##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	TGA_6_CAGATC_L001_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2222550
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.76635441272413	34.0	34.0	34.0	34.0	34.0
2	33.78084182583069	34.0	34.0	34.0	34.0	34.0
3	33.710126656318195	34.0	34.0	34.0	34.0	34.0
4	33.76932532451463	34.0	34.0	34.0	34.0	34.0
5	33.83359429484151	34.0	34.0	34.0	34.0	34.0
6	37.50682909270883	38.0	38.0	38.0	37.0	38.0
7	37.41519155924501	38.0	38.0	38.0	37.0	38.0
8	37.415978493172254	38.0	38.0	38.0	37.0	38.0
9	37.45441047445502	38.0	38.0	38.0	37.0	38.0
10-11	37.50574835211806	38.0	38.0	38.0	37.0	38.0
12-13	37.44302962812985	38.0	38.0	38.0	37.0	38.0
14-15	37.43880092686329	38.0	38.0	38.0	37.0	38.0
16-17	37.46242851679377	38.0	38.0	38.0	37.0	38.0
18-19	37.45940856223707	38.0	38.0	38.0	37.0	38.0
20-21	37.503892600841375	38.0	38.0	38.0	37.0	38.0
22-23	37.22724370655328	38.0	38.0	38.0	37.0	38.0
24-25	37.4405678162471	38.0	38.0	38.0	37.0	38.0
26-27	37.40162043598569	38.0	38.0	38.0	37.0	38.0
28-29	37.48434028480799	38.0	38.0	38.0	37.0	38.0
30-31	37.34760590312929	38.0	38.0	38.0	37.0	38.0
32-33	37.303446266675664	38.0	38.0	38.0	37.0	38.0
34-35	36.657492069919684	38.0	38.0	38.0	35.0	38.0
36-37	36.66819464129041	38.0	38.0	38.0	35.0	38.0
38-39	36.068745809993025	38.0	37.0	38.0	34.0	38.0
40-41	35.956297946052956	37.5	36.5	38.0	31.0	38.0
42-43	36.44737306247329	38.0	37.0	38.0	34.5	38.0
44-45	37.148712964837685	38.0	38.0	38.0	36.5	38.0
46-47	37.24162133585296	38.0	38.0	38.0	36.5	38.0
48-49	37.294558952554496	38.0	38.0	38.0	37.0	38.0
50-51	37.11416886009314	38.0	38.0	38.0	36.5	38.0
52-53	37.138908236035185	38.0	38.0	38.0	36.0	38.0
54-55	37.11205192234145	38.0	38.0	38.0	36.0	38.0
56-57	37.08615554205754	38.0	38.0	38.0	36.5	38.0
58-59	37.194921824030956	38.0	38.0	38.0	36.5	38.0
60-61	37.020926638320844	38.0	38.0	38.0	36.0	38.0
62-63	37.054322512429415	38.0	38.0	38.0	36.0	38.0
64-65	36.99372297586106	38.0	38.0	38.0	36.0	38.0
66-67	37.11332028525793	38.0	38.0	38.0	36.5	38.0
68-69	37.02589862995208	38.0	38.0	38.0	36.0	38.0
70-71	36.908773030977926	38.0	38.0	38.0	36.0	38.0
72-73	36.54836201660255	38.0	38.0	38.0	34.0	38.0
74-75	29.35581179276057	33.0	23.5	38.0	11.0	38.0
76	27.542541225169288	27.0	21.0	38.0	11.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.001502159358828692
1101	2	0.039997516523122556
1101	3	-0.08093104056631262
1101	4	0.07755423931325822
1101	5	-0.002117532199676475
1101	6	0.028855325336515136
1101	7	-0.054934608483684144
1101	8	-0.06999149020496986
1101	9	0.005736871952038314
1101	10-11	3.5118609845596893E-4
1101	12-13	-0.030969899501016585
1101	14-15	-0.036750856358118256
1101	16-17	0.039382643484920266
1101	18-19	-0.0017843829085393281
1101	20-21	-0.028728176470430355
1101	22-23	0.2671135950515975
1101	24-25	-0.007730504024230811
1101	26-27	0.010851007484454556
1101	28-29	-0.03692747827052045
1101	30-31	-0.0688849218482872
1101	32-33	0.01989939084455017
1101	34-35	-0.002930625067335768
1101	36-37	-0.020990729904333705
1101	38-39	-0.01086995550348746
1101	40-41	-0.0011392155260381287
1101	42-43	0.012920714722532978
1101	44-45	0.028186623662527666
1101	46-47	0.016963308802800725
1101	48-49	0.009824580468347222
1101	50-51	0.02006291632467594
1101	52-53	-0.10001371390939084
1101	54-55	-0.0014519121877185626
1101	56-57	0.033806154281229794
1101	58-59	-0.0670539854931107
1101	60-61	0.015342320168066692
1101	62-63	-0.07533998873763892
1101	64-65	-0.05355551678461978
1101	66-67	-0.032209021195818366
1101	68-69	0.10294069102117476
1101	70-71	-0.037049765919888955
1101	72-73	0.2620741966544813
1101	74-75	-0.05109537671323494
1101	76	0.30179780133570944
1102	1	0.043983354567345145
1102	2	0.05328614939908505
1102	3	0.015034249012785494
1102	4	0.05299105849855579
1102	5	0.034619910671388254
1102	6	0.009725177681517039
1102	7	-0.018231654749833126
1102	8	-0.15566570529617252
1102	9	0.003026924731656777
1102	10-11	0.06218015340090233
1102	12-13	0.10330664114343335
1102	14-15	-0.01449811638777021
1102	16-17	0.035550430688381596
1102	18-19	0.06432094927919252
1102	20-21	-0.15765639411677057
1102	22-23	0.24047708981252924
1102	24-25	-0.1066964349802646
1102	26-27	-0.09168709912241724
1102	28-29	-0.12283481918397143
1102	30-31	-0.09841350115515013
1102	32-33	0.1150863121718615
1102	34-35	0.13940497815256947
1102	36-37	0.07977821650294459
1102	38-39	0.17634108632201162
1102	40-41	0.14228495648976036
1102	42-43	0.2343122491232137
1102	44-45	0.15399622518477685
1102	46-47	0.05728198900695958
1102	48-49	0.012750524194295565
