FastQCFastQC Report
Mon 15 Sep 2014
TGA3_4+8_GATCAG_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameTGA3_4+8_GATCAG_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699152
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG38606822.72121623021366Illumina Single End PCR Primer 1 (100% over 50bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA49300.290144731018767No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCAT34540.20327787037298606No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC32910.19368484985451567No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG24580.14466039530306882No Hit
CATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG22560.13277211220655952Illumina Single End PCR Primer 1 (98% over 50bp)
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG20100.11829430209892935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC404300.068.7101143
TGTAGGG409300.068.6745717
GAGCGTC409050.068.656659
GCCGTAT403250.068.6373247
TCGTGTA408900.068.5876714
TGGTCGC405550.068.5846442
GTAGGGA409600.068.5815418
GTGTAGG410600.068.5168116
CGTGTAG410100.068.5064615
GTCGTGT410100.068.4979313
GTGGTCG407800.068.4637141
CGTATCA404500.068.4425249
CCGTATC403950.068.41440648
CGCCGTA404750.068.408946
TAGGGAA410650.068.38061519
AGCGTCG411100.068.3568510
AGAGCGT411900.068.309068
TCGGAAG413300.068.2893753
TCATTAA407350.068.264453
CGTCGTG411200.068.2380812
Produced by FastQC (version 0.11.2)