##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename lane1_Undetermined_L001_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 490122 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.4476783331497 34.0 34.0 34.0 31.0 34.0 2 32.601899118994865 34.0 34.0 34.0 31.0 34.0 3 32.61247607738481 34.0 34.0 34.0 32.0 34.0 4 32.64159127727382 34.0 34.0 34.0 32.0 34.0 5 32.6849825145576 34.0 34.0 34.0 32.0 34.0 6 36.12007010499427 38.0 38.0 38.0 34.0 38.0 7 35.84766037843639 38.0 37.0 38.0 31.0 38.0 8 35.91741852028679 38.0 37.0 38.0 32.0 38.0 9 35.99268549463195 38.0 38.0 38.0 32.0 38.0 10-11 36.14418654947136 38.0 38.0 38.0 34.0 38.0 12-13 35.990720677708815 38.0 38.0 38.0 34.0 38.0 14-15 35.83773631871249 38.0 37.0 38.0 31.0 38.0 16-17 35.899393416333076 38.0 37.0 38.0 31.5 38.0 18-19 36.054040626619496 38.0 37.0 38.0 34.0 38.0 20-21 36.04828389666246 38.0 37.5 38.0 34.0 38.0 22-23 36.13690876965327 38.0 38.0 38.0 34.0 38.0 24-25 35.97864307254112 38.0 38.0 38.0 33.0 38.0 26-27 35.94581655179731 38.0 38.0 38.0 33.0 38.0 28-29 35.8355797128062 38.0 37.5 38.0 32.5 38.0 30-31 35.92001583279265 38.0 37.5 38.0 34.0 38.0 32-33 35.928930960046685 38.0 38.0 38.0 33.5 38.0 34-35 35.927771044760284 38.0 38.0 38.0 32.5 38.0 36-37 35.71717858002701 38.0 37.0 38.0 31.0 38.0 38-39 35.7764474967457 38.0 37.0 38.0 31.5 38.0 40-41 35.89900779805844 38.0 37.5 38.0 32.5 38.0 42-43 35.749664369279486 38.0 37.0 38.0 31.0 38.0 44-45 35.664288687306424 38.0 37.5 38.0 31.0 38.0 46-47 35.535113706383314 38.0 37.0 38.0 31.0 38.0 48-49 35.38408600307679 38.0 37.0 38.0 29.0 38.0 50-51 35.50481818812459 38.0 37.0 38.0 31.0 38.0 52-53 35.43558746597786 38.0 37.0 38.0 28.0 38.0 54-55 35.583342514720826 38.0 37.0 38.0 31.0 38.0 56-57 35.589583817906565 38.0 37.0 38.0 31.0 38.0 58-59 35.67077380733777 38.0 37.0 38.0 31.0 38.0 60-61 35.70327285859439 38.0 37.0 38.0 31.0 38.0 62-63 35.74655595953661 38.0 37.0 38.0 31.0 38.0 64-65 35.56345562941472 38.0 37.0 38.0 31.0 38.0 66-67 35.420556514500475 38.0 37.0 38.0 28.0 38.0 68-69 30.824220296171156 38.0 24.0 38.0 11.0 38.0 70-71 30.05072920619764 38.0 21.5 38.0 11.0 38.0 72-73 30.200423568009597 38.0 22.0 38.0 11.0 38.0 74-75 30.269180938623443 38.0 22.0 38.0 11.0 38.0 76 29.925314513529283 37.0 21.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3023502389898738 1101 2 0.44492605012786157 1101 3 0.39753810338790174 1101 4 0.3582396897066147 1101 5 0.335923413954788 1101 6 0.6672454711908387 1101 7 0.49979762507175707 1101 8 0.6714086561997945 1101 9 0.40504359400925694 1101 10-11 0.5899018715986912 1101 12-13 0.5140242951324012 1101 14-15 0.6352415274075938 1101 16-17 0.3803005962831847 1101 18-19 0.3730934070113392 1101 20-21 0.5101584339505507 1101 22-23 0.5562117241500886 1101 24-25 0.5380837817455273 1101 26-27 0.4597225161084708 1101 28-29 0.5037727419599705 1101 30-31 0.41962515167021763 1101 32-33 0.5716080774867223 1101 34-35 0.5324265099246119 1101 36-37 0.6684633782335396 1101 38-39 0.8072667688912603 1101 40-41 0.65968218123637 1101 42-43 0.5033958139472574 1101 44-45 0.665849098873359 1101 46-47 0.6484690325148108 1101 48-49 0.7935483275868478 1101 50-51 0.5566249379478379 1101 52-53 0.5345093917998085 1101 54-55 0.6423489980927997 1101 56-57 0.5702581290842872 1101 58-59 0.5059617109319845 1101 60-61 0.7701697957681617 1101 62-63 0.6912409138740045 1101 64-65 0.8786824980061212 1101 66-67 0.6478309532955464 1101 68-69 0.21147058860821488 1101 70-71 -0.010599046641026177 1101 72-73 0.15318370337096354 1101 74-75 0.10553515921777645 1101 76 -0.1134304305149918 1102 1 0.3053030341696328 1102 2 0.20027149209794004 1102 3 0.2668188764833701 1102 4 0.399521991194014 