##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename pBOB11_1_GTTTCG_L001_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2489360 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.76182593116303 34.0 34.0 34.0 34.0 34.0 2 33.81892735482212 34.0 34.0 34.0 34.0 34.0 3 33.76220514509753 34.0 34.0 34.0 34.0 34.0 4 33.80130877012566 34.0 34.0 34.0 34.0 34.0 5 33.834522527878654 34.0 34.0 34.0 34.0 34.0 6 37.60765980010927 38.0 38.0 38.0 37.0 38.0 7 37.57890341292541 38.0 38.0 38.0 37.0 38.0 8 37.52828196805605 38.0 38.0 38.0 37.0 38.0 9 37.5477717164251 38.0 38.0 38.0 37.0 38.0 10-11 37.54975294854903 38.0 38.0 38.0 37.0 38.0 12-13 37.48142815824147 38.0 38.0 38.0 37.0 38.0 14-15 37.4500935983546 38.0 38.0 38.0 37.0 38.0 16-17 37.507653171899605 38.0 38.0 38.0 37.0 38.0 18-19 37.445444612269824 38.0 38.0 38.0 37.0 38.0 20-21 37.44875068290645 38.0 38.0 38.0 37.0 38.0 22-23 37.25719622714272 38.0 38.0 38.0 37.0 38.0 24-25 37.41620938715172 38.0 38.0 38.0 37.0 38.0 26-27 37.43940510814025 38.0 38.0 38.0 37.0 38.0 28-29 37.506659944724746 38.0 38.0 38.0 37.0 38.0 30-31 37.374361884179066 38.0 38.0 38.0 37.0 38.0 32-33 37.37286451136035 38.0 38.0 38.0 37.0 38.0 34-35 37.426490342899385 38.0 38.0 38.0 37.0 38.0 36-37 37.48729030754893 38.0 38.0 38.0 37.0 38.0 38-39 37.42831611337854 38.0 38.0 38.0 37.0 38.0 40-41 37.410997806665165 38.0 38.0 38.0 37.0 38.0 42-43 37.27932802005334 38.0 38.0 38.0 37.0 38.0 44-45 37.36602781437799 38.0 38.0 38.0 37.0 38.0 46-47 37.35714239804608 38.0 38.0 38.0 37.0 38.0 48-49 37.367387400777716 38.0 38.0 38.0 37.0 38.0 50-51 37.31473430922004 38.0 38.0 38.0 37.0 38.0 52-53 37.41870340167754 38.0 38.0 38.0 37.0 38.0 54-55 37.34824995179484 38.0 38.0 38.0 37.0 38.0 56-57 37.301902296172514 38.0 38.0 38.0 37.0 38.0 58-59 37.39999618375808 38.0 38.0 38.0 37.0 38.0 60-61 37.27336463990744 38.0 38.0 38.0 37.0 38.0 62-63 37.33118150850018 38.0 38.0 38.0 37.0 38.0 64-65 37.3380800687727 38.0 38.0 38.0 37.0 38.0 66-67 37.35968120320082 38.0 38.0 38.0 37.0 38.0 68-69 37.353834720570745 38.0 38.0 38.0 37.0 38.0 70-71 37.38921228749558 38.0 38.0 38.0 37.0 38.0 72-73 37.305291520712146 38.0 38.0 38.0 37.0 38.0 74-75 37.42039620625381 38.0 38.0 38.0 37.0 38.0 76 37.078122489314524 38.0 38.0 38.0 36.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.037076559267269715 1101 2 0.03572460551597345 1101 3 -0.03170243981262644 1101 4 0.04692653687533266 1101 5 0.0016231556865662355 1101 6 0.06366654722911846 1101 7 0.017508393935742106 1101 8 0.006501611625488124 1101 9 0.0715261840718 1101 10-11 0.03051033853184748 1101 12-13 0.08400401191500606 1101 14-15 0.011683307341975535 1101 16-17 0.10040436688149157 1101 18-19 0.07223153933473725 1101 20-21 0.02290102473704536 1101 22-23 0.2771837757587292 1101 24-25 0.09532796070200078 1101 26-27 0.042538556671154026 1101 28-29 0.053688889122142314 1101 30-31 0.07756738871651692 1101 32-33 0.11784664348589047 1101 34-35 0.077484685534003 1101 36-37 0.07364771054259478 1101 38-39 0.10350399172703817 1101 40-41 0.06216865723887821 1101 