##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename lane1_Undetermined_L001_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 490122 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.421756215799334 34.0 34.0 34.0 34.0 34.0 2 33.51883612651544 34.0 34.0 34.0 34.0 34.0 3 33.48683797095417 34.0 34.0 34.0 34.0 34.0 4 33.548314501287436 34.0 34.0 34.0 34.0 34.0 5 33.597326788024205 34.0 34.0 34.0 34.0 34.0 6 37.28114428652458 38.0 38.0 38.0 37.0 38.0 7 37.224615503894945 38.0 38.0 38.0 37.0 38.0 8 37.18969766711145 38.0 38.0 38.0 37.0 38.0 9 37.25158429941933 38.0 38.0 38.0 37.0 38.0 10-11 37.25768278102187 38.0 38.0 38.0 37.0 38.0 12-13 37.22672212224711 38.0 38.0 38.0 37.0 38.0 14-15 37.210975022545405 38.0 38.0 38.0 37.0 38.0 16-17 37.249316496708985 38.0 38.0 38.0 37.0 38.0 18-19 37.217531553368346 38.0 38.0 38.0 37.0 38.0 20-21 37.24523077927536 38.0 38.0 38.0 37.0 38.0 22-23 36.98636359926712 38.0 38.0 38.0 36.5 38.0 24-25 37.22770657101701 38.0 38.0 38.0 37.0 38.0 26-27 37.19836591705739 38.0 38.0 38.0 37.0 38.0 28-29 37.19168798788873 38.0 38.0 38.0 37.0 38.0 30-31 37.11883979907043 38.0 38.0 38.0 37.0 38.0 32-33 37.0135048008455 38.0 38.0 38.0 37.0 38.0 34-35 34.1059338287202 38.0 37.0 38.0 21.5 38.0 36-37 33.47454409310335 38.0 36.0 38.0 11.0 38.0 38-39 33.16803571355703 38.0 34.5 38.0 11.0 38.0 40-41 35.5845952640363 38.0 36.0 38.0 30.0 38.0 42-43 36.27859900188116 38.0 37.0 38.0 34.0 38.0 44-45 36.72043491212392 38.0 38.0 38.0 36.0 38.0 46-47 36.694608689265124 38.0 38.0 38.0 36.0 38.0 48-49 36.721591766947824 38.0 38.0 38.0 36.0 38.0 50-51 36.68045405021607 38.0 38.0 38.0 36.0 38.0 52-53 36.66625350422956 38.0 38.0 38.0 35.5 38.0 54-55 36.77315443909883 38.0 38.0 38.0 35.5 38.0 56-57 36.74404433998066 38.0 38.0 38.0 35.5 38.0 58-59 36.78812724178878 38.0 38.0 38.0 36.0 38.0 60-61 36.539501389449974 38.0 38.0 38.0 35.0 38.0 62-63 36.56021051901364 38.0 38.0 38.0 35.5 38.0 64-65 36.5934787665112 38.0 38.0 38.0 35.5 38.0 66-67 36.70909487841803 38.0 38.0 38.0 36.0 38.0 68-69 36.6861689538523 38.0 38.0 38.0 36.0 38.0 70-71 36.67205002019905 38.0 38.0 38.0 35.0 38.0 72-73 36.33107777247298 38.0 38.0 38.0 34.0 38.0 74-75 32.460380272666804 38.0 27.0 38.0 16.0 38.0 76 31.199791072426866 38.0 23.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18151633841672066 1101 2 0.15798147294581355 1101 3 0.20802146566107638 1101 4 0.2114168224067967 1101 5 0.1288630099736494 1101 6 0.29126774205157346 1101 7 0.1071308814256966 1101 8 0.16179452782677117 1101 9 0.2164665122096352 1101 10-11 0.18962450480376702 1101 12-13 0.17886197410351912 1101 14-15 0.1814572321261636 1101 16-17 0.2223803071843662 1101 18-19 0.1129601169365344 1101 20-21 0.10399812116867935 1101 22-23 0.4314062908603802 1101 24-25 0.22775400812223268 1101 26-27 0.08847859511642753 1101 28-29 0.16697442549067176 1101 30-31 0.1552645835153541 1101 32-33 0.21436104473345097 1101 34-35 -0.25721160898477535 1101 36-37 -0.4311136133259055 1101 38-39 -0.5108047108032707 1101 40-41 -0.09250564691974006 1101 42-43 0.15998460819873372 1101 44-45 0.25839118787935433 1101 46-47 0.3632073761164136 1101 48-49 0.196402514513764 1101 50-51 0.3965866375839653 1101 52-53 -0.45608755387504374 1101 54-55 -0.4231995404174924 1101 56-57 -0.31028705031055637 1101 58-59 0.06048944723684002 1101 60-61 0.37416962206828686 1101 62-63 0.46599722227666973 1101 64-65 0.45034194994782695 1101 66-67 0.38671317635673574 1101 68-69 0.46193395406698556 1101 70-71 0.06649395876389264 1101 72-73 0.18500709746720645 1101 74-75 -0.009147750958511836 1101 76 0.1797670772464457 1102 1 -0.16557433609504812 1102 2 -0.1607728876265071 1102 3 -0.2153787710945707 1102 4 -0.16458694053284262 1102 5 -0.2506219558007672 1102 6 -0.30504755166207076 1102 7 -0.2554269018255937 1102 8 -0.32801276661720635 1102 9 -0.08439816585467952 1102 10-11 -0.043557742633701935 1102 12-13 -0.14743005516325525 1102 