FastQCFastQC Report
Mon 15 Sep 2014
TGA3_5_GCCAAT_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameTGA3_5_GCCAAT_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2647010
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG110469941.733843090883674Illumina Single End PCR Primer 1 (100% over 50bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG66200.2500935017245874No Hit
CATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG62140.2347554410448015Illumina Single End PCR Primer 1 (98% over 50bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC61760.23331985901073288No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG39850.15054722120430222No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG33590.12689789611675062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT1169550.068.9436147
CGTATCA1170750.068.86098549
CCGTATC1168700.068.8589948
GGTCGCC1175100.068.8562743
GAGCGTC1190800.068.850779
GTATCAT1172350.068.829750
TGTAGGG1192100.068.82854517
CGCCGTA1171050.068.81943546
TCGGAAG1198650.068.8086553
TCGTGTA1188500.068.79259514
TGGTCGC1178250.068.7880342
GTGTAGG1193500.068.7859316
AGCGTCG1192650.068.7791910
CGTGTAG1191100.068.7687815
GTCGTGT1191450.068.757413
GTAGGGA1193200.068.73868618
TCATTAA1175350.068.710653
CATTAAA1177250.068.7037654
GTGGTCG1184700.068.6941941
CGTCGTG1192950.068.6650712
Produced by FastQC (version 0.11.2)