##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename pBOB11_3_CGTACG_L001_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1598361 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.76218013327402 34.0 34.0 34.0 34.0 34.0 2 33.81750555725522 34.0 34.0 34.0 34.0 34.0 3 33.76550729153176 34.0 34.0 34.0 34.0 34.0 4 33.80094421723253 34.0 34.0 34.0 34.0 34.0 5 33.836441204458815 34.0 34.0 34.0 34.0 34.0 6 37.60508733634016 38.0 38.0 38.0 37.0 38.0 7 37.577843178105574 38.0 38.0 38.0 37.0 38.0 8 37.531481936809016 38.0 38.0 38.0 37.0 38.0 9 37.54864952285497 38.0 38.0 38.0 37.0 38.0 10-11 37.55016169688825 38.0 38.0 38.0 37.0 38.0 12-13 37.480046435066924 38.0 38.0 38.0 37.0 38.0 14-15 37.447436467731634 38.0 38.0 38.0 37.0 38.0 16-17 37.50803385467989 38.0 38.0 38.0 37.0 38.0 18-19 37.44199214069913 38.0 38.0 38.0 37.0 38.0 20-21 37.45032692864753 38.0 38.0 38.0 37.0 38.0 22-23 37.255682226981264 38.0 38.0 38.0 37.0 38.0 24-25 37.41353674169978 38.0 38.0 38.0 37.0 38.0 26-27 37.4350387678378 38.0 38.0 38.0 37.0 38.0 28-29 37.506087485868335 38.0 38.0 38.0 37.0 38.0 30-31 37.36826098734892 38.0 38.0 38.0 37.0 38.0 32-33 37.36970340242286 38.0 38.0 38.0 37.0 38.0 34-35 37.42496626231495 38.0 38.0 38.0 37.0 38.0 36-37 37.48540723904049 38.0 38.0 38.0 37.0 38.0 38-39 37.42733650283009 38.0 38.0 38.0 37.0 38.0 40-41 37.4091616349498 38.0 38.0 38.0 37.0 38.0 42-43 37.271234721067394 38.0 38.0 38.0 37.0 38.0 44-45 37.35981639942416 38.0 38.0 38.0 37.0 38.0 46-47 37.35086942186402 38.0 38.0 38.0 37.0 38.0 48-49 37.36576843403962 38.0 38.0 38.0 37.0 38.0 50-51 37.30968661022135 38.0 38.0 38.0 37.0 38.0 52-53 37.41793718690583 38.0 38.0 38.0 37.0 38.0 54-55 37.34641923820713 38.0 38.0 38.0 37.0 38.0 56-57 37.299312232968646 38.0 38.0 38.0 37.0 38.0 58-59 37.39790979634763 38.0 38.0 38.0 37.0 38.0 60-61 37.270414505859435 38.0 38.0 38.0 37.0 38.0 62-63 37.32647130404207 38.0 38.0 38.0 37.0 38.0 64-65 37.33523966112787 38.0 38.0 38.0 37.0 38.0 66-67 37.35472086718833 38.0 38.0 38.0 37.0 38.0 68-69 37.3458689870436 38.0 38.0 38.0 37.0 38.0 70-71 37.38268826629279 38.0 38.0 38.0 37.0 38.0 72-73 37.29592063369915 38.0 38.0 38.0 37.0 38.0 74-75 37.41259327523632 38.0 38.0 38.0 37.0 38.0 76 37.06135597652845 38.0 38.0 38.0 36.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.011416767282710794 1101 2 0.035871029254110454 1101 3 -0.014497739247822494 1101 4 0.043024527502048215 1101 5 -0.03447195573416195 1101 6 0.03709770044393679 1101 7 -0.06994683793316625 1101 8 -0.011366072627446044 1101 9 0.06993051109687798 1101 10-11 0.02430112130625872 1101 12-13 0.08561005025593715 1101 14-15 0.038172645449520815 1101 16-17 0.1132555632082628 1101 18-19 0.07239998272328307 1101 20-21 0.029680181305835163 1101 22-23 0.29289617337254015 1101 24-25 0.09255323417080774 1101 26-27 0.0737710136755041 1101 28-29 0.04966330615174286 1101 30-31 0.07325687516806312 1101 32-33 0.13617580574787524 1101 34-35 0.11856249618353587 1101 36-37 0.10131915117150925 1101 38-39 0.12274778407488185 1101 40-41 0.07352949596923253 1101 42-43 0.11552187876466036 1101 44-45 0.12652625602532197 1101 46-47 0.11121619001359306 1101 48-49 0.06354627764163467 1101 50-51 0.1141957671307523 1101 52-53 