14-15 -0.1447389703519093 1102 16-17 -0.09631722235869233 1102 18-19 -0.037830136662861946 1102 20-21 -0.149458293663983 1102 22-23 0.198604025357902 1102 24-25 -0.20382989684661368 1102 26-27 -0.2816458769293533 1102 28-29 -0.24278182985234764 1102 30-31 -0.246465498115775 1102 32-33 0.03764979593808704 1102 34-35 -0.05443607824946639 1102 36-37 0.15058129352561878 1102 38-39 0.04235950789703935 1102 40-41 0.1034955042108976 1102 42-43 -0.03888197551881234 1102 44-45 -0.10455080940484862 1102 46-47 -0.17123525054120847 1102 48-49 -0.14226144844691646 1102 50-51 0.09476550538894202 1102 52-53 -0.12420945764197455 1102 54-55 -0.023720330296242764 1102 56-57 -0.07421035018612798 1102 58-59 -0.12299950638300317 1102 60-61 -0.15698557138090763 1102 62-63 -0.1154129248431488 1102 64-65 -0.08729046937327212 1102 66-67 0.0810447648565571 1102 68-69 0.026097999855863918 1102 70-71 -0.026814429375114912 1102 72-73 0.21197946670444878 1102 74-75 -0.1777073745678166 1102 76 -0.050057605074147915 1103 1 0.10152073546072415 1103 2 0.06279510287563284 1103 3 -0.08486189594706417 1103 4 0.016966617230295356 1103 5 0.01639444373864052 1103 6 -0.04325211320861655 1103 7 0.04289380822511646 1103 8 0.02250682629086498 1103 9 0.005938764935407903 1103 10-11 0.01794231815831182 1103 12-13 0.10419523717479962 1103 14-15 0.14707699434580945 1103 16-17 0.17086009721418804 1103 18-19 0.11197389849637318 1103 20-21 -0.018338862391132693 1103 22-23 0.31505291357927234 1103 24-25 0.02773581931905511 1103 26-27 0.04944887993835323 1103 28-29 0.04575851816403542 1103 30-31 -0.1051169167152679 1103 32-33 0.11838337673066945 1103 34-35 0.378521059805621 1103 36-37 0.17270422212719438 1103 38-39 0.3508235440575618 1103 40-41 0.19240382270383094 1103 42-43 0.2970743543600989 1103 44-45 0.21161593235783016 1103 46-47 0.22769087643542463 1103 48-49 0.16972673644662706 1103 50-51 0.2623011402363602 1103 52-53 0.04354073725006202 1103 54-55 0.10777100505394799 1103 56-57 -0.02381198370327553 1103 58-59 0.04636167684412129 1103 60-61 0.0442949944854476 1103 62-63 0.20197144517280208 1103 64-65 0.12872679555832178 1103 66-67 0.14817523881969663 1103 68-69 0.045816067957225926 1103 70-71 0.09962222892371386 1103 72-73 -0.10313414283918121 1103 74-75 0.6141890501466492 1103 76 0.07886794974236722 2116 1 0.036180049869862785 2116 2 -0.003675598950273695 2116 3 0.1083851842483341 2116 4 0.04354703075902222 2116 5 0.06995220687865356 2116 6 0.04662708948625749 2116 7 0.12655076962576572 2116 8 0.06752152365392305 2116 9 0.24096068581996377 2116 10-11 0.1761392270632669 2116 12-13 0.12250614898346868 2116 14-15 0.24242211417759307 2116 16-17 0.23821228322344012 2116 18-19 0.1918349351074653 2116 20-21 0.018404545251492266 2116 22-23 0.4099277893132509 2116 24-25 0.07846675387109059 2116 26-27 0.09076241653156814 2116 28-29 0.08696298673456937 2116 30-31 0.18615013186828122 2116 32-33 0.1357359203400037 2116 34-35 0.6269833794857789 2116 36-37 0.3953978804474758 2116 38-39 0.6886912463340451 2116 40-41 0.4384237338759718 2116 42-43 0.19915630199314904 2116 44-45 0.18372670255686785 2116 46-47 0.24264034754491348 2116 48-49 0.15837651008577325 2116 50-51 0.09955019438415746 2116 52-53 0.1401446093436718 2116 54-55 0.2123243268010384 2116 56-57 0.15784706608180699 2116 58-59 -0.011356763161309402 2116 60-61 -0.022216831349012978 2116 62-63 0.16130158871347078 2116 64-65 0.2229168671498556 2116 66-67 0.2959570455583105 2116 68-69 0.13973436349223078 2116 70-71 0.03777887206029362 2116 72-73 0.11628568680013984 2116 74-75 0.561128351110014 2116 76 0.45711942841867526 1117 1 -0.2276655976900912 1117 2 0.009034270425992474 1117 3 -0.3082500528538361 1117 4 -0.373088206343148 1117 5 -0.1472260837967454 1117 6 -0.014813395243905347 1117 7 -0.027468886793634795 1117 8 0.03071072470206815 1117 9 0.0761489797246 1117 10-11 -0.4523355886115823 1117 12-13 0.13306771518646343 1117 14-15 -0.4737116056777566 1117 16-17 -0.3494145751265947 1117 18-19 -0.1674438118512569 1117 20-21 -0.11729968652367262 1117 22-23 0.2470542396952098 1117 24-25 0.18385131616201988 1117 26-27 0.16295366111766896 1117 28-29 0.21963334113679878 1117 30-31 0.12893908049388614 1117 32-33 0.16811330189984375 1117 34-35 -0.11870830511422525 1117 36-37 -0.2150437603976485 1117 38-39 -0.20945293032229984 1117 40-41 0.2855663550770018 1117 42-43 0.33202755910178183 1117 44-45 0.2632570585860492 1117 46-47 0.2519866372540136 1117 48-49 0.15978837542427016 1117 50-51 0.4352610818318112 1117 52-53 -0.2584371161517467 1117 54-55 -0.08924789094298546 1117 56-57 -0.34484293487956563 1117 58-59 -0.0639889583746438 1117 60-61 0.292916319076582 1117 62-63 0.37275969865039116 1117 64-65 0.46276881541309933 1117 66-67 0.40502009942734674 1117 68-69 0.32796109513797944 1117 70-71 0.5028544632442404 1117 72-73 0.5468318956551457 1117 74-75 0.4283526152268209 1117 76 0.21056004133215822 2117 1 -0.4529488282802987 2117 2 -0.4871391066695452 2117 3 -0.5035329631749264 2117 4 -0.4670664351600138 2117 5 -0.5032520659257287 2117 6 -0.6097945408821346 2117 7 -0.46001427901062186 2117 8 -0.6130622353319026 2117 9 -0.3703986970787838 2117 10-11 -0.29048729179090316 2117 12-13 0.008666292242615725 2117 14-15 0.04745521776262507 2117 16-17 -0.0200584109542703 2117 18-19 0.09830216017145688 2117 20-21 -0.02976242558381159 2117 22-23 0.3443356886401574 2117 24-25 0.08143132709862044 2117 26-27 0.1590060476564048 2117 28-29 0.0777265418233668 2117 30-31 0.12312204431177776 2117 32-33 0.1597175062030729 2117 34-35 0.3093447195903849 2117 36-37 0.1462773378644755 2117 38-39 0.1522708767048826 2117 40-41 0.3927776294990011 2117 42-43 0.39966157690162873 2117 44-45 0.22288061188891106 2117 46-47 -0.0192243714632383 2117 48-49 0.22746746813743357 2117 50-51 0.450683819278737 2117 52-53 0.20267390929067375 2117 54-55 0.3001507266805987 2117 56-57 -0.020646069009458756 2117 58-59 -0.11303936043101004 2117 60-61 -0.4158309423258899 2117 62-63 -0.6447284780812339 2117 64-65 -0.7179685389979085 2117 66-67 -0.6413750343799052 2117 68-69 -0.49253340038477944 2117 70-71 -0.41782199258649655 2117 72-73 -0.3085087698640905 2117 74-75 -0.5445274107742861 2117 76 -0.34703146370733506 1116 1 0.20186624748013315 1116 2 0.21162606803387973 1116 3 0.21485665353257133 1116 4 0.1812441050393545 1116 5 0.16552646293608575 1116 6 0.23519836865287402 1116 7 0.23663783527797477 1116 8 0.2997600932522886 1116 9 0.22222158770209433 1116 10-11 0.2539660594198665 1116 12-13 0.30166305764857526 1116 14-15 0.31807043766888654 1116 16-17 0.14421331411662663 1116 18-19 0.3086190713048893 1116 20-21 0.24948896270346665 1116 22-23 0.6206035099113194 1116 24-25 0.3005476640451832 1116 26-27 0.1711823560973258 1116 28-29 0.2390850952846364 1116 30-31 0.41894990327892145 1116 32-33 0.4093932380007672 1116 34-35 0.4003054701072628 1116 36-37 0.46910767654726016 1116 38-39 0.33758154211821534 1116 40-41 0.5917896374575733 1116 42-43 0.6983513835868465 1116 44-45 0.4782745475792609 1116 46-47 0.522959252108862 1116 48-49 0.5315971921934306 1116 50-51 0.6123149677183903 1116 52-53 0.5638146264131692 1116 54-55 0.5277160101823739 1116 56-57 0.2942349807268485 1116 58-59 0.4124066319135622 1116 60-61 0.6518780556449215 1116 62-63 0.6127833109026994 1116 64-65 0.6233647080636189 1116 66-67 0.5079680716331865 1116 68-69 0.37062362091297985 1116 70-71 0.3610675333558575 1116 72-73 0.5964559335795343 1116 74-75 0.7004156528218175 1116 76 0.28125502822295445 2118 1 -0.2916363760344467 2118 2 -0.1284730048049667 2118 3 -0.4343925915705924 2118 4 -0.3260546212573985 2118 5 -0.1496674861537528 2118 6 -0.0964810661107478 2118 7 0.01730154621216684 2118 8 -0.03476692339657461 2118 9 0.010591603067531707 2118 10-11 -0.3383206178662661 2118 12-13 -0.1463467127212681 2118 14-15 -0.388919714299945 2118 16-17 -0.48717612750996864 2118 