##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	TGA3_4+8_GATCAG_L001_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1699152
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.3591161944311	34.0	34.0	34.0	34.0	34.0
2	33.42920292004482	34.0	34.0	34.0	34.0	34.0
3	33.4286032091302	34.0	34.0	34.0	34.0	34.0
4	33.472653417704834	34.0	34.0	34.0	34.0	34.0
5	33.5388570298596	34.0	34.0	34.0	34.0	34.0
6	37.18076311006902	38.0	38.0	38.0	36.0	38.0
7	37.00017126190006	38.0	38.0	38.0	36.0	38.0
8	37.00310919799994	38.0	38.0	38.0	36.0	38.0
9	37.11780935431321	38.0	38.0	38.0	36.0	38.0
10-11	37.222457437592396	38.0	38.0	38.0	37.0	38.0
12-13	37.1202420383815	38.0	38.0	38.0	36.5	38.0
14-15	37.03777825644792	38.0	38.0	38.0	36.0	38.0
16-17	37.12027646732017	38.0	38.0	38.0	36.5	38.0
18-19	37.182733210448504	38.0	38.0	38.0	37.0	38.0
20-21	37.20892686469486	38.0	38.0	38.0	37.0	38.0
22-23	37.29655233904913	38.0	38.0	38.0	37.0	38.0
24-25	37.18748322692731	38.0	38.0	38.0	37.0	38.0
26-27	37.22325783685038	38.0	38.0	38.0	37.0	38.0
28-29	37.16585567388909	38.0	38.0	38.0	37.0	38.0
30-31	37.17587214092677	38.0	38.0	38.0	37.0	38.0
32-33	37.18368839279829	38.0	38.0	38.0	37.0	38.0
34-35	37.188504030245674	38.0	38.0	38.0	37.0	38.0
36-37	37.03936463600667	38.0	38.0	38.0	36.0	38.0
38-39	37.04161222774655	38.0	38.0	38.0	36.0	38.0
40-41	37.189643716395	38.0	38.0	38.0	37.0	38.0
42-43	37.03689340329765	38.0	38.0	38.0	36.0	38.0
44-45	36.99870965046094	38.0	38.0	38.0	36.0	38.0
46-47	36.94621640677232	38.0	38.0	38.0	36.0	38.0
48-49	36.79731948642617	38.0	38.0	38.0	35.5	38.0
50-51	36.88582216305545	38.0	38.0	38.0	35.0	38.0
52-53	36.82601085718052	38.0	38.0	38.0	35.5	38.0
54-55	36.97172030518753	38.0	38.0	38.0	36.0	38.0
56-57	36.869856551974166	38.0	38.0	38.0	36.0	38.0
58-59	37.026381394954655	38.0	38.0	38.0	36.0	38.0
60-61	37.0253614744296	38.0	38.0	38.0	36.0	38.0
62-63	37.03565602135653	38.0	38.0	38.0	36.0	38.0
64-65	36.87720521766151	38.0	38.0	38.0	36.0	38.0
66-67	36.77819641797791	38.0	38.0	38.0	35.5	38.0
68-69	32.90786698305979	38.0	32.0	38.0	16.0	38.0
70-71	32.25112232454777	38.0	28.5	38.0	11.0	38.0
72-73	32.38605845739522	38.0	31.5	38.0	11.0	38.0
74-75	32.44029698343644	38.0	33.5	38.0	11.0	38.0
76	32.13684532048928	38.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.08568219513028907
1101	2	0.11118731636346979
1101	3	0.1520261656421411
1101	4	0.13290491302155516
1101	5	0.05037779275305354
1101	6	0.10832239923141174
1101	7	0.17463967955556114
1101	8	0.2777456017502118
1101	9	0.1739119319644047
1101	10-11	0.21149299867155236
1101	12-13	0.18825102310087516
1101	14-15	0.2751925226870924
1101	16-17	0.07603471740370082
1101	18-19	0.02545429908106911
1101	20-21	0.07587148861777138
1101	22-23	0.10082389887406862
1101	24-25	0.0507323206020871
1101	26-27	0.05736716695961519
1101	28-29	0.1469457049475693
1101	30-31	0.04053225277396422
1101	32-33	0.11397613851063682
1101	34-35	0.1105193535531086
1101	36-37	0.12301855687629626
1101	38-39	0.14978082017231742
1101	40-41	0.11579680860513264
1101	42-43	0.06653159439090217
1101	44-45	0.10339876216413302
1101	46-47	0.20704616957699074
1101	48-49	0.16860368131129633
1101	50-51	0.11301678754031741
1101	52-53	0.06638814704806606
1101	54-55	0.07028511588239184
1101	56-57	0.2326908756511301
1101	58-59	0.07710455754874346
1101	60-61	0.2836846284458474
1101	62-63	0.20685867060412733
1101	64-65	0.3380269078606446
1101	66-67	0.1730929513615891
1101	68-69	0.14898555884467868
1101	70-71	-0.026880968040124742
1101	72-73	-0.05880007266620879
1101	74-75	-0.08328209316977109
1101	76	-0.146176928398134
1102	1	0.0016750269721441668
1102	2	0.034452257898827554
1102	3	0.09638142007842987
1102	4	0.03958457629207146
1102	5	0.04527275114171658
1102	6	0.15541883516385724
1102	7	-0.03553931978430569
1102	8	0.2572747897628602
1102	9	0.07416546441839955
1102	10-11	0.1740368715211602
1102	12-13	0.1576985556979622
1102	14-15	0.10635559538260253
1102	16-17	-0.051019637742861335
1102	18-19	0.00531198172197378
1102	20-21	0.0676710224369188
1102	22-23	0.0330579627366987
1102	24-25	0.04596322291553889
1102	26-27	0.0020972080811745286
1102	28-29	0.15134797315194248
1102	30-31	-0.02921229391771618
1102	32-33	0.08027542425369916
1102	34-35	0.08934008444228425
1102	36-37	0.11516987646837862
1102	38-39	0.08629665744930293
1102	40-41	0.021394122949921268
1102	42-43	0.08606171501677551