0.0021573105632768375 1101 54-55 0.11807279259800652 1101 56-57 0.09351496777755841 1101 58-59 0.0848319454075579 1101 60-61 0.0784593775426714 1101 62-63 0.10146817871506641 1101 64-65 0.15621162352395856 1101 66-67 0.0496100092209133 1101 68-69 0.16445294925370746 1101 70-71 0.11520208910156526 1101 72-73 0.19959100489541015 1101 74-75 0.10763099370810636 1101 76 0.23055965051013771 1102 1 0.04994289886635528 1102 2 0.06886924108064818 1102 3 0.022042566673881936 1102 4 0.04224077441051577 1102 5 0.018288053997153497 1102 6 -0.014364105014031736 1102 7 0.0016841765127466601 1102 8 -0.17622143785045807 1102 9 0.003501316091117701 1102 10-11 0.020269332482868663 1102 12-13 0.01814997676165575 1102 14-15 0.03449496722940637 1102 16-17 0.049259856229824095 1102 18-19 0.05676230258851689 1102 20-21 -0.1462125550738378 1102 22-23 0.2604164747623301 1102 24-25 -0.05694017523308759 1102 26-27 -0.0441128507816515 1102 28-29 -0.09923303907112313 1102 30-31 -0.10821418733721089 1102 32-33 0.16704946373949525 1102 34-35 0.12403504473472537 1102 36-37 0.07685362708562593 1102 38-39 0.13838590334851375 1102 40-41 0.06308540142087793 1102 42-43 0.17499626686856118 1102 44-45 0.1256094987496681 1102 46-47 0.07838797982064705 1102 48-49 0.06719832113042656 1102 50-51 0.1055185478107532 1102 52-53 -0.0030159648505616587 1102 54-55 0.11218723394310359 1102 56-57 0.11291184128448606 1102 58-59 0.0693786487042587 1102 60-61 0.06329854732430817 1102 62-63 0.07459677745807625 1102 64-65 0.002932178384703832 1102 66-67 0.056858874486081845 1102 68-69 0.05918477593086635 1102 70-71 0.06361556820558434 1102 72-73 0.132427020781698 1102 74-75 -0.005093318424776783 1102 76 -0.003824022482469047 1103 1 0.05050636035441869 1103 2 0.017848480923944976 1103 3 0.012397978603317483 1103 4 0.018735884917731482 1103 5 0.04682848154693886 1103 6 0.06750054371908476 1103 7 0.07841719298158978 1103 8 0.01992184690041654 1103 9 0.1077382194474481 1103 10-11 0.05090764889443733 1103 12-13 0.10224853581893711 1103 14-15 0.18157191136192807 1103 16-17 0.18455030825115415 1103 18-19 0.1331260059472683 1103 20-21 0.059455143989573855 1103 22-23 0.31180929197793006 1103 24-25 0.04757722689026167 1103 26-27 0.07987034818778938 1103 28-29 0.02358700559811666 1103 30-31 0.11444997864010276 1103 32-33 0.15436726833853243 1103 34-35 0.1223880594608886 1103 36-37 0.06756883230275434 1103 38-39 0.07249142867583203 1103 40-41 0.016819064565659403 1103 42-43 0.17772503996057054 1103 44-45 0.12084667560489493 1103 46-47 0.05511128607064819 1103 48-49 0.009958820990547679 1103 50-51 0.06815257291037113 1103 52-53 -0.15116534497572331 1103 54-55 0.04131742009035122 1103 56-57 -0.00335380566360044 1103 58-59 -0.01565738886342416 1103 60-61 0.02812465152850052 1103 62-63 0.09057143143493107 1103 64-65 -0.14304304230157783 1103 66-67 0.0641655061735662 1103 68-69 -0.09142587027691462 1103 70-71 -0.02316282085678978 1103 72-73 -0.08365698795503818 1103 74-75 0.07413512348941254 1103 76 -0.07891055766806687 2116 1 -0.030365981442521672 2116 2 -0.006792230232996133 2116 3 0.053896494179980436 2116 4 0.023669216708519514 2116 5 0.044172275533547634 2116 6 0.012349134742692058 2116 7 0.06763502942011712 2116 8 -0.04026503232883982 2116 9 0.04101761882481014 2116 10-11 0.032457966410632366 2116 12-13 0.0505023482444642 2116 14-15 0.1279532128615628 2116 16-17 0.12051216443865798 2116 18-19 0.11451336468740436 2116 20-21 0.00822372544940464 2116 22-23 0.3866170661803139 2116 24-25 0.08531780180749848 2116 26-27 0.12465756798876981 2116 28-29 0.06971106030775331 2116 30-31 0.07889535412015647 2116 32-33 0.05698761019937848 2116 34-35 -0.021920586003517428 2116 36-37 -0.048068571749617206 2116 38-39 0.014655579618185754 2116 40-41 -0.13062400315809697 2116 42-43 -0.04135295382848625 2116 44-45 0.009851696035340751 2116 46-47 -0.08416221968607118 2116 48-49 -0.003671760609222474 2116 50-51 -0.012683050825636144 2116 52-53 -0.13156285861484918 2116 54-55 -0.03830281654694545 2116 56-57 0.06308293928167075 2116 58-59 -0.09519890065590175 2116 60-61 -0.13436031555195171 2116 62-63 -0.06254569229308515 2116 64-65 -0.06912977845986035 2116 66-67 0.06509546750528727 2116 68-69 -0.08468668750581543 2116 70-71 -0.04447510727639781 2116 72-73 0.07529513973677382 2116 74-75 0.04176687191367279 2116 76 -0.3045820188926385 1117 1 -0.016434839034261017 1117 2 0.03480667264521031 1117 3 0.0559243461736898 1117 4 0.03761098580621791 1117 5 -0.04659754981400965 1117 6 -0.12058806543785039 1117 7 -0.1482756974398427 1117 8 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0.2508027834487976 1117 72-73 0.23086517493348424 1117 74-75 0.22874716387917005 1117 76 0.0806919623294391 2117 1 0.05478105013034451 2117 2 0.025372770279247447 2117 3 -0.01570673582882165 2117 4 0.0060114321555246875 2117 5 -0.031077591646223368 2117 6 -0.07463442689961397 2117 7 0.0267321653737298 2117 8 -0.08762875373609802 2117 9 0.039113796905247966 2117 10-11 0.02248001820744605 2117 12-13 0.15943503344120558 2117 14-15 0.15807513315374422 2117 16-17 0.028755416079384588 2117 18-19 0.13823922821483592 2117 20-21 -0.04879550871111604 2117 22-23 0.3434884766051738 2117 24-25 0.10211310862082001 2117 26-27 0.12082565541666668 2117 28-29 0.1197265956558482 2117 30-31 0.2095520822694752 2117 32-33 0.20401579607939624 2117 34-35 0.09532401427338044 2117 36-37 0.11779780522246597 2117 38-39 0.09617750069413944 2117 40-41 0.06722884378542204 2117 42-43 0.17780025493468088 2117 44-45 0.12340660915216262 2117 46-47 -0.03882730277359059 2117 48-49 0.09053025154026528 2117 50-51 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54-55 -8.612242489718369E-4 1119 56-57 0.09158238276938135 1119 58-59 -0.017009898481198604 1119 60-61 0.0928138515126804 1119 62-63 -0.03347680203050629 1119 64-65 0.1577597750643207 1119 66-67 0.022738222588522206 1119 68-69 0.1719156518505187 1119 70-71 0.13002482118574932 1119 72-73 0.24123971808774058 1119 74-75 0.03014544527045615 1119 76 0.25370787224625246 2119 1 -0.03017406868458039 2119 2 0.042930196378904384 2119 3 0.029240234913807228 2119 4 0.027043476787369514 2119 5 0.0212811194445095 2119 6 0.06999352232409706 2119 7 0.10503770027463588 2119 8 0.10064258597618192 2119 9 0.03015848663288523 2119 10-11 0.08425615326062541 2119 12-13 -0.004770997022781387 2119 14-15 0.029884695339568168 2119 16-17 0.06432414484653037 2119 18-19 -0.06781377655379828 2119 20-21 0.08164833860380583 2119 