42-43 0.13637477196084546 1101 44-45 0.13754936022299802 1101 46-47 0.14088322526701802 1101 48-49 0.09234582255331247 1101 50-51 0.12223419966142046 1101 52-53 0.006957109583204613 1101 54-55 0.05988625085311128 1101 56-57 0.07169958718576908 1101 58-59 0.0743907938513999 1101 60-61 0.057782031311923276 1101 62-63 0.07706578051825375 1101 64-65 0.07091860901638114 1101 66-67 0.09084857808257141 1101 68-69 0.18206346429273168 1101 70-71 0.08804525660440277 1101 72-73 0.18841405153344226 1101 74-75 0.10067718778325485 1101 76 0.16126890599309007 1102 1 0.03627332530722782 1102 2 0.03626364893725764 1102 3 0.012000056591041641 1102 4 -0.0024221077097763555 1102 5 -0.01694861840072548 1102 6 0.008544010286634318 1102 7 0.017444183422725246 1102 8 -0.0939590074306409 1102 9 0.04431649410524585 1102 10-11 0.03769230471822027 1102 12-13 0.05474333076916338 1102 14-15 0.00881472636272207 1102 16-17 0.035687524865522846 1102 18-19 0.05464171681384755 1102 20-21 -0.1283129284826856 1102 22-23 0.23264433164218445 1102 24-25 -0.06170412831010452 1102 26-27 -0.08978866154712506 1102 28-29 -0.10641314377458855 1102 30-31 -0.06455504624720731 1102 32-33 0.1504203930730057 1102 34-35 0.15234699166157384 1102 36-37 0.12161120395548863 1102 38-39 0.1081041357607404 1102 40-41 0.08914621018210056 1102 42-43 0.11345597321636092 1102 44-45 0.11473966971495742 1102 46-47 0.04857075335629446 1102 48-49 0.012702021688028253 1102 50-51 0.0801875719091214 1102 52-53 -0.03480104465558753 1102 54-55 0.07370112242305993 1102 56-57 0.09218472626492513 1102 58-59 0.08646169344834931 1102 60-61 0.09201631981611769 1102 62-63 0.061874398895731986 1102 64-65 0.028323123516315718 1102 66-67 0.058407829540506384 1102 68-69 0.10060272701345951 1102 70-71 0.09761056993184525 1102 72-73 0.10628842183723464 1102 74-75 0.05825073431534378 1102 76 0.12081059725197463 1103 1 0.02068682875145811 1103 2 -0.009394895135450554 1103 3 -0.011092823774731642 1103 4 -0.027446294093770973 1103 5 0.05884011353506935 1103 6 0.03838361269969681 1103 7 0.04175556916299428 1103 8 -0.0714256697561595 1103 9 0.00773540227606162 1103 10-11 0.0421210874454232 1103 12-13 0.07460825911699942 1103 14-15 0.1380744369633362 1103 16-17 0.14105259972703266 1103 18-19 0.12775093461689124 1103 20-21 -0.0024226835025231708 1103 22-23 0.29683615757463144 1103 24-25 0.01701574917656501 1103 26-27 0.07997289326526413 1103 28-29 0.006382151043482054 1103 30-31 0.054450044521495045 1103 32-33 0.06445122838699291 1103 34-35 0.06870146404956046 1103 36-37 0.08301089790470684 1103 38-39 0.05704551633814248 1103 40-41 0.04836209458978402 1103 42-43 0.10523312592081879 1103 44-45 0.11647742652086635 1103 46-47 0.06546087275330592 1103 48-49 -0.022832369489528048 1103 50-51 0.022817410930130677 1103 52-53 -0.150403994929313 1103 54-55 0.020700150316471877 1103 56-57 -0.043934498654493837 1103 58-59 -0.06406238363178574 1103 60-61 -0.052423195618978013 1103 62-63 0.05033487741403064 1103 64-65 -0.17575053797663998 1103 66-67 -0.006670404109271999 1103 68-69 -0.10494008288621615 