1102	44-45	0.1087473087023767
1102	46-47	0.06822642451029282
1102	48-49	0.10114016726431174
1102	50-51	0.03493877570144832
1102	52-53	0.08936424452897995
1102	54-55	0.11677211228877837
1102	56-57	0.27990393660915913
1102	58-59	0.13936323843408616
1102	60-61	0.2987977515764868
1102	62-63	0.15634199175011787
1102	64-65	0.3750764678853571
1102	66-67	0.24302281139753035
1102	68-69	0.12064359762967314
1102	70-71	-0.11771055507869477
1102	72-73	-0.11115699381004163
1102	74-75	-0.08797952765623762
1102	76	-0.016235946659215017
1103	1	-0.02532801856542477
1103	2	0.13228661329281266
1103	3	0.025096671163240103
1103	4	0.07989764339809824
1103	5	0.07468430766897427
1103	6	-0.020569440794481864
1103	7	-0.10728881301393756
1103	8	0.24750523019596216
1103	9	0.05365895947259247
1103	10-11	-0.04143378101820616
1103	12-13	0.09867157939180515
1103	14-15	-0.18521473903669516
1103	16-17	-0.03540749747118355
1103	18-19	-0.06772149363158775
1103	20-21	0.06297361497781395
1103	22-23	-0.01766051083751563
1103	24-25	-0.05559943376055543
1103	26-27	-0.0935591898087651
1103	28-29	0.09302103793794458
1103	30-31	3.853815250991488E-4
1103	32-33	0.019278310534630805
1103	34-35	-0.0028750673528890047
1103	36-37	0.1464786269328826
1103	38-39	-0.08340786249021903
1103	40-41	0.10035174888555076
1103	42-43	0.10289430679087985
1103	44-45	0.0856332465372418
1103	46-47	0.07842718127427872
1103	48-49	0.1145752347910971
1103	50-51	-0.005577687699997114
1103	52-53	-0.028657242338248068
1103	54-55	-0.05390598677671932
1103	56-57	0.1363050952504068
1103	58-59	0.08230612919778935
1103	60-61	0.23369127572942716
1103	62-63	0.05717990780810567
1103	64-65	0.3194824799220157
1103	66-67	0.07387345324112715
1103	68-69	0.05632876002230347
1103	70-71	-0.07195034544976409
1103	72-73	0.03618941691874511
1103	74-75	0.044404156008951645
1103	76	0.1293259048860662
2116	1	0.002547796716790174
2116	2	0.1276630928251805
2116	3	0.018076080378186532
2116	4	0.08474569981184743
2116	5	0.08820727714837773
2116	6	0.09293738304249644
2116	7	-0.2684433185693962
2116	8	0.23605350906078115
2116	9	0.15020335562557108
2116	10-11	0.04577988837721847
2116	12-13	0.02866041320127266
2116	14-15	-0.0449607191111312
2116	16-17	0.15976788881210524
2116	18-19	-0.007680407092884423
2116	20-21	0.10271950777850236
2116	22-23	-0.029047358490529973
2116	24-25	-0.14851200583412805
2116	26-27	-0.12945000744737456
2116	28-29	0.08603382935951487
2116	30-31	-0.01285702711383152
2116	32-33	-0.11290693671188734
2116	34-35	-0.07115421338900774
2116	36-37	0.060329042646806386
2116	38-39	-0.1581408048371813
2116	40-41	0.10786868484020573
2116	42-43	-0.10183791797198438
2116	44-45	-0.24900999294773385
2116	46-47	-0.03198857629089957
2116	48-49	0.11059312784779962
2116	50-51	0.0022752036791047203
2116	52-53	0.07183951495735386
2116	54-55	-0.1296473772219926
2116	56-57	0.213389071015456
2116	58-59	-0.03378790050243197
2116	60-61	0.11591439008324045
2116	62-63	-0.16047038242557932
2116	64-65	0.15513045837121808
2116	66-67	-0.1697125327291289
2116	68-69	0.03288865696170973
2116	70-71	-0.22801608876023494
2116	72-73	-0.19006562798843873
2116	74-75	-0.1435096669882583
2116	76	-0.18300926895352987
1117	1	-0.15628986971748304
1117	2	0.05378129589038849
1117	3	0.005957069934012793
1117	4	0.14333929746513974
1117	5	-0.15090587583006965
1117	6	-0.3413596585042171
1117	7	-1.1524022391355615
1117	8	-0.12708424156350873
1117	9	-0.11490363133810177
1117	10-11	-0.1857720598274355
1117	12-13	-0.052365874428673465
1117	14-15	-0.4246375172590291
1117	16-17	-0.08012428115544168
1117	18-19	-0.2832905510485162
1117	20-21	0.05234109575395962
1117	22-23	-0.18822619633906612
1117	24-25	-0.05845801680896301
1117	26-27	-0.12020894610677857
1117	28-29	0.06670011711995016
1117	30-31	-0.08586637078952464
1117	32-33	-0.03869813717709292
1117	34-35	-0.11928022215624878
1117	36-37	0.11472553076742997
1117	38-39	-0.37471200729186904
1117	40-41	-0.012820496023643102
1117	42-43	-0.32240307058642514
1117	44-45	-0.04977302011343454
1117	46-47	-0.23715015847126608
1117	48-49	0.1613490097437662
1117	50-51	-0.2406666453161037
1117	52-53	0.021712611558534434
1117	54-55	-0.3075780321235726
1117	56-57	0.10527607613370549
1117	58-59	-0.296762067053578
1117	60-61	0.05340783822389028
1117	62-63	-0.6505898011847862
1117	64-65	0.008380178171961461
1117	66-67	-0.37384010795372546
1117	68-69	0.32048807663434786
