0 61 1.3137833067748774 6.25640890887603E-5 0.0 62 1.410757644862456 6.25640890887603E-5 0.0 63 1.5177422372042362 6.25640890887603E-5 0.0 64 1.6336109301966202 6.25640890887603E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGTGT 1080 0.0 22.36111 2 GTGTTTG 1625 0.0 15.076923 5 CTTTGTG 1820 0.0 14.038461 1 TGTGTTT 1865 0.0 12.949062 4 TTGAGGG 485 2.4374458E-10 12.268042 4 TGTTTGA 3480 0.0 11.666666 1 TTGTGTT 2170 0.0 11.612904 3 TAACTTT 815 0.0 11.165645 1 ACTTTGT 1140 0.0 9.210526 3 GTTTGAC 650 3.0444426E-8 9.153846 2 TGTAGGG 1275 9.094947E-12 7.4117646 70 GTTTGAG 2585 0.0 7.040619 2 TAGCCCA 515 0.00749873 6.796117 62 GTAGGGA 1195 4.4534318E-8 6.4435143 68 GAGTGTA 765 2.0170264E-4 6.405229 70 CGTGTAG 1555 1.5279511E-10 6.3022513 68 GTTTGAT 2660 0.0 6.184211 2 TTGATGC 740 9.385783E-4 6.1486487 4 TAGATCT 790 0.0020545332 5.759494 70 AACTTTG 1720 1.8171704E-9 5.697674 2 >>END_MODULE