##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	pBOB11_1_GTTTCG_L001_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2489360
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.43806159012758	34.0	34.0	34.0	34.0	34.0
2	33.62456173474307	34.0	34.0	34.0	34.0	34.0
3	33.585364511360346	34.0	34.0	34.0	34.0	34.0
4	33.625180367644695	34.0	34.0	34.0	34.0	34.0
5	33.64693616029823	34.0	34.0	34.0	34.0	34.0
6	37.38547457981168	38.0	38.0	38.0	37.0	38.0
7	37.27372698203554	38.0	38.0	38.0	37.0	38.0
8	37.24873059742263	38.0	38.0	38.0	37.0	38.0
9	37.31775596940579	38.0	38.0	38.0	37.0	38.0
10-11	37.393901645402835	38.0	38.0	38.0	37.0	38.0
12-13	37.346928929524054	38.0	38.0	38.0	37.0	38.0
14-15	37.306250401709676	38.0	38.0	38.0	37.0	38.0
16-17	37.37781056175081	38.0	38.0	38.0	37.0	38.0
18-19	37.39445479962721	38.0	38.0	38.0	37.0	38.0
20-21	37.389091774592664	38.0	38.0	38.0	37.0	38.0
22-23	37.42780272841212	38.0	38.0	38.0	37.0	38.0
24-25	37.433443535687886	38.0	38.0	38.0	37.0	38.0
26-27	37.39457491082045	38.0	38.0	38.0	37.0	38.0
28-29	37.37482144004885	38.0	38.0	38.0	37.0	38.0
30-31	37.31298506443423	38.0	38.0	38.0	37.0	38.0
32-33	37.2490270591638	38.0	38.0	38.0	37.0	38.0
34-35	37.35319459620143	38.0	38.0	38.0	37.0	38.0
36-37	37.22075192017225	38.0	38.0	38.0	37.0	38.0
38-39	37.21209286724299	38.0	38.0	38.0	37.0	38.0
40-41	37.343989418967126	38.0	38.0	38.0	37.0	38.0
42-43	37.274626610855805	38.0	38.0	38.0	37.0	38.0
44-45	37.25139192402867	38.0	38.0	38.0	37.0	38.0
46-47	37.22467843140406	38.0	38.0	38.0	37.0	38.0
48-49	37.17065892438217	38.0	38.0	38.0	37.0	38.0
50-51	37.25092513738471	38.0	38.0	38.0	37.0	38.0
52-53	37.21883395732236	38.0	38.0	38.0	37.0	38.0
54-55	37.23196464151428	38.0	38.0	38.0	37.0	38.0
56-57	37.15418019089244	38.0	38.0	38.0	37.0	38.0
58-59	37.29325569624321	38.0	38.0	38.0	37.0	38.0
60-61	37.28075569624321	38.0	38.0	38.0	37.0	38.0
62-63	37.32128277950959	38.0	38.0	38.0	37.0	38.0
64-65	37.273740841019375	38.0	38.0	38.0	37.0	38.0
66-67	37.33875313333547	38.0	38.0	38.0	37.0	38.0
68-69	37.408295706526985	38.0	38.0	38.0	37.0	38.0
70-71	37.42643109072211	38.0	38.0	38.0	37.0	38.0
72-73	37.42944371244015	38.0	38.0	38.0	37.0	38.0
74-75	37.42679825336633	38.0	38.0	38.0	37.0	38.0
76	36.992693302696274	38.0	38.0	38.0	36.0	38.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.006671519225498912
1101	2	0.06880032167118344
1101	3	0.0997974627601863
1101	4	0.03741797070427566
1101	5	0.004565325344472626
1101	6	0.1028230845922451
1101	7	0.06915775182593364
1101	8	0.20690972920817075
1101	9	0.06475030730930342
1101	10-11	0.12693479243769445
1101	12-13	0.10557557368337456
1101	14-15	0.14101241236570417
1101	16-17	0.0034009000332702044
1101	18-19	-0.037460262010171164
1101	20-21	0.024389750257022058
1101	22-23	0.0283694518072366
1101	24-25	0.03442517749385843
1101	26-27	0.04969410295920085
1101	28-29	0.11966224107894874
1101	30-31	0.006182316903931451
1101	32-33	0.027258233714199775
1101	34-35	0.029977077725234835
1101	36-37	0.03981124578695727
1101	38-39	0.08759329357118872
1101	40-41	0.08339714270155696
1101	42-43	-0.009638879920011334
1101	44-45	0.02426385584469415
1101	46-47	0.08450566667497839
1101	48-49	0.05209031822055721
1101	50-51	-8.564617636679372E-4
1101	52-53	-0.007016560202465882
1101	54-55	0.07578684131061664
1101	56-57	0.09500137380558016
1101	58-59	0.016396924875955676
1101	60-61	0.14341710181363965
1101	62-63	0.07310784621883215
1101	64-65	0.1951028644853139
1101	66-67	0.06232442345262257
1101	68-69	0.0608226203029929
1101	70-71	0.06453644447125129
1101	72-73	0.05516811031485247
1101	74-75	0.07370982359332601
1101	76	-0.11525341823668356
1102	1	0.09879733935254364
1102	2	0.08107012495146648
1102	3	0.06642387940745209
1102	4	0.03926852400667258
1102	5	0.11851019725736478
1102	6	0.16136412066023098
1102	7	0.025258156672542498
1102	8	0.25992978924026744
1102	9	0.1655140649644764
1102	10-11	0.17014275959512304
1102	12-13	0.21434574607258128
1102	14-15	0.20581347709604358
1102	16-17	0.08636525981430054
1102	18-19	0.0764286118376063
1102	20-21	0.13089200100922938
1102	22-23	0.11907738286598146
1102	24-25	0.13297175470801648
1102	26-27	0.1151802076778452
1102	28-29	0.21302295074274014
1102	30-31	0.0769624046958981
1102	32-33	0.18906069456067343
1102	34-35	0.12879229674489778
1102	36-37	0.1482536873567497
1102	38-39	0.11605572026832789
1102	40-41	0.09019134146444685
1102	42-43	0.08699746837441324
1102	44-45	0.09655848598671923
1102	46-47	0.10299479736480777