1117	70-71	-0.2987814282179997
1117	72-73	-0.09782662278137622
1117	74-75	-0.08853495978242876
1117	76	0.003315264182823796
2117	1	-0.011366850952164498
2117	2	0.1160060302631436
2117	3	0.010221023374079152
2117	4	0.02700230748008181
2117	5	-0.04559974836526948
2117	6	-0.2885496205826854
2117	7	0.10603311197849052
2117	8	0.2006610414571952
2117	9	0.04723836851794516
2117	10-11	0.07877782363422625
2117	12-13	0.010234178939626304
2117	14-15	-0.032667383415009965
2117	16-17	0.1855588252618432
2117	18-19	-0.00795706745429925
2117	20-21	0.1136222782206815
2117	22-23	0.04006303955213042
2117	24-25	0.10726495978447304
2117	26-27	0.03883094825676636
2117	28-29	0.20835693652931297
2117	30-31	0.18953894349142786
2117	32-33	0.08311283390942492
2117	34-35	0.12283915176494986
2117	36-37	0.24354143237470538
2117	38-39	-0.014213296538095221
2117	40-41	0.19722779292356307
2117	42-43	-0.09183461238212942
2117	44-45	0.16510843048216373
2117	46-47	0.022213164165655996
2117	48-49	0.2685014004231121
2117	50-51	0.09166682891525824
2117	52-53	0.24749994025477662
2117	54-55	-0.12141668938030392
2117	56-57	0.3283285477432685
2117	58-59	0.18801628870737375
2117	60-61	0.23005705226061934
2117	62-63	-0.18600810838371018
2117	64-65	0.29905565776005005
2117	66-67	0.05335932036567925
2117	68-69	0.27154733787742913
2117	70-71	-0.14156009861003582
2117	72-73	-0.039570448512037615
2117	74-75	-0.23187209547698018
2117	76	0.018474513741843168
1116	1	0.03603416710979701
1116	2	0.003099425056497296
1116	3	-0.007172692661534086
1116	4	-0.07870683733067096
1116	5	-0.017007116219311058
1116	6	0.024088318525919306
1116	7	0.2776111861980368
1116	8	0.3199291657667658
1116	9	0.12709235926298135
1116	10-11	0.09579070459633954
1116	12-13	0.1637117885345134
1116	14-15	0.01995147582709933
1116	16-17	0.16829889501418194
1116	18-19	0.11188157652657083
1116	20-21	0.22294227115319387
1116	22-23	0.1327817700315208
1116	24-25	0.15107487021347055
1116	26-27	0.088488245553215
1116	28-29	0.18638968150487756
1116	30-31	0.13614561210217602
1116	32-33	0.21728932716990101
1116	34-35	0.09079076137705044
1116	36-37	0.26552911196428397
1116	38-39	0.16804526164787603
1116	40-41	0.239770696237386
1116	42-43	0.040902486524366566
1116	44-45	0.13043826864142716
1116	46-47	0.10199444736778673
1116	48-49	0.3715502700553657
1116	50-51	0.11237714251930697
1116	52-53	0.2772024374070625
1116	54-55	0.14402740907352296
1116	56-57	0.36092634916099087
1116	58-59	0.1563066138351843
1116	60-61	0.286882199219761
1116	62-63	0.2025927952225075
1116	64-65	0.4342408704530669
1116	66-67	0.13344198806016294
1116	68-69	0.2890696602658025
1116	70-71	-0.10190497472186166
1116	72-73	0.06645640089813298
1116	74-75	0.0795654850974401
1116	76	0.16774661742122987
2118	1	0.07546202203935337
2118	2	-0.04359893321758079
2118	3	0.07296364029809155
2118	4	0.049287508350779774
2118	5	0.055747751159017866
2118	6	0.16841006383508983
2118	7	0.4441547045965706
2118	8	0.4116807680812826
2118	9	0.12854002015921395
2118	10-11	0.1955625484043111
2118	12-13	0.14400326057143786
2118	14-15	0.22274804122884007
2118	16-17	0.1621597111176527
2118	18-19	0.25694315264210843
2118	20-21	0.24756147253419414
2118	22-23	0.19801161443472637
2118	24-25	0.19052957404138482
2118	26-27	0.13630033774222738
2118	28-29	0.16531354397352516
2118	30-31	0.1390123512113277
2118	32-33	0.12364844416412524
2118	34-35	0.14362518273473768
2118	36-37	0.2355747570729676
2118	38-39	0.27753937886600255
2118	40-41	0.16492211015387426
2118	42-43	0.2420230763203577
2118	44-45	0.09044019661029523
2118	46-47	0.21434227553958607
2118	48-49	0.29748282839673124
2118	50-51	0.20683394472238348
2118	52-53	0.33567745564648277
2118	54-55	0.20149039226948418
2118	56-57	0.2836043950050424
2118	58-59	0.21140416945598872
2118	60-61	0.2411239982187965
2118	62-63	0.2673495144813103
2118	64-65	0.1454954332796845
2118	66-67	0.18799488752322446
2118	68-69	0.36773822042378157
2118	70-71	0.16481406215762462
2118	72-73	0.24681105205861797
2118	74-75	0.1476256095891486
2118	76	0.2768498404382882
1119	1	0.17175752715728976
1119	2	0.11566616098209437
1119	3	0.17961191206786253
1119	4	-0.004523202226287992
1119	5	0.11140210511243964
1119	6	0.28260558378762113
1119	7	0.483341472138477
1119	8	0.1720017887013654
1119	9	0.11376417087868873
1119	10-11	0.2255681855501379
1119	12-13	-0.018017211168661618
