##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	TGA3_4+8_GATCAG_L001_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1699152
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.77916513649161	34.0	34.0	34.0	34.0	34.0
2	33.79769143666959	34.0	34.0	34.0	34.0	34.0
3	33.737115337533076	34.0	34.0	34.0	34.0	34.0
4	33.781504538734616	34.0	34.0	34.0	34.0	34.0
5	33.83758427733363	34.0	34.0	34.0	34.0	34.0
6	37.55892233302259	38.0	38.0	38.0	37.0	38.0
7	37.51586320705858	38.0	38.0	38.0	37.0	38.0
8	37.49487273651798	38.0	38.0	38.0	37.0	38.0
9	37.525585703927604	38.0	38.0	38.0	37.0	38.0
10-11	37.55655350433628	38.0	38.0	38.0	37.0	38.0
12-13	37.48225408909856	38.0	38.0	38.0	37.0	38.0
14-15	37.470956983248115	38.0	38.0	38.0	37.0	38.0
16-17	37.50164788082526	38.0	38.0	38.0	37.0	38.0
18-19	37.47559517924235	38.0	38.0	38.0	37.0	38.0
20-21	37.49830297701442	38.0	38.0	38.0	37.0	38.0
22-23	37.24154078034219	38.0	38.0	38.0	37.0	38.0
24-25	37.44222088429993	38.0	38.0	38.0	37.0	38.0
26-27	37.43736169571645	38.0	38.0	38.0	37.0	38.0
28-29	37.50519847547483	38.0	38.0	38.0	37.0	38.0
30-31	37.38462274122621	38.0	38.0	38.0	37.0	38.0
32-33	37.359331595996125	38.0	38.0	38.0	37.0	38.0
34-35	37.13305313474015	38.0	38.0	38.0	36.0	38.0
36-37	36.744168561729616	38.0	38.0	38.0	35.5	38.0
38-39	36.86424522349972	38.0	38.0	38.0	36.0	38.0
40-41	36.602856307146155	38.0	37.5	38.0	35.0	38.0
42-43	36.78447778656648	38.0	37.5	38.0	35.0	38.0
44-45	37.27148512905261	38.0	38.0	38.0	37.0	38.0
46-47	37.31233609471077	38.0	38.0	38.0	37.0	38.0
48-49	37.35009463544168	38.0	38.0	38.0	37.0	38.0
50-51	37.23536093298304	38.0	38.0	38.0	37.0	38.0
52-53	37.302910804919165	38.0	38.0	38.0	37.0	38.0
54-55	37.2531177316685	38.0	38.0	38.0	37.0	38.0
56-57	37.21941180071	38.0	38.0	38.0	37.0	38.0
58-59	37.322959629273896	38.0	38.0	38.0	37.0	38.0
60-61	37.169881505598084	38.0	38.0	38.0	37.0	38.0
62-63	37.21893568085727	38.0	38.0	38.0	37.0	38.0
64-65	37.196585120106974	38.0	38.0	38.0	37.0	38.0
66-67	37.25804224695612	38.0	38.0	38.0	37.0	38.0
68-69	37.217738613143496	38.0	38.0	38.0	37.0	38.0
70-71	37.18266964933096	38.0	38.0	38.0	36.5	38.0
72-73	36.96291561908528	38.0	38.0	38.0	36.0	38.0
74-75	33.716995006921096	38.0	32.5	38.0	22.0	38.0
76	32.64597104908802	38.0	29.0	38.0	19.0	38.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.008007738830144717
1101	2	0.04567430343274026
1101	3	-0.04895709794468672
1101	4	0.055130379317979816
1101	5	0.00995981975056992
1101	6	0.04663435704472363
1101	7	-0.038175670114227955
1101	8	-0.015160189982715622
1101	9	0.023205089765568232
1101	10-11	0.043161761696360657
1101	12-13	0.06475798426727408
1101	14-15	0.015180774696112564
1101	16-17	0.08193945863968821
1101	18-19	0.057713370056454494
1101	20-21	0.015396660705000897
1101	22-23	0.2574240804503205
1101	24-25	0.04299876219798904
1101	26-27	0.04397299641440355
1101	28-29	0.04867879404059039
1101	30-31	0.08661676233135296
1101	32-33	0.09437569824453362
1101	34-35	0.11287196524271081
1101	36-37	0.09915027322972492
1101	38-39	0.14430277094305666
1101	40-41	-0.027900327651884993
1101	42-43	0.018693406065359852
1101	44-45	0.0816329304901231
1101	46-47	0.05576618335641825
1101	48-49	0.07192629670628747
1101	50-51	0.10638345569986996
1101	52-53	-0.04271575524560234
1101	54-55	0.06953674604878302
1101	56-57	0.12172513625350234
1101	58-59	0.037078191263304916
1101	60-61	0.08025437541758862
1101	62-63	0.06809085316546515
1101	64-65	0.048304091211832656
1101	66-67	0.0069562266918552496
1101	68-69	0.12475047104165071
1101	70-71	0.08175819512718618
1101	72-73	0.22912312942973045
1101	74-75	0.07095500868165772
1101	76	0.12140729793435412
1102	1	0.003080442314299603
1102	2	0.017426909586056638
1102	3	-0.01085075362552601
1102	4	-0.014743441691777548
1102	5	-0.022002209346865698
1102	6	-0.02646380006219573
1102	7	-0.0022752366377289945
1102	8	-0.17151492606405583
1102	9	-0.03420949256133099
1102	10-11	2.2554020303999778E-4
1102	12-13	-0.020917052361255628
1102	14-15	-0.053062400139893384
1102	16-17	-0.014614356505440185
1102	18-19	-0.0014655829913579055
1102	20-21	-0.13454057897171623
1102	22-23	0.22001482582160747
1102	24-25	-0.10644636437305621
1102	26-27	-0.04660967546735861
1102	28-29	-0.1487425110345626
1102	30-31	-0.12964282204723787
1102	32-33	0.09952197703309196
1102	34-35	0.13180894772520446