18-19 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62-63 0.2031525475350051 1119 64-65 0.3386234564577677 1119 66-67 0.1538712476706081 1119 68-69 0.22148724246847706 1119 70-71 0.1763797586719562 1119 72-73 0.31601066136970957 1119 74-75 -0.2660564684908806 1119 76 0.11300987034309884 2119 1 0.0437421013052699 2119 2 0.13014492537189426 2119 3 0.13882901399314562 2119 4 0.06305336050383659 2119 5 0.04189075352937266 2119 6 0.10355865846552348 2119 7 0.08307738445829926 2119 8 0.12825467756142217 2119 9 0.026489493634954897 2119 10-11 0.11568206498193234 2119 12-13 -0.01004593434986134 2119 14-15 0.11026190185702234 2119 16-17 0.13082716559983965 2119 18-19 -0.007395029007362552 2119 20-21 0.21118514732485494 2119 22-23 0.4577653341697072 2119 24-25 0.21890734398273537 2119 26-27 0.24763355389201536 2119 28-29 0.2694739516468516 2119 30-31 0.22990386990655765 2119 32-33 0.07181608880092227 2119 34-35 0.31245113267556945 2119 36-37 0.4901889241077555 2119 38-39 0.46614900650150304 2119 40-41 0.5266906547292436 2119 42-43 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-0.9637063734597575 2111 1 0.19350332163168105 2111 2 0.08834920949652769 2111 3 0.16228274173795398 2111 4 -0.023475293056996804 2111 5 0.08763212964213096 2111 6 0.022603076121306742 2111 7 0.14935613074909782 2111 8 0.12713728141898883 2111 9 0.12948003517798412 2111 10-11 0.08425935541219332 2111 12-13 0.04028557529405674 2111 14-15 -0.13989689821715956 2111 16-17 -0.09429106511232987 2111 18-19 -0.04385181684119033 2111 20-21 -0.03765109154754498 2111 22-23 0.2700856698522003 2111 24-25 0.07352529650498951 2111 26-27 0.13301119669616668 2111 28-29 0.030518091112725187 2111 30-31 -0.09433806258602573 2111 32-33 -0.04934836780144991 2111 34-35 -0.1479972167309569 2111 36-37 -0.19107614451242227 2111 38-39 -0.34969274980413445 2111 40-41 -0.525874488074912 2111 42-43 -0.1447804889997073 2111 44-45 -0.1437630158043106 2111 46-47 -0.5045620765082006 2111 48-49 -0.8683161150567926 2111 50-51 -0.7449503711651317 2111 52-53 -0.45653355761405834 2111 54-55 0.03858474450296967 2111 56-57 -0.22609583000383537 2111 58-59 0.05954513415008478 2111 60-61 -0.9507677236864112 2111 62-63 -0.531337160168448 2111 64-65 -1.081003170332579 2111 66-67 -0.5446958917485389 2111 68-69 -0.198096743138656 2111 70-71 -0.02262664334678277 2111 72-73 -0.39126404131026504 2111 74-75 -0.016362550408686616 2111 76 -0.7740470702396252 2110 1 0.1707256565516957 2110 2 0.13308485979160878 2110 3 0.22896340389775816 2110 4 0.09099329492354968 2110 5 0.13306598206673215 2110 6 0.1306887757151216 2110 7 0.0857477938382658 2110 8 0.19950616802246657 2110 9 0.09872965659203459 2110 10-11 0.040070354926285745 2110 12-13 0.018902545379866353 2110 14-15 -0.09837097274552065 2110 16-17 -0.025173182174405895 2110 18-19 -0.08721170229830477 2110 20-21 0.01128202729306338 2110 22-23 0.32373872943998094 2110 24-25 -0.022179698942544235 2110 26-27 0.04775092769964573 2110 28-29 -0.042196358369047005 2110 30-31 -0.13153914479932638 2110 32-33 0.1110853902715121 2110 34-35 0.1070370329140502 2110 36-37 -0.18818013888906648 2110 38-39 0.01765002498321877 2110 40-41 -0.19200531605772397 2110 42-43 0.13249774926648428 2110 44-45 0.1304605881452474 2110 46-47 0.2969403554052903 2110 48-49 0.22994463041550262 2110 50-51 0.3586447801041288 2110 52-53 0.08789780864486119 2110 54-55 0.2235139571857161 2110 56-57 0.1724749785425388 2110 58-59 0.14996230992247206 2110 60-61 0.3374240507376882 2110 62-63 0.3867886152840896 2110 64-65 0.2576838322573778 2110 66-67 0.3806253755826745 2110 68-69 0.13870617515753736 2110 70-71 0.24018807833946454 2110 72-73 0.06633551146296668 2110 74-75 0.30804315220439094 2110 76 0.07794233407130235 2109 1 0.10270385323577358 2109 2 -0.006024838141620137 2109 3 -0.01107747731959563 2109 4 0.07311911822584705 2109 5 0.04548932978420339 2109 6 0.02978030421841993 2109 7 0.04884113928455491 