##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	TGA3_5_GCCAAT_L001_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2647010
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.24273274373727	34.0	34.0	34.0	34.0	34.0
2	33.276161782539546	34.0	34.0	34.0	34.0	34.0
3	33.28548966569828	34.0	34.0	34.0	34.0	34.0
4	33.30117944397641	34.0	34.0	34.0	34.0	34.0
5	33.46938659090823	34.0	34.0	34.0	34.0	34.0
6	37.00128635705948	38.0	38.0	38.0	36.0	38.0
7	36.62608943676072	38.0	38.0	38.0	34.0	38.0
8	36.682617368275906	38.0	38.0	38.0	34.0	38.0
9	36.83514002591603	38.0	38.0	38.0	35.0	38.0
10-11	36.967577190868184	38.0	38.0	38.0	36.0	38.0
12-13	36.83079984586382	38.0	38.0	38.0	35.5	38.0
14-15	36.70158896264087	38.0	37.5	38.0	34.0	38.0
16-17	36.79354290312466	38.0	37.5	38.0	35.0	38.0
18-19	36.92191661535091	38.0	37.5	38.0	36.0	38.0
20-21	36.962857337146446	38.0	38.0	38.0	36.0	38.0
22-23	37.12511154094619	38.0	38.0	38.0	36.5	38.0
24-25	36.88107317312742	38.0	38.0	38.0	36.0	38.0
26-27	37.002266141797726	38.0	38.0	38.0	36.0	38.0
28-29	36.89451664330698	38.0	38.0	38.0	35.5	38.0
30-31	36.985673646869486	38.0	38.0	38.0	36.0	38.0
32-33	37.06295027219391	38.0	38.0	38.0	36.0	38.0
34-35	36.96814235684791	38.0	38.0	38.0	36.0	38.0
36-37	36.79308257241189	38.0	38.0	38.0	35.5	38.0
38-39	36.81725342934103	38.0	38.0	38.0	36.0	38.0
40-41	36.99038726714292	38.0	38.0	38.0	36.0	38.0
42-43	36.74683000819793	38.0	37.5	38.0	34.5	38.0
44-45	36.68717609680357	38.0	37.5	38.0	34.5	38.0
46-47	36.60950053078757	38.0	37.5	38.0	34.0	38.0
48-49	36.368386216901335	38.0	37.0	38.0	34.0	38.0
50-51	36.459715112523185	38.0	37.5	38.0	33.0	38.0
52-53	36.362962361305776	38.0	37.0	38.0	34.0	38.0
54-55	36.649950887983046	38.0	37.5	38.0	34.5	38.0
56-57	36.50860026973831	38.0	37.0	38.0	34.0	38.0
58-59	36.690701206266695	38.0	37.5	38.0	35.0	38.0
60-61	36.7031104907046	38.0	38.0	38.0	35.5	38.0
62-63	36.675524081888625	38.0	38.0	38.0	35.0	38.0
64-65	36.394519476692565	38.0	37.5	38.0	34.0	38.0
66-67	36.14176920374309	38.0	37.0	38.0	33.5	38.0
68-69	28.96655754983925	37.0	21.0	38.0	10.0	38.0
70-71	27.836675909800114	37.0	15.0	38.0	11.0	38.0
72-73	28.028260187910135	37.0	15.0	38.0	11.0	38.0
74-75	28.15863219254933	37.0	11.0	38.0	11.0	38.0
76	27.983290580692934	35.0	11.0	38.0	11.0	38.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.12323144758326521
1101	2	0.18511401248107973
1101	3	0.169112049158187
1101	4	0.1103461112664661
1101	5	0.08541918682377769
1101	6	0.23248866460629358
1101	7	0.2509674378707558
1101	8	0.4251901822187065
1101	9	0.27478840057128195
1101	10-11	0.29773278951728344
1101	12-13	0.24605298609566972
1101	14-15	0.41296787598793827
1101	16-17	0.13222302618914483
1101	18-19	0.05939039884498243
1101	20-21	0.08910550974450615
1101	22-23	0.16047723445969808
1101	24-25	0.06159895127974835
1101	26-27	0.08514555115394273
1101	28-29	0.23949642497957058
1101	30-31	0.05590493337835056
1101	32-33	0.13019314558170691
1101	34-35	0.12564226053145688
1101	36-37	0.19188557283179364
1101	38-39	0.2439864356349517
1101	40-41	0.1501329931081372
1101	42-43	0.15511463347851873
1101	44-45	0.20337328063069293
1101	46-47	0.23851865890839008
1101	48-49	0.27884320659653383
1101	50-51	0.20193597988640732
1101	52-53	0.04597867256531174
1101	54-55	0.15897587746255937
1101	56-57	0.3074755754061229
1101	58-59	0.11253335161327271
1101	60-61	0.3303828870224237
1101	62-63	0.25925320978608113
1101	64-65	0.4930869613345692
1101	66-67	0.17957492173403722
1101	68-69	0.010006514092612662
1101	70-71	0.00923097225133418
1101	72-73	-0.042718618198552605
1101	74-75	-0.08225151537519082
1101	76	-0.08090856176980665
1102	1	0.12950914193037733
1102	2	0.2127156588462853
1102	3	0.17731142991722493
1102	4	0.06068137895531578
1102	5	0.16640533733270502
1102	6	0.2706890959737578
1102	7	0.07205500299303225
1102	8	0.4703428603431803
1102	9	0.3593342596290725
1102	10-11	0.34683513731811644
1102	12-13	0.3707271429824104
1102	14-15	0.3338701941245219
1102	16-17	0.13158802609644482
1102	18-19	0.1446712202971696
1102	20-21	0.2541258112073237
1102	22-23	0.18487746064950983
1102	24-25	0.17445345437285908
1102	26-27	0.13669844310813062
1102	28-29	0.3584849362674234
1102	30-31	0.07220543210835473
1102	32-33	0.22183231102781065
1102	34-35	0.23831058963145324
1102	36-37	0.29576355393320597
1102	38-39	0.23153193624987267
1102	40-41	0.14602356915056447
1102	42-43	0.16347067849400077