1103 70-71 -0.03287654151621666 1103 72-73 -0.14107345995646625 1103 74-75 0.014802802483735888 1103 76 -0.1107842622111832 2116 1 -0.0397712750640693 2116 2 -0.018917219185084377 2116 3 0.06565759955256567 2116 4 0.0214052165283789 2116 5 0.03899605154239083 2116 6 -0.005073928824998575 2116 7 0.07107335819680571 2116 8 -0.02464799164490472 2116 9 0.044037183721933104 2116 10-11 0.029903330549089446 2116 12-13 0.05511769783304743 2116 14-15 0.15943743132985588 2116 16-17 0.1527040658440697 2116 18-19 0.14646363342440338 2116 20-21 0.0333652776805522 2116 22-23 0.38915580727157817 2116 24-25 0.10927823876865972 2116 26-27 0.11756809305781957 2116 28-29 0.07354189497357311 2116 30-31 0.09873024098766336 2116 32-33 0.07514959314593028 2116 34-35 0.028526875733348334 2116 36-37 -0.04548551203151163 2116 38-39 -0.016935486691238566 2116 40-41 -0.0718560425310173 2116 42-43 0.008600595166086578 2116 44-45 0.03870469592530412 2116 46-47 -0.03974304158170128 2116 48-49 0.05602717320205386 2116 50-51 -0.02142769408217049 2116 52-53 -0.13710347185631377 2116 54-55 0.06294278961678401 2116 56-57 0.06707925241388324 2116 58-59 -0.04273717033597535 2116 60-61 -0.12060440774883574 2116 62-63 0.013133062819207453 2116 64-65 -0.027048416029870737 2116 66-67 0.05513107136395945 2116 68-69 -0.03948352157743784 2116 70-71 -0.019286034070574942 2116 72-73 0.08904014532088667 2116 74-75 0.09190451965466195 2116 76 -0.3983787608643041 1117 1 -0.044989607135505594 1117 2 0.027054353495344685 1117 3 0.05096404634095819 1117 4 -0.018479256657073506 1117 5 -0.0427278228001029 1117 6 -0.09003638796976787 1117 7 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0.13197299349507574 1117 70-71 0.2381639760917622 1117 72-73 0.23196289846599427 1117 74-75 0.22224450998825063 1117 76 0.11071372784556388 2117 1 0.03230474959084262 2117 2 0.028733666808449243 2117 3 -0.036291766363305555 2117 4 -0.002336610524345417 2117 5 -0.03310809784886004 2117 6 -0.07209499167900901 2117 7 0.023741081348667592 2117 8 -0.03601544022212977 2117 9 0.029156992341377475 2117 10-11 9.850886634410472E-5 2117 12-13 0.11429009805502943 2117 14-15 0.10294726699131473 2117 16-17 -0.031945397920146945 2117 18-19 0.09528456359582549 2117 20-21 -0.04498525156999733 2117 22-23 0.32037681053056133 2117 24-25 0.05887542544944324 2117 26-27 0.08094800898140164 2117 28-29 0.07867027060828491 2117 30-31 0.14208516541760474 2117 32-33 0.12548476004050713 2117 34-35 0.06499204398269143 2117 36-37 0.08078492642577118 2117 38-39 0.0325023081014848 2117 40-41 -0.03899414932450185 2117 42-43 0.07072804580947434 2117 44-45 0.0585103466443897 2117 46-47 -0.11546896308502141 2117 48-49 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38-39 -0.05061146485687118 2104 40-41 0.045196921816106794 2104 42-43 -0.15024625271949077 2104 44-45 -0.1265669273398089 2104 46-47 0.032123820280176574 2104 48-49 -0.11696458329247861 2104 50-51 -0.055022002157265604 2104 52-53 -0.15575709606819998 2104 54-55 -0.06988575973393552 2104 56-57 -0.2356846149642351 2104 58-59 -0.044028001223828994 2104 60-61 -0.06805062836377118 2104 62-63 -0.05914436678283863 2104 64-65 -0.49461328739577937 2104 66-67 -0.03377559893905868 2104 68-69 -0.4089156816330757 2104 70-71 -0.08504482312116579 2104 72-73 -0.4407702230112278 2104 74-75 -0.13197518658430596 2104 76 -0.36212906620327345 2111 1 0.030713427550480787 2111 2 -0.021021650509453593 2111 3 0.004739065160549671 2111 4 -0.03482371166487752 2111 5 -0.030093552142680835 2111 6 -0.04980350272282408 2111 7 0.02719529738431703 2111 8 0.05109739651623357 2111 9 -7.583745847199452E-4 2111 10-11 0.004467126475020677 2111 12-13 0.040925158470209055 2111 14-15 -0.07756717501030863 2111 16-17 -0.05845232663119759 2111 18-19 -0.012473954751186511 2111 20-21 -0.007740259668189253 2111 22-23 0.27663903623434294 2111 24-25 0.08344494994861407 2111 26-27 0.062253913008355255 2111 28-29 0.060172589663707754 2111 30-31 -0.07087482661493283 2111 32-33 0.0013314995926094753 2111 34-35 -0.027881545762490134 2111 36-37 -0.023579081065342677 2111 38-39 0.07819714197049876 2111 40-41 0.03009654610018231 2111 42-43 0.04378992328769726 2111 44-45 -0.039094849724911285 2111 46-47 0.07963728626654643 2111 48-49 -0.12946128423957504 2111 50-51 0.04815168673271586 2111 52-53 -0.08961433571790423 2111 54-55 0.07382864601585482 2111 56-57 -0.04182251565406858 2111 58-59 0.11083604250772794 2111 60-61 -0.08566487774634624 2111 62-63 0.04052758988381555 2111 64-65 -0.14766359495759218 2111 66-67 0.1130029327841271 2111 68-69 -0.06604931588832841 2111 70-71 0.09258542515891577 2111 72-73 -0.14592994174711293 2111 74-75 0.09373048106321136 2111 76 -0.028016914934759996 2110 1 -0.008475748336479683 2110 2 -0.002012874666540654 2110 3 0.026881755197941004 2110 4 -0.0020974432072264904 2110 5 0.005469389113827106 2110 6 0.017401985654167618 2110 7 -0.034793747094532534 2110 8 0.04673196825725512 2110 9 0.020238128344040263 2110 10-11 -0.08295257073986306 2110 12-13 -0.03320218596265789 2110 14-15 -0.14441580811847388 2110 16-17 -0.1389134151013991 2110 18-19 -0.21788779140715064 2110 20-21 -0.0850850513154029 2110 22-23 0.14005268208910593 2110 24-25 -0.07972967463346947 2110 26-27 -0.10719642868124879 2110 28-29 -0.06793783086892802 2110 30-31 -0.3385114024124576 2110 32-33 -0.07114206584047622 2110 34-35 -0.030187627490711577 2110 36-37 -0.02745271786947967 2110 38-39 0.03971385690017826 2110 40-41 -0.047657193957384436 2110 42-43 0.04395432142263189 2110 44-45 0.018657193913178105 2110 46-47 0.11478541432192912 2110 48-49 0.007409684751735313 2110 50-51 0.08966587267261161 2110 52-53 -0.04262588507786802 2110 54-55 0.02637565794866248 2110 56-57 -0.03093302514752594 2110 58-59 0.0546358225067749 2110 60-61 -0.04214875122767836 2110 62-63 0.0701238540074911 2110 64-65 0.09364503811561065 2110 66-67 0.14182318159293317 2110 68-69 0.057344225923678493 2110 70-71 0.11260076009182285 2110 72-73 0.00753951207664727 2110 74-75 0.09493421211890762 2110 76 -0.09979312224678694 2109 1 0.04913597996877428 2109 2 -0.05200763587538404 2109 3 0.05529296827396735 2109 4 -5.740769319402261E-4 2109 5 0.04705349279375781 2109 6 0.0018521858320923457 2109 7 -0.03314393503955415 2109 8 0.06706481155098487 2109 9 0.008542413982844721 2109 10-11 -0.001913832457930198 2109 12-13 0.03599912368084546 2109 14-15 -0.10351819870826517 2109 16-17 -0.018167060178093664 2109 18-19 -0.015080296986148767 2109 20-21 -0.18408947289133692 2109 22-23 0.04322577446338016 2109 24-25 -0.09496261354645696 2109 26-27 -0.19156469470832604 2109 28-29 -0.11735935612985315 2109 30-31 -0.6874478056593105 2109 32-33 -0.11950952189071984 2109 34-35 -0.0804070505034673 2109 36-37 -0.11307702705479272 2109 38-39 -0.07879974604179552 2109 40-41 -0.15852191298807128 2109 42-43 -0.029717310242759254 2109 44-45 -0.041368449924299 2109 46-47 0.001428130031641217 2109 48-49 -0.07474620991073522 2109 50-51 0.031775013612197256 2109 52-53 -0.02087322985222073 2109 54-55 -0.03562500563155169 2109 56-57 -0.0210034348626138 2109 58-59 0.021130246949105924 2109 60-61 -0.10963125782729577 2109 62-63 0.016161613295984978 2109 64-65 0.07666524736325186 2109 66-67 0.10063999472859564 2109 68-69 -0.0342170858479065 2109 70-71 0.0527064931932415 2109 72-73 -0.305363146478669 2109 74-75 -0.11126953903652748 2109 76 -0.4217772343232582 2108 1 0.019616585775388273 2108 2 -0.06255769470807593 2108 3 -0.06686889148393504 2108 4 -0.016474463418866492 2108 5 0.001163744547540091 2108 6 -0.1397484843412542 2108 7 -0.1219466494027941 2108 8 -0.02459124373920929 2108 9 -0.01412344138364574 2108 10-11 0.0030124319077273753 2108 12-13 0.05691233336701629 2108 14-15 -0.11218230924209394 2108 16-17 -0.006474353104024999 2108 18-19 -0.021256364347351564 2108 20-21 -0.047569928258177185 2108 22-23 0.10087513294523376 2108 24-25 0.022069988900341286 2108 26-27 -0.007959327321877652 2108 28-29 -0.0914420317369391 2108 30-31 -0.08808653664613786 2108 32-33 -0.017678510971308015 2108 34-35 -0.13931324145809754 2108 36-37 -0.2746040377091461 2108 38-39 -0.22613142140352238 2108 40-41 -0.29396549986615383 2108 42-43 -0.10758498996007404 2108 44-45 -0.27715858555291106 2108 46-47 -0.2654580063169334 2108 48-49 -0.29038212910668193 2108 50-51 -0.1976562658601111 2108 52-53 -0.12530690471964334 2108 54-55 -0.17396080823667148 2108 56-57 -0.2775162455252129 2108 58-59 -0.1364132887973497 2108 60-61 -0.3934728626614188 2108 62-63 -0.13694173300381607 2108 64-65 -0.04895148852362041 2108 66-67 -0.17810382873391006 2108 68-69 -0.49939148852144655 2108 70-71 -0.20930690579631062 2108 72-73 -1.0075508250139507 2108 74-75 -0.6050190577188204 2108 76 -0.9463826657361523 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 2.0 14 1.0 15 3.0 16 2.0 17 15.0 18 30.0 19 111.0 20 204.0 21 359.0 22 557.0 23 714.0 24 943.0 25 1156.0 26 1544.0 27 2296.0 28 3422.0 29 5727.0 30 8935.0 31 14714.0 32 23151.0 33 36859.0 34 58735.0 35 97784.0 36 202237.0 37 2029859.