##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	TGA3_5_GCCAAT_L001_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2647010
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.76509306727213	34.0	34.0	34.0	34.0	34.0
2	33.78197928984024	34.0	34.0	34.0	34.0	34.0
3	33.70702075171609	34.0	34.0	34.0	34.0	34.0
4	33.76984182152693	34.0	34.0	34.0	34.0	34.0
5	33.83439314547357	34.0	34.0	34.0	34.0	34.0
6	37.52296402355866	38.0	38.0	38.0	37.0	38.0
7	37.46497784292466	38.0	38.0	38.0	37.0	38.0
8	37.43862395684187	38.0	38.0	38.0	37.0	38.0
9	37.494591255794276	38.0	38.0	38.0	37.0	38.0
10-11	37.51086433371994	38.0	38.0	38.0	37.0	38.0
12-13	37.44665868281571	38.0	38.0	38.0	37.0	38.0
14-15	37.452363421369775	38.0	38.0	38.0	37.0	38.0
16-17	37.466696385733336	38.0	38.0	38.0	37.0	38.0
18-19	37.34081303055145	38.0	38.0	38.0	36.5	38.0
20-21	37.42970842573318	38.0	38.0	38.0	37.0	38.0
22-23	37.23848795433338	38.0	38.0	38.0	37.0	38.0
24-25	37.435496465823704	38.0	38.0	38.0	37.0	38.0
26-27	37.377557697175305	38.0	38.0	38.0	37.0	38.0
28-29	37.45066376779838	38.0	38.0	38.0	37.0	38.0
30-31	37.36007986369526	38.0	38.0	38.0	37.0	38.0
32-33	37.298991314728696	38.0	38.0	38.0	37.0	38.0
34-35	36.773881851598595	38.0	38.0	38.0	35.0	38.0
36-37	36.67869048473561	38.0	38.0	38.0	35.0	38.0
38-39	36.58171030710122	38.0	38.0	38.0	34.0	38.0
40-41	36.23385706891927	38.0	37.0	38.0	33.5	38.0
42-43	36.66392665686945	38.0	37.0	38.0	35.0	38.0
44-45	37.13582438298306	38.0	38.0	38.0	36.5	38.0
46-47	37.20790136795856	38.0	38.0	38.0	36.5	38.0
48-49	37.24969720552623	38.0	38.0	38.0	37.0	38.0
50-51	37.0994046112406	38.0	38.0	38.0	36.5	38.0
52-53	37.115141801504336	38.0	38.0	38.0	36.0	38.0
54-55	37.076759626899786	38.0	38.0	38.0	36.0	38.0
56-57	37.061892286013276	38.0	38.0	38.0	36.5	38.0
58-59	37.15604757821089	38.0	38.0	38.0	36.5	38.0
60-61	36.98666363179588	38.0	38.0	38.0	36.0	38.0
62-63	37.03282477210135	38.0	38.0	38.0	36.0	38.0
64-65	37.00986225968168	38.0	38.0	38.0	36.5	38.0
66-67	37.125972701274264	38.0	38.0	38.0	36.5	38.0
68-69	37.05175443235953	38.0	38.0	38.0	36.0	38.0
70-71	36.94392843245775	38.0	38.0	38.0	36.0	38.0
72-73	36.55991552733084	38.0	38.0	38.0	34.0	38.0
74-75	30.582075624950416	37.0	26.0	38.0	11.0	38.0
76	28.811674304214943	35.0	22.0	38.0	11.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.023294468288462156
1101	2	0.06318918154055098
1101	3	-0.020422538074406305
1101	4	0.08137726041118754
1101	5	0.035764392540031054
1101	6	0.11977139348606869
1101	7	-6.778968483445169E-5
1101	8	0.035158986309362206
1101	9	0.11657746437575156
1101	10-11	0.0472976940460299
1101	12-13	0.08175274467476612
1101	14-15	0.0433726234923455
1101	16-17	0.13899843457306105
1101	18-19	0.038666327641735165
1101	20-21	0.012384410788769173
1101	22-23	0.3147186846554746
1101	24-25	0.0824835972726703
1101	26-27	0.06518587266535292
1101	28-29	0.04228252174100078
1101	30-31	0.0738755860279312
1101	32-33	0.0803187322646366
1101	34-35	0.027914805596452652
1101	36-37	-0.02263977916627624
1101	38-39	0.048864021634777544
1101	40-41	0.05627480880896485
1101	42-43	0.09522064150537801
1101	44-45	0.15255748178240225
1101	46-47	0.08718531542385932
1101	48-49	0.07437884811047724
1101	50-51	0.12040616937794368
1101	52-53	-0.03172962303695215
1101	54-55	0.08911138479091818
1101	56-57	0.11173197563189063
1101	58-59	0.08371967102743127
1101	60-61	0.1526126653222164
1101	62-63	0.06524939219772818
1101	64-65	0.07855684112058725
1101	66-67	0.07117417073970955
1101	68-69	0.21300362052127042
1101	70-71	0.04432721352002034
1101	72-73	0.3179200440364838
1101	74-75	-0.026312034471992263
1101	76	0.11729190587731608
1102	1	0.04840277365706669
1102	2	0.06140994527107324
1102	3	0.053039402090355736
1102	4	0.047867486662802605
1102	5	0.020927638549260053
1102	6	0.05094541750174386
1102	7	0.03804086794660577
1102	8	-0.11838990715276765
1102	9	0.03248398494604743
1102	10-11	0.07342478058526325
1102	12-13	0.0749783288439474
1102	14-15	-0.012314258116440158
1102	16-17	0.04298734756414291
1102	18-19	0.07678224683139945
1102	20-21	-0.13185933654608561
1102	22-23	0.2731216980476461
1102	24-25	-0.10662128434847773
1102	26-27	-0.07874361914917927
1102	28-29	-0.1359944495403127
1102	30-31	-0.050363186367022195
1102	32-33	0.1649285548354129
1102	34-35	0.19405029700308773
1102	36-37	0.1739063456336467
1102	38-39	0.13256259589734043
1102	40-41	0.17814893973949353
1102	42-43	0.20392755883486302
1102	44-45	0.15838913105222474
1102	46-47	0.12117682403575714
1102	48-49	0.04626642064881281
1102	50-51	0.13256294487528208
1102	52-53	0.03569533571122463