1102	44-45	0.2069255731200741
1102	46-47	0.25163535060426057
1102	48-49	0.2766043291407314
1102	50-51	0.16373832954125334
1102	52-53	0.18048096227366983
1102	54-55	0.23724187915589567
1102	56-57	0.4575821812610954
1102	58-59	0.25758112339640604
1102	60-61	0.4019404449140396
1102	62-63	0.29641863735280793
1102	64-65	0.6407462675622
1102	66-67	0.3836372228136966
1102	68-69	0.42652084253752776
1102	70-71	0.07316682951691078
1102	72-73	0.07922024875115952
1102	74-75	0.238694990115782
1102	76	0.2191371705969658
1103	1	-0.027482423261396605
1103	2	0.1324390928598973
1103	3	0.07295625884544421
1103	4	0.029783730903119476
1103	5	0.019633588666835067
1103	6	-0.008515145135078228
1103	7	-0.22644362293524267
1103	8	0.3025907931706371
1103	9	0.10213870641177891
1103	10-11	0.024331353917958154
1103	12-13	0.16422431023799078
1103	14-15	-0.19991790791718955
1103	16-17	-0.0058038712491779165
1103	18-19	-0.05584449605297692
1103	20-21	0.09910237478828066
1103	22-23	-0.017225875465477714
1103	24-25	-0.04909461120511338
1103	26-27	-0.049860386631351616
1103	28-29	0.152157678783837
1103	30-31	0.09861713224636759
1103	32-33	0.0726595501109486
1103	34-35	0.03661559963246219
1103	36-37	0.22284820690787654
1103	38-39	-0.004196241585248117
1103	40-41	0.07223358388942103
1103	42-43	0.11950128779977831
1103	44-45	0.15924354690755393
1103	46-47	0.057015447400914354
1103	48-49	0.20426699273257753
1103	50-51	0.018586283215697108
1103	52-53	0.03735231803334926
1103	54-55	-0.02587313024683624
1103	56-57	0.2504906985321398
1103	58-59	0.1379005141214691
1103	60-61	0.29954087180451694
1103	62-63	0.05019721638974062
1103	64-65	0.4781724678730086
1103	66-67	0.09373773451072509
1103	68-69	0.2412130996068491
1103	70-71	-0.08800372499047171
1103	72-73	-0.010858268752194533
1103	74-75	0.16376127800041118
1103	76	0.14957544468803974
2116	1	-0.02037401186377963
2116	2	0.10388781293804783
2116	3	-0.045981822866373534
2116	4	-0.014444646197553368
2116	5	0.039220006466180735
2116	6	0.0052605388633182315
2116	7	-0.6250223589111172
2116	8	0.23440209242071575
2116	9	0.07407212015203157
2116	10-11	-0.12396133960258737
2116	12-13	-0.05201879910482177
2116	14-15	-0.17833269179778455
2116	16-17	0.017300215226732973
2116	18-19	-0.12306173831012757
2116	20-21	-0.027151335720262182
2116	22-23	-0.09329306015218464
2116	24-25	-0.3199218198449856
2116	26-27	-0.2939268707402647
2116	28-29	-0.05266398153727181
2116	30-31	-0.06729288090857466
2116	32-33	-0.21642804584146802
2116	34-35	-0.07631446444506906
2116	36-37	0.07265500508638212
2116	38-39	-0.2642965263608801
2116	40-41	0.04377592883028569
2116	42-43	-0.31067859419589894
2116	44-45	-0.45727965279452576
2116	46-47	-0.126935771149995
2116	48-49	0.04710493203647559
2116	50-51	-0.08134436279987511
2116	52-53	0.004001617716966166
2116	54-55	-0.30051488846384444
2116	56-57	0.07501870334528604
2116	58-59	-0.1121521297553727
2116	60-61	0.09684947873653726
2116	62-63	-0.30191058974405394
2116	64-65	0.1201992330012871
2116	66-67	-0.34380291971310584
2116	68-69	0.04799758426190337
2116	70-71	-0.11929441691761866
2116	72-73	0.12692459154366986
2116	74-75	0.10350014587152145
2116	76	0.015608159499389984
1117	1	-0.12383679242918788
1117	2	0.01802281456384236
1117	3	0.006104857805951269
1117	4	0.12397789327628317
1117	5	-0.12166951060623887
1117	6	-0.30432294043276187
1117	7	-1.5477342699375
1117	8	-0.09989522045167121
1117	9	-0.16594582337676655
1117	10-11	-0.19608553210829172
1117	12-13	-0.06340700672155464
1117	14-15	-0.5512992524517202
1117	16-17	-0.17686287412721668
1117	18-19	-0.29396821106389837
1117	20-21	0.11725548014315024
1117	22-23	-0.17728174676336295
1117	24-25	0.010192202497407266
1117	26-27	-0.18800811277485252
1117	28-29	0.05459460791799131
1117	30-31	-0.08630243300799378
1117	32-33	-0.018733412003250294
1117	34-35	-0.13391976401336336
1117	36-37	0.15366995647190862
1117	38-39	-0.4535745985208024
1117	40-41	0.030030853107824385
1117	42-43	-0.4297873265847798
1117	44-45	-0.11899051329483967
1117	46-47	-0.3165857364333462
1117	48-49	0.18676608413435503
1117	50-51	-0.27958926012155416
1117	52-53	0.013720119734550451
1117	54-55	-0.4628950916764367
1117	56-57	0.2035091162553826
1117	58-59	-0.42372725758222884
1117	60-61	-0.05403037487542406
1117	62-63	-0.8183113422119987
1117	64-65	0.0017858377087591748
1117	66-67	-0.694960506533647
1117	68-69	0.30858229867155273
1117	70-71	-0.43037693950271105