1102 5 0.42476603190637974 1102 6 0.6400016431010727 1102 7 0.375614571117346 1102 8 0.6972208260205832 1102 9 0.5999069153838903 1102 10-11 0.6639430293555719 1102 12-13 0.4792346364450495 1102 14-15 0.6280654007928916 1102 16-17 0.10756062726213855 1102 18-19 0.33839473842935064 1102 20-21 0.3154795111282951 1102 22-23 0.4849552868268603 1102 24-25 0.403104645988293 1102 26-27 0.3515776018205443 1102 28-29 0.5980406071567756 1102 30-31 0.4440630988367076 1102 32-33 0.6024818364240048 1102 34-35 0.478139576103743 1102 36-37 0.3704620167071795 1102 38-39 0.22357068430734017 1102 40-41 0.15049429664447445 1102 42-43 0.23225981003921703 1102 44-45 0.48430963093271373 1102 46-47 0.27557253632914325 1102 48-49 0.1588865991738686 1102 50-51 0.12973140664905713 1102 52-53 0.20796101831594882 1102 54-55 0.1947976899513293 1102 56-57 0.47885025520563573 1102 58-59 0.5072313403090334 1102 60-61 0.6352586758704106 1102 62-63 0.5476790835279246 1102 64-65 0.8739355955995123 1102 66-67 0.5701181482973183 1102 68-69 0.63641040212989 1102 70-71 0.3745551165946175 1102 72-73 0.4829490758620878 1102 74-75 0.3617560408725957 1102 76 0.6218578902248275 1103 1 0.12675689303203796 1103 2 0.29998112083754336 1103 3 0.23727038233727882 1103 4 0.15596773253472662 1103 5 0.08941583415353449 1103 6 -0.035501321403543784 1103 7 0.11974456589638294 1103 8 0.3667518692830356 1103 9 0.13760869122585717 1103 10-11 0.07192895421665213 1103 12-13 0.20982052840691523 1103 14-15 0.12516884019040475 1103 16-17 0.19919564632222375 1103 18-19 0.07358473134886623 1103 20-21 0.3068345293301391 1103 22-23 0.1200297749223509 1103 24-25 0.19968563167713427 1103 26-27 0.19344863692972325 1103 28-29 0.2535769715083376 1103 30-31 0.1837534886923109 1103 32-33 0.260557161303673 1103 34-35 0.30334108374565716 1103 36-37 0.3288713400563168 1103 38-39 0.16406960793111836 1103 40-41 0.21429193791438905 1103 42-43 0.36842497836157406 1103 44-45 0.2357221512265113 1103 46-47 0.31570062867579196 1103 48-49 0.2858382015798 1103 50-51 0.2969165934235747 1103 52-53 0.33508248075721525 1103 54-55 0.20132746730952533 1103 56-57 0.37817331826483525 1103 58-59 0.4482952720503306 1103 60-61 0.5350644990300211 1103 62-63 0.34069479647519785 1103 64-65 0.6738601419838162 1103 66-67 0.4078928745544559 1103 68-69 0.42185214550177363 1103 70-71 0.23354625526477335 1103 72-73 0.36470503526732756 1103 74-75 0.4463570664696128 1103 76 0.1735883169847554 2116 1 0.06606858440886754 2116 2 0.36135602160351965 2116 3 0.15517613326167634 2116 4 0.28867669613818947 2116 5 0.26208660606905454 2116 6 0.2644035569766743 2116 7 -0.15947314818090064 2116 8 0.3925956266585402 2116 9 0.22111903659600785 2116 10-11 0.12805786189146318 2116 12-13 0.27508790598253086 2116 14-15 0.10892664481203695 2116 16-17 0.20245695900057825 2116 18-19 0.11510925198596311 2116 20-21 0.1703439449401003 2116 22-23 0.006486387305329799 2116 24-25 -0.003195194522078282 2116 26-27 -0.08343037266111963 2116 28-29 0.0641490601073258 2116 30-31 0.12229276390848298 2116 32-33 0.1068678013362856 2116 34-35 0.23850640226003605 2116 36-37 0.221737933772566 2116 38-39 0.01264564443493299 2116 40-41 0.16763943221066313 2116 42-43 0.03529011306952157 2116 44-45 -0.14368079968929948 2116 46-47 0.037534258817181865 2116 48-49 0.23982758800958948 2116 50-51 0.07909344811636032 2116 52-53 0.1860311937545518 2116 54-55 -0.037259726316612785 2116 56-57 0.2224706698786818 2116 58-59 0.06743867683215399 2116 60-61 0.2648440028241552 2116 62-63 0.022958190385978128 2116 64-65 0.31332953499344995 2116 66-67 0.009910811774197725 2116 68-69 0.831785202767847 2116 70-71 0.9279841437078566 2116 72-73 