1102	54-55	0.1908541587679693
1102	56-57	0.17041494526930023
1102	58-59	0.16049025522751492
1102	60-61	0.07340049804063398
1102	62-63	0.07081359982267799
1102	64-65	0.08696016351522928
1102	66-67	0.1026422106133964
1102	68-69	0.16043967245840918
1102	70-71	0.11489905350617136
1102	72-73	0.12871025607398678
1102	74-75	-0.2825545025365841
1102	76	0.004331723936658705
1103	1	0.012629622650628392
1103	2	0.02255423544640678
1103	3	-0.0401266289897535
1103	4	-0.003909061539900449
1103	5	0.03346311595669249
1103	6	0.024812273477188285
1103	7	0.010527957305569657
1103	8	-0.026052451501449525
1103	9	0.05395792898112717
1103	10-11	0.009942584512934616
1103	12-13	0.05568592798383776
1103	14-15	0.11097426857480741
1103	16-17	0.14397742550598736
1103	18-19	0.14850290083485618
1103	20-21	0.020956025096808162
1103	22-23	0.3050019405059743
1103	24-25	-0.05843839578182042
1103	26-27	0.04083315606781923
1103	28-29	0.015570082283325348
1103	30-31	0.07172623736166628
1103	32-33	0.06549403464210002
1103	34-35	0.13466626189297415
1103	36-37	0.10671068139686923
1103	38-39	0.07099413932929366
1103	40-41	-0.008638651818174026
1103	42-43	0.12732265395285935
1103	44-45	0.08360049467784592
1103	46-47	0.026460241363551518
1103	48-49	0.007553854003710114
1103	50-51	0.0745467417869321
1103	52-53	-0.10590256569155088
1103	54-55	0.04670684906822231
1103	56-57	-0.12326077832589277
1103	58-59	-0.0752153794842485
1103	60-61	-0.12062679753908867
1103	62-63	0.05752545142004806
1103	64-65	-0.24213046765315482
1103	66-67	-0.016558773923861736
1103	68-69	-0.21685175430851444
1103	70-71	-0.04904790302826001
1103	72-73	-0.422523787187842
1103	74-75	0.39193584092686606
1103	76	-0.09937723149128175
2116	1	-0.048607190741911666
2116	2	-0.05424171905866615
2116	3	0.07600170141913765
2116	4	0.0078957547514662
2116	5	0.0071186158744538375
2116	6	-0.07583501697182271
2116	7	0.02792295810789369
2116	8	-0.07055997386504487
2116	9	-0.005237958924816155
2116	10-11	-0.004003498344268053
2116	12-13	0.03850461949594575
2116	14-15	0.12647525609993693
2116	16-17	0.11830850843759322
2116	18-19	0.1801345312386502
2116	20-21	-0.010977513315729936
2116	22-23	0.36174455940411576
2116	24-25	0.01616449283474708
2116	26-27	0.08159473882668777
2116	28-29	-0.012332197972732217
2116	30-31	0.05628692239966426
2116	32-33	-0.00487874352689488
2116	34-35	4.4953159651583974E-4
2116	36-37	-0.08645512590241111
2116	38-39	-0.1365758410328084
2116	40-41	-0.08561308877200702
2116	42-43	-0.020365559971907032
2116	44-45	-0.0750154861371044
2116	46-47	-0.11674485781324506
2116	48-49	-0.02395768883992133
2116	50-51	-0.06594219073279817
2116	52-53	-0.17690666821399503
2116	54-55	0.021060513769754152
2116	56-57	-0.02613377064970024
2116	58-59	-0.14616977396102726
2116	60-61	-0.21386863482245388
2116	62-63	-0.02651167035540425
2116	64-65	-0.1728346167065169
2116	66-67	0.034210373627402646
2116	68-69	-0.1310369465390977
2116	70-71	-0.11285634366353747
2116	72-73	0.12472853624102243
2116	74-75	-0.09546966452071004
2116	76	-0.39396830439279285
1117	1	0.0019240460288898475
1117	2	0.06142556175025504
1117	3	0.09161299744456386
1117	4	0.029887713632675172
1117	5	-0.011286503406608972
1117	6	-0.11427118752338572
1117	7	-0.16388263432571648
1117	8	-0.07053052100068413
1117	9	0.02858390925901233
1117	10-11	-0.0023168958883630353
1117	12-13	0.09138825167510589
1117	14-15	0.08739478385611221
1117	16-17	-0.01746777636754615
1117	18-19	0.24125640544691862
1117	20-21	0.021181008410117386
1117	22-23	0.4505060705145638
1117	24-25	0.04983244343264914
1117	26-27	0.14773856982080247
1117	28-29	0.09823383455807999
1117	30-31	-0.004850824306103618
1117	32-33	-0.05632039227065633
1117	34-35	0.16297843931919687
1117	36-37	0.2178899829646639
1117	38-39	0.21395311374953963
1117	40-41	0.38092253428488476
1117	42-43	0.3716300460339568
1117	44-45	0.16863010908763698
1117	46-47	0.06401008887848292
1117	48-49	0.06186844473531039
1117	50-51	0.2355393877378944
1117	52-53	0.20800042240082917
1117	54-55	0.3552281894133884
1117	56-57	0.2593281575676656
1117	58-59	0.32150531185695286
1117	60-61	0.24713459627036372
1117	62-63	0.3747955946236132
1117	64-65	0.3024314073657237
1117	66-67	0.38714634955069727
1117	68-69	0.2924665203591914
1117	70-71	0.40031896518078014
1117	72-73	0.5215959543343729
1117	74-75	0.028015769597676154
1117	76	-0.3181285579957489
2117	1	0.0393298715333259
2117	2	0.04618205120760166
2117	3	-0.017487390914716627
2117	4	0.03007813277032767
2117	5	-0.01688675731494982
2117	6	-0.04073867394046715
2117	7	0.011552642389183632