1117	72-73	-0.2335052012051264
1117	74-75	-0.20292961523369968
1117	76	-0.053963319580049074
2117	1	0.027598380207699336
2117	2	0.06287631919816761
2117	3	-0.018504179442686564
2117	4	0.06625150141611869
2117	5	-0.0021498460121947005
2117	6	-0.4263611031672667
2117	7	0.06441558212605969
2117	8	0.1256131381424339
2117	9	-0.034269393773172396
2117	10-11	0.014334220881359272
2117	12-13	-0.00392738913473778
2117	14-15	-0.040572358200982706
2117	16-17	0.17107543467812292
2117	18-19	-0.09331136806653717
2117	20-21	0.15251602982497303
2117	22-23	0.0418173594080784
2117	24-25	0.10624947587110967
2117	26-27	-0.033728430535227005
2117	28-29	0.222045088588942
2117	30-31	0.19963313393145654
2117	32-33	0.13305457945806864
2117	34-35	0.09185612276235844
2117	36-37	0.27339607007690603
2117	38-39	-0.12255049284379282
2117	40-41	0.15295420423333894
2117	42-43	-0.16043119397038907
2117	44-45	0.15977282167595064
2117	46-47	-0.12167084051550603
2117	48-49	0.28269226001242487
2117	50-51	0.07609974999326852
2117	52-53	0.3367888023175425
2117	54-55	-0.275783602725852
2117	56-57	0.3893965875144829
2117	58-59	0.13838887518326715
2117	60-61	0.18853216289250696
2117	62-63	-0.3328522049246345
2117	64-65	0.3251732188187617
2117	66-67	-0.16873392678963484
2117	68-69	0.20612329792597706
2117	70-71	-0.30735244967439
2117	72-73	-0.2441418554507777
2117	74-75	-0.20486068467755558
2117	76	-0.26489085693677694
1116	1	0.03510992053077189
1116	2	1.3528533428086575E-4
1116	3	0.08099546928085744
1116	4	0.053225131049877916
1116	5	0.05622224161282219
1116	6	0.04888016246842142
1116	7	0.3973007260076784
1116	8	0.43022677299203593
1116	9	0.26640330512744725
1116	10-11	0.13136486708247475
1116	12-13	0.2062186370645307
1116	14-15	0.09219150133489507
1116	16-17	0.24265063313669089
1116	18-19	0.1574725541195079
1116	20-21	0.2314694532660937
1116	22-23	0.14354308441492947
1116	24-25	0.17822777103847898
1116	26-27	0.1351468846517747
1116	28-29	0.24814642842019197
1116	30-31	0.15679444768790773
1116	32-33	0.21421793316873305
1116	34-35	0.08047914219054775
1116	36-37	0.35410104409400134
1116	38-39	0.33483649265980375
1116	40-41	0.33559739848822545
1116	42-43	0.12960980784506404
1116	44-45	0.26352555590372617
1116	46-47	0.12071258451020839
1116	48-49	0.5114999475604023
1116	50-51	0.21359588134193075
1116	52-53	0.4274032513530486
1116	54-55	0.2420751870160629
1116	56-57	0.5504534572578095
1116	58-59	0.23296812687512158
1116	60-61	0.3797222253713315
1116	62-63	0.2942477658575271
1116	64-65	0.6097634612753211
1116	66-67	0.16578750083285598
1116	68-69	0.456268546620187
1116	70-71	-0.021835160283284694
1116	72-73	0.029787649241256986
1116	74-75	0.0044597104778105745
1116	76	0.15719181254608827
2118	1	0.11296868085597112
2118	2	-0.007673348898961763
2118	3	0.1479049064407505
2118	4	0.18133460768402898
2118	5	0.08291229249766019
2118	6	0.2721685266545748
2118	7	0.6375833994581157
2118	8	0.5212611497713624
2118	9	0.18523403807641614
2118	10-11	0.25242549489649235
2118	12-13	0.2844062200724764
2118	14-15	0.2801842958931431
2118	16-17	0.19919419225928436
2118	18-19	0.27815820511278844
2118	20-21	0.3367977440929124
2118	22-23	0.25636215299908116
2118	24-25	0.3112753009704008
2118	26-27	0.20932511819678012
2118	28-29	0.2767077352600964
2118	30-31	0.2803936041913957
2118	32-33	0.27233882937498066
2118	34-35	0.2716558568596952
2118	36-37	0.364213613995652
2118	38-39	0.40037000301681047
2118	40-41	0.31173037424213845
2118	42-43	0.31944651949232394
2118	44-45	0.19490890620521384
2118	46-47	0.30765298091743176
2118	48-49	0.48896022270611184
2118	50-51	0.39463671397730593
2118	52-53	0.49132414675803915
2118	54-55	0.39695583176116855
2118	56-57	0.5225303877435437
2118	58-59	0.4036710458116062
2118	60-61	0.3633327540254925
2118	62-63	0.44396426743763584
2118	64-65	0.3094938683736217
2118	66-67	0.4129212532354032
2118	68-69	-0.02583648433619956
2118	70-71	-0.39024017752015894
2118	72-73	-0.42604404668988494
2118	74-75	-0.4254162718408594
2118	76	-0.2906409706440911
1119	1	0.05287402722584744
1119	2	0.0712918970964651
1119	3	0.12662831761196003
1119	4	-0.04208403960118545
1119	5	0.13200281153240212
1119	6	0.2701873663674945
1119	7	0.6033397183680407
1119	8	0.1665326938737124
1119	9	0.10532453110741358
1119	10-11	0.20140452287652266
1119	12-13	-0.14209289580021078
1119	14-15	0.1914198638149216
1119	16-17	0.22115744246730884