1102	36-37	0.10481333487221889
1102	38-39	0.12183061353535862
1102	40-41	0.0769782456704462
1102	42-43	0.20001835342568342
1102	44-45	0.10747795039111452
1102	46-47	0.07774405091596037
1102	48-49	0.0490291666058269
1102	50-51	0.09726517813975732
1102	52-53	-0.06779313481332849
1102	54-55	0.10523423456682224
1102	56-57	0.09052702284105862
1102	58-59	0.06310567055729166
1102	60-61	0.043414846857437794
1102	62-63	0.01899534529928104
1102	64-65	-0.02252805277359471
1102	66-67	0.011841199637125044
1102	68-69	0.042799381284545746
1102	70-71	0.04050875557791045
1102	72-73	0.11124650109937306
1102	74-75	-0.1776556612359954
1102	76	-0.05055647158963694
1103	1	-0.015029004357330678
1103	2	0.0248289520033822
1103	3	-0.08692190202322791
1103	4	-0.010911009174321862
1103	5	0.01770351976347939
1103	6	-0.009909219270909375
1103	7	-0.007339499643194358
1103	8	-0.07265813317830805
1103	9	0.02190679821377728
1103	10-11	0.023213745380644468
1103	12-13	0.062318334858495916
1103	14-15	0.1136721062529844
1103	16-17	0.11233341616257775
1103	18-19	0.11200202401042958
1103	20-21	-0.005998175415378171
1103	22-23	0.28264370573619857
1103	24-25	-0.0444594755204335
1103	26-27	0.041902439834451854
1103	28-29	0.032638224416558614
1103	30-31	0.045070589721625254
1103	32-33	0.08067193780877346
1103	34-35	0.03575620231759302
1103	36-37	0.032508606129546536
1103	38-39	-9.471910036538134E-4
1103	40-41	-0.06641570021767507
1103	42-43	0.04485005749144477
1103	44-45	0.056468145293720795
1103	46-47	-0.002460937945905073
1103	48-49	-0.0035615801898742916
1103	50-51	0.022281349570690168
1103	52-53	-0.17321486508762263
1103	54-55	0.017246756857844048
1103	56-57	-0.04339451966768593
1103	58-59	-0.08008844264863058
1103	60-61	-0.05129495706027143
1103	62-63	0.05048923161362495
1103	64-65	-0.2216074854660377
1103	66-67	0.01634341939949735
1103	68-69	-0.17257161296845425
1103	70-71	-0.0685927862623501
1103	72-73	-0.32713066385708345
1103	74-75	0.11556049721846762
1103	76	-0.18502703172082136
2116	1	-0.030553839555416573
2116	2	0.007407512917545489
2116	3	0.0877319121302449
2116	4	0.024069906967248755
2116	5	0.036801826846804886
2116	6	-0.01401520644360943
2116	7	0.07108129167752253
2116	8	-0.009368281563062908
2116	9	0.014546858025383358
2116	10-11	0.02683793300756321
2116	12-13	0.07339860924714259
2116	14-15	0.11401002533664695
2116	16-17	0.14251730678724783
2116	18-19	0.16393416770567626
2116	20-21	0.0352371892628085
2116	22-23	0.387536392972315
2116	24-25	0.10051818724994632
2116	26-27	0.16076930180036442
2116	28-29	0.07134742636606006
2116	30-31	0.1258583661612036
2116	32-33	0.03193561311941551
2116	34-35	-0.02101434919428158
2116	36-37	-0.11340848575419216
2116	38-39	-0.0945523287119201
2116	40-41	-0.05928044143163902
2116	42-43	0.061174295931074596
2116	44-45	-0.0240258822731505
2116	46-47	-0.027901427920092203
2116	48-49	0.013491248546081636
2116	50-51	0.029991015040735647
2116	52-53	-0.07112526501470029
2116	54-55	0.042693307508614
2116	56-57	0.06477442387396337
2116	58-59	-0.05701634387026644
2116	60-61	-0.10097279573572848
2116	62-63	0.0047139595619754004
2116	64-65	-0.034668267749076165
2116	66-67	0.09319005906948519
2116	68-69	-0.033518916112825536
2116	70-71	-0.030717654624453417
2116	72-73	0.09299538851879419
2116	74-75	-0.052912057128835954
2116	76	-0.4587370288310879
1117	1	0.0010808866599987255
1117	2	0.03164597201663355
1117	3	0.01869823548800298
1117	4	-0.03007336837473673
1117	5	-0.05088467990896817
1117	6	-0.07489748146632991
1117	7	-0.06248407911717635
1117	8	-0.05131941395563189
1117	9	-0.042874233729953914
1117	10-11	-0.007883768839398897
1117	12-13	0.027072283341915693
1117	14-15	0.08301847077768088
1117	16-17	-0.03805359223083116
1117	18-19	0.10962084224748025
1117	20-21	0.025312786946969368
1117	22-23	0.4431874541264307
1117	24-25	0.05629524860184176
1117	26-27	0.09480334386167755
1117	28-29	0.07231947519071724
1117	30-31	-0.014779458350282937
1117	32-33	-0.011564448205739097
1117	34-35	0.06215775910045096
1117	36-37	0.09859249505885259
1117	38-39	0.13504989813878154
1117	40-41	0.18262143607257997
1117	42-43	0.28733228366341024
1117	44-45	0.11147706169970917
1117	46-47	0.06541345771444895
1117	48-49	0.02547884445519344
1117	50-51	0.19724296085470883
1117	52-53	0.14427085096760806
1117	54-55	0.25075745162970264
1117	56-57	0.18206223723785797