1102	50-51	0.16390823326457848
1102	52-53	0.05371851784607884
1102	54-55	0.20282233494351942
1102	56-57	0.2082859503314296
1102	58-59	0.11463470293426781
1102	60-61	0.07737551387369734
1102	62-63	0.02499700787798531
1102	64-65	0.07694223032846281
1102	66-67	0.057300691371658274
1102	68-69	0.11836511486241363
1102	70-71	0.09229747889651208
1102	72-73	0.13740476797553924
1102	74-75	-0.46384075525529056
1102	76	-7.007738624231763E-4
1103	1	-0.0030986840482043476
1103	2	0.013500494025493026
1103	3	-0.049018451735591384
1103	4	-0.015696880252846768
1103	5	0.05793092355496299
1103	6	0.023016113303889085
1103	7	0.061193776380150666
1103	8	-0.04126300200793054
1103	9	0.05749663712738595
1103	10-11	0.00583092396512086
1103	12-13	0.08615423732086924
1103	14-15	0.134118104007392
1103	16-17	0.1891738531660323
1103	18-19	0.15226392655772258
1103	20-21	0.029574884557149517
1103	22-23	0.3350950436859179
1103	24-25	-0.01208516725599651
1103	26-27	0.027894938407719394
1103	28-29	0.01889113640349649
1103	30-31	0.08351088544494445
1103	32-33	0.10326058389898662
1103	34-35	0.16164448498683726
1103	36-37	0.18358955305691183
1103	38-39	0.2421759207826497
1103	40-41	0.10589956974885695
1103	42-43	0.246013560028139
1103	44-45	0.15011602997816453
1103	46-47	0.07797534556525676
1103	48-49	-0.012360640270934198
1103	50-51	0.07588882838105349
1103	52-53	-0.07823321394613458
1103	54-55	0.08068843163768236
1103	56-57	-0.07459992477333088
1103	58-59	-0.049593791436123524
1103	60-61	-0.035627425282207525
1103	62-63	0.09644783132355883
1103	64-65	-0.21719720077286553
1103	66-67	-0.06888168969918951
1103	68-69	-0.2340217318324349
1103	70-71	-0.09553066160002999
1103	72-73	-0.33991250007550633
1103	74-75	0.49391619801417264
1103	76	-0.22751833909977393
2116	1	-0.050834585544201616
2116	2	-0.004159954823549583
2116	3	0.1325779192938512
2116	4	0.056882417529614315
2116	5	0.05145814403952187
2116	6	-0.052301171716997885
2116	7	0.05085436002381272
2116	8	-0.04009940565358505
2116	9	0.04675618410061588
2116	10-11	0.030249087494020444
2116	12-13	0.034625889917023756
2116	14-15	0.16355462961492861
2116	16-17	0.17853601367408345
2116	18-19	0.18771959050430098
2116	20-21	0.07753232349805472
2116	22-23	0.3832533271695411
2116	24-25	0.030545686062559696
2116	26-27	0.1406921184090777
2116	28-29	0.08086440701724484
2116	30-31	0.13658532877750673
2116	32-33	0.12077157297886743
2116	34-35	-0.011895624381736525
2116	36-37	-0.07025993661554963
2116	38-39	-0.13117319267328043
2116	40-41	0.03997127424320013
2116	42-43	0.09417728408563164
2116	44-45	0.02625723856959894
2116	46-47	-0.053829520282057786
2116	48-49	0.026148198278875157
2116	50-51	0.03319898823577461
2116	52-53	-0.09509497917578358
2116	54-55	0.10290919049618452
2116	56-57	0.08617514596122078
2116	58-59	-0.10152686307350223
2116	60-61	-0.15003513428604975
2116	62-63	0.00715709323965541
2116	64-65	-0.059110235894301866
2116	66-67	0.04069766600638758
2116	68-69	-0.10301218824892544
2116	70-71	-0.12029854794048589
2116	72-73	0.10347356333004143
2116	74-75	-0.054772215214377695
2116	76	-0.45283658572365226
1117	1	-0.0119454312361853
1117	2	0.08087353040988177
1117	3	0.07837887062308369
1117	4	0.0077944302521189
1117	5	-0.010607367767306641
1117	6	-0.05044954584575834
1117	7	-0.06588302929475276
1117	8	-0.03786446583725933
1117	9	0.014319675757818118
1117	10-11	-8.522139284323771E-4
1117	12-13	0.04547500134216875
1117	14-15	0.14284669377205006
1117	16-17	-0.018789338749535034
1117	18-19	0.22935574741571685
1117	20-21	0.056552497547578184
1117	22-23	0.46291507873575455
1117	24-25	0.07978643802640306
1117	26-27	0.15084296239731287
1117	28-29	0.11594274365509705
1117	30-31	0.11992889577413735
1117	32-33	0.08305824124412453
1117	34-35	0.2207796843065637
1117	36-37	0.2110710314441988
1117	38-39	0.39357679341831187
1117	40-41	0.5308627919266939
1117	42-43	0.5614097499995054
1117	44-45	0.22916391877814846
1117	46-47	0.1433837392200914
1117	48-49	0.13455334235599992
1117	50-51	0.3133217614459767
1117	52-53	0.26848034769452056
1117	54-55	0.36923424186048237
1117	56-57	0.3034579640040249
1117	58-59	0.34363212352448613
1117	60-61	0.2973028453183346
1117	62-63	0.39973506131187264
1117	64-65	0.3035408012741456
1117	66-67	0.4338661789937035
1117	68-69	0.34112876570995354
1117	70-71	0.46908119298222317
1117	72-73	0.5333518247799418
1117	74-75	0.23394454319841884
1117	76	0.036429769664263034
2117	1	0.02656543035376302
2117	2	0.021066783503620456
2117	3	-0.06527693797632139
2117	4	0.0017058204749602623
2117	5	-0.05567839014124587
2117	6	-0.08423750691677157
2117	7	0.02374849923015887