1119	18-19	0.2601894551127941
1119	20-21	0.18463472011746518
1119	22-23	0.25065303088549484
1119	24-25	0.052260777879077125
1119	26-27	0.17007214076802768
1119	28-29	0.10851210258899613
1119	30-31	0.2116146750255936
1119	32-33	0.11160724652705056
1119	34-35	0.15898975794144832
1119	36-37	0.16218613453860797
1119	38-39	0.3410710639695864
1119	40-41	0.2278634224010645
1119	42-43	0.3805601948615802
1119	44-45	0.16477576703316998
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2101	32-33	-0.2822976359145244
2101	34-35	0.01564920837218864
2101	36-37	-0.743644287357462
2101	38-39	-0.20845054861401024
2101	40-41	-0.13112646798783345
2101	42-43	0.035869890936773174
2101	44-45	-0.39389610151608423
2101	46-47	-0.2405464101430539
2101	48-49	-0.2849342839520759
2101	50-51	-0.13639009508816713
2101	52-53	-0.43034588087095926
2101	54-55	-0.031226114510545244
2101	56-57	-1.0782204119247396
2101	58-59	-0.23248962672033002
2101	60-61	-0.4830303270719938
2101	62-63	-0.19534497989386068
2101	64-65	-0.972559653630853
2101	66-67	-0.13717810775741412
2101	68-69	0.1410870391785899
2101	70-71	0.1066946222354197
2101	72-73	0.04907283789808048
2101	74-75	0.03460165617825339
2101	76	-0.0717063601970338
2107	1	-0.1395502151983976
2107	2	-0.08322699610383211
2107	3	0.002939062253496161
2107	4	0.06916064812978817
2107	5	0.1062475454570091
2107	6	-0.027871345753716525
2107	7	0.010991695455992101
2107	8	0.013046228611337085
2107	9	-0.0918299404373002
2107	10-11	0.07395972428080455
2107	12-13	0.1298259881613575
2107	14-15	0.13011858887035288
2107	16-17	0.005781310723044442
2107	18-19	0.14786441353851387
2107	20-21	-0.15532566883301513
2107	22-23	-0.07970081242566351
2107	24-25	-0.10174998523991974
2107	26-27	0.11820014335487627
2107	28-29	-0.156877887248406
2107	30-31	0.05520184243135873
2107	32-33	-0.01651486637020838
2107	34-35	0.1857462712452147
2107	36-37	-0.005350704892286728
2107	38-39	0.28937575724378917
2107	40-41	0.12454787362057829
2107	42-43	0.34014884046394656
2107	44-45	0.1510470161093025
2107	46-47	-0.05054410759339589
2107	48-49	-0.17101829143569347
2107	50-51	0.08519382411986243
2107	52-53	-0.10185330575447438
2107	54-55	0.24264306844683148
2107	56-57	-0.21988622268738567
2107	58-59	0.22407576132641793
2107	60-61	-0.07781162848916523
2107	62-63	0.14103933242586209
2107	64-65	-0.47357781020825485
2107	66-67	0.1633790354161988
2107	68-69	0.1290613526596438
2107	70-71	0.14421101028015215
2107	72-73	0.11117270276594127
2107	74-75	0.0493157204603385
2107	76	0.06243165718016641
2106	1	-0.06263005924574827
2106	2	-0.06593284973436653
2106	3	-0.08044048760839217
2106	4	-0.09719434370222757
2106	5	-0.1535794196016127
2106	6	-0.06809141030716148
2106	7	0.051899725587254864
2106	8	-0.12350609697444526
2106	9	-0.1125201697257765
2106	10-11	0.02240644937963765
2106	12-13	-0.08847360328302756
2106	14-15	-0.20101225737511896
2106	16-17	-0.09144688218861319
2106	18-19	0.07688871655093266
2106	20-21	-0.005583588616460133
2106	22-23	-0.18325731510685017
2106	24-25	-0.06981372323361512
2106	26-27	0.0024108799557680527
2106	28-29	-0.16810337210733195
2106	30-31	-0.2353223961266977
2106	32-33	-0.1745305721283117
2106	34-35	0.035391481445621764
2106	36-37	-0.0535996730031556
2106	38-39	0.09507780962812262
2106	40-41	-0.07625407441982901
2106	42-43	0.28286146368782994
2106	44-45	0.018272448844733447
2106	46-47	-0.32193081560562575
2106	48-49	-0.1882698128441902
2106	50-51	-0.07243864468492234
2106	52-53	-0.11303746873991827
2106	54-55	0.14883644943008534
2106	56-57	-0.0580240503586964
2106	58-59	0.15939518307003198
2106	60-61	0.045589787396160375
2106	62-63	0.10389609435684122
2106	64-65	-0.19704082659302458
2106	66-67	0.1258059292465532
2106	68-69	0.055621528001378095
2106	70-71	0.029702768036404592
2106	72-73	0.046501063043567825
2106	74-75	-0.05504325795784126
2106	76	-0.10869989337871289
2105	1	0.05431497805464147
2105	2	-0.2282373715961441
2105	3	-0.12294020456874932
2105	4	-0.02163746492349361
2105	5	-0.05496010303080823
2105	6	-0.16663987936264846
2105	7	-0.06421574167621458
2105	8	-0.05732077716151451
2105	9	-0.05838404569691846
2105	10-11	-0.09186778950174102
2105	12-13	-0.1401445480904897
2105	14-15	0.014042667607107262
2105	16-17	0.002911495762617733
2105	18-19	-0.14626061138626767
2105	20-21	0.11493937563083989
2105	22-23	-0.011361269309283273
2105	24-25	0.03769411071552753
2105	26-27	0.08097030446666764