1117	58-59	0.23362333329890106
1117	60-61	0.15283497571769544
1117	62-63	0.23028172610354858
1117	64-65	0.21186430448035054
1117	66-67	0.29733331250093187
1117	68-69	0.21331704402616225
1117	70-71	0.29323037299626264
1117	72-73	0.35874705653149874
1117	74-75	0.19259539408503912
1117	76	0.04608871836814643
2117	1	0.014274374635263598
2117	2	0.05671746622560647
2117	3	0.020741045602001407
2117	4	0.017634252544155515
2117	5	0.0052244005236516955
2117	6	0.005702790953868941
2117	7	0.04499642614172217
2117	8	-0.0130102507787484
2117	9	0.0737627578740927
2117	10-11	0.01917530924275468
2117	12-13	0.1375762850865314
2117	14-15	0.10743963637954579
2117	16-17	0.007088849045025825
2117	18-19	0.15252507248487035
2117	20-21	-0.013651508178945448
2117	22-23	0.34955527620245164
2117	24-25	0.11056579505537201
2117	26-27	0.1351299837908826
2117	28-29	0.12149974709986111
2117	30-31	0.18875459680749174
2117	32-33	0.1991289758875041
2117	34-35	0.13028738554772445
2117	36-37	0.2191165606308516
2117	38-39	0.17541452591927964
2117	40-41	0.2590258064602793
2117	42-43	0.33608140077502924
2117	44-45	0.15654525842664668
2117	46-47	-0.08990023806167358
2117	48-49	0.014828348096862953
2117	50-51	0.2243274698283244
2117	52-53	0.12567826038340257
2117	54-55	0.22399969181864776
2117	56-57	-0.0281874019506958
2117	58-59	0.16517978285581592
2117	60-61	0.0821912752347842
2117	62-63	0.22258321251598545
2117	64-65	0.16992900827556667
2117	66-67	0.18635660827034428
2117	68-69	0.08254766958805249
2117	70-71	0.24266388586892873
2117	72-73	0.2973219688668465
2117	74-75	0.12977048579717376
2117	76	-0.15243925483222398
1116	1	-0.015388739264622586
1116	2	-0.01210061402944973
1116	3	-0.04567788118775695
1116	4	0.012405368417304885
1116	5	0.062226723256017635
1116	6	-0.013369648431108772
1116	7	-0.008801292092307733
1116	8	0.03884156343080747
1116	9	0.015975506399030337
1116	10-11	0.05900067041552148
1116	12-13	0.07528888624187857
1116	14-15	0.0525082081923145
1116	16-17	-0.14454400209918816
1116	18-19	-0.03990658047030138
1116	20-21	-0.07046883712272489
1116	22-23	0.3135642606657143
1116	24-25	0.07576497463283971
1116	26-27	-0.011766127293732609
1116	28-29	-0.005676104301556961
1116	30-31	0.140008000575925
1116	32-33	0.09253668851603436
1116	34-35	0.10575005569705098
1116	36-37	0.12011370940064836
1116	38-39	0.05771195918665484
1116	40-41	0.010412722386121231
1116	42-43	0.01645513318155878
1116	44-45	0.014834641483602695
1116	46-47	0.08276747204944712
1116	48-49	0.015892966339258408
1116	50-51	0.1169767372411954
1116	52-53	0.09185391008002597
1116	54-55	0.09258403295930862
1116	56-57	-0.010595108324807256
1116	58-59	0.08579672981136355
1116	60-61	0.05742565933544341
1116	62-63	0.10074100664731844
1116	64-65	0.05885699809964251
1116	66-67	0.0828428598994222
1116	68-69	0.08562161550458569
1116	70-71	0.17039757221171925
1116	72-73	0.22237603044310816
1116	74-75	0.07001817615998362
1116	76	-0.2935233555689152
2118	1	0.06704857400674058
2118	2	0.062314823036928146
2118	3	0.07728888443344317
2118	4	0.03407886916888003
2118	5	0.0660533352500039
2118	6	-0.03631069459100189
2118	7	0.08487292912121092
2118	8	0.09912863462616173
2118	9	0.07603669018379833
2118	10-11	0.12848606520925188
2118	12-13	0.07025635251034146
2118	14-15	-0.017927597974086495
2118	16-17	-0.09585867673611403
2118	18-19	6.142277614173963E-5
2118	20-21	-0.01113723004475986
2118	22-23	0.23176057148836549
2118	24-25	0.024579036005263788
2118	26-27	0.05947301574491348
2118	28-29	-0.051289608564289324
2118	30-31	0.18249930490135569
2118	32-33	0.004997291968301454
2118	34-35	0.08861813031598587
2118	36-37	0.03182972652211902
2118	38-39	-0.08722953993694205
2118	40-41	-0.150289872663933
2118	42-43	0.030113742404658694
2118	44-45	-0.09143111862158548
2118	46-47	0.04220175882579724
2118	48-49	-0.22615094221041687
2118	50-51	0.018130288765917157
2118	52-53	-0.04917716093144264
2118	54-55	-0.008702197697104452
2118	56-57	-0.06164338442738426
2118	58-59	-0.08071577860492596
2118	60-61	-0.2048036175751733
2118	62-63	-0.0942195442960454
2118	64-65	-0.2649111922766849
2118	66-67	-0.012881240028406182
2118	68-69	-0.2502950574037044
2118	70-71	0.11294419913804887
2118	72-73	-0.055411340944722554
2118	74-75	-0.010448616211043316
2118	76	-0.5745106499965118
1119	1	-0.03541248518329354
1119	2	0.038275363016161634