1102	48-49	0.1453172597425194
1102	50-51	0.10697616042582325
1102	52-53	0.11291117025597686
1102	54-55	0.1701161009087997
1102	56-57	0.20794836561299235
1102	58-59	0.13438261445987365
1102	60-61	0.2842549429686585
1102	62-63	0.1715558966198003
1102	64-65	0.30053560182956573
1102	66-67	0.15091006845467092
1102	68-69	0.1720068913895787
1102	70-71	0.10794904597620558
1102	72-73	0.12898669102335703
1102	74-75	0.13987027406865593
1102	76	0.20311358613306396
1103	1	0.009306555760211666
1103	2	0.13251537767592225
1103	3	0.05795306353559937
1103	4	0.08964050594130413
1103	5	0.026335073883551274
1103	6	-0.01786210110100228
1103	7	-0.05670484746239879
1103	8	0.26809420548793383
1103	9	0.0705301814684276
1103	10-11	0.033612424288115506
1103	12-13	0.11662484764143954
1103	14-15	-0.035270526401681934
1103	16-17	0.058778479533643235
1103	18-19	-0.04595658423490079
1103	20-21	0.09089894296655388
1103	22-23	-0.01656218419773836
1103	24-25	-0.01797128170644413
1103	26-27	-0.06140609352635806
1103	28-29	0.07168033898536663
1103	30-31	0.04067497975508161
1103	32-33	0.07582934714651657
1103	34-35	0.03224682169175708
1103	36-37	0.1133128509729815
1103	38-39	0.006081918308296963
1103	40-41	0.10011409620056355
1103	42-43	0.10396555397561258
1103	44-45	0.02632983163340441
1103	46-47	0.01994753391462467
1103	48-49	0.06839979112667294
1103	50-51	0.023056326660700677
1103	52-53	0.005649208461157684
1103	54-55	0.019000271385593237
1103	56-57	0.15007749997300834
1103	58-59	0.08591827542257846
1103	60-61	0.1979882741314185
1103	62-63	0.015413659751381203
1103	64-65	0.20236149940023296
1103	66-67	0.03385529559737677
1103	68-69	0.09840725976680886
1103	70-71	-0.034603582665120314
1103	72-73	0.01704315227630815
1103	74-75	0.05673289308194285
1103	76	0.029662793796426
2116	1	-0.05534571816140499
2116	2	0.08297711822122977
2116	3	0.014041137682589522
2116	4	-0.00279647541665895
2116	5	0.007887566698592252
2116	6	-0.006698880898625248
2116	7	-0.2266894821641543
2116	8	0.16219412866144012
2116	9	0.06243579108962649
2116	10-11	-0.03846596218085807
2116	12-13	-0.014586270728827344
2116	14-15	-0.10709762909585407
2116	16-17	0.013078326294447606
2116	18-19	-0.14667611420941284
2116	20-21	0.017751065993770965
2116	22-23	-0.04436867293284763
2116	24-25	-0.07746420387629627
2116	26-27	-0.12141689337243378
2116	28-29	0.008240771917122913
2116	30-31	-0.07945154947599065
2116	32-33	-0.1440499097468333
2116	34-35	-0.06385480858385506
2116	36-37	0.09355377627473871
2116	38-39	-0.14176734223098464
2116	40-41	0.06889152971760382
2116	42-43	-0.08954236614138722
2116	44-45	-0.25918844373829586
2116	46-47	-0.12047690141526601
2116	48-49	0.005447492975527268
2116	50-51	-0.07067756724702434
2116	52-53	-2.9752287279194434E-4
2116	54-55	-0.14264976946439845
2116	56-57	0.054519953896644324
2116	58-59	-0.00154076539109127
2116	60-61	0.1099845169435909
2116	62-63	-0.05953084678668574
2116	64-65	0.1390694129885901
2116	66-67	-0.05420857567618498
2116	68-69	0.05739680980081374
2116	70-71	-0.15405465025910559
2116	72-73	-0.04571381532553431
2116	74-75	-0.03324296243459912
2116	76	-0.08086257342822734
1117	1	-0.11058638990086678
1117	2	0.027981534170024247
1117	3	-0.007807480887727536
1117	4	0.07323287677252921
1117	5	-0.15243612478617763
1117	6	-0.2855802625058814
1117	7	-1.0958681607727883
1117	8	-0.09344916774979595
1117	9	-0.2150771018338986
1117	10-11	-0.15858661248969952
1117	12-13	-0.0992188563365417
1117	14-15	-0.3368050010666863
1117	16-17	-0.0747744194099198
1117	18-19	-0.227888904239812
1117	20-21	-0.003709828684172578
1117	22-23	-0.16219313567900429
1117	24-25	-0.06596684254255791
1117	26-27	-0.15276932472971083
1117	28-29	-0.0171089892609686
1117	30-31	-0.1579415659537915
1117	32-33	-0.06856538599350159
1117	34-35	-0.14859896602000333
1117	36-37	0.028776881748051153
1117	38-39	-0.3894794699380597
1117	40-41	-0.00347015594836364
1117	42-43	-0.3202473174601437
1117	44-45	-0.12507144625961075
1117	46-47	-0.24026210017068195
1117	48-49	0.04889340885085147
1117	50-51	-0.19169484055804276
1117	52-53	0.0016890445277297772
1117	54-55	-0.29613025846022367
1117	56-57	0.032901092095372064
1117	58-59	-0.3295696121774938
1117	60-61	-0.045238682378418105
1117	62-63	-0.5101764084476201
1117	64-65	-0.07500181301293907
1117	66-67	-0.2698198402421639
1117	68-69	0.011852823702930948
1117	70-71	-0.4927135860941121
1117	72-73	-0.19403561677133752
1117	74-75	-0.31446677738559714
1117	76	-0.006128241121615474
2117	1	0.0077395360025747095