2109 8 0.12090067176991681 2109 9 0.04501627934924102 2109 10-11 7.22183225171591E-4 2109 12-13 0.06214578862311271 2109 14-15 -0.002447738586994319 2109 16-17 0.09276912988555353 2109 18-19 0.01747828180344868 2109 20-21 -0.06178179282957785 2109 22-23 0.17547618117743013 2109 24-25 -0.007950764125375542 2109 26-27 -0.1500573848145521 2109 28-29 -0.046295835997938184 2109 30-31 -0.5646756426417028 2109 32-33 -0.035099029442314134 2109 34-35 -0.5438260150078236 2109 36-37 -0.5924953784984197 2109 38-39 -0.5994292391548655 2109 40-41 -0.40429239305068165 2109 42-43 0.03774417215996806 2109 44-45 0.09803951160652247 2109 46-47 0.14624764588905492 2109 48-49 0.08961985185543142 2109 50-51 0.34213331065148367 2109 52-53 0.1505142065553713 2109 54-55 0.13455188587070666 2109 56-57 0.1768583535141488 2109 58-59 0.2030284012238539 2109 60-61 0.19767842275676628 2109 62-63 0.3087282019295543 2109 64-65 0.31446330080154894 2109 66-67 0.2987607378356856 2109 68-69 0.012122027985149941 2109 70-71 0.08920011558679874 2109 72-73 -0.48860430230037366 2109 74-75 -0.12219759274223918 2109 76 -0.528825778579165 2108 1 0.09691532661391022 2108 2 -0.025690336973781314 2108 3 0.0016858812770976783 2108 4 0.07187859198531754 2108 5 0.132197466492336 2108 6 0.028008889947003013 2108 7 -0.11956536245528326 2108 8 0.06511810348734315 2108 9 -0.06554368537739208 2108 10-11 9.202980066191913E-4 2108 12-13 0.14526849466248137 2108 14-15 -0.00837717993310605 2108 16-17 0.07979514399490029 2108 18-19 0.08868907593090825 2108 20-21 0.07235412880633874 2108 22-23 0.171909817194404 2108 24-25 0.1404351217605111 2108 26-27 -0.011196500120327357 2108 28-29 -0.03673746810623868 2108 30-31 0.0564374347213672 2108 32-33 0.006601003924380677 2108 34-35 -0.3849543457194926 2108 36-37 -0.5368799416329821 2108 38-39 -0.43594879442441936 2108 40-41 -0.6906945690361894 2108 42-43 -0.21248939474026685 2108 44-45 -0.29801874903507297 2108 46-47 -0.2760719951805797 2108 48-49 -0.186458905473323 2108 50-51 -0.036730374462202064 2108 52-53 -0.06809458080341102 2108 54-55 -0.2033362999341506 2108 56-57 -0.31999236625323846 2108 58-59 -0.09903914251035673 2108 60-61 -0.3453291681674884 2108 62-63 -0.13097608569139396 2108 64-65 -0.03500223616399012 2108 66-67 -0.0716093346177189 2108 68-69 -0.5837080282615617 2108 70-71 -0.3122418025217826 2108 72-73 -1.658245074024478 2108 74-75 -0.5681173219953024 2108 76 -1.0255582253116096 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 5.0 12 22.0 13 45.0 14 47.0 15 84.0 16 130.0 17 343.0 18 606.0 19 831.0 20 974.0 21 850.0 22 662.0 23 817.0 24 964.0 25 1381.0 26 1913.0 27 2916.0 28 4149.0 29 4611.0 30 5224.0 31 6631.0 32 9086.0 33 12597.0 34 26239.0 35 69811.0 36 50879.0 37 288304.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.87709848280815 18.990810338776942 16.10720727338018 19.024883905034727 2 16.819893822354434 49.763120202725034 18.856121537086683 14.560864437833846 3 15.831772497459816 19.480455886493566 48.230032522514804 16.457739093531814 4 16.44366096604519 19.720804207931902 17.717221426501972 46.118313399520936 5 47.41737771412016 19.898107001930132 18.210772011866432 14.473743272083277 6 46.364578615120315 20.23598206160915 17.66947821154733 15.729961111723204 7 16.023357449777812 49.00494162677864 19.637151566344706 15.334549357098846 8 15.574693647704041 49.19183386993443 19.033016269418635 16.2004562129429 9 45.97243135382619 18.186492342722833 19.8860691827749 15.955007120676076 10-11 31.161833176229592 35.05974022794325 17.950530684197 15.827895911630167 12-13 15.774133787097908 34.50855093221688 18.560276829034404 31.157038451650816 14-15 15.818714524138889 34.56425134966396 18.362774982555365 31.25425914364179 16-17 31.243241478652255 19.424551438213342 18.44346917706204 30.888737906072368 18-19 16.02713202019089 19.267651727529064 33.595104892251314 31.110111360028725 20-21 31.312509946503113 