0.9366970822416718 2116 74-75 0.8270670456572873 2116 76 0.8491306174975541 1117 1 0.1441950865571684 1117 2 0.2534515477590844 1117 3 0.255544906818443 1117 4 0.4900218814986701 1117 5 0.1618682014090993 1117 6 0.07666059411779003 1117 7 -0.8166111104111877 1117 8 0.1969016002478483 1117 9 0.1716464370135924 1117 10-11 0.2342805459023154 1117 12-13 0.2221962627955989 1117 14-15 0.06464978362717488 1117 16-17 0.39640572018272024 1117 18-19 0.1522183236675616 1117 20-21 0.37624826595057925 1117 22-23 0.16022564270812722 1117 24-25 0.31876864792089776 1117 26-27 0.20591389569560192 1117 28-29 0.4736966222824037 1117 30-31 0.22516111224172874 1117 32-33 0.24852392985485494 1117 34-35 0.1847045342346334 1117 36-37 0.4890870533750018 1117 38-39 0.15640632124286924 1117 40-41 0.5059484969900794 1117 42-43 0.15361441194372816 1117 44-45 0.35344555209010764 1117 46-47 0.2424494729449549 1117 48-49 0.43087866659828933 1117 50-51 0.3519452065696811 1117 52-53 0.3984697828505901 1117 54-55 0.07837642039412884 1117 56-57 0.45213142778722215 1117 58-59 -0.08948112724456792 1117 60-61 0.1973548675932122 1117 62-63 -0.44781108209971876 1117 64-65 0.2964530913904113 1117 66-67 -0.42993978070391137 1117 68-69 0.6041630526898274 1117 70-71 -0.16433126614598947 1117 72-73 0.20817888144249963 1117 74-75 0.07423715232060246 1117 76 0.25081031202683945 2117 1 0.08572877234469445 2117 2 0.17461789672668715 2117 3 0.08635384730888518 2117 4 0.15923488537392672 2117 5 -0.026429283529353143 2117 6 -0.5169285670262127 2117 7 -0.019401959254842893 2117 8 0.268455652779096 2117 9 -0.1669058991124075 2117 10-11 0.04378417621212094 2117 12-13 0.030601192093222096 2117 14-15 0.014473101828777146 2117 16-17 0.2556326142483769 2117 18-19 0.013170222804141929 2117 20-21 0.25226200668205223 2117 22-23 0.0390361038838023 2117 24-25 0.2294206802657115 2117 26-27 0.14823046155657238 2117 28-29 0.025531882963676367 2117 30-31 0.2950761578919412 2117 32-33 0.15243281447612844 2117 34-35 0.06868941753211999 2117 36-37 0.41279776258132017 2117 38-39 0.1691266397089919 2117 40-41 0.08897144605186469 2117 42-43 -0.01698405242515122 2117 44-45 0.2434698806814808 2117 46-47 0.03243744506018942 2117 48-49 0.3095663579442629 2117 50-51 0.2541786092155789 2117 52-53 0.36491417879336296 2117 54-55 -0.4574050540537371 2117 56-57 0.04891675614017288 2117 58-59 -0.20068597967760127 2117 60-61 -0.5239840115360295 2117 62-63 -0.7567539449023641 2117 64-65 -0.18058005409124434 2117 66-67 -0.5702247727742886 2117 68-69 -0.8720350207134828 2117 70-71 -0.7174562031300766 2117 72-73 -0.565431180227101 2117 74-75 -0.6571507635696108 2117 76 -0.46623962979093747 1116 1 0.3857911396793057 1116 2 0.38940117173667943 1116 3 0.45286708194485925 1116 4 0.33581951592984893 1116 5 0.4176207469353841 1116 6 0.5802434547837834 1116 7 0.8105095582125088 1116 8 0.6458517177058312 1116 9 0.5624642027901814 1116 10-11 0.5255736334365722 1116 12-13 0.6682391895565942 1116 14-15 0.7530826757238813 1116 16-17 0.7707274862874982 1116 18-19 0.611177104179788 1116 20-21 0.7483276523457292 1116 22-23 0.5360890825680684 1116 24-25 0.5634963106940205 1116 26-27 0.633453117734021 1116 28-29 0.6384518065412266 1116 30-31 0.6094710299909778 1116 32-33 0.6035145971634108 1116 34-35 0.44384930497135855 1116 36-37 0.6979312113032066 1116 38-39 0.6489495188751278 1116 40-41 0.6365473577622467 1116 42-43 0.6229757952332022 1116 44-45 0.7583703415766792 1116 46-47 0.6738306630169504 1116 48-49 0.9134798632580754 1116 50-51 0.6432600120672163 1116 52-53 0.7149671700589479 1116 54-55 0.607746044444113 1116 56-57 0.8509130500466853 1116 58-59 0.6194007354810083 1116 60-61 0.8444412821625988 1116 