FastQCFastQC Report
Mon 15 Sep 2014
TGA_6_CAGATC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameTGA_6_CAGATC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2222550
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC101978745.8836471620436TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCACCTCGTATGC106640.4798092281388495TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAACATCTCGTATGC89670.4034554903151785TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATTATCTCGTATGC49790.2240219567613777TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAACTCGTATGC48900.22001754741175678TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCTCATCTCGTATGC30990.1394344334210704TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGGACTCCAGTCACCAGATCATCTCGTATGC28740.12931092663832083TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATATCGTATGC28330.1274661987356865TruSeq Adapter, Index 7 (98% over 50bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC28050.12620638455827765No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGATCATCTCGTATGC26560.1195023733999235TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAACATCTCGTATGC23320.10492452363276415TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAGCTCGTATGC23290.1047895435423275TruSeq Adapter, Index 7 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC22990.1034397426379609TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGATCATCTCGTATGC22630.10181998155272098TruSeq Adapter, Index 7 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG1175050.068.847283
GATCGGA1178050.068.758121
GCACACG1176200.068.7472311
ACGTCTG1175000.068.7370215
CGGAAGA1177100.068.727384
AGCACAC1177450.068.7039810
ACACGTC1176400.068.6968713
CACACGT1176850.068.68844612
GAGCACA1178850.068.6639569
ATCGGAA1179200.068.652482
CACGTCT1176700.068.6496214
CGTCTGA1144800.068.5905816
AGAGCAC1180150.068.582398
TCTCGTA1142600.068.5480541
CATCTCG1125350.068.5444639
CTGAACT1144950.068.538819
ATGCCGT1167250.068.5337347
TATGCCG1168650.068.52650546
CGTATGC1169850.068.52502444
TCTGAAC1146450.068.5162918
Produced by FastQC (version 0.11.2)