2117	8	-0.019500010242857968
2117	9	0.0749136571463751
2117	10-11	0.002535741698501681
2117	12-13	0.14479014330520812
2117	14-15	0.12029279081257016
2117	16-17	0.02069460461266459
2117	18-19	0.1684697809590574
2117	20-21	0.04203271204222858
2117	22-23	0.3579948131900892
2117	24-25	0.09517775306444776
2117	26-27	0.09807316388127418
2117	28-29	0.10744533509017629
2117	30-31	0.1958609465116723
2117	32-33	0.21480571309617602
2117	34-35	0.17417574520152357
2117	36-37	0.1805401458582807
2117	38-39	0.12678140015083272
2117	40-41	0.36967909832608825
2117	42-43	0.33217694207979775
2117	44-45	0.1396237452784419
2117	46-47	-0.13796786011669582
2117	48-49	0.06555251768955372
2117	50-51	0.25622065149472206
2117	52-53	0.18351834134469414
2117	54-55	0.22732394151312718
2117	56-57	-0.03487502611667992
2117	58-59	0.18430242434648392
2117	60-61	0.09942263695258191
2117	62-63	0.2220311747930097
2117	64-65	0.19387541855354584
2117	66-67	0.1701672976557873
2117	68-69	0.12653657468318613
2117	70-71	0.25803694110494035
2117	72-73	0.3342878342914446
2117	74-75	0.053125601501779585
2117	76	-0.22791774405130738
1116	1	-0.027856228367454605
1116	2	0.037953905697868606
1116	3	0.003840587310627086
1116	4	0.0590186142351925
1116	5	0.05649067449969891
1116	6	-0.0028967259806762513
1116	7	-0.03041958473578177
1116	8	0.08543470480002924
1116	9	0.03281415433689716
1116	10-11	0.10335625312731622
1116	12-13	0.13335794014518143
1116	14-15	0.04401341500780376
1116	16-17	-0.12905533450257423
1116	18-19	0.017426811886707583
1116	20-21	-0.024746123787096508
1116	22-23	0.33764171597496073
1116	24-25	0.045096573041277566
1116	26-27	-0.013441383948901375
1116	28-29	-0.010121652697613115
1116	30-31	0.11897374979123754
1116	32-33	0.13265105051091552
1116	34-35	0.11120086592266887
1116	36-37	0.04553662081304566
1116	38-39	0.042077902064605155
1116	40-41	0.04609013259241834
1116	42-43	0.09951811820174328
1116	44-45	0.05174896474192536
1116	46-47	0.08351255701335702
1116	48-49	0.10460181301974103
1116	50-51	0.18644191852074243
1116	52-53	0.13827534856871182
1116	54-55	0.21141166244348852
1116	56-57	0.019479150143389745
1116	58-59	0.08910289998146936
1116	60-61	0.17444066079605847
1116	62-63	0.18797131982890392
1116	64-65	0.17974239194884234
1116	66-67	0.16154056504708336
1116	68-69	0.11402101829084899
1116	70-71	0.19413032409202913
1116	72-73	0.25934885947916086
1116	74-75	0.03134045151406539
1116	76	-0.3488449447957649
2118	1	0.043894160570076224
2118	2	0.055988440930200056
2118	3	0.09816973180505073
2118	4	0.01513126172812207
2118	5	0.05054905486473871
2118	6	0.010916259087458968
2118	7	0.07386513813825957
2118	8	0.11212158744244505
2118	9	0.09725990486941072
2118	10-11	0.18281137838695116
2118	12-13	0.10673958352030155
2118	14-15	0.008973318140526487
2118	16-17	-0.10214064500995335
2118	18-19	0.040213680090111836
2118	20-21	0.002217724563735146
2118	22-23	0.24488103544139506
2118	24-25	0.024272870483571296
2118	26-27	0.0599075570323393
2118	28-29	-0.024323513972589694
2118	30-31	0.14105765654238667
2118	32-33	0.0192566393232525
2118	34-35	0.09559508489000024
2118	36-37	0.028246622549652045
2118	38-39	-0.12388004239933537
2118	40-41	-0.16995480158826837
2118	42-43	0.037567450556991844
2118	44-45	-0.08727562825952617
2118	46-47	0.03502289292390515
2118	48-49	-0.2700012981861448
2118	50-51	0.040407660703806414
2118	52-53	-0.03881415432056201
2118	54-55	-0.06269825357478709
2118	56-57	-0.03087699709722358
2118	58-59	-0.07004201509981556
2118	60-61	-0.22861834675833848
2118	62-63	-0.0858289260430567
2118	64-65	-0.4274601697734255
2118	66-67	-0.11078706130733451
2118	68-69	-0.3039864519750921
2118	70-71	0.04119522547670584
2118	72-73	-0.15968810561113855
2118	74-75	-0.4476379942990967
2118	76	-1.0188491947374665
1119	1	0.007990790484782906
1119	2	0.026840518629384746
1119	3	-0.016830528232397057
1119	4	0.035353723762654
1119	5	0.03101861748175594
1119	6	0.04707609110326416
1119	7	0.07660235729781562
1119	8	0.14306051764839367
1119	9	0.09213229149307978
1119	10-11	0.17200227187561268
1119	12-13	0.0648485262080456
1119	14-15	-0.056270239431761127
1119	16-17	0.07321560629023338
1119	18-19	0.019173725336628422
1119	20-21	0.11261629695815145
1119	22-23	0.31497282865962006
1119	24-25	0.14862700247857674
1119	26-27	0.15410693424266242
1119	28-29	0.17781513149235906
1119	30-31	0.2163840035363478
1119	32-33	0.2546030417052023
1119	34-35	0.20499267564306933
1119	36-37	0.2348787150709697
1119	38-39	0.11447141771092362
1119	40-41	0.33988859086024803
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2101	32-33	0.03286886151776258