1119	14-15	0.21683393352968494
1119	16-17	0.1552193461866338
1119	18-19	0.2466875525827703
1119	20-21	0.17173221871428979
1119	22-23	0.22235325514085247
1119	24-25	0.12612770488251357
1119	26-27	0.2040371720398113
1119	28-29	0.14164198189074284
1119	30-31	0.2250417237079958
1119	32-33	0.12678076874504995
1119	34-35	0.15617999656058146
1119	36-37	0.19798256006955484
1119	38-39	0.3152155396730052
1119	40-41	0.20210887769578534
1119	42-43	0.3385344247849744
1119	44-45	0.1368815257899456
1119	46-47	0.2526754605269801
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2101	38-39	-0.07885086400877839
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2101	42-43	0.09188550959759567
2101	44-45	-0.23238934863478278
2101	46-47	-0.08308653815809208
2101	48-49	-0.1934349238725659
2101	50-51	-0.06454925391459199
2101	52-53	-0.2441061348344462
2101	54-55	-0.030791361442901177
2101	56-57	-0.8064750534640552
2101	58-59	-0.1093083914075379
2101	60-61	-0.2896652724194979
2101	62-63	-0.09728388231599894
2101	64-65	-0.6279990616813791
2101	66-67	-0.05282013737519975
2101	68-69	0.16170303346839177
2101	70-71	0.21920284615101338
2101	72-73	0.0965528247838563
2101	74-75	0.22462971499727047
2101	76	0.11973015686281485
2107	1	-0.10497178862813428
2107	2	-0.018275551221911712
2107	3	-0.040924889799811126
2107	4	0.04178947896102159
2107	5	0.08312908630744431
2107	6	-0.07158123475569766
2107	7	-0.014572840696644107
2107	8	0.04142491397394821
2107	9	-0.12370790382820473
2107	10-11	-0.01796145861724341
2107	12-13	0.11335275727589078
2107	14-15	0.09029202634889089
2107	16-17	0.015552217732853535
2107	18-19	0.12683396475342334
2107	20-21	-0.08164434302408097
2107	22-23	-0.06151634130851846
2107	24-25	-0.09284335645821074
2107	26-27	0.07299825364785306
2107	28-29	-0.1100427714379748
2107	30-31	0.06275803248964706
2107	32-33	0.022110689984685905
2107	34-35	0.1397978933768158
2107	36-37	-0.0435225837258173
2107	38-39	0.12484315967352444
2107	40-41	0.01718894645075153
2107	42-43	0.20373037645125436
2107	44-45	0.007194797038565071
2107	46-47	-0.08797016917106504
2107	48-49	-0.08160000320294358
2107	50-51	0.05774681174115415
2107	52-53	-0.07094624036236752
2107	54-55	0.11471292238403663
2107	56-57	-0.26882858292484
2107	58-59	0.0779234680780192
2107	60-61	-0.10556594173757361
2107	62-63	0.07246255830268922
2107	64-65	-0.35967033483392186
2107	66-67	0.05876205731588868
2107	68-69	0.010337496176354932
2107	70-71	0.019973156245164603
2107	72-73	-0.018076290483946877
2107	74-75	-0.04347932141149613
2107	76	-0.08426115935342438
2106	1	0.023240999787361716
2106	2	-0.0057036655871911535
2106	3	-0.057728850424922484
2106	4	-0.11467118305102275
2106	5	-0.03083412207929115
2106	6	-0.024796368876586428
2106	7	0.06414527707283924
2106	8	-0.11812827178604834
2106	9	-0.07912813140778496
2106	10-11	0.03385787257169426
2106	12-13	-0.02805937666824576
2106	14-15	-0.18881345748003753
2106	16-17	-0.08533595838380137
2106	18-19	0.03887084701340626
2106	20-21	-0.007011977254890667
2106	22-23	-0.11572623184557074
2106	24-25	-0.04195805673182207
2106	26-27	-0.04601483207634516
2106	28-29	-0.16096173770868916
2106	30-31	-0.1919437442537557
2106	32-33	-0.10511429437771369
2106	34-35	0.03765513648478702
2106	36-37	-0.025529549962321596
2106	38-39	0.07612900000480494
2106	40-41	-0.06568215209615147
2106	42-43	0.1418359081070193
2106	44-45	0.01730712125213074
2106	46-47	-0.28460190312535616
2106	48-49	-0.21842709803781446
2106	50-51	-0.07502702413310658
2106	52-53	-0.06740311690921175
2106	54-55	0.10969128739164802
2106	56-57	-0.06740931511559722
2106	58-59	0.11027691286439278
2106	60-61	0.023320881667245885
2106	62-63	0.08269384980922467
2106	64-65	-0.13618722388667237
2106	66-67	0.13011609443524463
2106	68-69	0.059357314687865426
2106	70-71	0.15828275253505097
2106	72-73	0.027502577712027687
2106	74-75	0.12293086060324043
2106	76	0.055577826421455256
2105	1	0.011051751804856735
2105	2	-0.23597882916494228
2105	3	-0.18821770745616107
2105	4	-0.12093278233845695
2105	5	-0.13504658368550082
2105	6	-0.15402682488299035
2105	7	-0.113927892724341
2105	8	-0.0933473093735131
2105	9	-0.05361979152846175
2105	10-11	-0.16071436712532972
2105	12-13	-0.15807178142786427
2105	14-15	-0.06503828111334542
2105	16-17	-0.07869834367239292
2105	18-19	-0.14439763765521718
2105	20-21	0.037967628749150606
2105	22-23	-0.0713376649743509