62-63 0.6940210935455156 1116 64-65 0.8951576148801976 1116 66-67 0.6627211162238424 1116 68-69 0.799697718782177 1116 70-71 0.2615845381365496 1116 72-73 0.6925817641401579 1116 74-75 0.7073779197127656 1116 76 0.8201666277915471 2118 1 -0.26867982275159363 2118 2 -0.3818039651859877 2118 3 -0.2670723767384544 2118 4 0.10084856414142251 2118 5 -0.2592529187999233 2118 6 -0.27052196947428087 2118 7 0.0797372077148708 2118 8 0.04778186048186228 2118 9 -0.6212318664538046 2118 10-11 -0.26020537004783506 2118 12-13 -0.06338858604474495 2118 14-15 -0.1867588491533354 2118 16-17 -0.31655780172383885 2118 18-19 -0.10036659009461601 2118 20-21 -0.10929239048881101 2118 22-23 -0.2578803142680641 2118 24-25 -0.3086763137467514 2118 26-27 -0.31813223752626385 2118 28-29 -0.18673859313847174 2118 30-31 -0.2500082824704464 2118 32-33 -0.18953580733703745 2118 34-35 -0.11719220817037268 2118 36-37 -0.10327449513583531 2118 38-39 0.016776273143776166 2118 40-41 -0.0992450782548886 2118 42-43 -0.0752202184973143 2118 44-45 -0.19157146995711827 2118 46-47 0.029833624502110467 2118 48-49 0.03663432818018464 2118 50-51 -0.1099038239879988 2118 52-53 0.09886722971435091 2118 54-55 -0.05637879584681116 2118 56-57 -0.08671972924501148 2118 58-59 -0.028169011578931702 2118 60-61 -0.1687703472467703 2118 62-63 -0.07262158440102695 2118 64-65 -0.2827800109958645 2118 66-67 -0.278900291148922 2118 68-69 0.37026281134009054 2118 70-71 0.4144804138600229 2118 72-73 0.35585952072885974 2118 74-75 0.4106489571587595 2118 76 0.15632798386181435 1119 1 0.17869714937891956 1119 2 0.262575426594168 1119 3 0.41214532790181835 1119 4 0.10790210396699962 1119 5 0.3702180657131535 1119 6 0.46080059825560227 1119 7 0.6854602303620965 1119 8 0.2992954470757212 1119 9 0.27796390467170795 1119 10-11 0.18552310696549767 1119 12-13 0.050854272507287135 1119 14-15 0.47804656343463847 1119 16-17 0.3904789108020523 1119 18-19 0.47031213033430674 1119 20-21 0.3518211705739773 1119 22-23 0.3680418507671277 1119 24-25 0.26597684079168715 1119 26-27 0.29654174804867495 1119 28-29 0.17059143662844178 1119 30-31 0.47587123406383114 1119 32-33 0.3305852911955043 1119 34-35 0.21287817636243744 1119 36-37 0.24715518125778146 1119 38-39 0.4565122005891027 1119 40-41 0.40780381955698175 1119 42-43 0.34674259897290227 1119 44-45 0.23373069612252806 1119 46-47 0.4221516720711449 1119 48-49 0.12092053153519089 1119 50-51 0.2650998025852118 1119 52-53 0.45182506282235124 1119 54-55 0.5433333993034495 1119 56-57 0.39284264491289633 1119 58-59 0.39521687321789045 1119 60-61 0.3371807143237362 1119 62-63 0.6465249813792084 1119 64-65 0.5245616238053117 1119 66-67 0.5680472947138284 1119 68-69 0.24704723042108867 1119 70-71 0.21776173729812953 1119 72-73 -0.10596513880844682 1119 74-75 0.04103180723912203 1119 76 0.08165771189783655 2119 1 0.20517967890647526 2119 2 0.40033379273589986 2119 3 0.28569970782307763 2119 4 0.24281844254967666 2119 5 0.15439621531945846 2119 6 0.181543708491823 2119 7 0.2680512736691796 2119 8 -0.3529498777274256 2119 9 -0.47306955989521526 2119 10-11 0.07725231464265647 2119 12-13 -0.14689425111475884 2119 14-15 0.13594518548188717 2119 16-17 -0.2236912417570025 2119 18-19 0.22457209588548466 2119 20-21 0.032520604629091565 2119 22-23 0.33229504958602973 2119 24-25 0.28225698350822626 2119 26-27 0.2971692086786035 2119 28-29 -0.21120605352903254 2119 30-31 0.22469320748902533 2119 32-33 0.12459980891204481 2119 34-35 0.05934409663802853 2119 36-37 -0.5857680470886777 2119 38-39 -0.02888396082822453 2119 40-41 -0.061109197175305496 2119 42-43 0.3583936816500639 2119 44-45 -0.07844019462550023 2119 46-47 0.13138518585066805 