2105	28-29	-0.10230425195447168
2105	30-31	-0.268408260108437
2105	32-33	-0.06262632704756044
2105	34-35	-0.026408130817145548
2105	36-37	0.01416995738115645
2105	38-39	0.05649809885716195
2105	40-41	-0.08597204503012534
2105	42-43	0.1967285119966391
2105	44-45	-0.00936164998793032
2105	46-47	-0.2290070998394924
2105	48-49	-0.03211745050536052
2105	50-51	-0.10730776993451485
2105	52-53	-0.002486079900315019
2105	54-55	0.05848281435471847
2105	56-57	0.19702445629737753
2105	58-59	0.08771099385475623
2105	60-61	0.22938131365530268
2105	62-63	-0.07916083491349468
2105	64-65	-0.09189691258678323
2105	66-67	0.019407986610829653
2105	68-69	0.03930427911403456
2105	70-71	0.16907719212160188
2105	72-73	0.043172589008790396
2105	74-75	0.061660407209664925
2105	76	0.10129420202456174
2104	1	-0.035322916392786397
2104	2	-0.10155574346578078
2104	3	-0.09138635471370549
2104	4	-0.2811542001133631
2104	5	-0.0867662582532347
2104	6	-0.08749946863485292
2104	7	0.12353168754344068
2104	8	0.14860913274608833
2104	9	-0.14290214181805538
2104	10-11	-0.1961911357319437
2104	12-13	-0.25707196793289455
2104	14-15	-0.26054399812635864
2104	16-17	-0.15038395852626962
2104	18-19	-0.28391150228824813
2104	20-21	-0.041081171044588416
2104	22-23	-0.07036187288543516
2104	24-25	-0.025753633663192943
2104	26-27	-0.08763408999919875
2104	28-29	-0.25034707600851647
2104	30-31	-0.09793496835311544
2104	32-33	-0.28809036697891344
2104	34-35	-0.13394456050115622
2104	36-37	-0.1270327132551614
2104	38-39	-0.13474877382314787
2104	40-41	-0.40814774890754535
2104	42-43	-0.1743644550367165
2104	44-45	-0.23917640529248985
2104	46-47	-0.3875438328167462
2104	48-49	-0.1648466934186743
2104	50-51	-0.2363198517429197
2104	52-53	-0.22403471431554323
2104	54-55	-0.15542519446862713
2104	56-57	-0.03254862395542801
2104	58-59	-0.31547219928384607
2104	60-61	-0.11819457300082092
2104	62-63	-0.357345909523886
2104	64-65	-0.26558435396326274
2104	66-67	-0.19783900748711858
2104	68-69	0.023839992082717032
2104	70-71	0.04314771369819326
2104	72-73	0.15064592667201637
2104	74-75	0.09826861153047872
2104	76	0.15930969832797004
2111	1	0.048140538938781674
2111	2	-0.17239930155334804
2111	3	0.009294161086927488
2111	4	-0.1863338714807341
2111	5	-0.14310010103191217
2111	6	-0.015379289308775412
2111	7	-0.0654405596346237
2111	8	-0.22251862530050204
2111	9	-0.031319850078766365
2111	10-11	-0.23811353456517992
2111	12-13	-0.25904808762074794
2111	14-15	-0.3784176143131006
2111	16-17	-0.19162573030826735
2111	18-19	-0.43929008560827043
2111	20-21	-0.1668086469596588
2111	22-23	-0.04443213925892309
2111	24-25	-0.08471702858980734
2111	26-27	-0.15786094892667535
2111	28-29	-0.2696064296705458
2111	30-31	-0.052996256720767576
2111	32-33	-0.169699167676022
2111	34-35	-0.14525295738329902
2111	36-37	0.03155718044364875
2111	38-39	-0.18182938913949442
2111	40-41	-0.23265132812777978
2111	42-43	-0.2055140050156723
2111	44-45	-0.13964498457947627
2111	46-47	-0.2556719656542512
2111	48-49	-0.20146820717509684
2111	50-51	-0.22573728261879467
2111	52-53	-0.18922353625080035
2111	54-55	-0.31150449175380146
2111	56-57	-0.1589930687844472
2111	58-59	-0.2409784884038828
2111	60-61	-0.013579453104817674
2111	62-63	-0.27495297339309843
2111	64-65	-0.014826448948852544
2111	66-67	-0.1615301697229654
2111	68-69	0.05686483574266177
2111	70-71	-0.02861699935848705
2111	72-73	-0.0011986371209715685
2111	74-75	-0.02568250132987515
2111	76	-0.08465359688513985
2110	1	0.0527840945468796
2110	2	-0.06871039939873214
2110	3	-0.002657215431874249
2110	4	-0.048176095333751334
2110	5	0.009536825356335044
2110	6	0.006671367986285759
2110	7	0.10661503441716746
2110	8	0.21073902192243565
2110	9	0.29306542221178944
2110	10-11	0.14299525531395574
2110	12-13	0.23710278486169756
2110	14-15	0.24985956395367026
2110	16-17	0.05173619940497787
2110	18-19	-0.08111963547511891
2110	20-21	0.03699581427698462
2110	22-23	0.1456588765096285
2110	24-25	-0.041563015095896105
2110	26-27	0.0986380517860681
2110	28-29	0.07869046639237354
2110	30-31	0.17938577387445065
2110	32-33	0.19876787110951
2110	34-35	0.1532914788675015
2110	36-37	0.21777091375388125
2110	38-39	0.25615887680683613
2110	40-41	0.07385009630517203
2110	42-43	-0.08866957992129443
2110	44-45	0.1299371319542928
2110	46-47	0.23197903779839635
2110	48-49	0.19655429290569515
2110	50-51	0.3092385427005553
2110	52-53	0.22776059581133978