2105	24-25	0.020096326983463086
2105	26-27	-0.022306317425098143
2105	28-29	-0.22853537145168445
2105	30-31	-0.2774530466421652
2105	32-33	-0.12863153988008236
2105	34-35	-0.06532129190387082
2105	36-37	-0.12455753391255797
2105	38-39	0.021926923635412265
2105	40-41	-0.1053023698409774
2105	42-43	0.08710786725882969
2105	44-45	-0.119717989141094
2105	46-47	-0.3116898570416069
2105	48-49	-0.15314802638554426
2105	50-51	-0.15455711117084547
2105	52-53	-0.06556163773921497
2105	54-55	-0.08783170522476524
2105	56-57	0.030387129723031592
2105	58-59	-0.02368326863791026
2105	60-61	0.11315680108889836
2105	62-63	-0.13342010346008948
2105	64-65	-0.1409674631333644
2105	66-67	-0.11406922585274515
2105	68-69	-0.285217729331265
2105	70-71	-0.23954970678456533
2105	72-73	-0.33341824867341785
2105	74-75	-0.31164037549572043
2105	76	-0.2632594215092787
2104	1	-0.0077972299353064045
2104	2	-0.11843274861692521
2104	3	-0.0040483419431680545
2104	4	-0.14552212929168462
2104	5	-0.06724904143046473
2104	6	-0.1416084042853356
2104	7	0.0577126576064515
2104	8	0.0897544148959426
2104	9	-0.0793668519024564
2104	10-11	-0.12311924115346073
2104	12-13	-0.20881966644225258
2104	14-15	-0.2203183233757784
2104	16-17	-0.06895798744612591
2104	18-19	-0.2205754411542884
2104	20-21	0.0025954320286629695
2104	22-23	0.002580342996729712
2104	24-25	-0.04599094172277063
2104	26-27	-0.029784171873160403
2104	28-29	-0.15900327598784258
2104	30-31	-0.11435314117709794
2104	32-33	-0.24609906257965264
2104	34-35	-0.13917529450412047
2104	36-37	-0.14217581006793267
2104	38-39	-0.09459744533085512
2104	40-41	-0.3523119122835041
2104	42-43	-0.15442665014578694
2104	44-45	-0.171290667737523
2104	46-47	-0.3781037395129445
2104	48-49	-0.06950023188245069
2104	50-51	-0.17086627707695357
2104	52-53	-0.24645381082444118
2104	54-55	-0.11038615625577819
2104	56-57	-0.037130060327321246
2104	58-59	-0.3241254750304421
2104	60-61	-0.10028860061922273
2104	62-63	-0.26890407852705067
2104	64-65	-0.27772209927188385
2104	66-67	-0.19670268713554862
2104	68-69	0.23360998536705324
2104	70-71	0.23059236216109724
2104	72-73	0.27928614598078383
2104	74-75	0.2372540344840317
2104	76	0.22399122944602112
2111	1	0.019089968031899218
2111	2	-0.1585890822129059
2111	3	-0.02026135879336266
2111	4	-0.212136767598885
2111	5	-0.1546246686492765
2111	6	-0.09079557640872338
2111	7	-0.18473438685304444
2111	8	-0.2212708574387463
2111	9	-0.028536587711222694
2111	10-11	-0.25157059178140884
2111	12-13	-0.2384857519986454
2111	14-15	-0.30665245647477946
2111	16-17	-0.16515771762416165
2111	18-19	-0.38321740056314724
2111	20-21	-0.16096970007196632
2111	22-23	-0.07275473236472152
2111	24-25	-0.1175657355458739
2111	26-27	-0.19862413987451788
2111	28-29	-0.20002691592273436
2111	30-31	-0.07479049405193194
2111	32-33	-0.19689839234375484
2111	34-35	-0.19216092267190277
2111	36-37	-0.07816246447305275
2111	38-39	-0.2218103416299826
2111	40-41	-0.2020303458651327
2111	42-43	-0.275402656874995
2111	44-45	-0.14839781379060213
2111	46-47	-0.16908000578270332
2111	48-49	-0.23369394813528999
2111	50-51	-0.22719098454519582
2111	52-53	-0.12338517076779709
2111	54-55	-0.27301704260278115
2111	56-57	-0.2007817476966096
2111	58-59	-0.24731723150164697
2111	60-61	-0.07117519112126303
2111	62-63	-0.20115184595226765
2111	64-65	-0.022450344788822463
2111	66-67	-0.1676074655174844
2111	68-69	-0.07118972098135856
2111	70-71	-0.08342589366030495
2111	72-73	-0.15823548031028878
2111	74-75	-0.025256245676452238
2111	76	-0.09966735428631779
2110	1	0.009411479123976108
2110	2	-0.04243238413897643
2110	3	-0.0312853541477196
2110	4	-0.0694339514765403
2110	5	0.018834578406611513
2110	6	0.04346849800676722
2110	7	0.039041127627108096
2110	8	0.15441663986749887
2110	9	0.2315366942385424
2110	10-11	0.09251477389623375
2110	12-13	0.14655041467712238
2110	14-15	0.16641479456023944
2110	16-17	0.03293255906491055
2110	18-19	-0.06887467013515192
2110	20-21	0.055727435211174736
2110	22-23	0.14970277307279645
2110	24-25	0.05361948865825639
2110	26-27	0.10362232087367573
2110	28-29	0.08081812881616202
2110	30-31	0.14680274683239247
2110	32-33	0.21298408001973712
2110	34-35	0.15983593132648366
2110	36-37	0.20413426899463616
2110	38-39	0.214431714357616
2110	40-41	0.03709680740554688
2110	42-43	-0.05195089010832277
2110	44-45	0.13403653111870995
2110	46-47	0.17716506322359038