2117	2	0.032596754140087114
2117	3	-0.04858364285715311
2117	4	0.03672401804929848
2117	5	-0.05536229989272812
2117	6	-0.40481302541675745
2117	7	0.005032656102564204
2117	8	0.10947784897950896
2117	9	-0.0019420520592703383
2117	10-11	0.06623032191647837
2117	12-13	0.0017558554755083833
2117	14-15	-0.015691064157401513
2117	16-17	0.06858226366562548
2117	18-19	-0.16387687206995594
2117	20-21	0.05536541924156069
2117	22-23	0.016821447343602358
2117	24-25	0.022912934132158114
2117	26-27	-0.059662187396327226
2117	28-29	0.11065545975737479
2117	30-31	0.13652769869649717
2117	32-33	0.11349671068007439
2117	34-35	0.05571468166540683
2117	36-37	0.20432640506075472
2117	38-39	-0.05213893157820593
2117	40-41	0.09779033967360817
2117	42-43	-0.05223855685724743
2117	44-45	0.09440566778749826
2117	46-47	0.019308139202927066
2117	48-49	0.14566561776883447
2117	50-51	0.0629462489635273
2117	52-53	0.1627916880555702
2117	54-55	-0.2147419244827944
2117	56-57	0.18859027327417266
2117	58-59	0.08809476835666175
2117	60-61	0.10516948855507025
2117	62-63	-0.20412285061165392
2117	64-65	0.14728059519993764
2117	66-67	-0.07346629954678008
2117	68-69	0.08870511003549808
2117	70-71	-0.06267234979905112
2117	72-73	0.03699846071747004
2117	74-75	-0.05685337987370076
2117	76	0.15529541479651954
1116	1	0.07826197980677563
1116	2	0.04948468585069321
1116	3	0.09351585918014393
1116	4	0.004530076271429095
1116	5	0.09270112369555505
1116	6	0.11549846324482616
1116	7	0.290622160500142
1116	8	0.29132123721613823
1116	9	0.16488107565450605
1116	10-11	0.16143146222351135
1116	12-13	0.15211665747646208
1116	14-15	0.11144833636853946
1116	16-17	0.18350477673909893
1116	18-19	0.1451170453033086
1116	20-21	0.22189890354921005
1116	22-23	0.13735955414895784
1116	24-25	0.18240274516328014
1116	26-27	0.14886009807285916
1116	28-29	0.20266324176673578
1116	30-31	0.17909516457405772
1116	32-33	0.24312956514061312
1116	34-35	0.1290190938208582
1116	36-37	0.2735628607120688
1116	38-39	0.2083673063882614
1116	40-41	0.2440862495704792
1116	42-43	0.10471503688219741
1116	44-45	0.18005972734339082
1116	46-47	0.14568070241734432
1116	48-49	0.25469761664888324
1116	50-51	0.07923890368416409
1116	52-53	0.27523249790139204
1116	54-55	0.18685408418453164
1116	56-57	0.3166264140249666
1116	58-59	0.1264255974837809
1116	60-61	0.2765132514779651
1116	62-63	0.17665984508531096
1116	64-65	0.28491975928320556
1116	66-67	0.09767074309611701
1116	68-69	0.20688761482725226
1116	70-71	0.13357627617465795
1116	72-73	0.19575465898498123
1116	74-75	0.17047151132166505
1116	76	0.34076502511818774
2118	1	0.07032027071083036
2118	2	-0.0030147022745552476
2118	3	0.06917716137694185
2118	4	0.09424773655902641
2118	5	0.06627812514170017
2118	6	0.18863412025704918
2118	7	0.36318966967431265
2118	8	0.3582484391532077
2118	9	0.10741099222904182
2118	10-11	0.16616781658010638
2118	12-13	0.15259671254356277
2118	14-15	0.17344437613206054
2118	16-17	0.16573048317388128
2118	18-19	0.21409125697233833
2118	20-21	0.16359290883091404
2118	22-23	0.17889910026359246
2118	24-25	0.1448892350105737
2118	26-27	0.17399422143675025
2118	28-29	0.16510292615785715
2118	30-31	0.1915902348407883
2118	32-33	0.1956254774176358
2118	34-35	0.1520833449217065
2118	36-37	0.2653386111595424
2118	38-39	0.28479406424854403
2118	40-41	0.22438286024207343
2118	42-43	0.193552675597239
2118	44-45	0.1321657335580113
2118	46-47	0.2337573952425558
2118	48-49	0.2414296929240507
2118	50-51	0.1654949235117371
2118	52-53	0.2849830421295323
2118	54-55	0.24659782773904482
2118	56-57	0.28449196486999995
2118	58-59	0.22480736708736515
2118	60-61	0.235140144124955
2118	62-63	0.26206904512581275
2118	64-65	0.16341595492551164
2118	66-67	0.17755135793277077
2118	68-69	0.18166513149819963
2118	70-71	0.1946888789265202
2118	72-73	0.20459287437483908
2118	74-75	0.21086687372228852
2118	76	0.2662999258639047
1119	1	0.08870112118420792
1119	2	0.04547440826544147
1119	3	0.0871716831733238
1119	4	-0.024147096405826574
1119	5	0.08653857829533251
1119	6	0.20023993297390774
1119	7	0.3742160238549346
1119	8	0.1076320360839702
1119	9	0.12676535327112504
1119	10-11	0.1734578136601712
1119	12-13	-0.056378759757436114
1119	14-15	0.07178846803060424
1119	16-17	0.11959012318689588
1119	18-19	0.23984803834853352
1119	20-21	0.1633002947975868
1119	22-23	0.23043339654763884
1119	24-25	0.09062745642800252
1119	26-27	0.15488406553921408
1119	28-29	0.09180986874415709
1119	30-31	0.17450748274167438