2117	8	-0.04558679654382303
2117	9	0.035843284724762725
2117	10-11	-0.02460333921080604
2117	12-13	0.10780481069586756
2117	14-15	0.09989602617999793
2117	16-17	0.017470972474704638
2117	18-19	0.15551692164041242
2117	20-21	0.021391249849727956
2117	22-23	0.34930793639725266
2117	24-25	0.07980098887464493
2117	26-27	0.13017753715380564
2117	28-29	0.0987060273789524
2117	30-31	0.216594206566306
2117	32-33	0.17687062243435747
2117	34-35	0.14477966935312025
2117	36-37	0.15203696046050652
2117	38-39	0.12747129775560495
2117	40-41	0.36342373690364127
2117	42-43	0.3796443352665406
2117	44-45	0.10328813959963412
2117	46-47	-0.12650497698530927
2117	48-49	0.0652921099135284
2117	50-51	0.24889398433678878
2117	52-53	0.1376264961554412
2117	54-55	0.2437729030472866
2117	56-57	-0.07066553790203045
2117	58-59	0.23212130981431045
2117	60-61	0.154339783624323
2117	62-63	0.27730507253127
2117	64-65	0.239188567798017
2117	66-67	0.15704514824439286
2117	68-69	0.11762552246678126
2117	70-71	0.30096697572511744
2117	72-73	0.3185580262594385
2117	74-75	0.06989050146307818
2117	76	-0.20049407174241907
1116	1	-2.2754741588215666E-4
1116	2	0.024816150164660655
1116	3	-0.010342552394263294
1116	4	0.05523708360460944
1116	5	0.05674869082400136
1116	6	0.034669550223327406
1116	7	0.015931151823487255
1116	8	0.12306734759517468
1116	9	0.037567571091244645
1116	10-11	0.1246453134263561
1116	12-13	0.12787016368466197
1116	14-15	0.08311320618606999
1116	16-17	-0.10148650825940564
1116	18-19	-0.016964244359357394
1116	20-21	-0.036123477206068344
1116	22-23	0.3350105984908467
1116	24-25	0.05530209840858191
1116	26-27	0.028570499968267882
1116	28-29	0.02699787513006413
1116	30-31	0.17547170287442526
1116	32-33	0.18736799818712058
1116	34-35	0.12795085215454804
1116	36-37	0.08622424314305732
1116	38-39	0.20645560326872214
1116	40-41	0.1223663827871917
1116	42-43	0.22262224099418404
1116	44-45	0.1087378843946496
1116	46-47	0.09596217211481672
1116	48-49	0.08914248420128246
1116	50-51	0.2326191104280113
1116	52-53	0.20296928563163874
1116	54-55	0.21242293594438877
1116	56-57	0.022427604360260034
1116	58-59	0.1147365581669817
1116	60-61	0.15386759245128445
1116	62-63	0.20107542801331135
1116	64-65	0.18915307789627178
1116	66-67	0.19112506591641676
1116	68-69	0.1267281414997683
1116	70-71	0.24351679659960013
1116	72-73	0.33252458825417364
1116	74-75	0.05775676548588393
1116	76	-0.3063901512936589
2118	1	0.02802665799206494
2118	2	0.03168055088239896
2118	3	0.10428379637361473
2118	4	0.011825781784999378
2118	5	0.04243206607067407
2118	6	0.05610436944274255
2118	7	0.14068605601613626
2118	8	0.13920728986317954
2118	9	0.15292096750333428
2118	10-11	0.15572574116059457
2118	12-13	0.12227791021551582
2118	14-15	-0.0025286107996222995
2118	16-17	-0.08187197122381207
2118	18-19	0.05763313475272014
2118	20-21	0.01765051572413512
2118	22-23	0.2290555456700787
2118	24-25	0.033333647981059755
2118	26-27	0.07209496808523852
2118	28-29	-0.03460025577047077
2118	30-31	0.15942711581143243
2118	32-33	0.03403840346506826
2118	34-35	0.16548466531069295
2118	36-37	0.12042564960082558
2118	38-39	0.02674939866874837
2118	40-41	0.10402092903414939
2118	42-43	0.17136197448869694
2118	44-45	-0.10241194245690366
2118	46-47	0.07155879932390263
2118	48-49	-0.1803116527799915
2118	50-51	0.054153325278690545
2118	52-53	-0.025788194812385257
2118	54-55	0.0315733995281704
2118	56-57	-0.04625626301090335
2118	58-59	-0.07608033183417007
2118	60-61	-0.18865710927161672
2118	62-63	-0.0447471692689021
2118	64-65	-0.4270821393981947
2118	66-67	-0.07138390477482659
2118	68-69	-0.34370302552938625
2118	70-71	0.0439144707572936
2118	72-73	-0.14474708342108045
2118	74-75	-0.26959897053631465
2118	76	-0.6906669599513862
1119	1	-0.026413279722035554
1119	2	0.01741580376823748
1119	3	0.016465933172597147
1119	4	0.011991781358268838
1119	5	0.036534850303205246
1119	6	0.014404004521956892
1119	7	0.046191750176312496
1119	8	0.1337008517432281
1119	9	0.09322268237603737
1119	10-11	0.1314927707830904
1119	12-13	0.040314044966279994
1119	14-15	-0.07671529949659117
1119	16-17	0.026583023668131034
1119	18-19	0.018760839195564927
1119	20-21	0.0619887717427261
1119	22-23	0.2459252736005837
1119	24-25	0.14651831032470142
1119	26-27	0.16806089450057726
1119	28-29	0.14725876510237157
1119	30-31	0.2223144043728098
1119	32-33	0.21730179038224406
1119	34-35	0.21933295623917104
1119	36-37	0.15950601253279473
1119	38-39	0.18740599200653918
1119	40-41	0.44879247347021334
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2101	38-39	0.19574097047323136