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 11.097390493942218 28.91634797699007 23.3468039978147 36.63945753125301 2 25.2727206992962 27.442595687244914 27.75596940579105 19.528714207667836 3 21.205972619468458 28.787439341838866 24.88149564546711 25.125092393225568 4 21.54160105408619 24.9243178969695 27.21273741041874 26.321343638525562 5 24.451385094964166 25.19205739627856 28.142735482212295 22.213822026544975 6 23.502546839348266 25.874280939679274 25.950886974965453 24.672285246007007 7 25.033864125719063 26.194001671112254 26.741652472924766 22.030481730243917 8 21.70071825690137 25.923932255680178 29.10555323456631 23.26979625285214 9 22.477825625863677 24.36594144679757 29.725873316836456 23.430359610502297 10-11 23.508371629655816 25.712070572355948 26.941744062730983 23.837813735257253 12-13 23.645193945431757 26.291094899893945 26.11520631808979 23.9485048365845 14-15 23.994641192917054 26.30244319825176 25.81980910756178 23.883106501269403 16-17 23.917472764083943 26.22342690490729 26.11239435035511 23.746705980653662 18-19 23.784004724105795 26.03536651990873 26.103114856830672 24.077513899154805 20-21 23.996951023556257 26.09017980525115 26.093112285888743 23.819756885303853 22-23 23.48617717003567 25.99226307163287 26.202196548510457 24.319363209821 24-25 23.76757479834174 25.847968955876212 26.230296140373426 24.15416010540862 26-27 23.706956808175594 25.89412539769258 26.22244271620015 24.176475077931677 28-29 23.702055950123725 25.9039070283125 26.26855898704888 24.125478034514895 30-31 23.723567503294017 25.968682713629203 26.097952887489157 24.209796895587623 32-33 24.13182504740174 26.080900311726708 25.84907365748626 23.93820098338529 34-35 24.068234405630363 26.003470771603947 25.73822187228846 24.19007295047723 36-37 23.88455265610438 26.033237458623905 25.855882636500947 24.22632724877077 38-39 24.175169521483433 26.095502458463223 25.910394639586077 23.818933380467268 40-41 24.140160523186683 26.029943439277563 25.984329305524312 23.84556673201144 42-43 24.055198926631743 25.905614294437125 26.031871645724202 24.007315133206927 44-45 24.119653244207345 25.93164508146672 25.971936562007908 23.976765112318024 46-47 23.933661664042162 26.02265240865122 26.07252466497413 23.971161262332487 48-49 23.830623132050004 26.05715926985249 25.856625799402256 24.255591798695246 50-51 24.289696950220137 25.889987788025838 25.859256997782566 23.961058263971463 52-53 24.198388340778354 26.09070202783045 25.829510396246423 23.881399235144777 54-55 24.0613450846804 25.993628884532573 25.926904907285408 24.018121123501622 56-57 24.13578188771411 25.901777967027666 25.973583571681075 23.988856573577145 58-59 24.184388758556416 26.133383680946103 25.829992447858086 23.852235112639395 60-61 24.1351391522319 25.986538708744416 25.794099688273292 24.084222450750396 62-63 24.182380210174504 25.851825368769482 25.951991676575503 24.013802744480508 64-65 24.062530128225728 26.286575666034643 25.66669746440852 23.984196741331104 66-67 24.021857023491982 26.28219703056207 25.72404152071215 23.971904425233795 68-69 24.26708069543979 26.276352154770706 25.621906835491853 23.83466031429765 70-71 24.03282369765723 26.228187164572418 25.70385560947392 24.03513352829643 72-73 24.185774656939937 