2119 48-49 0.0043143553540900825 2119 50-51 0.4011603340812684 2119 52-53 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30-31 -1.0025126066801135 2111 32-33 -1.256847242274901 2111 34-35 -0.9874068770266575 2111 36-37 -1.0520328838839248 2111 38-39 -1.3002350364958772 2111 40-41 -1.1779733014037888 2111 42-43 -1.1421858806644778 2111 44-45 -0.9891284828302531 2111 46-47 -1.092026813407486 2111 48-49 -1.1169103757289918 2111 50-51 -1.2243450442570065 2111 52-53 -1.1385018139738392 2111 54-55 -1.3212820122715883 2111 56-57 -1.1556818533230384 2111 58-59 -1.1847330087886974 2111 60-61 -0.8723241806504447 2111 62-63 -0.6846918694817887 2111 64-65 -0.6560284600751558 2111 66-67 -0.8092039261090918 2111 68-69 -0.7329412038881244 2111 70-71 -0.8203629852421166 2111 72-73 -1.0971500964009167 2111 74-75 -0.8601031491850932 2111 76 -0.49837102709139103 2110 1 0.20359606404161212 2110 2 0.13173532294257484 2110 3 0.22519816767999856 2110 4 0.18164495288353777 2110 5 0.17490992756111012 2110 6 0.2871428738336377 2110 7 0.4560008960857118 2110 8 0.5363771791088254 2110 9 0.5067297246834812 2110 10-11 0.33194913002422055 2110 12-13 0.5803787019854028 2110 14-15 0.42237702172990055 2110 16-17 0.27972005096955144 2110 18-19 0.13768629004288613 2110 20-21 0.27467146412829635 2110 22-23 0.5355355603173493 2110 24-25 0.21000998927134873 2110 26-27 0.403565770681773 2110 28-29 0.2295270048970366 2110 30-31 0.5086291425049225 2110 32-33 0.5564149420598952 2110 34-35 0.3931073865823862 2110 36-37 0.5746353710353276 2110 38-39 0.5786652243863983 2110 40-41 0.35156406892167524 2110 42-43 0.15731806559282546 2110 44-45 0.3770490492139231 2110 46-47 0.46747545810822544 2110 48-49 0.48753983726983563 2110 50-51 0.36749330307968364 2110 52-53 0.38487026643263533 2110 54-55 0.4400561062623751 2110 56-57 0.5940669354378016 2110 58-59 0.545733253423947 2110 60-61 0.31310863390612553 2110 62-63 0.3989378852511223 2110 64-65 0.5963710200473216 2110 66-67 0.5745636999564923 2110 68-69 0.21779131865012502 2110 70-71 0.31968079391843673 2110 72-73 0.39939958209587445 2110 74-75 0.5277298531312375 2110 76 0.38854027765025023 2109 1 0.10050726095280993 2109 2 0.03580073289033692 2109 3 0.27498240569952515 2109 4 0.41966448502071785 2109 5 0.28942275972099907 2109 6 0.38387237644548833 2109 7 0.2327894493448568 2109 8 0.4516622120409153 2109 9 0.46498545940980307 2109 10-11 0.3563983691793382 2109 12-13 0.325529735371731 2109 14-15 0.3786454750571764 2109 16-17 0.5058499623935759 2109 18-19 0.5126919679897313 2109 20-21 0.2736267219070925 2109 22-23 0.35461346174818686 2109 24-25 0.23709379576691703 2109 26-27 0.1830343148562079 2109 28-29 0.2599721559304271 2109 30-31 0.35828846569366135 2109 32-33 0.5078798523483385 2109 34-35 0.41244647356852937 2109 36-37 0.760781180710552 2109 38-39 0.38352554689378593 2109 40-41 0.4589453998324231 2109 42-43 0.32476071832959974 2109 44-45 0.44303814755596704 2109 46-47 0.43222676403600246 2109 48-49 0.5156002506243595 2109 50-51 0.18026868350212055 2109 52-53 0.21561067070244633 2109 54-55 0.39140745761373097 2109 56-57 0.27569309941690534 2109 58-59 0.24407529294245478 2109 60-61 0.11019016922295322 2109 62-63 0.3511977080991784 2109 64-65 0.11201944157809152 2109 66-67 0.2747113265747103 2109 68-69 -0.1691948271595507 2109 70-71 -0.019519751164462917 2109 72-73 -0.08873350897839316 2109 74-75 -0.032626721931219294 2109 76 0.08304402509517317 2108 1 0.1456214227336332 2108 2 0.27604752730379545 2108 3 0.4417664516502384 2108 4 0.44432307217930145 2108 5 0.2400829437145191 2108 6 0.13042488133132935 2108 7 0.08766932922473814 2108 8 0.34260374881578315 2108 9 0.41142118084552237 2108 