FastQCFastQC Report
Mon 15 Sep 2014
TGA3_5_GCCAAT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameTGA3_5_GCCAAT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2647010
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC104110439.33132099992066TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATACCTCGTATGC96340.36395782411097805TruSeq Adapter, Index 6 (98% over 50bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC70610.2667538090146996No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACAATATCTCGTATGC58920.2225907722297989TruSeq Adapter, Index 6 (98% over 50bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG51180.1933502329042958No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAACTCGTATGC49240.18602120883562964TruSeq Adapter, Index 6 (98% over 50bp)
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG40650.15356949917076249No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAACATCTCGTATGC39830.1504716642551407TruSeq Adapter, Index 6 (98% over 50bp)
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG34730.13120464221895647No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAATATCTCGTATGC33430.12629344052345856TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGGACTCCAGTCACGCCAATATCTCGTATGC32050.12108001103131458TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATCTCGTATGCCG31180.11779328374278902TruSeq Adapter, Index 6 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG1206100.068.845043
GATCGGA1211300.068.7373051
ATCGGAA1211100.068.6446152
CGGAAGA1210800.068.563354
AGCACAC1211250.068.5378710
GCACACG1209650.068.5359411
CACACGT1210150.068.4555712
GAGCACA1212800.068.435859
ACACGTC1209550.068.4287613
ACGTCTG1208400.068.3693315
CACGTCT1209250.068.36468514
AGAGCAC1214300.068.345558
CGTCTGA1179850.068.1696816
CTGAACT1179700.068.1130819
GTCTGAA1180800.068.09408617
TCTGAAC1181950.068.0159918
ACTCCAG1207750.067.9511523
AAGAGCA1222750.067.944797
GAAGAGC1222900.067.8992546
AGTCACG1199250.067.8957728
Produced by FastQC (version 0.11.2)