2110	54-55	0.1765830452251862
2110	56-57	0.20864481004058888
2110	58-59	0.16047434713765085
2110	60-61	0.018374557433538996
2110	62-63	0.22568912976920075
2110	64-65	0.2898458380962694
2110	66-67	0.3130380223961211
2110	68-69	0.021768595781207267
2110	70-71	0.14994670973078073
2110	72-73	0.14954011442196702
2110	74-75	0.27138273482491826
2110	76	0.09926357018299115
2109	1	-0.0018924317913970867
2109	2	-0.13369681842812753
2109	3	0.0013042827294427184
2109	4	0.18232851338819245
2109	5	0.05944024206461762
2109	6	0.22748144286606475
2109	7	-0.13702081940950706
2109	8	0.25510714233185183
2109	9	0.2706061502559649
2109	10-11	0.1318093180919675
2109	12-13	0.2503772815203362
2109	14-15	-0.01325313680544582
2109	16-17	0.13316399909486165
2109	18-19	0.12634202655151228
2109	20-21	-0.04697382651964688
2109	22-23	0.016823029851558147
2109	24-25	-0.0571981085039468
2109	26-27	0.003238845668903423
2109	28-29	0.09394044818957781
2109	30-31	0.03158557125689043
2109	32-33	0.15433427288603951
2109	34-35	0.13819364910644083
2109	36-37	0.3440452498421962
2109	38-39	0.011015586677473266
2109	40-41	0.12838939850414732
2109	42-43	-0.06012623565771236
2109	44-45	0.14452930181229817
2109	46-47	0.14800208373870305
2109	48-49	0.07340261968736428
2109	50-51	-0.10239750978969653
2109	52-53	0.04119081675384706
2109	54-55	0.004763776207532544
2109	56-57	-0.06348428014287322
2109	58-59	-0.13768947734853754
2109	60-61	-0.3309680846924934
2109	62-63	0.06213921240907183
2109	64-65	-0.07947609624413587
2109	66-67	-0.04096513725498596
2109	68-69	-0.2829552256703778
2109	70-71	-0.11956471447014039
2109	72-73	-0.14627197361429012
2109	74-75	-0.10678179848522973
2109	76	-0.1283602960000465
2108	1	0.03411374303129833
2108	2	0.04370863099572375
2108	3	0.03713404088581029
2108	4	0.02891223975262136
2108	5	0.02801433780253859
2108	6	-0.2126658319372865
2108	7	-0.11434060581528627
2108	8	0.24693486567237954
2108	9	0.19427245003288363
2108	10-11	-0.10812631455986121
2108	12-13	0.2591333150241866
2108	14-15	-0.04567662106332904
2108	16-17	0.2262388610005459
2108	18-19	0.12285969241460037
2108	20-21	0.10254555176599922
2108	22-23	-0.01312930453550365
2108	24-25	0.03581489586191111
2108	26-27	-0.19774345246257496
2108	28-29	0.04417321486050696
2108	30-31	-0.14843045186064074
2108	32-33	0.10590953368409117
2108	34-35	8.66778905262322E-4
2108	36-37	0.300237664332478
2108	38-39	0.03141739418630607
2108	40-41	0.10230626220998573
2108	42-43	-0.02891348158649265
2108	44-45	0.22395181968825284
2108	46-47	-0.006727929543608013
2108	48-49	0.12045766619541354
2108	50-51	-0.004977858864108953
2108	52-53	-0.07313097307022787
2108	54-55	-0.05006206140560465
2108	56-57	-0.017570865299084915
2108	58-59	-0.20689788116509078
2108	60-61	-0.3200147221657019
2108	62-63	0.0046080499529068675
2108	64-65	-0.18326167794377568
2108	66-67	-0.23198703498366768
2108	68-69	-0.14695331564678682
2108	70-71	0.23417506751604122
2108	72-73	0.22595092505356362
2108	74-75	0.22243565795587017
2108	76	0.1585131710011609
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	15.0
12	95.0
13	463.0
14	1004.0
15	2814.0
16	3174.0
17	3370.0
18	3726.0
19	3533.0
20	3510.0
21	3555.0
22	4015.0
23	4874.0
24	6308.0
25	8378.0
26	11198.0
27	15501.0
28	20760.0
29	27624.0
30	36964.0
31	50994.0
32	74744.0
33	124432.0
34	428933.0
35	537502.0
36	124397.0
37	1145126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	61.998107294848324	7.558089236703369	7.608372481351565	22.83543098709674
2	16.02857563817288	56.351468260414585	14.758954442937503	12.861001658475036
3	15.095296202129951	13.996924832169128	56.84742407471071	14.060354890990212
4	16.247690790741252	16.41720280618509	10.026293818308204	57.308812584765455
5	61.14215662199992	17.18070577746212	11.462895871190513	10.214241729347453
6	57.01436715388306	19.381906377384293	12.45110520927386	11.152621259458785
7	10.64937419956857	55.900091046123734	21.17989731810609	12.2706374362016
8	12.280195390270531	57.25127596797896	15.814749472045817	14.653779169704686
9	56.95384603760469	12.608868119123088	17.287883309847714	13.149402533424507
10-11	35.979709181302674	39.64618569631396	12.215386417127249	12.158718705256119
12-13	35.107933101877215	14.119497092946382	15.635433942448271	35.13713586272814
14-15	12.844492465083245	14.22414346753507	37.37481157985803	35.55655248752366
16-17	36.06820525800809	14.233323636858191	36.55819207332046	13.140279031813254