2110	48-49	0.13378892110656437
2110	50-51	0.1696044655278257
2110	52-53	0.10900250569710579
2110	54-55	0.15867744048734522
2110	56-57	0.16779270626467735
2110	58-59	0.17777931797979818
2110	60-61	0.03612781573601609
2110	62-63	0.16482229922188196
2110	64-65	0.2545172425158029
2110	66-67	0.15235269833419096
2110	68-69	0.04728641420852142
2110	70-71	0.1356905901200136
2110	72-73	0.12488555315810856
2110	74-75	0.1610109685692791
2110	76	0.06984465911788362
2109	1	-0.03564961675852629
2109	2	-0.0886274015889228
2109	3	0.005588099015149339
2109	4	0.10084402271319703
2109	5	0.04343777528684001
2109	6	0.12274233592437156
2109	7	-0.18362942850872344
2109	8	0.1665265651452188
2109	9	0.18567781150909468
2109	10-11	0.045684879389369826
2109	12-13	0.1731136618119251
2109	14-15	0.0014106342855768617
2109	16-17	0.10787904679430937
2109	18-19	0.07535871507474212
2109	20-21	-0.10700079842913368
2109	22-23	-0.025860123644783073
2109	24-25	-0.13673564179008935
2109	26-27	-0.023761059272878526
2109	28-29	0.056632347979373776
2109	30-31	-0.004772712337654639
2109	32-33	0.08334837904098436
2109	34-35	0.019606605917829256
2109	36-37	0.21476330212775707
2109	38-39	-0.016436799638292143
2109	40-41	0.1013517016669283
2109	42-43	-0.051914811912816106
2109	44-45	0.09380998782245342
2109	46-47	0.0826910724038683
2109	48-49	0.02871406542182342
2109	50-51	-0.16075166866302482
2109	52-53	-0.016848208234591766
2109	54-55	-0.03500417397496136
2109	56-57	-0.048356573455848206
2109	58-59	-0.07161129526224386
2109	60-61	-0.3401547207213156
2109	62-63	0.012852930626507941
2109	64-65	-0.12142075337341396
2109	66-67	-0.03263578193517702
2109	68-69	0.032869021770885354
2109	70-71	0.20810081789914392
2109	72-73	0.1928551129803182
2109	74-75	0.22320412413638024
2109	76	0.15772653131983816
2108	1	-0.019257334838464146
2108	2	0.017748255815781988
2108	3	0.06279145180966594
2108	4	0.020524653888116973
2108	5	0.036647925938758874
2108	6	-0.20603497066975507
2108	7	-0.16097426595850806
2108	8	0.14281888121668374
2108	9	0.08183133050482638
2108	10-11	-0.10123821804211985
2108	12-13	0.15506061297659102
2108	14-15	0.005008982947643403
2108	16-17	0.1310751921490052
2108	18-19	0.06507838158440649
2108	20-21	0.041683776905472314
2108	22-23	0.0062408262173079265
2108	24-25	0.007090220440012729
2108	26-27	-0.1869982380683055
2108	28-29	0.038060460549502295
2108	30-31	-0.11419804494271801
2108	32-33	0.05188741393625662
2108	34-35	0.017510645461186414
2108	36-37	0.2062816790875459
2108	38-39	-0.052846421915198505
2108	40-41	0.10842407890621786
2108	42-43	-0.030919195538622546
2108	44-45	0.20661535208273563
2108	46-47	-0.056433014959260674
2108	48-49	0.12469061140750881
2108	50-51	0.02587604600470428
2108	52-53	-0.08585591422433225
2108	54-55	-0.004329999729378642
2108	56-57	0.007250027989485375
2108	58-59	-0.17555003973876637
2108	60-61	-0.2519799436746837
2108	62-63	-0.041694655107725964
2108	64-65	-0.20269539948843374
2108	66-67	-0.16956651826458113
2108	68-69	-0.098506984765244
2108	70-71	0.20105532133805326
2108	72-73	0.1766960166711229
2108	74-75	0.26785827747705326
2108	76	0.19167621752637842
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	30.0
13	126.0
14	310.0
15	831.0
16	1224.0
17	1480.0
18	1824.0
19	1797.0
20	1826.0
21	1673.0
22	1852.0
23	2122.0
24	2594.0
25	3366.0
26	4322.0
27	5845.0
28	7921.0
29	10651.0
30	14689.0
31	20550.0
32	30574.0
33	50298.0
34	160360.0
35	230363.0
36	102735.0
37	1039785.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.67236559456199	11.645940617367508	10.259600388429508	30.422093399641
2	21.069215702891793	40.600546625610896	20.594743730990515	17.735493940506792
3	19.703240204525553	19.527211220655953	41.218737346629375	19.55081122818912
4	21.509670706328805	22.431071499194893	14.78272691318964	41.27653088128667
5	45.50028484797122	23.592238952136125	16.321023663568653	14.586452536324002
6	40.571237888075935	26.7171506728062	17.407742214940157	15.303869224177708
7	14.43078665122367	40.380377976779	28.858100982136968	16.330734389860353
8	16.109153271749673	42.04956354699285	22.305950262248466	19.53533291900901
9	40.317581946759326	17.584653992109008	24.101846097347384	17.99591796378429
10-11	29.891381112460802	36.47748994792697	17.43534421876324	16.195784720848987
12-13	28.900916457150387	19.90487019407328	21.887918208612295	29.306295140164035