2101	40-41	-0.17523877035110047
2101	42-43	-0.038990860371178826
2101	44-45	0.1434637300024164
2101	46-47	0.05089438479594577
2101	48-49	0.16621500045108206
2101	50-51	0.14195705066345
2101	52-53	0.18935756935616865
2101	54-55	0.14076481915715533
2101	56-57	0.19828967657930718
2101	58-59	0.07422833962093023
2101	60-61	0.029302438029802147
2101	62-63	-0.047763332614280785
2101	64-65	0.14685735742779826
2101	66-67	0.07537853736875633
2101	68-69	-0.009703213991855364
2101	70-71	0.14656097511768706
2101	72-73	0.5467639351861635
2101	74-75	0.2524387939443855
2101	76	0.2446152182885193
2107	1	0.07242711669485402
2107	2	0.007231406971222043
2107	3	0.06783432464791161
2107	4	-0.04109589489129917
2107	5	-0.004263450649943934
2107	6	-0.09055575281069395
2107	7	-0.2882816429693662
2107	8	-0.23299902662392924
2107	9	-0.13105525644149907
2107	10-11	-0.009540260731462524
2107	12-13	-0.3967505354150802
2107	14-15	0.054133863166732965
2107	16-17	-0.1353564555602773
2107	18-19	-0.023879669908424717
2107	20-21	-0.015758357478752316
2107	22-23	0.23850765000774743
2107	24-25	0.017536521227889068
2107	26-27	0.07761674251202777
2107	28-29	-0.0024624061981413092
2107	30-31	0.009339550137177355
2107	32-33	-0.04995254837886165
2107	34-35	-0.07205291904067934
2107	36-37	-0.2771778896083461
2107	38-39	-0.15345773022827558
2107	40-41	-0.05679309026295698
2107	42-43	-0.16054058501824642
2107	44-45	-0.04052210357893671
2107	46-47	-0.1419648861665621
2107	48-49	-0.015698340303259783
2107	50-51	-0.27796676767336237
2107	52-53	-0.034955421198603176
2107	54-55	-0.227520484296015
2107	56-57	0.04336294359480064
2107	58-59	-0.049574122064939274
2107	60-61	6.072615479268961E-5
2107	62-63	-0.29827212563452576
2107	64-65	0.05135767130079927
2107	66-67	-0.284414710432479
2107	68-69	0.012045754519533602
2107	70-71	-0.2248240490532183
2107	72-73	0.22621151871125988
2107	74-75	-0.017287106085248638
2107	76	0.30285347708714383
2106	1	0.041528899787060425
2106	2	0.038572405368356044
2106	3	-0.038400969362811566
2106	4	-0.016651939194893828
2106	5	0.011626939725431384
2106	6	-0.04860054050975293
2106	7	-0.5859233528887202
2106	8	-0.16058244122172027
2106	9	-0.06274148868184426
2106	10-11	0.02355877648538751
2106	12-13	0.09902277354952105
2106	14-15	0.021764100634662498
2106	16-17	-0.07876879339867315
2106	18-19	-0.1680247056875004
2106	20-21	-0.18655057766014949
2106	22-23	0.06500536233367171
2106	24-25	0.009129186493147756
2106	26-27	-0.029016770072679776
2106	28-29	0.0054068411793579685
2106	30-31	-0.10549244827782189
2106	32-33	-0.06623122079781041
2106	34-35	-0.10102322184128809
2106	36-37	-0.31728436195525234
2106	38-39	-0.48994871419017016
2106	40-41	-0.1181720231825949
2106	42-43	-0.23649892247917847
2106	44-45	-0.06271684382197407
2106	46-47	-0.1835618532224217
2106	48-49	-0.11068130127421227
2106	50-51	-0.36310126376251617
2106	52-53	-0.08765387082721077
2106	54-55	-0.45584627586335813
2106	56-57	-0.02780172985839613
2106	58-59	-0.002633104158277888
2106	60-61	0.03140873768239061
2106	62-63	-0.27032740606735217
2106	64-65	-0.08631636566612855
2106	66-67	-0.2708335788131393
2106	68-69	0.050733893180179734
2106	70-71	-0.2355084397030609
2106	72-73	0.08768004702325527
2106	74-75	0.009821036386419735
2106	76	0.104731390452109
2105	1	0.02876850134958886
2105	2	2.5813937674712406E-4
2105	3	-0.05115274590944807
2105	4	-0.03698027767995882
2105	5	-0.042311880663639556
2105	6	-0.10268834476075739
2105	7	-0.0870648188838885
2105	8	-0.043538283454140014
2105	9	-0.1753391926421699
2105	10-11	-0.09461634123205442
2105	12-13	0.1318905820560019
2105	14-15	-0.03677877931443163
2105	16-17	-0.01685137772785339
2105	18-19	-0.08861481502815138
2105	20-21	-0.08896268125138107
2105	22-23	0.3340604796700717
2105	24-25	0.13580814146450138
2105	26-27	0.11914847949879714
2105	28-29	0.06456241801430451
2105	30-31	-0.011276962264552992
2105	32-33	-0.09107203386262341
2105	34-35	-0.020843450593929447
2105	36-37	-0.17213921604059834
2105	38-39	-0.1425375062506049
2105	40-41	-0.0824419277973476
2105	42-43	-0.4060504406584968
2105	44-45	-0.25561181490216
2105	46-47	-0.25168122709950325
2105	48-49	-0.22844288476947838
2105	50-51	-0.4239813544238089
2105	52-53	-0.1718183725250313
2105	54-55	-0.7984735553209674
2105	56-57	-0.33830506499268154
2105	58-59	-0.17304859006303275
2105	60-61	-0.09985326475839429
2105	62-63	-0.9155398690527576
2105	64-65	-0.923013879408245
2105	66-67	-0.918410580287123
2105	68-69	-0.1284081219631119
2105	70-71	-1.2628751954646162
2105	72-73	-0.6191934961861989
2105	74-75	-0.6749684378404268