1119	32-33	0.1045594964470169
1119	34-35	0.13709520820259513
1119	36-37	0.13589274835305076
1119	38-39	0.25731653766140994
1119	40-41	0.13395698683572732
1119	42-43	0.2593385395443093
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2101	22-23	-0.13493908634657714
2101	24-25	-0.004519011234805248
2101	26-27	0.011012339431545115
2101	28-29	-0.17415864711455953
2101	30-31	-0.0393625689351893
2101	32-33	-0.23189271443596482
2101	34-35	0.05606738078962792
2101	36-37	-0.4362334916239732
2101	38-39	-0.09173589817056182
2101	40-41	-0.0442724807834054
2101	42-43	0.08679980029481982
2101	44-45	-0.15409797153266425
2101	46-47	-0.10670130833205604
2101	48-49	-0.056459135834614926
2101	50-51	0.018297262666088443
2101	52-53	-0.1575772768027619
2101	54-55	0.07893674885320934
2101	56-57	-0.5371454859863292
2101	58-59	-0.008258138249111369
2101	60-61	-0.20372201830558367
2101	62-63	-0.05366093089394042
2101	64-65	-0.453341566445566
2101	66-67	-0.034972786419530166
2101	68-69	-0.12094441204450135
2101	70-71	-0.013932361076832933
2101	72-73	-0.04333691379454763
2101	74-75	0.04958939083849856
2101	76	-0.12363752689179819
2107	1	-0.10753248277193705
2107	2	-0.0589509037169762
2107	3	0.004512778585599619
2107	4	0.025783166905156918
2107	5	0.11717516619666668
2107	6	-0.008217621053702828
2107	7	-0.02960062327883861
2107	8	-0.08466159865184864
2107	9	-0.03956561246732093
2107	10-11	0.007012381850785232
2107	12-13	0.04203461824143062
2107	14-15	0.003301612296873202
2107	16-17	-0.005847380537588265
2107	18-19	0.12539791769862063
2107	20-21	-0.12968310413410933
2107	22-23	-0.08500150357920688
2107	24-25	-0.10687081966401735
2107	26-27	0.01867887650525546
2107	28-29	-0.12759083557511985
2107	30-31	0.02358817156707005
2107	32-33	-0.015437800635531573
2107	34-35	0.11625515895889293
2107	36-37	-0.013613041045459795
2107	38-39	0.1520292125706746
2107	40-41	0.051584442517814466
2107	42-43	0.1566206886545416
2107	44-45	-0.0017428220350410584
2107	46-47	-0.0384958265499975
2107	48-49	-0.12447139249346861
2107	50-51	0.006807834616502362
2107	52-53	-0.06403784004614721
2107	54-55	0.16440315187920618
2107	56-57	-0.15016937563792965
2107	58-59	0.12267311876206577
2107	60-61	-0.06897652672304844
2107	62-63	0.04827243402768033
2107	64-65	-0.19338827053220342
2107	66-67	0.019906287152821278
2107	68-69	-0.018549017859179173
2107	70-71	0.08588727185111367
2107	72-73	0.029710636696080428
2107	74-75	0.06411883678810426
2107	76	0.011258818991805697
2106	1	0.04933135297417124
2106	2	-0.011138011855017282
2106	3	-0.025451212807467982
2106	4	-0.03446355060292916
2106	5	-0.07405847013591682
2106	6	0.024047896069902208
2106	7	0.08957871714887489
2106	8	-0.07627569177655147
2106	9	-0.0427995561904595
2106	10-11	0.06850079859430025
2106	12-13	-0.0473347310176635
2106	14-15	-0.10916728270729692
2106	16-17	-0.03428100613590601
2106	18-19	0.11616955335787793
2106	20-21	0.06188567328097605
2106	22-23	-0.09650943718284566
2106	24-25	-0.04965519251411621
2106	26-27	-0.021035982168278622
2106	28-29	-0.12364818586860338
2106	30-31	-0.17070003634604802
2106	32-33	-0.08203926879133405
2106	34-35	0.00935357028647843
2106	36-37	-0.031353013038767585
2106	38-39	0.12098624376349676
2106	40-41	-0.03470246264488708
2106	42-43	0.1393784575846766
2106	44-45	0.04747516177572919
2106	46-47	-0.18557049317762875
2106	48-49	-0.09521482628984046
2106	50-51	0.06530840304505858
2106	52-53	0.04229714978068699
2106	54-55	0.151203594424004
2106	56-57	0.043038879018062914
2106	58-59	0.1111277371830468
2106	60-61	0.08866590976177235
2106	62-63	0.05049430657282983
2106	64-65	-0.07216289227105932
2106	66-67	0.0784471091053831
2106	68-69	-0.01171854374585024
2106	70-71	0.05785355891485011
2106	72-73	-0.03063043263267673
2106	74-75	0.08654072055544049
2106	76	0.028390230890259716
2105	1	0.06877015695219768
2105	2	-0.0920692246017083
2105	3	0.00868834750504277
2105	4	-0.03166168764125388
2105	5	-0.030312166892159098
2105	6	-0.05280252865462387
2105	7	-0.02034145565645673
2105	8	-0.005214887003340607
2105	9	-0.014916026482723055
2105	10-11	-0.011515437810437845
2105	12-13	-0.052698716838392556
2105	14-15	0.0041752216534050035
2105	16-17	0.03695154595260419
2105	18-19	0.0013436668365329751
2105	20-21	0.1379714215092278
2105	22-23	-0.007612667731891065
2105	24-25	0.035165181337305285
2105	26-27	0.021273579980189083
2105	28-29	-0.06121777345243373
2105	30-31	-0.13811732056499437
2105	32-33	-0.022741407721632356
2105	34-35	0.07994165703095746