2101	34-35	0.10709224335698764
2101	36-37	0.16863252961284303
2101	38-39	0.14163050431951518
2101	40-41	-0.18565967965140828
2101	42-43	-0.010024590102247544
2101	44-45	0.05667048705344513
2101	46-47	-0.03407857055392327
2101	48-49	0.13628643794721995
2101	50-51	0.11191971184516092
2101	52-53	0.11176812958886728
2101	54-55	0.11927044764752992
2101	56-57	0.15050364396758908
2101	58-59	0.06366156534262046
2101	60-61	-0.010398427934113386
2101	62-63	-0.09750629998575278
2101	64-65	0.1180535690369311
2101	66-67	0.018235311738180826
2101	68-69	-0.05795654001654782
2101	70-71	-0.005309092247081537
2101	72-73	0.4726571905311303
2101	74-75	0.306052482633671
2101	76	0.2506446465870056
2107	1	0.0330000292135324
2107	2	-0.009921537911630196
2107	3	0.05967076518962955
2107	4	-0.023157455099138247
2107	5	-0.01863608503118286
2107	6	-0.08408960549687805
2107	7	-0.30829578571770355
2107	8	-0.23704965419196355
2107	9	-0.18124977613441473
2107	10-11	-0.002182410312123295
2107	12-13	-0.34131486070991457
2107	14-15	0.05687127479588128
2107	16-17	-0.1203634772278761
2107	18-19	-0.06391593652590188
2107	20-21	-0.01643142107584339
2107	22-23	0.26031189000477895
2107	24-25	0.06673606567147772
2107	26-27	0.026190383060097133
2107	28-29	0.01265841955592606
2107	30-31	0.016587255568872195
2107	32-33	-0.0937218612881523
2107	34-35	-0.03648055514037196
2107	36-37	-0.29032049501771695
2107	38-39	-0.15742494684931785
2107	40-41	-0.0722245373733088
2107	42-43	-0.125504906659188
2107	44-45	-0.041138230815754184
2107	46-47	-0.17942070727972492
2107	48-49	-0.04841191126564581
2107	50-51	-0.32985061990324027
2107	52-53	-0.035728334352732816
2107	54-55	-0.21940151152684706
2107	56-57	0.016598969523272444
2107	58-59	-0.020445337405874398
2107	60-61	-0.02841967180768279
2107	62-63	-0.3177175505253018
2107	64-65	0.04391113452365403
2107	66-67	-0.32348900258199365
2107	68-69	0.013095376100835665
2107	70-71	-0.3068379717915022
2107	72-73	0.17833485506105262
2107	74-75	-0.17362664398172356
2107	76	0.19544554988462082
2106	1	0.051641029579798214
2106	2	0.06167708874184541
2106	3	0.017992319581310312
2106	4	1.437953586957974E-4
2106	5	0.022894002451224083
2106	6	-0.03857288876903908
2106	7	-0.5217709811242912
2106	8	-0.12969898074881314
2106	9	-0.07358164473279061
2106	10-11	0.01181411701357149
2106	12-13	0.106445988058816
2106	14-15	0.06462982539213158
2106	16-17	-0.013766796051704944
2106	18-19	-0.12996777205385968
2106	20-21	-0.15465477867137167
2106	22-23	0.1503598016239991
2106	24-25	0.05891531149397622
2106	26-27	-0.01887117920825432
2106	28-29	0.03220323494235089
2106	30-31	0.011310354323839533
2106	32-33	-0.031122776830962096
2106	34-35	-0.1193832288282124
2106	36-37	-0.32322291562658023
2106	38-39	-0.40595196798378197
2106	40-41	-0.08761373137492967
2106	42-43	-0.14870613169065905
2106	44-45	-0.06151152984234187
2106	46-47	-0.15958299874937865
2106	48-49	-0.11271259105731701
2106	50-51	-0.28503076420994944
2106	52-53	-0.08195235835366788
2106	54-55	-0.4201417513746435
2106	56-57	0.0018459191408481956
2106	58-59	0.009944310522499222
2106	60-61	0.035105972469459346
2106	62-63	-0.22086204063525372
2106	64-65	-0.05008659681179495
2106	66-67	-0.23272952980904194
2106	68-69	0.0822155949678347
2106	70-71	-0.17594620440748798
2106	72-73	0.10113916610524143
2106	74-75	-0.05973684059823725
2106	76	0.10874672388029083
2105	1	0.04998390704611211
2105	2	-0.027422687602147278
2105	3	-0.03849389893107258
2105	4	-0.045543698757256834
2105	5	-0.05206368813895068
2105	6	-0.14366532364544327
2105	7	-0.09954669747156686
2105	8	-0.056297493210081484
2105	9	-0.15624757047376647
2105	10-11	-0.02549283386036194
2105	12-13	0.0778204169011616
2105	14-15	-0.026385902650083892
2105	16-17	-0.03396711952498066
2105	18-19	-0.1540990176706174
2105	20-21	-0.1801535873351554
2105	22-23	0.27567569797365366
2105	24-25	0.08901120220429704
2105	26-27	0.08799829441743157
2105	28-29	0.011647176665341874
2105	30-31	-0.0441846254734628
2105	32-33	-0.10013152728487995
2105	34-35	-0.06526683421833468
2105	36-37	-0.21300489875089568
2105	38-39	-0.1273697261555924
2105	40-41	-0.11205650642877885
2105	42-43	-0.45076998182454986
2105	44-45	-0.2570151740189388
2105	46-47	-0.30561157820381624
2105	48-49	-0.3069589997774358
2105	50-51	-0.547000091950494
2105	52-53	-0.1967933985948207
2105	54-55	-0.8823475114602743
2105	56-57	-0.39807702781506293
2105	58-59	-0.2740747560389707
2105	60-61	-0.17527940891879012
2105	62-63	-0.8733007805238415
2105	64-65	-0.8679824986631814
2105	66-67	-0.9555446362917337