2105	76	-0.013946374457145083
2104	1	2.4266839488973346E-4
2104	2	-0.009290667713784728
2104	3	-0.08911841408157528
2104	4	-0.05697738380110451
2104	5	-0.049759375390948435
2104	6	-0.19323331788313425
2104	7	0.0320624443138513
2104	8	0.12840651207508103
2104	9	0.06885347324080016
2104	10-11	-0.03829788057818462
2104	12-13	0.13349505069127332
2104	14-15	-0.06674456979025933
2104	16-17	-0.03780635895224549
2104	18-19	-0.0859709154276942
2104	20-21	0.010284151868290792
2104	22-23	0.3151373529013881
2104	24-25	0.18431346026095952
2104	26-27	0.04883771133827963
2104	28-29	0.09427430191919939
2104	30-31	-0.15286782461335946
2104	32-33	-0.09900916470572696
2104	34-35	-0.07770803457867714
2104	36-37	-0.20840020117883995
2104	38-39	-0.19236366887756873
2104	40-41	0.11531501214003725
2104	42-43	-0.19030709268262314
2104	44-45	-0.1827715661032201
2104	46-47	-0.008720822139430595
2104	48-49	-0.13473763704399744
2104	50-51	0.001174093757548178
2104	52-53	-0.24306399356208885
2104	54-55	-0.12542194743360824
2104	56-57	-0.277075135784969
2104	58-59	-0.0698748120963657
2104	60-61	-0.13970556679146995
2104	62-63	-0.11564555762529238
2104	64-65	-0.6998477743133762
2104	66-67	-0.08508035169416672
2104	68-69	-0.46389518711312405
2104	70-71	-0.08192888985788471
2104	72-73	-0.7444335217086717
2104	74-75	0.18893305074364974
2104	76	-0.23554108467773105
2111	1	0.03729163334581642
2111	2	-0.03350977765976637
2111	3	-0.01049852678706742
2111	4	-0.05346250517835216
2111	5	-0.04745332808796121
2111	6	-0.13213139562064669
2111	7	-0.013256661658530788
2111	8	-0.0017759086866746543
2111	9	-0.014182871613954262
2111	10-11	-0.022695486432169787
2111	12-13	0.03223497617370441
2111	14-15	-0.16286271660342067
2111	16-17	-0.11148630744020949
2111	18-19	-0.07368588684657595
2111	20-21	1.8466266060102043E-4
2111	22-23	0.3119500821312329
2111	24-25	0.008898156226599951
2111	26-27	-0.01632661920087486
2111	28-29	0.029075169139048285
2111	30-31	-0.13218965065563282
2111	32-33	-0.00623622820459957
2111	34-35	-0.11258578875283831
2111	36-37	-0.14364735248394567
2111	38-39	-0.06409482481568318
2111	40-41	-0.4369248207973868
2111	42-43	-0.21927393143524654
2111	44-45	-0.15094886077257996
2111	46-47	0.04643442229681938
2111	48-49	-0.23618780920133986
2111	50-51	0.06420737790588049
2111	52-53	-0.1517380680639917
2111	54-55	0.03599609026065309
2111	56-57	-0.18220218833798185
2111	58-59	0.08006337551932319
2111	60-61	-0.2710218522460721
2111	62-63	-0.010963755135094289
2111	64-65	-0.3190531457940651
2111	66-67	0.08517333389417558
2111	68-69	-0.1496234764456048
2111	70-71	0.10574506041361076
2111	72-73	-0.3252585209442316
2111	74-75	0.5524935977060679
2111	76	0.08886918647397479
2110	1	0.020499279553533256
2110	2	0.0112873340250772
2110	3	0.06425040487358302
2110	4	0.008793843321917905
2110	5	0.009452307189121711
2110	6	0.06234744826937799
2110	7	0.030923819427997046
2110	8	0.08479936662305221
2110	9	0.09408047858767787
2110	10-11	-0.04020270875571441
2110	12-13	-0.031259854744291715
2110	14-15	-0.13090562597312072
2110	16-17	-0.13676990237816966
2110	18-19	-0.21311278982443582
2110	20-21	-0.08444501309279673
2110	22-23	0.1955742852551623
2110	24-25	-0.10792561264383949
2110	26-27	-0.09446111054352713
2110	28-29	-0.05445057667289177
2110	30-31	-0.33646961867309955
2110	32-33	-0.05037485177079759
2110	34-35	-0.015585694712356712
2110	36-37	0.010126749411178082
2110	38-39	0.07259906873955657
2110	40-41	-0.44950400017646075
2110	42-43	-0.11205391868493564
2110	44-45	-0.010231102288130955
2110	46-47	0.15959595064330045
2110	48-49	0.0318542372774715
2110	50-51	0.16863517814401519
2110	52-53	-0.016832216040420178
2110	54-55	0.19293360862450015
2110	56-57	-0.06228413664614152
2110	58-59	0.11321614040451777
2110	60-61	0.028096039269833284
2110	62-63	0.1527168020759575
2110	64-65	0.16758504742358582
2110	66-67	0.22361661439470737
2110	68-69	0.0823844954961146
2110	70-71	0.21764224886648975
2110	72-73	0.05611331760274396
2110	74-75	0.5813574000332693
2110	76	0.015088418381161262
2109	1	0.014693253607440226
2109	2	-0.06213700155889512
2109	3	0.005726320867111667
2109	4	-0.010921574390863498
2109	5	0.023575627374356145
2109	6	0.014497251203891892
2109	7	0.006515612457846487
2109	8	0.146654782675256
2109	9	0.01551299181296173
2109	10-11	0.003298038997051833
2109	12-13	0.08430778705269404
2109	14-15	-0.11939606038271933
2109	16-17	-0.06734189699012205
2109	18-19	-0.06431112986702203
2109	20-21	-0.1956553620165451
2109	22-23	0.0743153819472866
2109	24-25	-0.12710904050339167
2109	26-27	-0.26954268501739165