2105	36-37	0.08290353412447615
2105	38-39	0.07822796033661916
2105	40-41	0.023467611001798616
2105	42-43	0.1529845671453458
2105	44-45	0.04005638389372024
2105	46-47	-0.11202222476158141
2105	48-49	0.0028906153114078847
2105	50-51	0.0589048851813061
2105	52-53	0.0934129089074176
2105	54-55	0.052482938380379096
2105	56-57	0.1470114482789029
2105	58-59	0.06214801198354536
2105	60-61	0.17807534794098956
2105	62-63	-0.010236130570170587
2105	64-65	0.017947741724285038
2105	66-67	0.0697593878535514
2105	68-69	-0.04197273695356074
2105	70-71	0.034837072848169726
2105	72-73	0.012689602152434531
2105	74-75	0.08617194854993926
2105	76	0.12683363769633615
2104	1	0.014461699330560407
2104	2	-0.04628030479546652
2104	3	-0.0224956033260284
2104	4	-0.12956360738111528
2104	5	-0.00823645580096155
2104	6	-0.04781496735657953
2104	7	0.0660523379849991
2104	8	0.07857929219932913
2104	9	-0.10267082902878855
2104	10-11	-0.11206092250804289
2104	12-13	-0.18722575566771837
2104	14-15	-0.17864062839851869
2104	16-17	-0.08692771581405623
2104	18-19	-0.1572870822438901
2104	20-21	0.01487598316884231
2104	22-23	-0.02017894370429474
2104	24-25	-0.024541246118204185
2104	26-27	-0.044683316359197534
2104	28-29	-0.15861080729047927
2104	30-31	-0.03793681319832132
2104	32-33	-0.21309037672236997
2104	34-35	-0.1179699741283855
2104	36-37	-0.07182478491999689
2104	38-39	-0.11486180533143653
2104	40-41	-0.27522632059258
2104	42-43	-0.12741386804696475
2104	44-45	-0.12447228980089164
2104	46-47	-0.25215380086385863
2104	48-49	-0.09739330504103805
2104	50-51	-0.11461404343760506
2104	52-53	-0.0802795184980809
2104	54-55	-0.10126880953410478
2104	56-57	-0.0026914227100078847
2104	58-59	-0.23617574812765696
2104	60-61	-0.05305434397668307
2104	62-63	-0.23293294005392795
2104	64-65	-0.15904729689670916
2104	66-67	-0.1041781988370829
2104	68-69	-0.07425380555191197
2104	70-71	-0.10416840634785984
2104	72-73	-0.061903571161522564
2104	74-75	-0.07986018543718387
2104	76	0.028106576341784262
2111	1	0.05955513240905219
2111	2	-0.06317284862945627
2111	3	0.03893768355589344
2111	4	-0.062207580278453634
2111	5	-0.011697234264154588
2111	6	0.032293977074651536
2111	7	0.011447463763190058
2111	8	-0.043692151923117706
2111	9	0.07965693626474746
2111	10-11	-0.07303825752707382
2111	12-13	-0.05512740572349628
2111	14-15	-0.10324343264455393
2111	16-17	-0.0535407897029927
2111	18-19	-0.24484127632682373
2111	20-21	-0.05395749029878516
2111	22-23	0.03488292247590152
2111	24-25	-0.0237309002883066
2111	26-27	-0.08130387150645646
2111	28-29	-0.12776134272606754
2111	30-31	0.014417949073916247
2111	32-33	-0.11189115125919358
2111	34-35	-0.043073254682454376
2111	36-37	-0.027738921136474914
2111	38-39	-0.06812520710119685
2111	40-41	-0.13279452903366007
2111	42-43	-0.09079489161607768
2111	44-45	-0.024616226944949915
2111	46-47	-0.09946636590465374
2111	48-49	-0.08449554048485908
2111	50-51	-0.08051435009861763
2111	52-53	-0.038329286510354166
2111	54-55	-0.15769264361955493
2111	56-57	-0.08029129927295742
2111	58-59	-0.12811182274410982
2111	60-61	0.02686694215340424
2111	62-63	-0.11510739341409959
2111	64-65	0.030030177402259994
2111	66-67	0.010720082609410042
2111	68-69	-0.021653297502403746
2111	70-71	-0.1257838659558459
2111	72-73	-0.12290143413674315
2111	74-75	-0.06550658900055595
2111	76	-0.1980367439884958
2110	1	-0.038812726219227045
2110	2	-0.0012584134888484755
2110	3	-0.01930847445417072
2110	4	-0.0923915194362408
2110	5	-0.016084557741997685
2110	6	0.0333921418965204
2110	7	0.022863796474105413
2110	8	0.13180925088883555
2110	9	0.15261461142673483
2110	10-11	0.036887378765925405
2110	12-13	0.10899963788008904
2110	14-15	0.07710214934646586
2110	16-17	-0.017826490846324816
2110	18-19	-0.11873452131140994
2110	20-21	0.024420631483344835
2110	22-23	0.11281306624009346
2110	24-25	-0.027158537003941774
2110	26-27	-0.0036439911003540715
2110	28-29	0.015104891378165064
2110	30-31	0.09493211965037318
2110	32-33	0.10148118346939583
2110	34-35	0.07397985033097143
2110	36-37	0.10670908429648307
2110	38-39	0.12672402545758388
2110	40-41	-0.007305991962709868
2110	42-43	-0.07007994464253642
2110	44-45	0.03642115748532149
2110	46-47	0.11013364340541187
2110	48-49	0.014033036409635713
2110	50-51	0.06481127826426558
2110	52-53	-0.019549076513122543
2110	54-55	0.01989768266795977
2110	56-57	0.07624339319535522
2110	58-59	0.06972829211195375
2110	60-61	-0.06441079842192465
2110	62-63	0.06946543776557235
2110	64-65	0.07917818199804572
