FastQCFastQC Report
Mon 15 Sep 2014
TGA_6_CAGATC_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameTGA_6_CAGATC_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2222550
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG109680749.349036017187466Illumina Single End PCR Primer 1 (100% over 50bp)
CATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG57220.25745202582619064Illumina Single End PCR Primer 1 (98% over 50bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC24250.10910890643630064No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG22830.10271984882229872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC1145650.068.9735143
GCCGTAT1142450.068.9645147
CGCCGTA1142750.068.96171646
CGTATCA1143750.068.9442749
CCGTATC1141500.068.94218448
TGGTCGC1149450.068.89468442
GTGGTCG1154500.068.878341
GAGCGTC1162300.068.870779
TCGTGTA1159150.068.86468514
GTATCAT1146950.068.8617650
TGTAGGG1162800.068.85319517
TCGGAAG1168300.068.852613
GTCGTGT1161300.068.8336313
AGCGTCG1163350.068.8146310
CATTAAA1150050.068.8070154
TATCATT1145500.068.7992251
TCATTAA1148600.068.79636453
GTCGCCG1149550.068.7943144
CGTCGTG1161400.068.7794912
CGTGTAG1162600.068.7777415
Produced by FastQC (version 0.11.2)