10-11 -0.0012247301104295616 2108 12-13 0.4279321377741283 2108 14-15 0.2581288274571989 2108 16-17 0.4364987659590511 2108 18-19 0.31628216532993036 2108 20-21 0.17077774197478135 2108 22-23 0.2531766082280029 2108 24-25 0.26009745418723895 2108 26-27 -0.05547353823498469 2108 28-29 0.15190158535985177 2108 30-31 0.05139556088408881 2108 32-33 0.3073447934799418 2108 34-35 0.2065807864528466 2108 36-37 0.7075961504421855 2108 38-39 0.18591156386312946 2108 40-41 0.32135125807160847 2108 42-43 0.2653188359710512 2108 44-45 0.42099556926338266 2108 46-47 0.10627909017166104 2108 48-49 0.3274287457861931 2108 50-51 0.3641811674145998 2108 52-53 0.09514794607305532 2108 54-55 0.21012826175120125 2108 56-57 0.3186628548866608 2108 58-59 0.00497190383906343 2108 60-61 -0.005003595970812569 2108 62-63 0.30020802794283696 2108 64-65 0.16436732517597363 2108 66-67 -0.022882398102346713 2108 68-69 -0.4074086362066929 2108 70-71 8.398941951845984E-4 2108 72-73 -0.12322782472270788 2108 74-75 -0.16247383094499313 2108 76 -0.14233373361592072 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 4.0 10 6.0 11 55.0 12 87.0 13 313.0 14 1065.0 15 2878.0 16 3851.0 17 4041.0 18 3794.0 19 3452.0 20 2875.0 21 2347.0 22 1982.0 23 2060.0 24 2184.0 25 2652.0 26 3138.0 27 3571.0 28 4411.0 29 5047.0 30 6083.0 31 7695.0 32 10396.0 33 15821.0 34 50136.0 35 76109.0 36 29031.0 37 245030.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.96202198447987 17.771434051661693 16.865446576326384 21.40109738753206 2 16.693259119306937 49.431560820543794 18.696874604683806 15.178305455465463 3 15.885691900251778 19.27918810725668 48.38527363021664 16.4498463622749 4 16.269072093431326 18.78950611490388 18.17189470204891 46.769527089615885 5 46.8419592176514 18.793994866500448 19.570956961033556 14.793088954814596 6 45.84892494399262 18.91355888629992 19.020880856290578 16.216635313416877 7 15.742427797557715 47.70411025983698 21.003233934892833 15.550228007712475 8 15.137906691096356 47.96781973173589 20.612755399764136 16.281518177403626 9 45.50972730889689 17.19922875243565 21.493322995623476 15.79772094304398 10-11 30.617743404857436 33.82138255448472 19.533598781509216 16.027275259148627 12-13 30.68772705153592 18.18860694796828 20.314334711582703 30.8093312889131 14-15 15.815504148014545 18.350322578012463 34.91432605475461 30.91984721921839 16-17 31.098887197672376 18.096810129888148 35.037766723660205 15.766535948779264 18-19 30.9165826726027 17.961229428255468 35.22537613698037 15.896811762161455 20-21 30.73662454041305 32.95886263195909 20.063903500002038 16.24060932762582 22-23 46.069689908878345 18.159346600994873 19.921283619729287 15.849679870397498 24-25 15.758476599321792 48.06606218145166 19.668587308258896 16.506873910967652 26-27 30.891313950748145 33.024289271770726 19.522416746325085 16.561980031156047 28-29 30.996454895559694 33.00303501747048 19.525517100665663 16.474992986304166 30-31 31.033589226963198 17.956693616261575 34.665000382565225 16.344716774210003 32-33 16.09752862863118 33.21737356219236 34.3301792955699 16.35491851360657 34-35 30.87944845484928 33.29715127500949 19.497096593853673 16.32630367628756 36-37 16.22347045789347 18.18923760594474 34.33833761124963 31.248954324912166 38-39 16.292536022231562 18.372358267668336 34.17133564844097 31.163770061659125 40-41 45.986137918933146 18.180362119833347 19.53178240164533 16.301717559588177 42-43 31.20314865521083 18.039782581195396 34.589401649412174 16.1676671141816 44-45 31.24762810284954 18.056921450927742 34.410973773448625 16.28447667277409 46-47 31.11551598199603 18.33828456236712 19.21134674789947 31.334852707737383 48-49 16.11188020733973 