19.086778393950894 33.6386654751266 15.96204618441939 22-23 15.852277596190337 49.284871929846034 18.22076952269027 16.642080951273357 24-25 16.07752763597635 19.283260086264235 33.48411211902343 31.155100158735987 26-27 16.046616964755714 19.325392453307543 18.242906868085907 46.38508371385084 28-29 31.189989431202843 34.2919722028393 18.276469940137353 16.24156842582051 30-31 16.04845324225397 19.42506151529619 33.18071827014498 31.345766972304855 32-33 16.12185166709003 34.15007125798656 18.457705809081645 31.270371265841767 34-35 21.9512916695146 25.745554928726854 28.23636588070509 24.066787521053453 36-37 24.43718094678468 30.413958157356742 23.72541938537752 21.423441510481066 38-39 22.992045817397592 27.113183621545357 25.970911175743876 23.923859385313172 40-41 16.16729508932579 33.266410400803075 32.69175052436566 17.874543985505472 42-43 16.28260714322149 19.225283861950768 33.19261805902696 31.299490935800783 44-45 30.6318529974659 19.4014045711814 19.958825905809668 30.00791652554304 46-47 16.882756509984954 33.74082481063021 32.99069091280063 16.385727766584203 48-49 30.89464153638195 19.58223877171058 32.0885510979622 17.43456859394527 50-51 17.971810524941237 19.30048641943066 18.304489586053798 44.4232134695743 52-53 30.226762181275085 19.15073339631149 33.08067257409965 17.54183184831377 54-55 16.119386845289224 19.197402274132305 34.452002668728746 30.231208211849726 56-57 16.282678288334058 19.128109703969855 33.84116918544272 30.748042822253367 58-59 30.784533159770216 19.203683999514404 32.43260062086899 17.579182219846384 60-61 17.00754113345521 19.563079459388874 33.57508079785845 29.85429860929747 62-63 31.058105086184383 20.614267759206058 32.42207903499608 15.905548119613478 64-65 16.698938201880388 49.180391420736484 18.008740859232176 16.11192951815095 66-67 16.10562429799567 49.66696557641123 18.057719165623542 16.169690959969557 68-69 16.053240457361113 49.51654302246815 18.11683356593133 16.313382954239405 70-71 16.068595795906084 49.622386468550914 18.139119700888052 16.169898034654956 72-73 15.970611218908063 49.345363064888325 18.307492670147454 16.376533046056164 74-75 16.62286733290622 44.48414042447267 20.423003627727425 18.46998861489368 76 16.909161764735885 43.31939777153432 20.8870199321381 18.884420531591694 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 854.0 1 486.0 2 86.5 3 53.0 4 51.0 5 39.5 6 36.0 7 40.0 8 36.0 9 32.5 10 33.0 11 35.5 12 34.0 13 38.0 14 49.0 15 52.5 16 60.5 17 72.0 18 84.5 19 104.0 20 120.5 21 130.0 22 170.5 23 236.0 24 327.5 25 394.0 26 467.0 27 628.5 28 839.0 29 961.0 30 1186.0 31 1845.5 32 2454.5 33 2629.0 34 3222.0 35 4906.0 36 7683.5 37 9370.0 38 10927.0 39 15875.5 40 23145.5 41 39222.0 42 51420.0 43 72605.5 44 76749.0 45 50571.5 46 41436.0 47 37191.0 48 28711.5 49 22730.0 50 20983.0 51 18685.5 52 13862.5 53 10469.5 54 9602.0 55 8648.5 56 6878.5 57 5152.5 58 4243.0 59 3937.5 60 3515.5 61 3038.5 62 2678.0 63 2489.5 64 2194.0 65 1869.5 66 1393.0 67 1134.0 68 839.5 69 601.5 70 748.0 71 838.0 72 779.5 73 584.0 74 314.0 75 181.0 76 151.5 77 79.5 78 28.5 79 20.0 80 18.0 81 9.0 82 2.0 83 2.0 84 2.0 85 3.5 86 6.5 87 8.0 88 10.5 89 14.5 90 20.0 91 32.5 92 41.0 93 26.5 94 14.5 95 16.5 96 16.0 97 18.5 98 28.5 99 92.0 100 148.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0012241849988370243 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 3.0604624970925607E-4 34-35 1.0201541656975203E-4 36-37 0.0 38-39 5.100770828487601E-4 40-41 0.0012241849988370243 42-43 0.0014282158319765283 44-45 0.0016322466651160325 46-47 0.0019382929148252883 48-49 0.0019382929148252883 50-51 0.0020403083313950405 52-53 0.0010201541656975203 54-55 2.0403083313950406E-4 56-57 6.120924994185121E-4 58-59 5.100770828487601E-4 60-61 0.0020403083313950405 62-63 0.0020403083313950405 64-65 0.0020403083313950405 66-67 0.0015302312485462803 68-69 0.0012241849988370243 70-71 9.181387491277683E-4 72-73 6.120924994185121E-4 74-75 0.0016322466651160325 76 0.0018362774982555365 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 490122.