1119	3	-0.0011333081195914474
1119	4	0.013206001641805187
1119	5	0.02017942930132932
1119	6	0.06254407473105772
1119	7	0.056951552470863476
1119	8	0.10255720605473329
1119	9	0.1198999152245932
1119	10-11	0.12768277192932231
1119	12-13	0.022296702606773522
1119	14-15	-0.11297046995154147
1119	16-17	0.05627097761784228
1119	18-19	0.025307825387031357
1119	20-21	0.0577633781736111
1119	22-23	0.2550080125208609
1119	24-25	0.11373865623964718
1119	26-27	0.10225494720908301
1119	28-29	0.16100203955913628
1119	30-31	0.1985243753656718
1119	32-33	0.20484597910670743
1119	34-35	0.13498039110815085
1119	36-37	0.20255973690633766
1119	38-39	0.1121634284653723
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2101	26-27	-0.07622828750491095
2101	28-29	-0.06395391047373522
2101	30-31	0.023767636378174473
2101	32-33	0.0249445984360932
2101	34-35	0.15167467118705247
2101	36-37	0.16742429969397676
2101	38-39	0.15954804384998766
2101	40-41	0.06410768041801873
2101	42-43	0.07480567879321853
2101	44-45	0.10608588630015703
2101	46-47	0.05569269875095273
2101	48-49	0.133881342782324
2101	50-51	0.14303068192655388
2101	52-53	0.09906101594081207
2101	54-55	0.04828173617396203
2101	56-57	0.14239002764341535
2101	58-59	0.04148025924172316
2101	60-61	-0.005369026285798384
2101	62-63	-0.06111992165651969
2101	64-65	0.05569771953999236
2101	66-67	0.02197375400702839
2101	68-69	-0.005414598777740309
2101	70-71	0.03083395615792739
2101	72-73	0.380306368137461
2101	74-75	0.35237832200770214
2101	76	0.42814440881958404
2107	1	0.017144276149736015
2107	2	-0.022271341619671148
2107	3	0.028365550240039283
2107	4	-0.002200345365558576
2107	5	-0.02386007915193744
2107	6	-0.07332960083783746
2107	7	-0.24783199764586783
2107	8	-0.19502426088900648
2107	9	-0.11971007990098315
2107	10-11	-6.328595645612722E-4
2107	12-13	-0.30248653965188055
2107	14-15	0.05680289519379045
2107	16-17	-0.10021107682172925
2107	18-19	-0.03617871864950217
2107	20-21	0.026167621484113113
2107	22-23	0.23705195926140732
2107	24-25	0.06904215299431371
2107	26-27	0.0674370452618902
2107	28-29	-0.005569755510002494
2107	30-31	0.04350564733262985
2107	32-33	-0.10392720645474895
2107	34-35	-0.04432723607570921
2107	36-37	-0.2058545117916779
2107	38-39	-0.15464054290382734
2107	40-41	-0.06234282980209116
2107	42-43	-0.17928120549631643
2107	44-45	-0.026161609734344893
2107	46-47	-0.1609207348821755
2107	48-49	-0.04529413495557577
2107	50-51	-0.21109227094247274
2107	52-53	-0.042552123951999477
2107	54-55	-0.18269516217412018
2107	56-57	0.007087113056272187
2107	58-59	-0.0174171625379671
2107	60-61	0.060634807367002
2107	62-63	-0.1960625876437163
2107	64-65	0.06402830446577923
2107	66-67	-0.25366823255195925
2107	68-69	-0.06929249024137363
2107	70-71	-0.2171608630824764
2107	72-73	0.13655381498754338
2107	74-75	-0.06430875164188166
2107	76	0.16085912958183002
2106	1	0.04415908322270212
2106	2	0.05391550055600902
2106	3	0.031143679055070095
2106	4	-0.018094553035304273
2106	5	-0.03634924574823373
2106	6	-0.018417198265453294
2106	7	-0.4304386038928669
2106	8	-0.1437263569299816
2106	9	-0.024850379682916923
2106	10-11	0.030439717942421396
2106	12-13	0.10686257365061635
2106	14-15	0.060019314954210756
2106	16-17	-0.043682288953455384
2106	18-19	-0.06289657689191586
2106	20-21	-0.12342295317694152
2106	22-23	0.1465409543174161
2106	24-25	0.057572579388008194
2106	26-27	0.027914686686536072
2106	28-29	0.015123616749612268
2106	30-31	-0.018579632866632778
2106	32-33	-0.036485998906997
2106	34-35	-0.01578747275751624
2106	36-37	-0.19052168266547653
2106	38-39	-0.2417728715120404
2106	40-41	0.003843379724187912
2106	42-43	-0.1103594523346274
2106	44-45	-0.06170803026614635
2106	46-47	-0.15168582705157263
2106	48-49	-0.050128478606858096
2106	50-51	-0.2522873169587143
2106	52-53	-0.07726895493452446
2106	54-55	-0.3843505912734031
2106	56-57	-0.0035122155843367864
2106	58-59	0.011532988392985999
2106	60-61	0.08360524803676839
2106	62-63	-0.07223995833302155
2106	64-65	-0.03303072599928214
2106	66-67	-0.25853201813086457
2106	68-69	0.06750769222034592
2106	70-71	-0.10030439203624297
2106	72-73	0.08486406478913722
2106	74-75	0.06890621766680027
2106	76	0.2604859707519438
2105	1	0.008430170569319273
2105	2	-0.01775768230746877
2105	3	-0.03949195598983124
2105	4	-0.034168865722151054