2105	68-69	-0.22841651459147272
2105	70-71	-1.1896423182195193
2105	72-73	-0.6466642158144964
2105	74-75	-0.6350158191486663
2105	76	-0.008022362681376904
2104	1	-0.06423121441794422
2104	2	0.006325585520329469
2104	3	-0.04998229112813135
2104	4	-0.057490542914443665
2104	5	-0.057783425104176445
2104	6	-0.21375416172623574
2104	7	-0.00793049814995328
2104	8	0.09217214272010921
2104	9	0.06566318454581221
2104	10-11	0.003994689598449952
2104	12-13	0.11993600273753913
2104	14-15	-0.05351983032781504
2104	16-17	-0.014965038366320016
2104	18-19	-0.02486680366249061
2104	20-21	0.027413391179194946
2104	22-23	0.2940511549518021
2104	24-25	0.1964166187358103
2104	26-27	0.12974263451125978
2104	28-29	0.1330227721198085
2104	30-31	-0.030043263975258583
2104	32-33	0.02489594220329394
2104	34-35	3.176036016583339E-4
2104	36-37	-0.14823250415658862
2104	38-39	-0.06053106788079532
2104	40-41	0.19261467376328767
2104	42-43	-0.12936819001116362
2104	44-45	-0.12449885099682234
2104	46-47	0.05745136129231554
2104	48-49	-0.08037018465060441
2104	50-51	0.01097467048861489
2104	52-53	-0.19520844675673743
2104	54-55	-0.017056878003849363
2104	56-57	-0.176225225578321
2104	58-59	-0.01821296795340288
2104	60-61	-0.058984884829555995
2104	62-63	-0.10277116377463358
2104	64-65	-0.6799537580526618
2104	66-67	-0.11156239211733521
2104	68-69	-0.49061770237716473
2104	70-71	-0.1643910539331941
2104	72-73	-0.7816770010424818
2104	74-75	0.11688456143544457
2104	76	-0.14364537192743043
2111	1	0.009884512247076316
2111	2	-0.07283576790008084
2111	3	-0.021605312124172826
2111	4	-0.09122881195069965
2111	5	-0.06873291518910918
2111	6	-0.1742465189748188
2111	7	-0.02580719226996564
2111	8	0.03130698059007386
2111	9	-0.006221637955093229
2111	10-11	-0.04504195849184356
2111	12-13	0.03757959388713772
2111	14-15	-0.09818746133116463
2111	16-17	-0.14761141572538605
2111	18-19	-0.012768465713492105
2111	20-21	0.010045400729282505
2111	22-23	0.27881110005498044
2111	24-25	0.09318687784093527
2111	26-27	0.021461024466212564
2111	28-29	0.032165635690468264
2111	30-31	-0.11014507640410898
2111	32-33	-0.047394347531181324
2111	34-35	-0.18724919826333064
2111	36-37	-0.11666413687204624
2111	38-39	0.0312842172264709
2111	40-41	-0.38684627340289524
2111	42-43	-0.15847002848089176
2111	44-45	-0.12936561150955583
2111	46-47	0.07815804405817772
2111	48-49	-0.17305901359052456
2111	50-51	0.07368686040641137
2111	52-53	-0.12945930087292368
2111	54-55	0.05943870501769766
2111	56-57	-0.10658107257199845
2111	58-59	0.12354864491989304
2111	60-61	-0.23942318087910053
2111	62-63	0.053185448820947556
2111	64-65	-0.3284337328622371
2111	66-67	0.13341598241415653
2111	68-69	-0.15601024064282853
2111	70-71	0.14719618639760768
2111	72-73	-0.33830199076210477
2111	74-75	0.4674046497502964
2111	76	0.08756872812196548
2110	1	0.01989338054593759
2110	2	0.026684822678930686
2110	3	0.07689313434019596
2110	4	0.0448289344620747
2110	5	0.03113625263772235
2110	6	0.043206978481855174
2110	7	0.013516429834943722
2110	8	0.10649236473714296
2110	9	0.09172398107116919
2110	10-11	-0.05770843396744141
2110	12-13	-0.005784840742983022
2110	14-15	-0.0994284101368379
2110	16-17	-0.15063004353761045
2110	18-19	-0.22152844011380068
2110	20-21	-0.08785994475519487
2110	22-23	0.20817680882304046
2110	24-25	-0.06851713712264029
2110	26-27	-0.04910016947541607
2110	28-29	-0.03043952412971862
2110	30-31	-0.2104621858875646
2110	32-33	-0.012757538553294978
2110	34-35	0.005240833629088115
2110	36-37	0.012253459726402127
2110	38-39	0.16865669990681909
2110	40-41	-0.2968067525541116
2110	42-43	0.00939845696923669
2110	44-45	0.00576921310322831
2110	46-47	0.17981382252381906
2110	48-49	0.014290092572316837
2110	50-51	0.1942754663393771
2110	52-53	-0.020696541427270176
2110	54-55	0.1970687028952156
2110	56-57	-0.016483983293511528
2110	58-59	0.08608024462881758
2110	60-61	0.02812629914562592
2110	62-63	0.16052627211282555
2110	64-65	0.1303716913667543
2110	66-67	0.24875272768514378
2110	68-69	0.12355890228910482
2110	70-71	0.257554703740162
2110	72-73	-0.02240325095046103
2110	74-75	0.5916673638070087
2110	76	0.14750631387578395
2109	1	0.028267950771180494
2109	2	-0.07326606594682517
2109	3	0.0083521881839701
2109	4	-0.03802905016657121
2109	5	0.028487284281972336
2109	6	0.02822427233985536
2109	7	-0.010654868966824438
2109	8	0.08611701110872616
2109	9	0.036613601429237974
2109	10-11	-0.011098075140182573
2109	12-13	0.08070725297672965
2109	14-15	-0.09147099727677244
2109	16-17	-0.020011769830944104
2109	18-19	-0.1068994306976947
2109	20-21	-0.24859188505388374