18.627726026726464 18.97621673347092 46.284177032462885 50-51 31.295780165430898 18.279216672039567 34.221324018493654 16.203679144035878 52-53 16.227857192408297 33.31755469135752 34.086967521841856 16.367620594392328 54-55 15.811423464744937 33.41742941438115 19.350191996147835 31.420955124726085 56-57 16.26101274397385 18.34185516022803 49.23946265562706 16.157669440171063 58-59 15.920071656798216 48.452604904165156 19.19879864684543 16.428524792191205 60-61 15.916807110182528 48.584615007936925 18.962731119698685 16.535846762181862 62-63 15.882631387799574 48.40883957609862 19.233076386310124 16.47545264979168 64-65 15.88048902908303 48.434853931942364 19.118103135188953 16.56655390378565 66-67 15.70981445133173 48.2602006880032 19.350702081556538 16.679282779108533 68-69 17.334538495125624 40.87395993585166 22.350004284717436 19.441497284305285 70-71 18.630971569879662 36.78970606838409 23.98472600252186 20.594596359214385 72-73 18.399290777247742 35.969896799520114 24.638349445231107 20.992462978001036 74-75 18.990479765932008 34.64112430985444 24.827795166022597 21.54060075819095 76 17.942152233970056 35.451343973034845 25.112320806995925 21.494182985999174 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 585.0 1 361.5 2 132.5 3 135.0 4 143.0 5 134.5 6 104.5 7 85.0 8 87.0 9 86.0 10 103.5 11 119.5 12 117.0 13 122.5 14 145.5 15 163.0 16 193.0 17 223.0 18 237.5 19 292.0 20 348.0 21 364.0 22 400.5 23 496.0 24 610.5 25 666.0 26 746.0 27 956.0 28 1199.5 29 1313.0 30 1564.0 31 2223.0 32 2736.5 33 2842.0 34 3477.0 35 5239.0 36 7819.5 37 9273.0 38 10828.0 39 27250.0 40 50498.5 41 58308.0 42 57736.0 43 53041.5 44 45127.0 45 40132.0 46 38357.0 47 35532.0 48 28433.0 49 22307.0 50 20455.0 51 18035.5 52 13440.5 53 10497.5 54 9730.0 55 8755.0 56 6940.5 57 5218.5 58 4336.0 59 4182.5 60 3817.0 61 3210.0 62 2815.0 63 2636.0 64 2349.5 65 2048.0 66 1578.0 67 1302.0 68 999.5 69 732.0 70 835.5 71 904.0 72 829.5 73 597.5 74 332.0 75 224.0 76 183.5 77 101.5 78 58.5 79 57.0 80 43.5 81 32.5 82 27.5 83 20.0 84 20.5 85 24.5 86 22.5 87 17.0 88 15.5 89 12.5 90 12.0 91 12.0 92 11.0 93 18.0 94 21.0 95 16.0 96 15.0 97 21.0 98 37.0 99 225.5 100 404.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009997510823835697 2 0.0016322466651160325 3 0.0016322466651160325 4 0.0016322466651160325 5 0.0016322466651160325 6 0.0016322466651160325 7 0.0014282158319765283 8 0.0016322466651160325 9 0.0014282158319765283 10-11 0.0015302312485462803 12-13 0.0015302312485462803 14-15 0.0016322466651160325 16-17 0.0016322466651160325 18-19 0.0016322466651160325 20-21 0.0016322466651160325 22-23 0.0016322466651160325 24-25 0.0016322466651160325 26-27 0.0017342620816857843 28-29 0.0019382929148252883 30-31 0.0019382929148252883 32-33 0.0019382929148252883 34-35 0.0016322466651160325 36-37 0.0016322466651160325 38-39 0.0016322466651160325 40-41 0.0016322466651160325 42-43 0.0016322466651160325 44-45 0.0016322466651160325 46-47 0.0016322466651160325 48-49 0.0017342620816857843 50-51 0.0016322466651160325 52-53 0.0016322466651160325 54-55 0.0016322466651160325 56-57 0.0016322466651160325 58-59 0.0016322466651160325 60-61 0.0016322466651160325 62-63 0.0016322466651160325 64-65 0.0016322466651160325 66-67 0.0016322466651160325 68-69 0.0016322466651160325 70-71 0.0016322466651160325 72-73 0.0016322466651160325 74-75 0.0016322466651160325 76 0.0016322466651160325 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 490122.