22-23 0.29498050885907645 2119 24-25 0.14185917149901428 2119 26-27 0.10438235575906418 2119 28-29 0.11813649712205887 2119 30-31 0.15098330452484987 2119 32-33 0.078154571553263 2119 34-35 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44-45 -0.13053808111708065 2104 46-47 0.014766787385411817 2104 48-49 -0.04992412371005628 2104 50-51 -0.0067753336683651355 2104 52-53 -0.14590270639831715 2104 54-55 -0.023158431609871855 2104 56-57 -0.14290651706192392 2104 58-59 0.03486910355262296 2104 60-61 -0.025897305677574423 2104 62-63 -0.002917165456779003 2104 64-65 -0.45098689277315884 2104 66-67 -0.05183544262213502 2104 68-69 -0.36031164296843343 2104 70-71 -0.07758311059067324 2104 72-73 -0.43074769159686355 2104 74-75 -0.14697268188817958 2104 76 -0.2927718941320947 2111 1 0.055108078542716044 2111 2 -0.038221712681377085 2111 3 0.053190828114608735 2111 4 -0.03988136671047471 2111 5 -0.06747865639830053 2111 6 -0.1477931913966657 2111 7 0.004738883129448368 2111 8 0.013242561148643972 2111 9 0.010084092690803459 2111 10-11 -0.050247473333215 2111 12-13 0.019878314013055842 2111 14-15 -0.12626787874403078 2111 16-17 -0.10288532863234678 2111 18-19 -0.03370251371884336 2111 20-21 -0.0023221609617181116 2111 22-23 0.2253929119873419 2111 24-25 0.05650010324101373 2111 26-27 0.06713705969068684 2111 28-29 0.04450068162613263 2111 30-31 -0.15020730889266787 2111 32-33 -0.01328458324643833 2111 34-35 -0.11311444878800359 2111 36-37 -0.10081260879053389 2111 38-39 0.030528411446802295 2111 40-41 -0.08670458254120916 2111 42-43 0.016535131848151252 2111 44-45 -0.10894204924032636 2111 46-47 0.05178115087696966 2111 48-49 -0.1446449877758269 2111 50-51 0.07405478634327523 2111 52-53 -0.09810063535702795 2111 54-55 0.07171664933424182 2111 56-57 -0.07344571770631347 2111 58-59 0.11347283724816748 2111 60-61 -0.13192961676472237 2111 62-63 0.0540187301856605 2111 64-65 -0.21287340968287083 2111 66-67 0.050114041478977356 2111 68-69 -0.13586132968367792 2111 70-71 0.05519260143552884 2111 72-73 -0.24633671094899512 2111 74-75 0.0764995895335332 2111 76 -0.003496623209024108 2110 1 -0.029300582887209714 2110 2 -0.0015199170494355485 2110 3 0.03429129956358423 2110 4 -0.049828966695535826 2110 5 -0.007942662843156256 2110 6 0.06742255297164235 2110 7 0.019069749254661872 2110 8 0.0773085373289959 2110 9 0.028241591503736174 2110 10-11 -0.1024853220960793 2110 12-13 -0.0739813343297655 2110 14-15 -0.13526171042732216 2110 16-17 -0.12399394062325797 2110 18-19 -0.17583510896452026 2110 20-21 -0.0641394847107577 2110 22-23 0.18724351370276793 2110 24-25 -0.08759727026714614 2110 26-27 -0.06516541990683322 2110 28-29 -0.0966207925099809 2110 30-31 -0.2820936471870894 2110 32-33 -0.07174032110587092 2110 34-35 -0.03156809260305948 2110 36-37 -0.01047337804729409 2110 38-39 0.039458777135415346 2110 40-41 -0.01922633659457773 2110 42-43 0.045724821147089756 2110 44-45 0.022803463458203055 2110 46-47 0.15005074108448468 2110 48-49 0.03778740260028712 2110 50-51 0.07161195686695265 2110 52-53 -0.006959354495435832 2110 54-55 0.1080309800669923 2110 56-57 -0.0380491430471821 2110 58-59 0.03325626593239406 2110 60-61 -0.06865802143190791 2110 62-63 0.08780297959300754 2110 