2109	22-23	0.07376793425063966
2109	24-25	-0.11274604434593982
2109	26-27	-0.21833659087559454
2109	28-29	-0.10982087591968792
2109	30-31	-0.5988026224620455
2109	32-33	-0.0880459141779184
2109	34-35	-0.09933999343827793
2109	36-37	-0.09019008630287573
2109	38-39	-0.05382815239660488
2109	40-41	-0.563657133478678
2109	42-43	-0.14649025477616817
2109	44-45	-0.11152319028092705
2109	46-47	0.0396817369563891
2109	48-49	0.02269459895678949
2109	50-51	0.08158398944117096
2109	52-53	-0.0636079964564118
2109	54-55	0.03604435262238326
2109	56-57	0.014854092302485356
2109	58-59	0.09474481386234856
2109	60-61	-0.09247780692208352
2109	62-63	0.07870827769244215
2109	64-65	0.20676063271646683
2109	66-67	0.1625711663661633
2109	68-69	0.011752169490179654
2109	70-71	0.11792679131351491
2109	72-73	-0.6586625418082548
2109	74-75	0.41645958275228523
2109	76	-0.31859815841763606
2108	1	0.01625115121287024
2108	2	-0.041397045467455484
2108	3	-0.08571796971612855
2108	4	0.01669640109724213
2108	5	0.028447867803834015
2108	6	-0.07039920689920365
2108	7	-0.13496965358908142
2108	8	0.052256401260009966
2108	9	0.01377081481030018
2108	10-11	0.022564036037501012
2108	12-13	0.08803792997987614
2108	14-15	-0.11860630020258611
2108	16-17	-0.016227388977441137
2108	18-19	-0.016033317821907644
2108	20-21	0.04382242968645045
2108	22-23	0.13645603273630513
2108	24-25	0.049808588668099674
2108	26-27	0.007219387923925069
2108	28-29	-0.052034832871711956
2108	30-31	-0.026554182873844923
2108	32-33	0.012782494123655397
2108	34-35	-0.24554023896144628
2108	36-37	-0.45605123322105356
2108	38-39	-0.35102906580566895
2108	40-41	-0.7520380174602224
2108	42-43	-0.20290425131223344
2108	44-45	-0.30526537356045225
2108	46-47	-0.3414161627637
2108	48-49	-0.30323475322979476
2108	50-51	-0.18750787844069805
2108	52-53	-0.1792558834718676
2108	54-55	-0.2883792940888341
2108	56-57	-0.2982078543233797
2108	58-59	-0.1658048588990937
2108	60-61	-0.6026881309355119
2108	62-63	-0.1928068059524719
2108	64-65	-0.05975976876190714
2108	66-67	-0.2048851610338005
2108	68-69	-0.6484436885161884
2108	70-71	-0.2874359103014754
2108	72-73	-1.915469723698024
2108	74-75	-0.1548599664365078
2108	76	-0.44012983249012194
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	0.0
14	2.0
15	2.0
16	5.0
17	5.0
18	38.0
19	90.0
20	195.0
21	365.0
22	591.0
23	903.0
24	1374.0
25	2308.0
26	3875.0
27	6266.0
28	9064.0
29	13002.0
30	20014.0
31	29267.0
32	42988.0
33	64154.0
34	100391.0
35	200690.0
36	560597.0
37	1590822.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	65.41097313572672	7.8244509843181556	7.420636869524483	19.343939010430635
2	15.646068583042755	55.91184015171835	15.656230992704975	12.785860272533917
3	15.182413364513167	13.152424811390967	57.13514493711773	14.530016886978137
4	16.311196406511495	16.29778504803533	10.196599181718241	57.19441936373493
5	61.04831489114133	16.840359499964112	11.764556990717828	10.346768618176736
6	56.9552060626896	19.3901420848429	12.800858326942475	10.853793525525028
7	10.398260679030303	56.05592725376935	21.540795085776026	12.005016981424324
8	12.322469503326394	57.32668180324215	15.922493681550126	14.42835501188133
9	56.9428525015017	12.504448415381884	17.241642456960875	13.311056626155548
10-11	35.91204793332855	39.49922743019482	12.373508222484993	12.215216413991637
12-13	12.597799026070925	36.538169481792664	15.522230743367043	35.341800748769366
14-15	12.636389737855163	36.69595128087918	14.945598996603715	35.72205998466194
16-17	35.837102209115606	14.400571663985339	14.37250225206974	35.38982387482931
18-19	13.092281479858405	14.542427115877915	37.18284026127593	35.182451142987745
20-21	35.55264241540455	14.569967623847285	36.881235809460485	12.996154151287678
22-23	13.119935323251516	59.201891946007	14.31447180025765	13.36370093048383
24-25	13.186406549276352	14.727447195137156	37.03467308397022	35.05147317161628
26-27	13.268820291574269	14.744806404207011	14.127317237184597	57.85905606703412
28-29	35.64467077948327	36.7143682872373	14.4794503987518	13.161510534527638
30-31	12.783706899482814	14.80740533658732	36.51569128941712	35.89319647451275
32-33	13.015458951798445	36.78044283927904	14.54524161223418	35.65885659668834
34-35	35.000188892372904	14.722101540983978	14.366889433738445	35.910820132904675
36-37	13.038806049089349	37.26387508925165	14.456556643155864	35.24076221850314
38-39	12.971465918149159	37.04870778727696	36.6179953985818	13.361830895992084
40-41	12.851953713812945	36.49332643246531	36.49544202704183	14.159277826679913