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.8112739336802 #Duplication Level Percentage of deduplicated Percentage of total 1 86.20057647257948 28.283507278829532 2 2.8299769183209973 1.857102957860447 3 1.4795144586647104 1.4563426257626513 4 0.9760235513926558 1.280983044418713 5 0.6658359857824797 1.0923463462205467 6 0.5223359647363949 1.0283105054627384 7 0.5180818038250083 1.189924678975026 8 0.4218490647273013 1.1073124177147415 9 0.5679264668552236 1.6770951790356499 >10 5.780912217811869 32.486755966511524 >50 0.025527270638509285 0.5549528647046765 >100 0.008940430925174455 0.5480619640969573 >500 0.0012496971150988709 0.28825918053543537 >1k 6.248483125649449E-4 0.3128615752376407 >5k 0.0 0.0 >10k+ 6.248483125649449E-4 26.83618341463367 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 130895 26.706615903795385 Illumina Single End PCR Primer 1 (100% over 50bp) CATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 1526 0.3113510513708832 Illumina Single End PCR Primer 1 (98% over 50bp) GCTCTTCCTCTCTTCTTTTCTTTCCCTCTTTTCTCTCTCTTTTTTCTCCT 857 0.17485442400055495 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 549 0.11201292739358772 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.008365264158719665 0.0 0.0 2 0.00856929499185917 0.0 0.0 3 0.009997510823835699 0.0 0.0 4 0.011221695822672722 0.0 0.0 5 0.012649911654649251 0.0 0.0 6 0.013466034987207267 0.0 0.0 7 0.014078127486625779 0.0 0.0 8 0.015710374151741813 0.0 0.0 9 0.016934559150578836 0.0 0.0 10 0.020403083313950403 0.0 0.0 11 0.023463545811042964 0.0 0.0 12 0.024075638310461476 0.0 0.0 13 0.02734013164069354 0.0 0.0 14 0.02917640913894908 0.0 0.0 15 0.030604624970925608 0.0 0.0 16 0.03366508746801817 0.0 0.0 17 0.03468524163371569 0.0 0.0 18 0.0355013649662737 0.0 0.0 19 0.037949734963947754 0.0 0.0 20 0.038969889129645274 0.0 0.0 21 0.03958198162906378 0.0 0.0 22 0.039786012462203285 0.0 0.0 23 0.040806166627900806 0.0 0.0 24 0.04162228996045882 0.0 0.0 25 0.04284647495929585 0.0 0.0 26 0.04407065995813287 0.0 0.0 27 0.045702906623248905 0.0 0.0 28 0.047539184121504444 0.0 0.0 29 0.052435924116852536 0.0 0.0 30 0.06447374327208327 0.0 0.0 31 0.06814629826859435 0.0 0.0 32 0.0705946682662684 0.0 0.0 33 0.07283900743080295 0.0 0.0 34 0.07447125409591898 0.0 0.0 35 0.07630753159417451 0.0 0.0 36 0.07936799409126707 0.0 0.0 37 0.08100024075638311 0.0 0.0 38 0.08508085741917319 0.0 0.0 39 0.08814131991626574 0.0 0.0 40 0.09222193657905582 0.0 0.0 41 0.09691464574126442 0.0 0.0 42 0.1014033240703335 0.0 0.0 43 0.10670812573196062 0.0 0.0 44 0.1111968040610297 0.0 0.0 45 0.11833788322091235 0.0 0.0 46 0.12486686988137648 0.0 0.0 47 0.13384422653951464 0.0 0.0 48 0.1418014290319553 0.0 0.0 49 0.14853444652555894 0.0 0.0 50 0.1562876181848601 0.0 0.0 51 0.1664891598418353 0.0 0.0 52 0.175670547333113 0.0 0.0 53 0.18648418148950668 0.0 0.0 54 0.19852200064473743 0.0 0.0 55 0.2119880356319447 0.0 0.0 56 0.22382182395403594 0.0 0.0 57 0.2368797972749642 0.0 0.0 58 0.24993777059589245 0.0 0.0 59 0.26870860724472684 0.0 0.0 60 0.28441898139646865 0.0 0.0 61 0.30094547888076845 0.0 0.0 62 0.32400096302553244 0.0 0.0 63 0.34522016967204083 0.0 0.0 64 0.3629708521551777 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGGGTC 15 0.002224141 70.0 35 ACGTATC 35 3.7071004E-9 70.0 48 CGCCGTA 13700 0.0 69.56569 46 GCCGTAT 13745 0.0 69.43979 47 CGTATCA 13740 0.0 69.43959 49 GTATCAT 13790 0.0 69.39086 50 CCGTATC 13750 0.0 69.33818 48 TGTAGGG 14240 0.0 69.3118 17 GTAGGGA 14295 0.0 69.26548 18 GTGTAGG 14275 0.0 69.215416 16 TAGATCT 14145 0.0 69.2082 32 TCGTGTA 14215 0.0 69.16286 14 TCGCCGT 13780 0.0 69.16183 45 TCTCGGT 14130 0.0 69.15782 36 GAGTGTA 14310 0.0 69.14395 27 GTGTAGA 14285 0.0 69.142456 29 AGCGTCG 14290 0.0 69.09377 10 GTAGATC 14200 0.0 69.08803 31 AGATCTC 14130 0.0 69.08352 33 CTCGGTG 14120 0.0 69.08286 37 >>END_MODULE