2105	5	-0.0665248302105681
2105	6	-0.07235966939505545
2105	7	-0.03965205522960957
2105	8	0.011848702189340088
2105	9	-0.12636262835582102
2105	10-11	-0.04140090246813344
2105	12-13	0.09028700122735245
2105	14-15	-0.0246154600985804
2105	16-17	-0.010100403312883088
2105	18-19	-0.10083466362313231
2105	20-21	-0.08568106627082983
2105	22-23	0.2779598087918913
2105	24-25	0.08313004626541698
2105	26-27	0.034393646617907336
2105	28-29	0.018287775499715053
2105	30-31	-0.015614627211256504
2105	32-33	-0.06062468937896881
2105	34-35	-0.13775357887819695
2105	36-37	-0.33619227158236953
2105	38-39	-0.14288977753735566
2105	40-41	-0.12572172025092243
2105	42-43	-0.4058832303666762
2105	44-45	-0.18952158455130075
2105	46-47	-0.24472029155393926
2105	48-49	-0.24581001773388067
2105	50-51	-0.42494023546193205
2105	52-53	-0.22867797784603994
2105	54-55	-0.7237017635878971
2105	56-57	-0.3962670542469553
2105	58-59	-0.20514483392865657
2105	60-61	-0.07285096401435709
2105	62-63	-0.6921926793112902
2105	64-65	-0.7706860589930216
2105	66-67	-0.8342437843576391
2105	68-69	-0.2564320865545966
2105	70-71	-1.0135072586492342
2105	72-73	-0.5191671687849109
2105	74-75	-0.7263585971405959
2105	76	-0.4809780271451274
2104	1	-0.03991806194802194
2104	2	0.024223662539576196
2104	3	-0.026577627804194037
2104	4	-0.07288856631488017
2104	5	-0.04957150015945189
2104	6	-0.18754600187148185
2104	7	-0.033717759104355594
2104	8	0.05026377091737544
2104	9	0.03734498346823045
2104	10-11	-0.004880668447952985
2104	12-13	0.09376317963848635
2104	14-15	-0.05893329152002735
2104	16-17	-0.06217057239837942
2104	18-19	-0.06354593113167084
2104	20-21	0.016965089878574702
2104	22-23	0.2748157077076243
2104	24-25	0.1549424639098831
2104	26-27	0.07527635599716831
2104	28-29	0.10015102775685847
2104	30-31	-0.1317504705559216
2104	32-33	-0.07564432705393642
2104	34-35	-0.07669503369908881
2104	36-37	-0.15563516291150847
2104	38-39	-0.1453178201310763
2104	40-41	0.09784827415377606
2104	42-43	-0.15004944845959756
2104	44-45	-0.09826049543571003
2104	46-47	-0.021062497200979635
2104	48-49	-0.15802712395176144
2104	50-51	-0.10692606553575956
2104	52-53	-0.2057037261318797
2104	54-55	-0.11025309906111147
2104	56-57	-0.27344855999845663
2104	58-59	-0.05394262566932184
2104	60-61	-0.14335174387397132
2104	62-63	-0.13489160938249967
2104	64-65	-0.5469081428730931
2104	66-67	-0.09752092878775898
2104	68-69	-0.46055269462961235
2104	70-71	-0.1696964222718762
2104	72-73	-0.6460630630152551
2104	74-75	0.1091033807075732
2104	76	-0.08495394784974764
2111	1	0.022012771398593145
2111	2	-0.025513124266304033
2111	3	-0.03877543217134871
2111	4	-0.025971151414864835
2111	5	-0.01630606824440406
2111	6	-0.12749608591150974
2111	7	-0.06164817979151849
2111	8	-0.003695359764094519
2111	9	-0.03033747879592852
2111	10-11	-0.06590714328709879
2111	12-13	0.026667965435862584
2111	14-15	-0.11935868949638717
2111	16-17	-0.11586467610817408
2111	18-19	-0.051303508838770995
2111	20-21	-0.026444118479666656
2111	22-23	0.22144665598359836
2111	24-25	0.05984530505713792
2111	26-27	0.04741648223809136
2111	28-29	0.0023416201035288964
2111	30-31	-0.15824715520103183
2111	32-33	-0.03820622972862964
2111	34-35	-0.0816764585125469
2111	36-37	-0.0819743996398472
2111	38-39	0.03145927853231001
2111	40-41	-0.2939185335766865
2111	42-43	-0.1442786796794593
2111	44-45	-0.09476115736431012
2111	46-47	0.05309580228936994
2111	48-49	-0.1691300981160495
2111	50-51	0.015254224353988377
2111	52-53	-0.14008090347914504
2111	54-55	0.10739920539337788
2111	56-57	-0.062010823624909506
2111	58-59	0.07286412474977055
2111	60-61	-0.14710538658564332
2111	62-63	0.0352040989492437
2111	64-65	-0.29587945041531327
2111	66-67	0.058951857083371806
2111	68-69	-0.11907547502434568
2111	70-71	0.0629627870634053
2111	72-73	-0.34558866498647944
2111	74-75	0.2217559772704334
2111	76	0.0013861727193642537
2110	1	-0.003818314612992424
2110	2	0.006435325342977194
2110	3	0.056986205103200405
2110	4	-0.004057537159098956
2110	5	0.007601344189673398
2110	6	0.02409115381996685
2110	7	-0.013122901639725626
2110	8	0.050399573516997975
2110	9	0.03357146453433302
2110	10-11	-0.009358272014466706
2110	12-13	-0.03646151981372725
2110	14-15	-0.14520017724080958
2110	16-17	-0.0946473558394203
2110	18-19	-0.17642742134755451
2110	20-21	-0.0972618529464242
