FastQCFastQC Report
Mon 15 Sep 2014
pBOB11_3_CGTACG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamepBOB11_3_CGTACG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1598361
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCTA500.007211197628.00000240
TTTGTGT11700.020.3418792
GTGTTTG14000.017.755
TGTGTTT16050.015.9190044
CTTTGTG16550.014.8036251
TTGTGTT22150.011.5349893
GTTTGAC5904.5474735E-1111.2711862
CCGTACG2802.3451983E-411.25000170
TGTTTGA34250.011.2408751
TTTGAGT6908.009374E-88.6231893
ACGGGCA11101.0280928E-86.93693732
CGCGCCT5150.007498736.79611729
ACGTCTG15855.456968E-126.62460668
CAGTCAC7501.5595401E-46.53333464
GTTTGAG27500.06.4909092
GCACACG20000.06.299999770
CGGGCAG13359.291398E-96.2921353
AACGGGC12257.260314E-86.2857141
AGCCCGC12307.865674E-86.260162410
GTAGCCG6850.00266169066.131386855
Produced by FastQC (version 0.11.2)