2109	28-29	-0.1252452249783289
2109	30-31	-0.7658854897985705
2109	32-33	-0.09956070752289037
2109	34-35	-0.14319962986797918
2109	36-37	-0.13881021557637752
2109	38-39	-0.2130620186084684
2109	40-41	-0.5774081037366514
2109	42-43	-0.16813843869745426
2109	44-45	-0.15744402956212156
2109	46-47	0.037916759606467565
2109	48-49	-0.012219032613643321
2109	50-51	0.08595327441971534
2109	52-53	-0.037987815467275254
2109	54-55	-0.025840223593561973
2109	56-57	0.04157484601604011
2109	58-59	0.11235756664751761
2109	60-61	-0.17639698042354723
2109	62-63	0.07691784660131873
2109	64-65	0.2058841567360119
2109	66-67	0.1665182383497381
2109	68-69	-0.010743456347725555
2109	70-71	0.12184111626213223
2109	72-73	-0.6658506336820125
2109	74-75	0.6645111596468318
2109	76	-0.06818095791450673
2108	1	0.02316761517447219
2108	2	-0.040312065224625826
2108	3	-0.09592139877115358
2108	4	0.006652834996145884
2108	5	0.008193381934333388
2108	6	-0.060997472989718915
2108	7	-0.16971900948153262
2108	8	0.0032283112154587457
2108	9	-0.014164634994827452
2108	10-11	0.016675899882976353
2108	12-13	0.10642984518496235
2108	14-15	-0.07810158768513276
2108	16-17	0.029943125718723707
2108	18-19	0.008495247156218966
2108	20-21	0.03256980631477546
2108	22-23	0.14177577211673054
2108	24-25	0.04545725260641831
2108	26-27	-0.022669985251333458
2108	28-29	-0.08151357878105614
2108	30-31	-0.021044771738765178
2108	32-33	0.00728234566314967
2108	34-35	-0.2460858282607461
2108	36-37	-0.47722589247796776
2108	38-39	-0.5106745426032901
2108	40-41	-0.775441648301296
2108	42-43	-0.17568244258276877
2108	44-45	-0.3338630095261337
2108	46-47	-0.29813079322737934
2108	48-49	-0.32433586764555855
2108	50-51	-0.1693909876120756
2108	52-53	-0.16393573240254966
2108	54-55	-0.2703539606415646
2108	56-57	-0.30868772844537773
2108	58-59	-0.11976601045914492
2108	60-61	-0.6630770848674601
2108	62-63	-0.17233202547909343
2108	64-65	-0.0014314185085311237
2108	66-67	-0.15134055742816344
2108	68-69	-0.6370140281019019
2108	70-71	-0.2136888485899533
2108	72-73	-1.9466671237042874
2108	74-75	-0.021699267281114487
2108	76	-0.3971258916428866
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	1.0
16	4.0
17	12.0
18	33.0
19	63.0
20	151.0
21	310.0
22	501.0
23	699.0
24	1086.0
25	1800.0
26	2897.0
27	5202.0
28	7506.0
29	11087.0
30	16619.0
31	25057.0
32	36892.0
33	55815.0
34	89744.0
35	191335.0
36	531335.0
37	1244398.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	69.91680727092754	7.1322579919461875	6.47575982542575	16.475174911700524
2	12.909720816179613	62.37596454522958	13.80922813884952	10.905086499741289
3	12.57757980697847	11.666014262896223	63.26035409777057	12.496051832354729
4	13.760410339474927	14.209984027355965	9.094058626352613	62.93554700681649
5	65.883872128861	14.723313311286585	10.385548131650582	9.00726642820184
6	62.701941463634114	16.829812602641113	11.270162651008976	9.1980832827158
7	8.849879642752693	62.29677622550673	18.519943308362016	10.33340082337855
8	10.208859193268992	63.59748937031788	13.765719556365436	12.427931880047694
9	62.46721108636476	11.054509459854671	14.984679759735439	11.493599694045129
10-11	37.52448763807338	41.15983892375875	10.872398821173878	10.443274616993993
12-13	10.6612674630492	38.51308182043149	13.559424984814738	37.26622573170457
14-15	10.734944095745877	38.69717216710535	13.061708398011293	37.50617533913748
16-17	37.544104744550175	12.6734156711885	12.499538818024341	37.28294076623698
18-19	11.035634743875278	12.810217992846054	39.03457739983352	37.11956986344514
20-21	37.186025061303454	12.854738926008412	38.95370182898022	11.005534183707903
22-23	11.00679399788531	64.78670896042834	12.624800341949562	11.581696699736789
24-25	11.096960698297002	12.863737598704192	39.008998672695775	37.03030303030303
26-27	11.174416773525905	12.885446896582753	12.446379159074036	63.4937571708173
28-29	37.2668106454298	38.8635126318868	12.579132078018493	11.29054464466491
30-31	10.855796270050169	13.032395221704798	38.72788013767969	37.383928370565336
32-33	10.725607972824008	38.98481473982587	12.822321207621876	37.46725607972824
34-35	11.435580751839105	38.84135340037345	12.669523745247577	37.05354210253988
36-37	36.929585386155544	38.77210861397944	12.929180445884231	11.369125553980789
38-39	11.051134957593755	13.555803019054691	38.43999010146003	36.95307192189152
40-41	10.964162785989066	38.87017164968167	38.30917189714517	11.85649366718409
42-43	10.705675912802862	12.78299700794133	39.184675260399096	37.32665181885672
44-45	37.029425659715194	12.725675462869226	13.11603788441205	37.128860993003535