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.35827695073692 #Duplication Level Percentage of deduplicated Percentage of total 1 84.55960612384801 27.362031538007038 2 3.3413444170870186 2.1624029607180075 3 1.5702843384239773 1.524350865423833 4 0.9462227029332243 1.2247254511435257 5 0.7658294163323398 1.239046017535153 6 0.6025818015925454 1.1699105292843353 7 0.5722501958782035 1.296192122633825 8 0.48815135773153523 1.2636589461884216 9 0.5412051190348658 1.5761218615998056 >10 6.40936024409851 36.03402399427793 >50 0.08053627677736178 1.8491089144688857 >100 0.10046503130219174 7.3052500506274685 >500 0.013297792015714375 2.9677957949333367 >1k 0.007598728238073532 4.253139619072862 >5k 6.33227353172051E-4 1.1882235704092858 >10k+ 6.33227353172051E-4 7.5840177636763055 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC 37013 7.551793226992463 TruSeq Adapter, Index 3 (100% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC 5799 1.183174801375984 TruSeq Adapter, Index 6 (100% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAATATCTCGTATGC 2837 0.578835473616773 TruSeq Adapter, Index 19 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC 2707 0.5523114653086374 TruSeq Adapter, Index 7 (100% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAATATCTCGTATGC 2198 0.44845977124062986 TruSeq Adapter, Index 6 (98% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAACATCTCGTATGC 1927 0.39316741545982425 TruSeq Adapter, Index 19 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAATATCTCGTATGC 1697 0.34624032383773834 TruSeq Adapter, Index 6 (98% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCATATCTCGTATGCC 1549 0.31604376053309174 TruSeq Adapter, Index 6 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGAATATCTCGTATGC 1528 0.3117591130371622 TruSeq Adapter, Index 6 (98% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAACATCTCGTATGC 1515 0.3091067122063486 TruSeq Adapter, Index 7 (98% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC 1372 0.2799303030673995 TruSeq Adapter, Index 8 (100% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC 1225 0.24993777059589248 TruSeq Adapter, Index 9 (100% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAACATCTCGTATGC 1141 0.2327991806121741 TruSeq Adapter, Index 6 (98% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAATATCTCGTATGC 1061 0.21647671396101378 TruSeq Adapter, Index 7 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAACATCTCGTATGC 981 0.20015424730985346 TruSeq Adapter, Index 6 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTATATCTCGTATGC 959 0.19566556898078438 TruSeq Adapter, Index 8 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAAGATCTCGTATGC 929 0.18954464398659926 TruSeq Adapter, Index 19 (97% over 38bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 914 0.18648418148950668 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATATCTCGTATGCC 871 0.177710855664508 TruSeq Adapter, Index 6 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCAGATCTCGTATGC 817 0.1666931906749748 TruSeq Adapter, Index 9 (98% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCCATATCTCGTATGC 745 0.1520029706889305 TruSeq Adapter, Index 6 (98% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC 740 0.150982816523233 TruSeq Adapter, Index 10 (100% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTTCATCTCGTATGC 709 0.14465786069590836 TruSeq Adapter, Index 8 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTATCTCGTATGC 645 0.1315998873749801 TruSeq Adapter, Index 7 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCCAATATCTCGTATGC 633 0.12915151737730607 TruSeq Adapter, Index 6 (98% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATTATCTCGTATGC 625 0.12751927071219002 TruSeq