FastQCFastQC Report
Mon 15 Sep 2014
pBOB11_1_GTTTCG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamepBOB11_1_GTTTCG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2489360
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTGT19750.025.1645572
GTGTTTG23550.021.2526535
TGTGTTT26950.018.831174
CTTTGTG28300.018.3038861
TTGTGTT40250.013.2173913
GTTTGAC8800.011.9318192
TGTTTGA47750.011.5811521
TTGAGGG6603.6379788E-1211.1363639
AGGGGCA3200.0088782358.75000112
GCGGTGT5006.639551E-47.700000311
GTCACGT7351.9126583E-67.61904770
TTTGAGT13202.0008883E-117.15909053
CACGTTT6854.761826E-57.153284570
CCTGTCC4900.00463031237.14285711
GTTTGAG48650.07.05035927
CTCCCAG6652.564949E-46.84210541
CGTTTCG5200.0082360396.73076966
AGTCACG8451.5254203E-56.627218269
CTGAACT22750.06.46153870
TTTGACG7055.2279147E-46.45390133
Produced by FastQC (version 0.11.2)