2110	22-23	0.19100481525712354
2110	24-25	-0.12719437471680806
2110	26-27	-0.08731134024723275
2110	28-29	-0.023311193147137033
2110	30-31	-0.2677104128465757
2110	32-33	-0.045081153022074716
2110	34-35	-0.027819222246002084
2110	36-37	-0.0698557338845518
2110	38-39	0.024453412850824918
2110	40-41	-0.2105579268003055
2110	42-43	-0.06233542098279088
2110	44-45	0.035136329328196325
2110	46-47	0.13041646123566153
2110	48-49	0.033928648382577364
2110	50-51	0.14769762225471084
2110	52-53	0.008220483594421069
2110	54-55	0.14959082362729248
2110	56-57	-0.00973646732046518
2110	58-59	0.05214347478182901
2110	60-61	0.036806133786370765
2110	62-63	0.14690143977858838
2110	64-65	0.0845795307742776
2110	66-67	0.19472741774921332
2110	68-69	0.10932067332845463
2110	70-71	0.18180278382642712
2110	72-73	0.025787587787768018
2110	74-75	0.29459545524080255
2110	76	-0.03757732496234922
2109	1	0.021609895133885004
2109	2	-0.0778478522399979
2109	3	0.016535976994781265
2109	4	-0.039128171570979475
2109	5	0.02634197067774835
2109	6	7.941611892405831E-4
2109	7	0.03142010639606241
2109	8	0.1203867599305326
2109	9	0.051267831829505894
2109	10-11	-0.022412826114255324
2109	12-13	0.013961936537896236
2109	14-15	-0.052341844883606825
2109	16-17	-0.0438602411954534
2109	18-19	-0.02482240533279878
2109	20-21	-0.16953314867782865
2109	22-23	0.04691745375399847
2109	24-25	-0.11156024203613413
2109	26-27	-0.21998175058252656
2109	28-29	-0.21931800391697465
2109	30-31	-0.6768288680511461
2109	32-33	-0.1233828633417886
2109	34-35	-0.0752131764033308
2109	36-37	-0.1513946625798468
2109	38-39	-0.12372452173472226
2109	40-41	-0.4120177770502522
2109	42-43	-0.09534002699054867
2109	44-45	-0.12671159634169982
2109	46-47	-0.00869694450928904
2109	48-49	-0.027178615489724223
2109	50-51	0.0660323578850992
2109	52-53	-0.03706580965688744
2109	54-55	-0.040150729537288044
2109	56-57	0.01191870705648057
2109	58-59	0.10460027839553732
2109	60-61	-0.08561015909696579
2109	62-63	0.031608470449377535
2109	64-65	0.154667860115282
2109	66-67	0.1051744927969196
2109	68-69	-0.021610172879050538
2109	70-71	0.08011603024213798
2109	72-73	-0.44606090620668937
2109	74-75	0.24846341965793073
2109	76	-0.14551773161890225
2108	1	0.029951743530375552
2108	2	-0.05868512701483297
2108	3	-0.08506165816961442
2108	4	0.005206099257542007
2108	5	0.017421589537562454
2108	6	-0.07057596821986323
2108	7	-0.10420114294363714
2108	8	0.05627358353478229
2108	9	0.009146224129594316
2108	10-11	0.011428653765470642
2108	12-13	0.0646268390247613
2108	14-15	-0.08853607992787005
2108	16-17	-0.011356764774234307
2108	18-19	0.0228090577856932
2108	20-21	0.015442734885908749
2108	22-23	0.13975879954772807
2108	24-25	0.0343849944293666
2108	26-27	-0.0214222619570279
2108	28-29	-0.03293421736548652
2108	30-31	-0.030834167231390097
2108	32-33	-0.004654716405411818
2108	34-35	-0.12760632827100693
2108	36-37	-0.26783284917853933
2108	38-39	-0.20375223153902056
2108	40-41	-0.5084808852848113
2108	42-43	-0.09926096820589692
2108	44-45	-0.2639988617754341
2108	46-47	-0.3049923817668585
2108	48-49	-0.20588002140046768
2108	50-51	-0.12733166450735922
2108	52-53	-0.11717438122888524
2108	54-55	-0.14368870531919242
2108	56-57	-0.2126671620370999
2108	58-59	-0.09960190649333356
2108	60-61	-0.44004420631535623
2108	62-63	-0.14665803489742046
2108	64-65	-0.03793293168548928
2108	66-67	-0.11249322725377908
2108	68-69	-0.4899528640739135
2108	70-71	-0.13889552840065988
2108	72-73	-1.4204927668369365
2108	74-75	-0.11828444407279193
2108	76	-0.38644790758016967
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	5.0
16	8.0
17	13.0
18	29.0
19	64.0
20	164.0
21	245.0
22	349.0
23	517.0
24	826.0
25	1169.0
26	1826.0
27	2809.0
28	3866.0
29	5963.0
30	9163.0
31	14157.0
32	21553.0
33	32515.0
34	52548.0
35	106285.0
36	265813.0
37	1179265.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.046020603218544	12.063135022646591	10.008168780662354	25.88267559347251
2	20.57714671789222	39.89607757281279	22.026869873913576	17.499905835381412
3	19.80546766857821	18.40571061329416	41.56249705735567	20.22632466077196
4	21.61389916852642	22.455436594254074	15.066986355546765	40.863677881672736
5	45.33743891070369	23.289499703381452	16.511648163319116	14.861413222595743
6	40.561821426217314	26.816965168507583	17.73737723287852	14.883836172396583