14-15	17.182777055849037	20.095465267380437	32.98695466915261	29.734803007617916
16-17	29.867663399154402	20.124832857802012	32.091507999284346	17.91599574375924
18-19	29.572427893443315	20.46126538414456	32.34519336704427	17.621113355367854
20-21	29.56751367741085	32.54464579978718	20.3033336629095	17.584506859892464
22-23	41.99833210919329	21.20239978530467	20.057358023296327	16.741910082205713
24-25	17.735287955403635	44.80555594790813	20.502668389879187	16.956487706809042
26-27	29.804072855165398	32.54152659679652	19.88724375453167	17.76715679350641
28-29	30.01197067713777	32.425586410162246	19.911079173611306	17.651363739088673
30-31	29.02968657306704	20.69803054700227	32.33424673013362	17.938036149797078
32-33	17.114654839590575	32.55435652607889	32.7986548584235	17.53233377590704
34-35	29.41929268246749	32.49824029869017	20.13575006826935	17.94671695057299
36-37	17.49343201785361	20.439195551663417	32.273069154495886	29.794303275987083
38-39	17.770334849383694	20.25057205005791	31.86701366328616	30.11207943727224
40-41	41.52212397713683	20.193219912050246	20.090551051347965	18.194105059464956
42-43	29.397163997099728	20.317576061470664	32.53708320385698	17.748176737572624
44-45	29.138829251297118	20.261489260525252	32.529255769937	18.070425718240628
46-47	29.506041837340035	20.23565284330066	20.071217878094487	30.187087441264822
48-49	17.55693428251269	20.345531182613446	20.441019991148526	41.65651454372534
50-51	29.50501191182425	20.676696375603832	32.21892449880882	17.599367213763102
52-53	17.466212557793533	32.61779993785135	32.45727868960517	17.458708814749947
54-55	17.37275417384672	32.61279744248896	20.487925741781783	29.52652264188254
56-57	17.079431386950667	20.64170833451039	44.47803963388796	17.800820644650976
58-59	17.419659924479976	44.5413947663305	20.28720797197661	17.751737337212916
60-61	17.63597370923849	44.97737695038466	19.889833281542792	17.496816058834053
62-63	17.498199101669538	44.44299273990791	20.59727440511502	17.46153375330753
64-65	17.4948150606891	44.580208245054	20.305187528837916	17.619789165418986
66-67	17.35274419239715	44.48471943651892	20.545130747572905	17.617405623511022
68-69	18.605516163356782	38.90264084672825	22.47809495560138	20.013748034313586
70-71	20.03387572153639	35.35510654726593	23.794486896993323	20.816530834204354
72-73	19.596922464853055	34.618091848168966	24.541241748825296	21.24374393815268
74-75	19.78589908377826	33.87681031479232	24.835565034793827	21.501725566635592
76	19.236536813657636	34.320708212096385	25.10999604508602	21.332758929159958
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	233.0
1	127.0
2	23.0
3	24.0
4	23.0
5	27.0
6	35.0
7	32.0
8	25.0
9	40.5
10	54.5
11	56.5
12	60.0
13	59.5
14	74.0
15	137.5
16	210.0
17	234.0
18	331.0
19	542.5
20	846.5
21	1036.0
22	1269.5
23	1906.5
24	2851.5
25	3393.0
26	4061.0
27	5579.5
28	7566.0
29	8702.0
30	9969.5
31	12791.0
32	16620.0
33	18895.0
34	21606.0
35	27622.5
36	35594.5
37	40261.0
38	45972.5
39	93726.0
40	159005.5
41	182134.0
42	182025.0
43	168986.5
44	141681.0
45	115564.5
46	103715.0
47	95554.5
48	81787.0
49	70457.0
50	64734.0
51	59956.0
52	50737.0
53	43356.5
54	40417.0
55	37276.0
56	30496.0
57	22516.5
58	18176.0
59	17584.5
60	17130.0
61	17975.0
62	18683.0
63	18088.5
64	17744.5
65	16649.0
66	12838.0
67	10373.0
68	7296.5
69	4441.0
70	5720.0
71	6778.0
72	5844.0
73	4461.0
74	2578.0
75	1144.0
76	775.0
77	251.0
78	73.5
79	51.0
80	44.0
81	30.5
82	19.0
83	14.0
84	15.0
85	11.0
86	7.0
87	8.0
88	7.5
89	9.5
90	12.0
91	10.0
92	8.0
93	7.0
94	6.5
95	7.0
96	7.0
97	8.5
98	11.0
99	61.0
100	110.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.1770577323276551E-4
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	1699152.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.71528195257095
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.08568026148171	42.45084098991115
2	12.33459316799403	12.75773926906133
3	2.8857038822027343	4.47704969699229
4	0.9013950386733725	1.864635943025682
5	0.5524006999568931	1.4283778974534138
6	0.2770997195971862	0.85981740767681
7	0.1896043870662392	0.6863811035602471
8	0.13945213601375878	0.5769445226271847
9	0.09627852201649423	0.44811638208538285
>10	0.45531809128950307	4.221177654093605
>50	0.044111894348121634	1.620961772146592
>100	0.03465530869616837	3.4163114485777504
>500	0.0024453392584188275	0.8535491309273544
>1k	0.001146868699251342	1.4409886730156882