Adapter, Index 7 (98% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAAGATCTCGTATGC 588 0.11997012988602838 TruSeq Adapter, Index 9 (98% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGATCATCTCGTATGC 585 0.11935803738660987 TruSeq Adapter, Index 7 (98% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGAACATCTCGTATGC 578 0.11792982155463334 TruSeq Adapter, Index 6 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACCATATCTCGTATGC 545 0.1111968040610297 TruSeq Adapter, Index 9 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTCATCTCGTATGC 535 0.10915649572963465 TruSeq Adapter, Index 9 (97% over 36bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATCATCTCGTATGC 535 0.10915649572963465 TruSeq Adapter, Index 7 (98% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAAGATCTCGTATGC 531 0.10834037239707665 TruSeq Adapter, Index 6 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATATCATCTCGTATGC 515 0.10507587906684458 TruSeq Adapter, Index 7 (98% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGATCTCGTATGC 504 0.10283153990231003 TruSeq Adapter, Index 6 (98% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGCATCTCGTATGC 498 0.10160735490347302 TruSeq Adapter, Index 8 (98% over 50bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.006528986660464129 0.0 0.0 2 0.006733017493603634 0.0 0.0 3 0.008161233325580162 0.0 0.0 4 0.009181387491277683 0.0 0.0 5 0.01060960332325421 0.0 0.0 6 0.01162975748895173 0.0 0.0 7 0.012037819155230738 0.0 0.0 8 0.012853942487788754 0.0 0.0 9 0.014078127486625779 0.0 0.0 10 0.017342620816857845 0.0 0.0 11 0.018566805815694868 0.0 0.0 12 0.018974867481973877 0.0 0.0 13 0.022443391645345444 0.0 0.0 14 0.024891761643019494 0.0 0.0 15 0.026319977474996023 0.0 0.0 16 0.033052994968599654 0.0 0.0 17 0.03386911830115767 0.0 0.0 18 0.03427717996743668 0.0 0.0 19 0.037949734963947754 0.0 0.0 20 0.03856182746336626 0.0 0.0 21 0.03917391996278478 0.0 0.0 22 0.03958198162906378 0.0 0.0 23 0.0403981049616218 0.0 0.0 24 0.041010197461040315 0.0 0.0 25 0.04284647495929585 0.0 0.0 26 0.04427469079127238 0.0 0.0 27 0.04713112245522543 0.0 0.0 28 0.052639954949992046 0.0 0.0 29 0.057332664112200636 0.0 0.0 30 0.06937048326743138 0.0 0.0 31 0.07202288409824492 0.0 0.0 32 0.07426722326277947 0.0 0.0 33 0.07936799409126707 0.0 0.0 34 0.08161233325580161 0.0 0.0 35 0.08671310408428921 0.0 0.0 36 0.0905896899139398 0.0 0.0 37 0.09222193657905582 0.0 0.0 38 0.09813883074010145 0.0 0.0 39 0.10181138573661251 0.0 0.0 40 0.1058920023994026 0.0 0.0 41 0.1109927732278902 0.0 0.0 42 0.11568548239009879 0.0 0.0 43 0.1207862532185864 0.0 0.0 44 0.126703147379632 0.0 0.0 45 0.13506841153835167 0.0 0.0 46 0.15220700152207 0.0 0.0 47 0.16404078984416126 0.0 0.0 48 0.18485193482439066 0.0 0.0 49 0.19444138398194735 0.0 0.0 50 0.20301067897380654 0.0 0.0 51 0.2162726831278743 0.0 0.0 52 0.22606616311857047 0.0 0.0 53 0.2368797972749642 0.0 0.0 54 0.24871358559705542 0.0 0.0 55 0.2623836514174022 0.0 0.0 56 0.27482953223891193 0.0 0.0 57 0.2889076597255377 0.0 0.0 58 0.3017616022133265 0.0 0.0 59 0.3221646855272769 0.0 0.0 60 0.3393032755109952 0.0 0.0 61 0.35623783466157405 0.0 0.0 62 0.38174168880401205 0.0 0.0 63 0.40173671045168347 0.0 0.0 64 0.41989545460109934 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCTTAG 40 1.3460522E-10 70.00317 33 ACGCCTA 90 0.0 69.99602 32 GCACACG 14795 0.0 69.12077 11 CACGTCT 14780 0.0 69.04885 14 AGCACAC 14835 0.0 68.98159 10 ACGTCTG 14785 0.0 68.95448 15 CACGCTA 330 0.0 68.93548 31 ACACGTC 14835 0.0 68.84004 13 CAGTCAC 14220 0.0 68.81466 27 CTGAACT 14385 0.0 68.80387 19 CACACGT 14850 0.0 68.79407 12 CGTCTGA 14455 0.0 68.78544 16 TCGGAAG 14990 0.0 68.618515 3 AGAGCAC 14930 0.0 68.56609 8 CGGAAGA 15010 0.0 68.55041 4 CTCCAGT 14665 0.0 68.54025 24 ATCGGAA 15020 0.0 68.50476 2 CCAGTCA 14465 0.0 68.44755 26 TCCAGTC 14645 0.0 68.39488 25 ACTCCAG 14725 0.0 68.379814 23 >>END_MODULE