64-65 0.056262532477049376 2110 66-67 0.14082662121716538 2110 68-69 0.0574760968913921 2110 70-71 0.11163243256040545 2110 72-73 -0.020026643886218665 2110 74-75 0.10788424636812266 2110 76 -0.09319308013928662 2109 1 -0.028494852096834222 2109 2 -0.10283278492295267 2109 3 -0.006207629013871951 2109 4 -0.03386905470578938 2109 5 0.001540231976434825 2109 6 0.017388139041635498 2109 7 0.008713687242533297 2109 8 0.029296448518486784 2109 9 -0.030700836982454405 2109 10-11 -0.0647099805260467 2109 12-13 -0.049217438431846006 2109 14-15 -0.1157983102609137 2109 16-17 -0.050431617883482716 2109 18-19 -0.06252590226860377 2109 20-21 -0.2858827830881907 2109 22-23 -0.02097234917968649 2109 24-25 -0.11786260155792405 2109 26-27 -0.21766755694505235 2109 28-29 -0.138736551285092 2109 30-31 -0.7143354019228099 2109 32-33 -0.16072498198129637 2109 34-35 -0.09995627384493844 2109 36-37 -0.06557656067296591 2109 38-39 -0.11617082576881188 2109 40-41 -0.13331648531403317 2109 42-43 0.005332443180684265 2109 44-45 -0.002771833146923086 2109 46-47 0.040523670217211816 2109 48-49 -0.030373625886475963 2109 50-51 -0.022692510326301374 2109 52-53 -0.060309272837670846 2109 54-55 -0.02946020280146655 2109 56-57 -0.01204876702487212 2109 58-59 0.057395950551352826 2109 60-61 -0.12715392342418852 2109 62-63 -0.03112289602292151 2109 64-65 0.06855365219304588 2109 66-67 0.08241264541450732 2109 68-69 -0.06668311196292365 2109 70-71 0.05847940002593788 2109 72-73 -0.3712046389554189 2109 74-75 -0.06375898208581532 2109 76 -0.49503586337053207 2108 1 -0.002872298358774117 2108 2 -0.027438953830035473 2108 3 -0.07662429441533902 2108 4 -0.0595572722633122 2108 5 0.010521751976192206 2108 6 -0.12490573729478882 2108 7 -0.21072530841226467 2108 8 0.009788980302815276 2108 9 -0.04850180501138368 2108 10-11 -0.018008771400822354 2108 12-13 0.04324367188640821 2108 14-15 -0.14974897206111848 2108 16-17 -0.05360777303531705 2108 18-19 -0.008538855880338758 2108 20-21 0.021875567983236976 2108 22-23 0.06218613367484238 2108 24-25 0.04171699058246503 2108 26-27 -0.0426461497807864 2108 28-29 -0.04183003834513954 2108 30-31 -0.057467573702396635 2108 32-33 -0.061918772992719084 2108 34-35 -0.15786512031760935 2108 36-37 -0.2544245667702114 2108 38-39 -0.2379485541194839 2108 40-41 -0.3429584770608898 2108 42-43 -0.15777344788873648 2108 44-45 -0.26558975230982895 2108 46-47 -0.24529765131839554 2108 48-49 -0.2515752965562328 2108 50-51 -0.14124284297238177 2108 52-53 -0.13369390492291444 2108 54-55 -0.2143945693467728 2108 56-57 -0.2692009992910869 2108 58-59 -0.0897390193419767 2108 60-61 -0.3923471268772687 2108 62-63 -0.18755062482907192 2108 64-65 -0.1142553700916693 2108 66-67 -0.18575061230042422 2108 68-69 -0.4207828496112924 2108 70-71 -0.17980909719113214 2108 72-73 -1.0417485707075542 2108 74-75 -0.5908813657811507 2108 76 -0.866950802534717 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 1.0 14 1.0 15 1.0 16 3.0 17 10.0 18 28.0 19 68.0 20 161.0 21 234.0 22 311.0 23 480.0 24 539.0 25 767.0 26 1022.0 27 1468.0 28 2359.0 29 3557.0 30 5669.0 31 9355.0 32 14794.0 33 24006.0 34 37971.0 35 63184.0 36 131420.0 37 1300951.