2110	66-67	0.10585225567400869
2110	68-69	-0.008392131607756426
2110	70-71	-0.013757898594256801
2110	72-73	-0.010015964438764513
2110	74-75	0.029959118164875065
2110	76	-0.18310282477099804
2109	1	-0.0688368355161515
2109	2	-0.14402623967462347
2109	3	-0.08451034254014189
2109	4	0.002110029943025893
2109	5	0.014593269449918012
2109	6	0.09930070390896617
2109	7	-0.08215989661488976
2109	8	0.11030016778405383
2109	9	0.14066265774669517
2109	10-11	0.05024374016036148
2109	12-13	0.10222476800841207
2109	14-15	0.009332165213031374
2109	16-17	0.05859229413776035
2109	18-19	0.03578149762713423
2109	20-21	-0.08434409232469164
2109	22-23	-0.032553367748249684
2109	24-25	-0.10025234086214851
2109	26-27	-0.04342125471053038
2109	28-29	-4.042829304395923E-4
2109	30-31	-0.07867501788138043
2109	32-33	0.07651646613972929
2109	34-35	0.020294003355068924
2109	36-37	0.12073551784327918
2109	38-39	-0.036777289397569746
2109	40-41	0.02859255301055441
2109	42-43	-0.09921558572218458
2109	44-45	0.1131931537126718
2109	46-47	-0.003725623844232473
2109	48-49	-0.05119982107135712
2109	50-51	-0.2443589677906246
2109	52-53	-0.0739067373811011
2109	54-55	-0.06617097372597414
2109	56-57	-0.06326380575421098
2109	58-59	-0.10071948790731966
2109	60-61	-0.26909869314086166
2109	62-63	-0.010248108029244918
2109	64-65	-0.08024406891236424
2109	66-67	-0.012073317842421716
2109	68-69	-0.11103210685967468
2109	70-71	-0.07555706324684053
2109	72-73	-0.06415615666168151
2109	74-75	-0.14403407484485342
2109	76	-0.21628292120094983
2108	1	-0.039183343970329076
2108	2	-0.029610975769244874
2108	3	-0.026334657546691176
2108	4	0.015191976633623483
2108	5	0.00229090073995053
2108	6	-0.1274573146201945
2108	7	-0.1473493656520546
2108	8	0.1162360016412407
2108	9	0.09651077984647571
2108	10-11	-0.12671673402937245
2108	12-13	0.1278490930442686
2108	14-15	0.058133963953274304
2108	16-17	0.1062286548027771
2108	18-19	-0.005045750954884909
2108	20-21	-0.020802274546646515
2108	22-23	-0.05960325509751385
2108	24-25	-0.014391216468169432
2108	26-27	-0.16657765117662393
2108	28-29	0.008313880701457776
2108	30-31	-0.10980428549044063
2108	32-33	0.08175050519675864
2108	34-35	-0.0014552439983503973
2108	36-37	0.13861745575821516
2108	38-39	-0.010329172312445678
2108	40-41	0.06920971200926829
2108	42-43	-0.010720646050323523
2108	44-45	0.1119378323536182
2108	46-47	-0.034863152208394865
2108	48-49	0.04034365064865142
2108	50-51	-0.03383335542017818
2108	52-53	-0.11973749622436713
2108	54-55	-0.1505840370020124
2108	56-57	-0.01575631510745268
2108	58-59	-0.1828619069071422
2108	60-61	-0.29316605612874014
2108	62-63	-0.112875587822856
2108	64-65	-0.14557878396332313
2108	66-67	-0.12042842240701646
2108	68-69	-0.18501212747350593
2108	70-71	-0.07663856175229711
2108	72-73	-0.09363583654734953
2108	74-75	-0.20481666609821758
2108	76	-0.09364298983013697
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	13.0
14	59.0
15	214.0
16	535.0
17	1063.0
18	1413.0
19	1576.0
20	1783.0
21	1771.0
22	1771.0
23	1901.0
24	1999.0
25	2461.0
26	3150.0
27	4147.0
28	5432.0
29	7634.0
30	10587.0
31	15158.0
32	22324.0
33	33570.0
34	51698.0
35	89672.0
36	193532.0
37	2035894.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	11.054135242901985	27.82689352033737	24.20768728322724	36.9112839535334
2	25.618191020985314	28.400753607352897	26.773387537359	19.207667834302793
3	21.95853552720378	29.27901147282836	23.931010380178037	24.831442619789826
4	21.892936337050486	24.912668316354406	26.60699938940129	26.587395957193817
5	24.575874923675162	25.126096667416526	28.039937976025968	22.258090432882348
6	23.533438313462092	25.807637304367386	25.76023556255423	24.89868881961629
7	24.984534177459267	26.006322910306263	26.81580807918501	22.19333483304946
8	21.7492447858084	25.93674679435678	29.152874634444192	23.16113378539062
9	22.50317350644342	24.352885882315135	29.73025195230903	23.413688658932415
10-11	23.508833595783653	25.6416910370537	27.041026609248963	23.80844875791368
12-13	23.642261464794164	26.189683292091143	26.196090561429443	23.97196468168525
14-15	23.991608284860366	26.182633287270622	25.944981842722626	23.880776585146382
16-17	23.967726644599416	26.14563582607578	26.17646704373815	23.71017048558666
18-19	23.790954301507213	25.982903236173154	26.186770896937368	24.039371565382268
20-21	24.026074975094	26.1067101584343	26.123421280971815	23.743793585499887
22-23	23.7708889031719	25.91941302182087	26.275006427354825	24.03469164765241