26.338737667512934 25.577276890445738 23.89821078510139 74-75 24.33739997429058 26.195206800141403 25.731171867467946 23.736221358100074 76 24.127165215155703 26.290733361185204 25.528328566378505 24.053772857280585 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 56.0 1 42.0 2 53.0 3 63.5 4 49.0 5 54.5 6 46.0 7 32.0 8 32.0 9 30.5 10 34.0 11 44.0 12 49.0 13 65.5 14 79.0 15 111.0 16 193.5 17 241.0 18 307.0 19 481.0 20 759.0 21 929.0 22 1187.0 23 1878.0 24 2916.0 25 3521.0 26 4349.0 27 6012.0 28 7613.0 29 8379.0 30 9787.0 31 13303.0 32 18324.5 33 21238.0 34 26651.0 35 37769.5 36 50766.5 37 58058.0 38 67043.0 39 88671.5 40 112976.5 41 128940.5 42 133243.0 43 140547.5 44 153982.0 45 168075.0 46 176038.0 47 181821.0 48 182714.5 49 173099.5 50 168374.0 51 158392.0 52 133574.5 53 111631.0 54 104523.0 55 96749.5 56 84203.0 57 68293.0 58 57156.0 59 49348.5 60 41287.5 61 34594.0 62 28154.0 63 27508.5 64 24225.5 65 19024.0 66 14402.0 67 12344.0 68 9012.5 69 7059.0 70 8143.5 71 7850.0 72 7805.5 73 5982.5 74 3343.0 75 2482.0 76 2211.0 77 1268.5 78 370.5 79 144.0 80 73.5 81 1.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 1.0 94 1.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 2489360.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.368824098066135 #Duplication Level Percentage of deduplicated Percentage of total 1 91.36289458204416 46.01841566293142 2 4.99141946677152 5.028238582429554 3 1.1136903228882504 1.6828581591983027 4 0.500907543985996 1.0092049588969982 5 0.1414140805759548 0.35614304747600084 6 0.17517541962138158 0.5294027938328574 7 0.10136672301435475 0.3574005848635198 8 0.10031699759030079 0.4042279364537587 9 0.0995366060735074 0.4512187622371495 >10 0.875843215771847 10.12111496947202 >50 0.26682773984138747 9.66416041880239 >100 0.2678251186161876 23.46170431748253 >500 0.0027005190458147255 0.8707866013108387 >1k 8.166415933442231E-5 0.045123204612652616 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.213677411061478E-4 0.0 0.0 2 4.017096763826847E-4 0.0 0.0 3 4.418806440209532E-4 0.0 0.0 4 6.02564514574027E-4 0.0 0.0 5 6.82906449850564E-4 0.0 0.0 6 9.239322556801749E-4 0.0 0.0 7 0.0010444451585949802 0.0 0.0 8 0.001205129029148054 0.0 0.0 9 0.0013256419320628596 0.0 0.0 10 0.0014059838673393965 0.0 0.0 11 0.0014863258026159335 0.0 0.0 12 0.0016068387055307388 0.0 4.017096763826847E-5 13 0.0016871806408072757 0.0 4.017096763826847E-5 14 0.0018478645113603497 0.0 4.017096763826847E-5 15 0.002209403220104766 0.0 4.017096763826847E-5 16 0.0023700870906578396 0.0 4.017096763826847E-5 17 0.0025307709612109137 0.0 4.017096763826847E-5 18 0.0028521387023170614 0.0 4.017096763826847E-5 19 0.003012822572870135 0.0 4.017096763826847E-5 20 0.0036153870874441624 0.0 4.017096763826847E-5 21 0.00393675482855031 0.0 4.017096763826847E-5 22 0.004780345148953948 0.0 4.017096763826847E-5 23 0.005704277404634123 0.0 4.017096763826847E-5 24 0.0066683806279525665 0.0 4.017096763826847E-5 25 0.008476074171674647 0.0 4.017096763826847E-5 26 0.010886332229970756 0.0 4.017096763826847E-5 