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 14.511427643692507 27.145932614722206 24.813856193938665 33.52878354764662 2 25.850918534673955 27.16182389335075 25.50694117286395 21.48031639911134 3 22.153693689973668 27.67334788574046 25.075311522240597 25.097646902045284 4 22.33731929144918 24.65469315129686 27.07098083599387 25.93700672126009 5 24.36545936743952 25.165654066884763 28.088523180933468 22.380363384742243 6 23.47066776529207 25.684748314054207 26.002949271159643 24.841634649494075 7 24.92509514433848 25.977673379167786 26.809462943602853 22.28776853289088 8 22.518192073004784 26.05012259433257 28.0349057565844 23.396779576078245 9 22.85053251424428 24.831561831150786 28.623321014464192 23.694584640140747 10-11 23.551250312038395 25.79651905921128 26.705794247982777 23.94643638076755 12-13 23.5492795432321 26.140340010798564 26.24923280785755 24.06114763811179 14-15 23.764875394231964 26.275353315052108 26.0204672161045 23.93930407461143 16-17 23.756178985848628 26.225051787424743 26.291588696170642 23.727180530555987 18-19 23.61462773428531 25.99062414560916 26.280639980580105 24.114108139525424 20-21 23.87139075590558 25.982396967893983 26.27059844428136 23.875613831919072 22-23 23.342599074927378 25.947924154806078 26.296062028540483 24.413414741726054 24-25 23.669965671084316 25.827644693532935 26.381618420369367 24.120771215013377 26-27 23.648693880794138 25.885829296385488 26.337010224849077 24.128466597971297 28-29 23.60737029995101 25.944764668307098 26.369762525487044 24.078102506254844 30-31 23.57292876890765 25.99071799174279 26.274414853715776 24.161938385633782 32-33 23.855061528653415 26.138431806081353 26.06682720611927 23.939679459145964 34-35 23.901734339113627 25.979331327528637 25.987965171822886 24.130969161534846 36-37 23.771788726076274 26.02278208740078 26.086816432583127 24.118612753939818 38-39 24.084828145831885 26.12419847581366 25.899343139628655 23.891630238725796 40-41 24.036340976788097 26.00892414166762 26.086284637825873 23.86845024371841 42-43 23.937865100562387 25.964879022949134 26.191016922960458 23.90623895352802 44-45 24.05170046065939 25.966787227666337 26.094230277140145 23.887282034534127 46-47 23.88509229141602 26.092290790378392 26.076274383571672 23.946342534633917 48-49 23.757430267630404 26.089788226814843 25.918112366355285 24.234669139199465 50-51 24.15649530988306 25.94801800093971 25.953523640779526 23.941963048397703 52-53 24.085109684232787 26.150694367542755 25.920114417206126 23.84408153101834 54-55 23.97102406777943 26.02969541924509 26.01220875634478 23.987071756630698 56-57 23.926697410660044 26.112373862975886 26.068328744257396 23.89259998210667 58-59 24.0182286729969 26.22852409436917 25.941104669095406 23.812142563538526 60-61 23.952817917854603 26.141090779867625 25.945171334886176 23.960919967391597 62-63 24.058238407969164 26.02903849630966 25.988246710223784 23.924476385497396 64-65 24.0513563581694 26.361128681192795 25.79417290587045 23.79334205476735 66-67 23.896979468342884 26.387311752476446 25.84312930558241 23.872579473598268 68-69 24.09371224648249 26.397322006730644 25.660661139755035 23.848304607031828 70-71 24.00609123971368 26.31010766654091 25.759543682559823 23.924257411185586 72-73 24.04391123156784 26.432639435021247 