FastQCFastQC Report
Mon 15 Sep 2014
pBOB11_1_GTTTCG_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamepBOB11_1_GTTTCG_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2489360
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTGT23900.020.6485352
CTTTGTG31350.016.7464121
GTGTTTG32100.015.9190035
TGTGTTT34000.014.9264714
TTGTGTT43800.011.5867593
TGTTTGA53700.09.906891
GTTTGAC10450.09.0430622
TTGAGGG7858.785719E-108.9171979
CCTCGCA3750.00341004188.40000148
ACTTTGT19050.08.2677163
TAACTTT17450.07.82234951
GAGTGTA11704.0636223E-96.880342570
GTAGGGA18500.06.81081169
TGTAGGG18450.06.639566468
GCCTCGG7852.81942E-46.24203853
CGTGTAG23600.06.228813670
TTTGACG8003.5990085E-46.12499953
GCGGGGG7450.00101859486.107382347
GTTTGAG53600.06.0727617
GTGTACA6450.0096562935.96899216
Produced by FastQC (version 0.11.2)