>5k	0.0	0.0
>10k+	1.1468270607517494E-4	22.897108108845607
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	386068	22.72121623021366	Illumina Single End PCR Primer 1 (100% over 50bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA	4930	0.290144731018767	No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCAT	3454	0.20327787037298606	No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC	3291	0.19368484985451567	No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG	2458	0.14466039530306882	No Hit
CATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	2256	0.13277211220655952	Illumina Single End PCR Primer 1 (98% over 50bp)
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG	2010	0.11829430209892935	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.013536163921768036	0.0	0.0
2	0.014006987014699096	0.0	0.0
3	0.01512519186041037	0.0	0.0
4	0.015949132273039728	0.0	0.0
5	0.01789127753138036	0.0	0.0
6	0.018832923717242482	0.0	0.0
7	0.020363098769268437	0.0	0.0
8	0.0238942719662514	0.0	0.0
9	0.025954122997824798	0.0	0.0
10	0.03389926269103647	0.0	0.0
11	0.03796011186756688	0.0	0.0
12	0.0413147264047007	0.0	0.0
13	0.048671337231748546	0.0	0.0
14	0.05479203743985235	0.0	0.0
15	0.05685188847142575	0.0	0.0
16	0.06744540806237465	0.0	0.0
17	0.06915214177424975	0.0	0.0
18	0.07027034661996101	0.0	0.0
19	0.07798007476670715	0.0	0.0
20	0.07951024981873311	0.0	0.0
21	0.08127583641722459	0.0	0.0
22	0.08209977682985395	0.0	0.0
23	0.0832768345621816	5.885288661638276E-5	0.0
24	0.08421848074804374	5.885288661638276E-5	0.0
25	0.08516012693390586	5.885288661638276E-5	0.0
26	0.08792621260487585	5.885288661638276E-5	0.0
27	0.09063344538922945	5.885288661638276E-5	0.0
28	0.09345838394681583	5.885288661638276E-5	0.0
29	0.09822546776274282	5.885288661638276E-5	0.0
30	0.11870627230524403	5.885288661638276E-5	0.0
31	0.12106038776989934	5.885288661638276E-5	0.0
32	0.12370876766763657	5.885288661638276E-5	0.0
33	0.1260040302456755	5.885288661638276E-5	0.0
34	0.12759305818431782	5.885288661638276E-5	0.0
35	0.129005527463111	5.885288661638276E-5	0.0
36	0.13094767272145164	5.885288661638276E-5	0.0
37	0.13230128911362846	5.885288661638276E-5	0.0
38	0.13389031705227078	5.885288661638276E-5	0.0
39	0.13530278633106396	5.885288661638276E-5	0.0
40	0.13671525560985714	5.885288661638276E-5	0.0
41	0.13818657777526672	5.885288661638276E-5	0.0
42	0.13918707684774523	5.885288661638276E-5	0.0
43	0.14183545674548245	5.885288661638276E-5	0.0
44	0.14324792602427563	5.885288661638276E-5	0.0
45	0.1454843357156982	5.885288661638276E-5	0.0
46	0.14730877520080604	5.885288661638276E-5	0.0
47	0.14960403777884498	5.885288661638276E-5	0.0
48	0.1518404474702675	5.885288661638276E-5	0.0
49	0.15478309180108665	5.885288661638276E-5	0.0
50	0.15719606015235835	5.885288661638276E-5	0.0
51	0.16055067468949216	5.885288661638276E-5	0.0
52	0.1634933190203113	5.885288661638276E-5	0.0
53	0.1669067864440615	5.885288661638276E-5	0.0
54	0.17090878273397553	5.885288661638276E-5	1.1770577323276552E-4
55	0.17620554252944998	5.885288661638276E-5	1.1770577323276552E-4
56	0.1805018032524459	5.885288661638276E-5	1.1770577323276552E-4
57	0.1863870919140842	5.885288661638276E-5	1.1770577323276552E-4
58	0.19197811614264057	5.885288661638276E-5	2.3541154646553104E-4
59	0.19856963944367542	5.885288661638276E-5	2.3541154646553104E-4
60	0.2051611627447103	5.885288661638276E-5	2.3541154646553104E-4
61	0.21410680151040049	5.885288661638276E-5	2.3541154646553104E-4
62	0.22269932295639236	5.885288661638276E-5	2.3541154646553104E-4
63	0.23011478667005658	5.885288661638276E-5	2.3541154646553104E-4
64	0.24047289471453995	5.885288661638276E-5	2.3541154646553104E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGCC	40430	0.0	68.71011	43
TGTAGGG	40930	0.0	68.67457	17
GAGCGTC	40905	0.0	68.65665	9
GCCGTAT	40325	0.0	68.63732	47
TCGTGTA	40890	0.0	68.58767	14
TGGTCGC	40555	0.0	68.58464	42
GTAGGGA	40960	0.0	68.58154	18
GTGTAGG	41060	0.0	68.51681	16
CGTGTAG	41010	0.0	68.50646	15
GTCGTGT	41010	0.0	68.49793	13
GTGGTCG	40780	0.0	68.46371	41
CGTATCA	40450	0.0	68.44252	49
CCGTATC	40395	0.0	68.414406	48
CGCCGTA	40475	0.0	68.4089	46
TAGGGAA	41065	0.0	68.380615	19
AGCGTCG	41110	0.0	68.35685	10
AGAGCGT	41190	0.0	68.30906	8
TCGGAAG	41330	0.0	68.289375	3
TCATTAA	40735	0.0	68.2644	53
CGTCGTG	41120	0.0	68.23808	12
>>END_MODULE