25.68678164694959 23.83666768646132 74-75 24.19706812165712 26.219483583495844 25.78403752343807 23.79941077140896 76 24.055829690539245 26.348177914751425 25.623372942658136 23.972619452051195 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 15.0 1 10.5 2 17.0 3 22.5 4 17.0 5 23.0 6 20.0 7 18.0 8 25.0 9 21.0 10 19.5 11 27.5 12 33.0 13 29.0 14 37.5 15 69.5 16 116.5 17 144.0 18 178.0 19 257.0 20 401.0 21 500.0 22 618.5 23 976.0 24 1478.0 25 1741.0 26 2209.0 27 3147.0 28 4059.5 29 4502.0 30 5411.5 31 7598.0 32 10732.0 33 12589.0 34 17528.0 35 26349.5 36 35453.5 37 40675.0 38 46009.0 39 60268.0 40 76721.0 41 84976.5 42 85704.0 43 90360.0 44 98397.0 45 106161.5 46 110545.0 47 113674.5 48 115392.5 49 112300.5 50 110620.0 51 103151.0 52 85110.5 53 69326.5 54 64114.0 55 60177.0 56 52866.0 57 42821.0 58 36150.0 59 31771.0 60 26787.0 61 22093.0 62 18004.0 63 17382.5 64 14816.5 65 11419.0 66 8633.5 67 7301.0 68 5527.5 69 4611.5 70 5529.0 71 5589.0 72 5739.0 73 4398.5 74 2312.0 75 1716.0 76 1539.0 77 909.5 78 280.0 79 103.0 80 54.0 81 3.5 82 1.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 1.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 1598361.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.12896445079056 #Duplication Level Percentage of deduplicated Percentage of total 1 92.03915212390443 47.05866537024015 2 3.8855589281762573 3.973292086203515 3 0.9180874558524947 1.4082258267899685 4 0.4266272980632003 0.872520478256408 5 0.2611841207314039 0.667703681199347 6 0.18438528044449382 0.5656457069457344 7 0.19405559324109095 0.694530306981057 8 0.15040049679459153 0.6151857323193528 9 0.11129172825808122 0.5121207735997031 >10 1.273713683434669 15.080855666938728 >50 0.3647357556457254 13.32935819895685 >100 0.1899179494037105 14.93611913891699 >500 8.895860497977794E-4 0.2857770326523071 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.876922672662809E-4 0.0 0.0 2 2.502563563550412E-4 0.0 6.25640890887603E-5 3 3.128204454438015E-4 0.0 6.25640890887603E-5 4 3.128204454438015E-4 0.0 6.25640890887603E-5 5 6.882049799763633E-4 0.0 6.25640890887603E-5 6 8.758972472426442E-4 0.0 6.25640890887603E-5 7 9.384613363314045E-4 0.0 6.25640890887603E-5 8 0.0010010254254201648 0.0 6.25640890887603E-5 9 0.0010010254254201648 0.0 6.25640890887603E-5 10 0.0011261536035976855 0.0 6.25640890887603E-5 11 0.0011261536035976855 0.0 6.25640890887603E-5 12 0.0011261536035976855 0.0 6.25640890887603E-5 13 0.0015015381381302472 0.0 6.25640890887603E-5 14 0.0015641022272190074 0.0 6.25640890887603E-5 15 0.001876922672662809 0.0 6.25640890887603E-5 16 0.0021897431181066105 6.25640890887603E-5 6.25640890887603E-5 17 0.002439999474461652 6.25640890887603E-5 6.25640890887603E-5 18 0.002752819919905453 6.25640890887603E-5 6.25640890887603E-5 19 0.0031907685435267753 6.25640890887603E-5 6.25640890887603E-5 20 0.003941537612591899 6.25640890887603E-5 6.25640890887603E-5 21 0.005255383483455865 6.25640890887603E-5 6.25640890887603E-5 22 0.005818460285254708 6.25640890887603E-5 6.25640890887603E-5 23 0.007069742067029914 6.25640890887603E-5 6.25640890887603E-5 24 0.008633844294248922 6.25640890887603E-5 6.25640890887603E-5 25 0.01044820287782297 6.25640890887603E-5 