42-43	12.626737337599783	14.352363610262145	37.15571531652695	35.865183735611126
44-45	35.05135983619254	14.505951998670197	15.222326322907733	35.220361842229536
46-47	13.176735259783682	36.84583737877832	36.759456896649425	13.217970464788573
48-49	35.45721398861357	14.553873993675884	36.61831651561573	13.370595502094817
50-51	13.599023048647341	14.491350618244736	14.519476692570107	57.390149640537814
52-53	35.11484656272549	14.395582185182526	37.09823536745233	13.391335884639648
54-55	12.720427954560051	14.40980578086218	37.67443266175798	35.19533360281979
56-57	12.663949135061825	14.548887235031223	37.26950408196418	35.51765954794278
58-59	35.49310353946528	14.411222473658958	36.58110471815369	13.514569268722068
60-61	13.446813574561487	14.739196300731768	37.06342250312617	34.75056762158057
62-63	35.481071095311314	14.898980358971064	36.94572366556983	12.674224880147788
64-65	13.12658433477773	59.59986928647795	14.38421086433372	12.889335514410599
66-67	12.779966830499317	59.9250097279572	14.599680394105047	12.695343047438431
68-69	12.917140471702035	59.77304581395613	14.373840673061302	12.935973041280539
70-71	13.241789793011737	59.75772664251363	14.141994174559219	12.858489389915414
72-73	13.041393874598132	59.487818330871434	14.498245189855725	12.972542604674707
74-75	13.34220497844738	53.3344981696329	17.51430859724746	15.80898825467225
76	13.867155771984239	51.146727817424186	18.495698920668982	16.490417489922592
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	394.0
1	334.5
2	268.5
3	251.0
4	240.0
5	206.5
6	152.0
7	125.5
8	120.0
9	131.0
10	135.5
11	138.0
12	147.0
13	173.0
14	207.5
15	278.5
16	367.5
17	394.0
18	483.0
19	726.0
20	1007.0
21	1134.0
22	1399.5
23	2070.0
24	3099.5
25	3724.0
26	4278.5
27	5772.0
28	7659.5
29	8608.0
30	10078.5
31	13285.0
32	17202.5
33	19384.0
34	22270.0
35	28124.0
36	35695.0
37	40298.0
38	45327.5
39	57987.0
40	71582.5
41	100399.0
42	123250.0
43	537239.5
44	636837.5
45	234925.5
46	147405.0
47	123240.0
48	91613.5
49	78111.0
50	72070.0
51	67256.0
52	57263.0
53	51460.5
54	50837.0
55	48537.5
56	42010.0
57	30677.5
58	23573.0
59	24834.5
60	25496.0
61	26183.5
62	27471.0
63	27487.5
64	26916.0
65	23722.5
66	17659.0
67	14201.0
68	9834.5
69	5882.0
70	8134.5
71	9973.0
72	8765.0
73	6607.0
74	3497.5
75	1338.0
76	900.5
77	308.0
78	103.0
79	53.0
80	40.5
81	21.5
82	11.0
83	7.0
84	9.0
85	8.5
86	3.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.888923729037669E-5
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	2647010.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.75319218065319
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.91040180173232	25.190611709033938
2	15.473223279346998	10.13594911445218
3	3.979122953337439	3.9098693640332827
4	1.338083516056911	1.7530602622070457
5	0.5489074949908196	0.8989236336417622
6	0.4284006912419164	0.8418894102342694
7	0.20878454063120827	0.478685212855035
8	0.1679510696723988	0.4400746929541081
9	0.1179650504186497	0.3477358770266229
>10	0.6773633149301526	4.162002793378083
>50	0.07337715752541885	1.6786875332952005
>100	0.06317199980431899	4.472863530610405
>500	0.007407673302176336	1.6722905110287893
>1k	0.005255896550316123	3.160063419281451
>5k	4.668483670895212E-4	1.0590772540192
>10k+	1.1671209177237772E-4	39.79821568194863
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC	1041104	39.33132099992066	TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATACCTCGTATGC	9634	0.36395782411097805	TruSeq Adapter, Index 6 (98% over 50bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC	7061	0.2667538090146996	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAATATCTCGTATGC	5892	0.2225907722297989	TruSeq Adapter, Index 6 (98% over 50bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG	5118	0.1933502329042958	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAACTCGTATGC	4924	0.18602120883562964	TruSeq Adapter, Index 6 (98% over 50bp)
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG	4065	0.15356949917076249	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAACATCTCGTATGC	3983	0.1504716642551407	TruSeq Adapter, Index 6 (98% over 50bp)
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG	3473	0.13120464221895647	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAATATCTCGTATGC	3343	0.12629344052345856	TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGGACTCCAGTCACGCCAATATCTCGTATGC	3205	0.12108001103131458	TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATCTCGTATGCCG	3118	0.11779328374278902	TruSeq Adapter, Index 6 (97% over 42bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.019304800510764976	0.0	0.0