46-47	11.147848192391622	38.87622325706958	38.747204787293875	11.228723763244922
48-49	37.216553058423884	12.723695754876156	38.857191064318016	11.20256012238195
50-51	11.36498616454073	12.71303232773166	12.738161121234617	63.183820386493
52-53	37.104384603271015	12.698566961373198	39.00485928325572	11.192189152100065
54-55	10.876155767024363	12.636363636363637	39.35639243211626	37.131088164495736
56-57	10.835639243211627	12.67179591010326	39.21119884816989	37.28136599851522
58-59	37.29621380846325	12.635463769094057	38.73080470630582	11.33751771613687
60-61	11.268790353422869	12.933657285550382	38.92213898449979	36.875413376526964
62-63	37.190569391014826	13.00155227104002	38.9992576095026	10.808620728442554
64-65	11.154304740050842	65.3462014352883	12.611054869406763	10.8884389552541
66-67	10.950147353265393	65.51629884592023	12.694697532114013	10.838856268700367
68-69	11.005736653843558	65.4125891431014	12.633821511327078	10.94785269172797
70-71	11.150165350610784	65.48181143281366	12.419315650941485	10.94870756563407
72-73	11.032687678567411	65.25713707228184	12.687116150367823	11.02305909878293
74-75	11.708285527884637	57.77406582529076	16.210478954354233	14.307169692470362
76	12.191761715147017	55.27304222627163	17.346606375559606	15.188589683021755
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	238.0
1	176.5
2	118.5
3	105.0
4	88.0
5	76.0
6	56.5
7	54.0
8	59.0
9	54.5
10	62.5
11	68.0
12	61.0
13	86.0
14	114.0
15	130.0
16	187.5
17	232.0
18	295.5
19	476.0
20	708.5
21	824.0
22	1005.0
23	1485.5
24	2245.0
25	2705.0
26	3250.5
27	4426.5
28	5901.5
29	6746.0
30	7774.0
31	10399.0
32	13674.5
33	15353.0
34	17523.5
35	22259.0
36	28296.0
37	31768.0
38	36157.0
39	46363.5
40	56932.5
41	79234.0
42	96784.0
43	503623.0
44	606434.0
45	214188.5
46	125971.0
47	102032.5
48	70596.5
49	58465.5
50	53832.0
51	49668.0
52	41001.5
53	34493.0
54	32487.0
55	30144.5
56	25067.5
57	19004.5
58	15676.0
59	15662.5
60	15524.5
61	16351.5
62	17303.0
63	17203.0
64	17012.0
65	15237.5
66	11139.5
67	8725.0
68	5811.0
69	2809.5
70	3194.5
71	3667.0
72	3228.5
73	2543.0
74	1486.0
75	676.0
76	476.5
77	182.5
78	63.0
79	38.0
80	28.5
81	20.0
82	17.5
83	14.0
84	9.5
85	6.0
86	5.0
87	3.0
88	2.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	2222550.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.82389500096882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.75692448732588	23.659473809946252
2	16.178523119471507	9.973701958106727
3	3.972234456732524	3.6731921344066123
4	1.279757084004887	1.5778839193645071
5	0.4811924041001333	0.7416112069623133
6	0.3156122210697953	0.5837038777966758
7	0.1357922572397674	0.2929952395372197
8	0.1265861102833656	0.3121501577564417
9	0.10861596726873732	0.3013170453468203
>10	0.5164435066077109	2.8874094912732513
>50	0.05941819301752754	1.272761301936773
>100	0.059775200535813484	3.622233185800227
>500	0.0052545322193240426	1.0757595187954545
>1k	0.003423885388320498	2.3332461097563604
>5k	1.4885824491786561E-4	0.41144101302315683
>10k+	2.9771648983573123E-4	47.281120030191246
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC	1019787	45.8836471620436	TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCACCTCGTATGC	10664	0.4798092281388495	TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAACATCTCGTATGC	8967	0.4034554903151785	TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATTATCTCGTATGC	4979	0.2240219567613777	TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAACTCGTATGC	4890	0.22001754741175678	TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCTCATCTCGTATGC	3099	0.1394344334210704	TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGGACTCCAGTCACCAGATCATCTCGTATGC	2874	0.12931092663832083	TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATATCGTATGC	2833	0.1274661987356865	TruSeq Adapter, Index 7 (98% over 50bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC	2805	0.12620638455827765	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGATCATCTCGTATGC	2656	0.1195023733999235	TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAACATCTCGTATGC	2332	0.10492452363276415	TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAGCTCGTATGC	2329	0.1047895435423275	TruSeq Adapter, Index 7 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC	2299	0.1034397426379609	TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGATCATCTCGTATGC	2263	0.10181998155272098	TruSeq Adapter, Index 7 (98% over 50bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.017097478121977008	0.0	0.0
2	0.018717239207216935	0.0	0.0