18-19	35.539854401758966	14.540254853589522	37.10471437584293	12.815176368808581
20-21	35.54610673930208	36.91655868319349	14.468097967140285	13.069236610364147
22-23	58.46685505532657	15.046354188310584	14.177694833038032	12.309095923324808
24-25	13.385725781164409	59.72529382208606	14.464660125953435	12.424320270796105
26-27	35.80475706551921	37.13163153898172	14.031397690224065	13.032213705275009
28-29	35.74495374025787	36.888187048783344	14.265586454150153	13.101272756808626
30-31	35.265884904099345	14.523651214011279	36.882671391494554	13.327792490394824
32-33	12.850197014744937	36.95828500836793	37.10818999550436	13.083327981382768
34-35	35.37718029021424	37.027684066172775	14.359560409669777	13.235575233943203
36-37	13.028568082477964	14.620005213429494	36.884088084291335	35.46733861980121
38-39	13.170369586816824	14.5697409529998	36.56818447984707	35.691704980336304
40-41	57.72469692218768	14.30940948466383	14.34354233644754	13.622351256700957
42-43	35.307535672324626	14.340671172379402	37.0305930087155	13.32120014658048
44-45	35.19782698214212	14.509408729094336	37.00962595532318	13.283138333440373
46-47	35.409310882845176	14.497754069686176	14.391691002300707	35.70124404516795
48-49	13.262586843268442	14.525804587062382	14.637723318007865	57.5738852516613
50-51	35.579502910831465	14.797941828704841	36.59495052908754	13.027604731376158
52-53	13.035370670184598	36.86613138335922	37.078616453775474	13.019881492680701
54-55	12.677077154978637	37.03276527100389	14.848659430829503	35.44149814318797
56-57	12.609604799377411	15.033906180936226	59.26371641965841	13.092772600027955
58-59	12.981439435438476	59.49136950748203	14.461033392393682	13.066157664685814
60-61	13.252745550640158	59.840518169557356	14.152156584221443	12.754579695581054
62-63	12.937106395517961	59.46653016044519	14.775501414803873	12.820862029232986
64-65	12.967008058148627	59.533530285114146	14.454044374596242	13.045417282140981
66-67	12.789675898466571	59.446262008832605	14.79246394989063	12.971598142810189
68-69	15.0441630367849	48.57352257830534	19.01407625962879	17.36823812528098
70-71	17.229156671111934	41.94999263319746	21.381237698384215	19.439612997306394
72-73	16.967521845402928	40.360368869025805	22.74451173210528	19.927597553465986
74-75	17.368200346806397	38.73547889883302	23.186500995462804	20.709819758897773
76	16.211687904465794	40.194256916294236	23.38631134752041	20.20774383171956
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	398.0
1	238.5
2	77.5
3	82.5
4	89.0
5	91.0
6	83.5
7	73.5
8	73.0
9	81.0
10	93.5
11	105.5
12	113.0
13	122.5
14	148.5
15	189.0
16	281.0
17	349.0
18	464.0
19	674.5
20	967.5
21	1165.0
22	1421.5
23	2025.5
24	3013.0
25	3653.0
26	4328.0
27	5857.0
28	7844.0
29	8977.0
30	10348.0
31	13694.0
32	17777.5
33	19886.0
34	22797.5
35	28995.0
36	37191.5
37	42102.0
38	48213.5
39	150856.0
40	314727.0
41	379989.5
42	377912.0
43	337944.5
44	254255.0
45	177077.5
46	143622.0
47	125661.0
48	98445.0
49	81482.0
50	73774.0
51	68072.5
52	56967.5
53	51727.0
54	51890.0
55	48781.0
56	42442.5
57	31393.5
58	23574.0
59	25084.0
60	26267.5
61	26525.0
62	27109.0
63	27178.5
64	27158.0
65	24496.0
66	18197.0
67	14470.0
68	10118.0
69	5958.5
70	8194.5
71	10238.0
72	8789.5
73	6425.5
74	3408.5
75	1307.0
76	920.0
77	348.0
78	120.5
79	78.0
80	64.0
81	38.0
82	24.5
83	23.0
84	23.0
85	20.5
86	16.5
87	15.0
88	12.5
89	10.5
90	9.5
91	8.0
92	8.0
93	11.5
94	13.5
95	15.0
96	18.0
97	15.5
98	23.0
99	116.0
100	199.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.8889237290376689E-4
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	1.888923729037669E-5
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	2647010.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.63974684818461
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.94138294897661	26.219283914009857
2	14.70396999190188	9.892756563817647
3	3.8752462192818555	3.9108690537307838
4	1.2764830996082819	1.7176227330723448
5	0.5454291931515248	0.9174049990613442
6	0.35417988391813193	0.7148712980235222
7	0.25653776956549185	0.6040905937626745
8	0.17564333435731908	0.47268778426810076
9	0.12349886066697548	0.3739023367988651
>10	0.6265953140736946	3.950905479144896
>50	0.05634010796153841	1.3128322052440573
>100	0.053686698600051666	3.838508674679459
>500	0.007062963894555209	1.5813725075410896
>1k	0.0034931536266692297	1.922934031971068
