FastQCFastQC Report
Mon 15 Sep 2014
lane1_Undetermined_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane1_Undetermined_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490122
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG13089526.706615903795385Illumina Single End PCR Primer 1 (100% over 50bp)
CATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG15260.3113510513708832Illumina Single End PCR Primer 1 (98% over 50bp)
GCTCTTCCTCTCTTCTTTTCTTTCCCTCTTTTCTCTCTCTTTTTTCTCCT8570.17485442400055495No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5490.11201292739358772No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTC150.00222414170.035
ACGTATC353.7071004E-970.048
CGCCGTA137000.069.5656946
GCCGTAT137450.069.4397947
CGTATCA137400.069.4395949
GTATCAT137900.069.3908650
CCGTATC137500.069.3381848
TGTAGGG142400.069.311817
GTAGGGA142950.069.2654818
GTGTAGG142750.069.21541616
TAGATCT141450.069.208232
TCGTGTA142150.069.1628614
TCGCCGT137800.069.1618345
TCTCGGT141300.069.1578236
GAGTGTA143100.069.1439527
GTGTAGA142850.069.14245629
AGCGTCG142900.069.0937710
GTAGATC142000.069.0880331
AGATCTC141300.069.0835233
CTCGGTG141200.069.0828637
Produced by FastQC (version 0.11.2)