24-25	23.772294887039237	25.806592859208795	26.306299611145036	24.114812642606935
26-27	23.718827329112706	25.811453546293023	26.266369669312596	24.20334945528168
28-29	23.717039721052803	25.84951553813028	26.29481071440049	24.138634026416426
30-31	23.759801716103738	25.843469807500725	26.199746119484523	24.19698235691101
32-33	24.179728926310375	25.96882331201594	25.95936304913713	23.892084712536555
34-35	24.038648487964778	25.888742488029052	25.842686473631776	24.229922550374393
36-37	23.8692475174342	25.832221936562007	25.960407494295723	24.33812305170807
38-39	24.171373365041617	25.9493604781952	25.94004081370312	23.939225343060063
40-41	24.187019956936723	25.939136966931258	25.990917344216985	23.88292573191503
42-43	24.03915062506026	25.827381334961597	26.11691358421442	24.016554455763732
44-45	24.167054986020503	25.834732622039397	26.067161840794423	23.931050551145677
46-47	23.97716682199441	25.91182070893724	26.014839155445575	24.096173313622778
48-49	23.88107786740367	25.866648455828006	25.839292026866346	24.412981649901983
50-51	24.334889288813187	25.77124240768712	25.9883062313205	23.905562072179194
52-53	24.257299064819872	25.957153645917025	25.92913439598933	23.856412893273774
54-55	24.13049940546968	25.884323681588846	25.941346370151365	24.043830542790115
56-57	24.167235755374875	25.792633447954493	26.166143105055113	23.87398769161552
58-59	24.303355079217148	26.089075103641097	25.817941157566604	23.78962865957515
60-61	24.271378988977084	25.89454719285278	25.814124915640967	24.019948902529165
62-63	24.310445255005302	25.811955683388497	25.983827168428835	23.893771893177362
64-65	24.236470418099433	26.18713243564611	25.736514606163833	23.839882540090628
66-67	24.216646045569945	26.15001446154835	25.842445447825945	23.790894045055758
68-69	24.52674583025356	26.129467011601378	25.66884661117717	23.674940546967896
70-71	24.290239258283254	26.08807082945014	25.817278336600573	23.804411575666034
72-73	24.544621910852587	26.145193945431757	25.685939357907255	23.6242447858084
74-75	24.757287013529584	26.043340456984925	25.84867194780988	23.350700581675614
76	24.583828775267538	26.041191310216284	25.69728765626507	23.677692258251117
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	19.5
2	10.0
3	11.0
4	11.0
5	7.5
6	8.0
7	9.0
8	6.0
9	11.5
10	18.5
11	33.0
12	46.0
13	57.0
14	75.5
15	107.5
16	174.5
17	217.0
18	314.5
19	503.5
20	772.5
21	950.0
22	1198.0
23	1866.0
24	2893.0
25	3500.0
26	4260.5
27	5919.0
28	7710.5
29	8604.0
30	9915.0
31	13295.0
32	18250.0
33	21136.0
34	26604.0
35	37458.0
36	50422.0
37	58000.0
38	66728.0
39	88122.0
40	112810.5
41	128780.0
42	132727.0
43	140050.5
44	153652.0
45	167420.5
46	174911.0
47	180634.0
48	181936.5
49	173162.5
50	168809.0
51	158846.0
52	133672.5
53	111650.5
54	104839.0
55	96705.0
56	84317.0
57	68828.0
58	57593.0
59	49855.5
60	41795.0
61	34943.0
62	28414.0
63	27825.5
64	24715.5
65	19351.5
66	14429.0
67	12349.0
68	9071.5
69	7223.5
70	8372.0
71	8091.0
72	8183.0
73	6307.5
74	3498.0
75	2656.0
76	2370.0
77	1384.5
78	416.5
79	148.0
80	84.0
81	18.0
82	12.5
83	9.0
84	6.0
85	5.5
86	8.5
87	9.0
88	7.0
89	6.5
90	7.5
91	7.5
92	8.0
93	8.0
94	7.5
95	9.5
96	12.0
97	13.5
98	18.0
99	77.5
100	134.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.034193527653694E-5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	2489360.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.937564353126895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.88470850689994	47.72267961145422
2	4.793332504588817	4.979080308460333
3	0.8998581152061776	1.4020931630161297
4	0.3823681930235704	0.7943709052700212
5	0.22649028955590622	0.5881676994584111
6	0.14087634510742872	0.43900645439102365
7	0.1559766692886835	0.5670733809137173
8	0.09486498956276926	0.39416452002200314
9	0.06459558877964576	0.3019443794253876
>10	0.8595751348868681	10.259409829019257
>50	0.2509222512884368	9.404645800779194
>100	0.2431363749994193	22.02654996303374
>500	0.0031377383098434818	1.030777786594394
>1k	1.5729850249710954E-4	0.09003619816237061
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	2.0085483819134235E-4	0.0	4.017096763826847E-5
2	2.811967734678793E-4	0.0	4.017096763826847E-5
3	3.213677411061478E-4	0.0	4.017096763826847E-5
4	4.017096763826847E-4	4.017096763826847E-5	4.017096763826847E-5
5	4.418806440209532E-4	4.017096763826847E-5	4.017096763826847E-5
6	6.427354822122956E-4	4.017096763826847E-5	4.017096763826847E-5
7	7.632483851271009E-4	4.017096763826847E-5	4.017096763826847E-5