3	0.021326854288992374	0.0	0.0
4	0.02339654900902117	0.0	0.0
5	0.02758093181255765	0.0	8.998672695777372E-5
6	0.029965580076938652	0.0	8.998672695777372E-5
7	0.03190029470653079	0.0	8.998672695777372E-5
8	0.03738948505095498	0.0	8.998672695777372E-5
9	0.04103394749274482	0.0	8.998672695777372E-5
10	0.05678162471035522	4.499336347888686E-5	8.998672695777372E-5
11	0.06182088141999055	4.499336347888686E-5	8.998672695777372E-5
12	0.06573530404265371	4.499336347888686E-5	8.998672695777372E-5
13	0.07801849227238983	4.499336347888686E-5	8.998672695777372E-5
14	0.09057164068299926	4.499336347888686E-5	8.998672695777372E-5
15	0.09363118939956357	4.499336347888686E-5	8.998672695777372E-5
16	0.11315830914940046	4.499336347888686E-5	8.998672695777372E-5
17	0.11572293086769701	4.499336347888686E-5	8.998672695777372E-5
18	0.11756765877033137	8.998672695777372E-5	8.998672695777372E-5
19	0.1325954421722796	8.998672695777372E-5	8.998672695777372E-5
20	0.13534003734449168	8.998672695777372E-5	8.998672695777372E-5
21	0.13763469888191493	8.998672695777372E-5	8.998672695777372E-5
22	0.13992936041933815	8.998672695777372E-5	8.998672695777372E-5
23	0.14253897550111358	8.998672695777372E-5	8.998672695777372E-5
24	0.14379878967852242	8.998672695777372E-5	8.998672695777372E-5
25	0.14609345121594564	8.998672695777372E-5	8.998672695777372E-5
26	0.1477132123011856	8.998672695777372E-5	8.998672695777372E-5
27	0.14951294684034105	8.998672695777372E-5	8.998672695777372E-5
28	0.15423725000562416	8.998672695777372E-5	8.998672695777372E-5
29	0.15981642707700613	8.998672695777372E-5	8.998672695777372E-5
30	0.19315650941486132	8.998672695777372E-5	8.998672695777372E-5
31	0.19549616431576342	8.998672695777372E-5	8.998672695777372E-5
32	0.1994105869384266	8.998672695777372E-5	8.998672695777372E-5
33	0.20161526174889205	8.998672695777372E-5	8.998672695777372E-5
34	0.20498976400980856	8.998672695777372E-5	8.998672695777372E-5
35	0.207599379091584	8.998672695777372E-5	8.998672695777372E-5
36	0.21083890126206384	8.998672695777372E-5	8.998672695777372E-5
37	0.2133585296168815	8.998672695777372E-5	8.998672695777372E-5
38	0.21556320442734697	8.998672695777372E-5	8.998672695777372E-5
39	0.21907268677870015	8.998672695777372E-5	8.998672695777372E-5
40	0.22177228858743336	8.998672695777372E-5	8.998672695777372E-5
41	0.22415693685181437	8.998672695777372E-5	8.998672695777372E-5
42	0.22627162493532205	8.998672695777372E-5	8.998672695777372E-5
43	0.2301410541945063	8.998672695777372E-5	8.998672695777372E-5
44	0.23279566263976065	8.998672695777372E-5	8.998672695777372E-5
45	0.23675507862590267	8.998672695777372E-5	8.998672695777372E-5
46	0.24143438842770693	8.998672695777372E-5	8.998672695777372E-5
47	0.2455737778677645	8.998672695777372E-5	8.998672695777372E-5
48	0.25128793502958313	8.998672695777372E-5	8.998672695777372E-5
49	0.25529234437920406	8.998672695777372E-5	8.998672695777372E-5
50	0.2598816674540505	8.998672695777372E-5	8.998672695777372E-5
51	0.26631571843153135	8.998672695777372E-5	8.998672695777372E-5
52	0.2729297428629277	8.998672695777372E-5	8.998672695777372E-5
53	0.27738408584733754	8.998672695777372E-5	8.998672695777372E-5
54	0.2844030505500439	8.998672695777372E-5	8.998672695777372E-5
55	0.2907471148005669	8.998672695777372E-5	8.998672695777372E-5
56	0.29785606623023103	1.349800904366606E-4	8.998672695777372E-5
57	0.3080245663764595	1.349800904366606E-4	8.998672695777372E-5
58	0.31837303997660343	1.349800904366606E-4	8.998672695777372E-5
59	0.3287665069402263	1.349800904366606E-4	8.998672695777372E-5
60	0.3396549009021169	1.349800904366606E-4	8.998672695777372E-5
61	0.34986839441182427	1.349800904366606E-4	8.998672695777372E-5
62	0.3627364963667859	1.349800904366606E-4	8.998672695777372E-5
63	0.38005894130615736	1.349800904366606E-4	8.998672695777372E-5
64	0.3956266450698522	1.349800904366606E-4	8.998672695777372E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGAAG	117505	0.0	68.84728	3
GATCGGA	117805	0.0	68.75812	1
GCACACG	117620	0.0	68.74723	11
ACGTCTG	117500	0.0	68.73702	15
CGGAAGA	117710	0.0	68.72738	4
AGCACAC	117745	0.0	68.70398	10
ACACGTC	117640	0.0	68.69687	13
CACACGT	117685	0.0	68.688446	12
GAGCACA	117885	0.0	68.663956	9
ATCGGAA	117920	0.0	68.65248	2
CACGTCT	117670	0.0	68.64962	14
CGTCTGA	114480	0.0	68.59058	16
AGAGCAC	118015	0.0	68.58239	8
TCTCGTA	114260	0.0	68.54805	41
CATCTCG	112535	0.0	68.54446	39
CTGAACT	114495	0.0	68.5388	19
ATGCCGT	116725	0.0	68.53373	47
TATGCCG	116865	0.0	68.526505	46
CGTATGC	116985	0.0	68.525024	44
TCTGAAC	114645	0.0	68.51629	18
>>END_MODULE