FastQCFastQC Report
Mon 15 Sep 2014
pBOB11_3_CGTACG_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamepBOB11_3_CGTACG_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1598361
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTGT10800.022.361112
GTGTTTG16250.015.0769235
CTTTGTG18200.014.0384611
TGTGTTT18650.012.9490624
TTGAGGG4852.4374458E-1012.2680424
TGTTTGA34800.011.6666661
TTGTGTT21700.011.6129043
TAACTTT8150.011.1656451
ACTTTGT11400.09.2105263
GTTTGAC6503.0444426E-89.1538462
TGTAGGG12759.094947E-127.411764670
GTTTGAG25850.07.0406192
TAGCCCA5150.007498736.79611762
GTAGGGA11954.4534318E-86.443514368
GAGTGTA7652.0170264E-46.40522970
CGTGTAG15551.5279511E-106.302251368
GTTTGAT26600.06.1842112
TTGATGC7409.385783E-46.14864874
TAGATCT7900.00205453325.75949470
AACTTTG17201.8171704E-95.6976742
Produced by FastQC (version 0.11.2)