8	8.837612880419064E-4	4.017096763826847E-5	4.017096763826847E-5
9	9.641032233184433E-4	4.017096763826847E-5	4.017096763826847E-5
10	0.0010042741909567117	4.017096763826847E-5	4.017096763826847E-5
11	0.0010846161262332487	4.017096763826847E-5	4.017096763826847E-5
12	0.0011649580615097856	4.017096763826847E-5	4.017096763826847E-5
13	0.0012452999967863226	4.017096763826847E-5	4.017096763826847E-5
14	0.0014059838673393965	4.017096763826847E-5	4.017096763826847E-5
15	0.0016871806408072757	4.017096763826847E-5	4.017096763826847E-5
16	0.0018478645113603497	4.017096763826847E-5	4.017096763826847E-5
17	0.0019282064466368866	4.017096763826847E-5	4.017096763826847E-5
18	0.0022495741877430345	4.017096763826847E-5	4.017096763826847E-5
19	0.002410258058296108	4.017096763826847E-5	4.017096763826847E-5
20	0.00289230966995533	4.017096763826847E-5	4.017096763826847E-5
21	0.003253848378699746	4.017096763826847E-5	4.017096763826847E-5
22	0.004057267731465116	4.017096763826847E-5	4.017096763826847E-5
23	0.0050213709547835585	4.017096763826847E-5	4.017096763826847E-5
24	0.005945303210463734	4.017096763826847E-5	4.017096763826847E-5
25	0.007752996754185815	4.017096763826847E-5	4.017096763826847E-5
26	0.010283767715396729	4.017096763826847E-5	4.017096763826847E-5
27	0.013537616094096474	4.017096763826847E-5	4.017096763826847E-5
28	0.017675225760838128	4.017096763826847E-5	4.017096763826847E-5
29	0.02265642574798342	4.017096763826847E-5	4.017096763826847E-5
30	0.028280361217341003	4.017096763826847E-5	4.017096763826847E-5
31	0.03647523861554777	4.017096763826847E-5	4.017096763826847E-5
32	0.0444692611755632	4.017096763826847E-5	4.017096763826847E-5
33	0.05459234502040685	4.017096763826847E-5	4.017096763826847E-5
34	0.06696500305299354	4.017096763826847E-5	4.017096763826847E-5
35	0.08010090947070733	8.034193527653694E-5	4.017096763826847E-5
36	0.0955667320114407	8.034193527653694E-5	4.017096763826847E-5
37	0.11215734164604557	8.034193527653694E-5	4.017096763826847E-5
38	0.1312385512742231	8.034193527653694E-5	4.017096763826847E-5
39	0.15080181251405983	8.034193527653694E-5	4.017096763826847E-5
40	0.17410097374425554	8.034193527653694E-5	4.017096763826847E-5
41	0.19784201561847223	8.034193527653694E-5	4.017096763826847E-5
42	0.22391297361570844	8.034193527653694E-5	4.017096763826847E-5
43	0.25259504450943215	8.034193527653694E-5	4.017096763826847E-5
44	0.28453096378185555	1.2051290291480541E-4	4.017096763826847E-5
45	0.318716457242022	1.2051290291480541E-4	4.017096763826847E-5
46	0.35527203779284633	1.2051290291480541E-4	4.017096763826847E-5
47	0.3935549699521162	1.2051290291480541E-4	4.017096763826847E-5
48	0.43617636661631903	1.2051290291480541E-4	4.017096763826847E-5
49	0.4808063116624353	1.2051290291480541E-4	4.017096763826847E-5
50	0.5302166018575055	1.2051290291480541E-4	4.017096763826847E-5
51	0.5813140726933831	1.2051290291480541E-4	4.017096763826847E-5
52	0.6342995790082592	1.2051290291480541E-4	4.017096763826847E-5
53	0.692185943375004	1.2051290291480541E-4	4.017096763826847E-5
54	0.7530449593469808	1.2051290291480541E-4	4.017096763826847E-5
55	0.8198492785294212	1.2051290291480541E-4	4.017096763826847E-5
56	0.8883006073850307	1.2051290291480541E-4	4.017096763826847E-5
57	0.9603271523604461	1.2051290291480541E-4	4.017096763826847E-5
58	1.040829771507536	1.2051290291480541E-4	4.017096763826847E-5
59	1.1218546132339235	1.2051290291480541E-4	4.017096763826847E-5
60	1.2137256162226435	1.2051290291480541E-4	4.017096763826847E-5
61	1.308810296622425	1.2051290291480541E-4	4.017096763826847E-5
62	1.4107642124883504	1.2051290291480541E-4	4.017096763826847E-5
63	1.5226805283285665	1.2051290291480541E-4	4.017096763826847E-5
64	1.638453257062056	1.2051290291480541E-4	4.017096763826847E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTGTGT	2390	0.0	20.648535	2
CTTTGTG	3135	0.0	16.746412	1
GTGTTTG	3210	0.0	15.919003	5
TGTGTTT	3400	0.0	14.926471	4
TTGTGTT	4380	0.0	11.586759	3
TGTTTGA	5370	0.0	9.90689	1
GTTTGAC	1045	0.0	9.043062	2
TTGAGGG	785	8.785719E-10	8.917197	9
CCTCGCA	375	0.0034100418	8.400001	48
ACTTTGT	1905	0.0	8.267716	3
TAACTTT	1745	0.0	7.8223495	1
GAGTGTA	1170	4.0636223E-9	6.8803425	70
GTAGGGA	1850	0.0	6.810811	69
TGTAGGG	1845	0.0	6.6395664	68
GCCTCGG	785	2.81942E-4	6.242038	53
CGTGTAG	2360	0.0	6.2288136	70
TTTGACG	800	3.5990085E-4	6.1249995	3
GCGGGGG	745	0.0010185948	6.1073823	47
GTTTGAG	5360	0.0	6.072761	7
GTGTACA	645	0.009656293	5.968992	16
>>END_MODULE