7	14.215267380434476	40.39503234554648	29.358232812602992	16.031467461416046
8	16.196961778581315	42.17315460888726	22.301359737092387	19.328523875439043
9	40.28727271015189	17.462651958153245	24.068299951976044	18.18177537971882
10-11	29.824553659707902	36.30811134024501	17.612138290158853	16.255196709888224
12-13	16.915849788600433	31.931751838564175	21.78598500899272	29.366413363842668
14-15	17.089907200768383	32.23081278190533	20.94435930393514	29.73492071339115
16-17	29.79289080670829	20.406502773148016	20.0439689915911	29.756637428552597
18-19	17.64591984707666	20.68758415962786	32.229753429946236	29.43674256334925
20-21	29.553182999519763	20.486424993173067	32.467048268783486	17.493343738523688
22-23	17.53624749286703	44.89742530391631	20.205932135559383	17.36039506765728
24-25	17.700358767196814	20.653714323380132	32.58119344237596	29.064733467047095
26-27	17.747676488036383	20.487572624462086	19.924939028409465	41.839811859092066
28-29	29.976894356714407	32.431471698823884	19.945243274292118	17.64639067016959
30-31	17.031995960337863	20.763739794909462	32.24131802216635	29.96294622258632
32-33	17.123217934593256	32.563125606184734	20.691762714577624	29.62189374464439
34-35	29.43329966948219	32.35472753467612	20.227795982937373	17.98417681290432
36-37	17.69323756791623	20.785191672081133	31.839499938792997	29.68207082120964
38-39	29.574428891588273	32.21889507236551	20.10338098063034	18.103295055415877
40-41	17.61772931438741	32.28542826068533	31.802422620224675	18.29441980470258
42-43	17.206995018691675	20.436929715528688	32.63469071631025	29.72138454946938
44-45	29.188383381828114	20.24242092526154	20.62431730651525	29.944878386395096
46-47	17.51688489317024	32.37411956081622	32.08576984283925	18.02322570317429
48-49	29.62183489175777	20.430014501351263	32.405105605619745	17.543045001271224
50-51	17.562966703390867	20.515939715811182	20.252219930883168	41.66887364991478
52-53	29.524345085077737	20.61472428599678	32.38900934113016	17.471921287795325
54-55	17.483456453572135	20.506582106839176	32.599143572793956	29.41081786679473
56-57	17.183218452498657	20.461589075020953	32.54335103628163	29.811841436198762
58-59	29.56718998653446	20.35374116029643	32.349430774880645	17.729638078288463
60-61	17.724517877152838	20.763357251146456	32.105632692072284	29.406492179628422
62-63	29.644669811764928	20.29727181558801	32.67709422111736	17.380964151529703
64-65	17.69291387703984	44.642680584197294	20.259458835936986	17.404946702825878
66-67	17.454765671346646	44.624377336459595	20.48910279951411	17.431754192679644
68-69	17.47195071423863	44.52494538452122	20.149521643737582	17.853582257502566
70-71	17.956810220627702	44.79558038362666	19.91360984773581	17.33399954800983
72-73	17.757622625874554	44.43322316072959	20.230385509948494	17.578768703447366
74-75	17.70433133704342	41.175715886512805	22.02951825381131	19.090434522632467
76	18.249632757987513	39.956813751800894	22.399290940422045	19.39426254978954
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	271.0
1	210.5
2	136.0
3	106.5
4	91.0
5	80.0
6	53.5
7	38.5
8	39.0
9	41.5
10	55.0
11	62.5
12	59.0
13	72.5
14	104.0
15	145.0
16	235.5
17	303.0
18	368.0
19	559.0
20	850.0
21	1015.0
22	1260.0
23	1895.5
24	2823.0
25	3360.0
26	4021.5
27	5538.5
28	7330.5
29	8267.0
30	9568.0
31	12741.0
32	16543.5
33	18474.0
34	21376.0
35	27560.0
36	35251.5
37	39661.0
38	44918.5
39	58409.0
40	70553.0
41	84615.5
42	94767.0
43	236400.5
44	275847.5
45	140905.0
46	108149.0
47	97063.0
48	80497.5
49	69573.5
50	64129.0
51	59865.0
52	50807.0
53	43074.5
54	40136.0
55	37326.5
56	30628.0
57	22433.0
58	18127.0
59	17510.5
60	16758.5
61	17621.0
62	18619.0
63	18090.0
64	17521.5
65	16368.5
66	12721.5
67	10188.0
68	7068.0
69	4328.0
70	5557.5
71	6407.0
72	5618.0
73	4368.5
74	2485.5
75	1063.0
76	712.5
77	224.5
78	60.0
79	33.0
80	23.5
81	12.5
82	7.5
83	4.0
84	4.5
85	3.0
86	0.5
87	0.0
88	1.0
89	2.0
90	1.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	1699152.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.39241782331968
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.01091129839433	42.14739019518291
2	12.338950970254492	12.682570475295494
3	2.7985264304501505	4.314691188098926
4	1.0752521930532337	2.210392398833303
5	0.5177931758478264	1.330532161961757