27 0.014260693511585307 0.0 4.017096763826847E-5 28 0.01863932898415657 0.0 4.017096763826847E-5 29 0.023580358003663593 0.0 4.017096763826847E-5 30 0.029525661214127325 0.0 4.017096763826847E-5 31 0.037680367644695824 0.0 4.017096763826847E-5 32 0.04595558697817913 0.0 4.017096763826847E-5 33 0.05603849985538452 0.0 4.017096763826847E-5 34 0.06845132885560948 0.0 4.017096763826847E-5 35 0.08150689333804673 4.017096763826847E-5 4.017096763826847E-5 36 0.09689237394350356 4.017096763826847E-5 4.017096763826847E-5 37 0.11324195777227881 4.017096763826847E-5 4.017096763826847E-5 38 0.13248385127100942 4.017096763826847E-5 4.017096763826847E-5 39 0.15144454799627213 4.017096763826847E-5 4.017096763826847E-5 40 0.1744223414853617 4.017096763826847E-5 4.017096763826847E-5 41 0.19808304142430183 4.017096763826847E-5 4.017096763826847E-5 42 0.22419417038917633 4.017096763826847E-5 4.017096763826847E-5 43 0.25287624128290004 4.017096763826847E-5 4.017096763826847E-5 44 0.28453096378185555 4.017096763826847E-5 4.017096763826847E-5 45 0.3189173120802134 4.017096763826847E-5 4.017096763826847E-5 46 0.35539255069576114 4.017096763826847E-5 4.017096763826847E-5 47 0.3934344570492014 4.017096763826847E-5 4.017096763826847E-5 48 0.43593534081048946 8.034193527653694E-5 4.017096763826847E-5 49 0.4801234052125848 8.034193527653694E-5 4.017096763826847E-5 50 0.5292123276665488 8.034193527653694E-5 4.017096763826847E-5 51 0.5800286017289584 8.034193527653694E-5 4.017096763826847E-5 52 0.632331201593984 8.034193527653694E-5 4.017096763826847E-5 53 0.6897355143490697 8.034193527653694E-5 4.017096763826847E-5 54 0.7501928206446636 8.034193527653694E-5 4.017096763826847E-5 55 0.8167962849889128 1.2051290291480541E-4 4.017096763826847E-5 56 0.8855288106179902 1.2051290291480541E-4 4.017096763826847E-5 57 0.9577562104315969 1.2051290291480541E-4 4.017096763826847E-5 58 1.0377366069993894 1.2051290291480541E-4 4.017096763826847E-5 59 1.1186007648552239 1.2051290291480541E-4 4.017096763826847E-5 60 1.2096683484911785 1.2051290291480541E-4 4.017096763826847E-5 61 1.3045923450204069 1.2051290291480541E-4 4.017096763826847E-5 62 1.4065060899186939 1.2051290291480541E-4 4.017096763826847E-5 63 1.518301892855995 1.2051290291480541E-4 4.017096763826847E-5 64 1.6335523990101875 1.2051290291480541E-4 4.017096763826847E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGTGT 1975 0.0 25.164557 2 GTGTTTG 2355 0.0 21.252653 5 TGTGTTT 2695 0.0 18.83117 4 CTTTGTG 2830 0.0 18.303886 1 TTGTGTT 4025 0.0 13.217391 3 GTTTGAC 880 0.0 11.931819 2 TGTTTGA 4775 0.0 11.581152 1 TTGAGGG 660 3.6379788E-12 11.136363 9 AGGGGCA 320 0.008878235 8.750001 12 GCGGTGT 500 6.639551E-4 7.7000003 11 GTCACGT 735 1.9126583E-6 7.619047 70 TTTGAGT 1320 2.0008883E-11 7.1590905 3 CACGTTT 685 4.761826E-5 7.1532845 70 CCTGTCC 490 0.0046303123 7.142857 11 GTTTGAG 4865 0.0 7.0503592 7 CTCCCAG 665 2.564949E-4 6.8421054 1 CGTTTCG 520 0.008236039 6.730769 66 AGTCACG 845 1.5254203E-5 6.6272182 69 CTGAACT 2275 0.0 6.461538 70 TTTGACG 705 5.2279147E-4 6.4539013 3 >>END_MODULE