6.25640890887603E-5 26 0.013638971421349745 6.25640890887603E-5 6.25640890887603E-5 27 0.016954868143054042 6.25640890887603E-5 6.25640890887603E-5 28 0.020458457132024616 6.25640890887603E-5 6.25640890887603E-5 29 0.024462558833705277 6.25640890887603E-5 6.25640890887603E-5 30 0.030030762762604944 6.25640890887603E-5 6.25640890887603E-5 31 0.036787684384191055 6.25640890887603E-5 6.25640890887603E-5 32 0.044420503253019814 6.25640890887603E-5 6.25640890887603E-5 33 0.05305434754726873 6.25640890887603E-5 6.25640890887603E-5 34 0.06218870455422774 6.25640890887603E-5 6.25640890887603E-5 35 0.07426357374835847 6.25640890887603E-5 6.25640890887603E-5 36 0.08640100703157798 1.251281781775206E-4 6.25640890887603E-5 37 0.10060305525472656 1.251281781775206E-4 6.25640890887603E-5 38 0.11599382117056159 1.251281781775206E-4 6.25640890887603E-5 39 0.1325107406899943 1.251281781775206E-4 6.25640890887603E-5 40 0.1527189414656639 1.251281781775206E-4 6.25640890887603E-5 41 0.1741158599340199 1.251281781775206E-4 6.25640890887603E-5 42 0.2013938027767194 1.251281781775206E-4 6.25640890887603E-5 43 0.2283589251739751 1.251281781775206E-4 6.25640890887603E-5 44 0.2554491757494083 1.251281781775206E-4 6.25640890887603E-5 45 0.28641839984834466 1.251281781775206E-4 6.25640890887603E-5 46 0.32395685330160084 1.251281781775206E-4 6.25640890887603E-5 47 0.36049428132943684 1.251281781775206E-4 6.25640890887603E-5 48 0.4015363237716636 1.251281781775206E-4 6.25640890887603E-5 49 0.44433016070837567 1.251281781775206E-4 6.25640890887603E-5 50 0.4943814319793839 1.251281781775206E-4 6.25640890887603E-5 51 0.5483116767738953 1.876922672662809E-4 6.25640890887603E-5 52 0.6038685878847144 1.876922672662809E-4 6.25640890887603E-5 53 0.6638675493208356 2.502563563550412E-4 6.25640890887603E-5 54 0.7295598428640339 2.502563563550412E-4 6.25640890887603E-5 55 0.8010705966924868 2.502563563550412E-4 6.25640890887603E-5 56 0.8750213499954015 2.502563563550412E-4 6.25640890887603E-5 57 0.9528510768218194 2.502563563550412E-4 6.25640890887603E-5 58 1.0329956749445213 2.502563563550412E-4 6.25640890887603E-5 59 1.121836681450561 2.502563563550412E-4 6.25640890887603E-5 60 1.216308455974589 2.502563563550412E-4 6.25640890887603E-5 61 1.3119063841022147 2.502563563550412E-4 6.25640890887603E-5 62 1.408129953120728 2.502563563550412E-4 6.25640890887603E-5 63 1.5147391609279757 2.502563563550412E-4 6.25640890887603E-5 64 1.6302950334749158 2.502563563550412E-4 6.25640890887603E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCCCTA 50 0.0072111976 28.000002 40 TTTGTGT 1170 0.0 20.341879 2 GTGTTTG 1400 0.0 17.75 5 TGTGTTT 1605 0.0 15.919004 4 CTTTGTG 1655 0.0 14.803625 1 TTGTGTT 2215 0.0 11.534989 3 GTTTGAC 590 4.5474735E-11 11.271186 2 CCGTACG 280 2.3451983E-4 11.250001 70 TGTTTGA 3425 0.0 11.240875 1 TTTGAGT 690 8.009374E-8 8.623189 3 ACGGGCA 1110 1.0280928E-8 6.9369373 2 CGCGCCT 515 0.00749873 6.796117 29 ACGTCTG 1585 5.456968E-12 6.624606 68 CAGTCAC 750 1.5595401E-4 6.533334 64 GTTTGAG 2750 0.0 6.490909 2 GCACACG 2000 0.0 6.2999997 70 CGGGCAG 1335 9.291398E-9 6.292135 3 AACGGGC 1225 7.260314E-8 6.285714 1 AGCCCGC 1230 7.865674E-8 6.2601624 10 GTAGCCG 685 0.0026616906 6.1313868 55 >>END_MODULE