2	0.020513711697349085	0.0	0.0
3	0.02327154034174408	0.0	0.0
4	0.02527379949452401	0.0	0.0
5	0.029127203901760854	0.0	0.0
6	0.031053906105379277	0.0	0.0
7	0.033245057631062974	0.0	0.0
8	0.037816253055334134	0.0	0.0
9	0.042651897801670564	0.0	0.0
10	0.05526990831164219	3.777847458075338E-5	0.0
11	0.06010555305797863	3.777847458075338E-5	3.777847458075338E-5
12	0.06312783102443889	3.777847458075338E-5	3.777847458075338E-5
13	0.07208132950007745	3.777847458075338E-5	3.777847458075338E-5
14	0.083943770518434	3.777847458075338E-5	3.777847458075338E-5
15	0.08689049153573276	3.777847458075338E-5	3.777847458075338E-5
16	0.10555305797862494	3.777847458075338E-5	3.777847458075338E-5
17	0.10710197543643583	3.777847458075338E-5	3.777847458075338E-5
18	0.10872644984340822	3.777847458075338E-5	3.777847458075338E-5
19	0.12191113747209115	3.777847458075338E-5	3.777847458075338E-5
20	0.12296893476035224	3.777847458075338E-5	3.777847458075338E-5
21	0.12546231408268196	3.777847458075338E-5	3.777847458075338E-5
22	0.12712456696423513	3.777847458075338E-5	3.777847458075338E-5
23	0.12874904137120752	3.777847458075338E-5	3.777847458075338E-5
24	0.1302979588290184	3.777847458075338E-5	3.777847458075338E-5
25	0.13203576865973304	3.777847458075338E-5	3.777847458075338E-5
26	0.1336980215412862	3.777847458075338E-5	3.777847458075338E-5
27	0.1358136161178084	3.777847458075338E-5	3.777847458075338E-5
28	0.1397803559487875	3.777847458075338E-5	3.777847458075338E-5
29	0.1463538105258386	3.777847458075338E-5	3.777847458075338E-5
30	0.1838678357845267	3.777847458075338E-5	3.777847458075338E-5
31	0.18583231646272586	3.777847458075338E-5	3.777847458075338E-5
32	0.18908126527667066	3.777847458075338E-5	3.777847458075338E-5
33	0.19127241680235435	3.777847458075338E-5	3.777847458075338E-5
34	0.19361468222636105	3.777847458075338E-5	7.555694916150676E-5
35	0.19561694137914099	3.777847458075338E-5	7.555694916150676E-5
36	0.19780809290482468	3.777847458075338E-5	7.555694916150676E-5
37	0.2002259152779929	3.777847458075338E-5	7.555694916150676E-5
38	0.20324819324445317	3.777847458075338E-5	7.555694916150676E-5
39	0.2052504523972331	3.777847458075338E-5	7.555694916150676E-5
40	0.20778161019414357	3.777847458075338E-5	7.555694916150676E-5
41	0.21087944510976536	3.777847458075338E-5	7.555694916150676E-5
42	0.21431728629661392	3.777847458075338E-5	7.555694916150676E-5
43	0.21718845036475118	3.777847458075338E-5	7.555694916150676E-5
44	0.2203240637549537	3.777847458075338E-5	7.555694916150676E-5
45	0.22364856951806	3.777847458075338E-5	7.555694916150676E-5
46	0.22784198019652363	3.777847458075338E-5	7.555694916150676E-5
47	0.23297985273950608	3.777847458075338E-5	7.555694916150676E-5
48	0.23917552257074964	3.777847458075338E-5	7.555694916150676E-5
49	0.24355782562211703	3.777847458075338E-5	7.555694916150676E-5
50	0.24869569816509948	3.777847458075338E-5	7.555694916150676E-5
51	0.2540980200301472	3.777847458075338E-5	7.555694916150676E-5
52	0.2593114495222912	3.777847458075338E-5	7.555694916150676E-5
53	0.2638070879974008	3.777847458075338E-5	7.555694916150676E-5
54	0.27045609952361344	3.777847458075338E-5	7.555694916150676E-5
55	0.27650065545653396	3.777847458075338E-5	7.555694916150676E-5
56	0.28243187596571223	3.777847458075338E-5	7.555694916150676E-5
57	0.2913098174921893	7.555694916150676E-5	7.555694916150676E-5
58	0.30003664512034334	7.555694916150676E-5	1.1333542374226014E-4
59	0.3090279220705626	7.555694916150676E-5	1.1333542374226014E-4
60	0.31919033173278527	7.555694916150676E-5	1.1333542374226014E-4
61	0.3305238741070113	7.555694916150676E-5	1.1333542374226014E-4
62	0.3421218658033026	7.555694916150676E-5	1.1333542374226014E-4
63	0.35360652207585164	7.555694916150676E-5	1.1333542374226014E-4
64	0.36754677919614964	7.555694916150676E-5	1.1333542374226014E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGAAG	120610	0.0	68.84504	3
GATCGGA	121130	0.0	68.737305	1
ATCGGAA	121110	0.0	68.644615	2
CGGAAGA	121080	0.0	68.56335	4
AGCACAC	121125	0.0	68.53787	10
GCACACG	120965	0.0	68.53594	11
CACACGT	121015	0.0	68.45557	12
GAGCACA	121280	0.0	68.43585	9
ACACGTC	120955	0.0	68.42876	13
ACGTCTG	120840	0.0	68.36933	15
CACGTCT	120925	0.0	68.364685	14
AGAGCAC	121430	0.0	68.34555	8
CGTCTGA	117985	0.0	68.16968	16
CTGAACT	117970	0.0	68.11308	19
GTCTGAA	118080	0.0	68.094086	17
TCTGAAC	118195	0.0	68.01599	18
ACTCCAG	120775	0.0	67.95115	23
AAGAGCA	122275	0.0	67.94479	7
GAAGAGC	122290	0.0	67.899254	6
AGTCACG	119925	0.0	67.89577	28
>>END_MODULE