>5k	3.378453116420502E-4	0.7201641156659413
>10k+	1.126151038806834E-4	41.849793709208285
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	1104699	41.733843090883674	Illumina Single End PCR Primer 1 (100% over 50bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG	6620	0.2500935017245874	No Hit
CATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	6214	0.2347554410448015	Illumina Single End PCR Primer 1 (98% over 50bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC	6176	0.23331985901073288	No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG	3985	0.15054722120430222	No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG	3359	0.12689789611675062	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.02720050169814243	0.0	0.0
2	0.028711640681372566	0.0	0.0
3	0.031696140173252084	0.0	0.0
4	0.03396284864809729	0.0	0.0
5	0.03789181000449564	0.0	0.0
6	0.040536303225148375	0.0	0.0
7	0.04348302424244714	0.0	0.0
8	0.05043426356530576	0.0	0.0
9	0.05538324373538445	7.555694916150676E-5	0.0
10	0.07015462729645902	7.555694916150676E-5	0.0
11	0.07850367017880552	7.555694916150676E-5	0.0
12	0.08360376424720722	7.555694916150676E-5	0.0
13	0.09671289492672865	7.555694916150676E-5	0.0
14	0.10959535475876556	7.555694916150676E-5	0.0
15	0.11329764526767938	7.555694916150676E-5	0.0
16	0.1330557874734134	7.555694916150676E-5	0.0
17	0.1355491667957431	7.555694916150676E-5	0.0
18	0.13740031205020004	1.1333542374226014E-4	0.0
19	0.150887227475529	1.1333542374226014E-4	0.0
20	0.15254948035708213	1.1333542374226014E-4	0.0
21	0.15576065069644618	1.1333542374226014E-4	0.0
22	0.15780068832380686	1.1333542374226014E-4	0.0
23	0.16033184612071733	1.1333542374226014E-4	0.0
24	0.1619940990022705	1.1333542374226014E-4	0.0
25	0.16463859222292324	1.1333542374226014E-4	0.0
26	0.16777420561312575	1.1333542374226014E-4	0.0
27	0.17196761629158938	1.1333542374226014E-4	0.0
28	0.17634991934295677	1.1333542374226014E-4	0.0
29	0.18575675951356435	1.1333542374226014E-4	0.0
30	0.22406413273844827	1.1333542374226014E-4	0.0
31	0.22765308782361987	1.1333542374226014E-4	0.0
32	0.23203539087498726	1.1333542374226014E-4	0.0
33	0.234830997993963	1.1333542374226014E-4	0.0
34	0.23736215579087347	1.1333542374226014E-4	0.0
35	0.23947775036739566	1.1333542374226014E-4	0.0
36	0.24208446511346765	1.1333542374226014E-4	0.0
37	0.2447289583341204	1.1333542374226014E-4	0.0
38	0.24794012867348442	1.1333542374226014E-4	0.0
39	0.25020683714832964	1.1333542374226014E-4	0.0
40	0.25326689358937066	1.5111389832301353E-4	0.0
41	0.2567802917253807	1.5111389832301353E-4	0.0
42	0.2603692468105523	1.5111389832301353E-4	0.0
43	0.2641093157940469	1.5111389832301353E-4	0.0
44	0.26762271393005693	1.5111389832301353E-4	0.0
45	0.2711738905406478	1.5111389832301353E-4	0.0
46	0.2750272949478846	1.5111389832301353E-4	0.0
47	0.2802785029146093	1.5111389832301353E-4	0.0
48	0.28537859698301105	1.5111389832301353E-4	0.0
49	0.2901009063056052	1.5111389832301353E-4	0.0
50	0.2956921205435567	1.5111389832301353E-4	0.0
51	0.301774454951058	1.5111389832301353E-4	0.0
52	0.30793234630772076	1.5111389832301353E-4	0.0
53	0.31265465563031497	1.5111389832301353E-4	0.0
54	0.3198325658006581	1.5111389832301353E-4	0.0
55	0.32723714681848576	1.5111389832301353E-4	0.0
56	0.3339239368192791	1.5111389832301353E-4	0.0
57	0.34378411868485576	1.5111389832301353E-4	0.0
58	0.3533420727537864	1.5111389832301353E-4	0.0
59	0.3635422608905898	1.5111389832301353E-4	0.0
60	0.37491358173939654	1.5111389832301353E-4	0.0
61	0.3867760227577531	1.5111389832301353E-4	0.0
62	0.3981851220811406	1.5111389832301353E-4	0.0
63	0.41080313259111223	1.5111389832301353E-4	0.0
64	0.4253100668301215	1.5111389832301353E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTAT	116955	0.0	68.94361	47
CGTATCA	117075	0.0	68.860985	49
CCGTATC	116870	0.0	68.85899	48
GGTCGCC	117510	0.0	68.85627	43
GAGCGTC	119080	0.0	68.85077	9
GTATCAT	117235	0.0	68.8297	50
TGTAGGG	119210	0.0	68.828545	17
CGCCGTA	117105	0.0	68.819435	46
TCGGAAG	119865	0.0	68.808655	3
TCGTGTA	118850	0.0	68.792595	14
TGGTCGC	117825	0.0	68.78803	42
GTGTAGG	119350	0.0	68.78593	16
AGCGTCG	119265	0.0	68.77919	10
CGTGTAG	119110	0.0	68.76878	15
GTCGTGT	119145	0.0	68.7574	13
GTAGGGA	119320	0.0	68.738686	18
TCATTAA	117535	0.0	68.7106	53
CATTAAA	117725	0.0	68.70376	54
GTGGTCG	118470	0.0	68.69419	41
CGTCGTG	119295	0.0	68.66507	12
>>END_MODULE