6	0.3006185692662462	0.9269709070307695
7	0.16802892967388655	0.6044789072143903
8	0.12707699780454249	0.52246353335233
9	0.09915648193294826	0.45863022144496735
>10	0.4696502753358007	4.2956561339952755
>50	0.05120930115289557	1.8762429877907043
>100	0.037497766246195004	3.677068990217701
>500	0.0029121294595829813	1.0878639149106122
>1k	0.002185463989206397	2.589956861693931
>5k	1.1500856929448642E-4	0.5145149832629903
>10k+	1.1500856929448642E-4	20.760576139713844
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC	351245	20.67178215957136	TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGCACTCCAGTCACGATCAGATCTCGTATGC	8705	0.5123143779956119	TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGACCTCGTATGC	4660	0.2742544516323437	TruSeq Adapter, Index 9 (98% over 50bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA	4634	0.2727242765803177	No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC	3782	0.2225816171831596	No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCAT	3490	0.20539657429117583	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAAGATCTCGTATGC	2869	0.16884893170240214	TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCAGATCTCGTATGC	2762	0.16255167283444918	TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAACTCGTATGC	2681	0.1577845890185222	TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACCAGATCTCGTATGC	2452	0.14430727798337054	TruSeq Adapter, Index 9 (98% over 50bp)
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG	2324	0.13677410849647353	No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG	1984	0.1167641270469034	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.008357109899526352	0.0	0.0
2	0.00876908010584103	0.0	0.0
3	0.009769579178319538	0.0	0.0
4	0.010534666704332514	0.0	0.0
5	0.011888283096509317	0.0	0.0
6	0.012712223509138676	0.0	0.0
7	0.013948134128082714	0.0	0.0
8	0.017067337118751	0.0	0.0
9	0.01853865928416057	0.0	0.0
10	0.024659359492264377	0.0	0.0
11	0.0273077393900016	0.0	0.0
12	0.028661355782178405	0.0	0.0
13	0.03372270403118732	0.0	0.0
14	0.039549139806209214	0.0	0.0
15	0.040961609085002404	0.0	0.0
16	0.051202011356253005	0.0	0.0
17	0.05249677486181342	0.0	0.0
18	0.053438421047675544	0.0	0.0
19	0.06103044342118892	0.0	0.0
20	0.06220750115351658	0.0	0.0
21	0.06356111754569338	0.0	0.0
22	0.0642084992984736	0.0	0.0
23	0.06526785125756848	0.0	0.0
24	0.06615064455681423	0.0	0.0
25	0.06720999651590912	0.0	0.0
26	0.0679162311553057	0.0	0.0
27	0.06874017156793506	0.0	0.0
28	0.07091772837274123	0.0	0.0
29	0.07403693136340951	0.0	0.0
30	0.09440003013267795	0.0	0.0
31	0.0955770878650056	0.0	1.1770577323276552E-4
32	0.09793120332966092	0.0	1.1770577323276552E-4
33	0.09957908415491963	0.0	1.1770577323276552E-4
34	0.10110925920694558	0.0	1.1770577323276552E-4
35	0.10252172848573878	0.0	1.1770577323276552E-4
36	0.10416960931099749	0.0	1.1770577323276552E-4
37	0.10540551992994153	0.0	1.1770577323276552E-4
38	0.10675913632211832	0.0	1.1770577323276552E-4
39	0.10805389982767875	0.0	1.1770577323276552E-4
40	0.10893669312692449	5.885288661638276E-5	1.1770577323276552E-4
41	0.11040801529233406	5.885288661638276E-5	1.1770577323276552E-4
42	0.11134966147819618	5.885288661638276E-5	1.1770577323276552E-4
43	0.11358607116961873	5.885288661638276E-5	1.1770577323276552E-4
44	0.11493968756179553	5.885288661638276E-5	1.1770577323276552E-4
45	0.11717609725321808	5.885288661638276E-5	1.1770577323276552E-4
46	0.11894168385170956	5.885288661638276E-5	1.1770577323276552E-4
47	0.12141350508959764	5.885288661638276E-5	1.1770577323276552E-4
48	0.12388532632748571	5.885288661638276E-5	1.1770577323276552E-4
49	0.12676911777168848	5.885288661638276E-5	1.1770577323276552E-4
50	0.12882896880326186	5.885288661638276E-5	1.1770577323276552E-4
51	0.13236014200024482	5.885288661638276E-5	1.1770577323276552E-4
52	0.1348319632381329	5.885288661638276E-5	1.1770577323276552E-4
53	0.13801001911541758	5.885288661638276E-5	1.1770577323276552E-4
54	0.14195316251871523	5.885288661638276E-5	1.7655865984914828E-4
55	0.14601401169524564	5.885288661638276E-5	1.7655865984914828E-4
56	0.14978059643869412	5.885288661638276E-5	1.7655865984914828E-4
57	0.1553716206672505	5.885288661638276E-5	1.7655865984914828E-4
58	0.16025641025641027	5.885288661638276E-5	1.7655865984914828E-4
59	0.16614169891804853	5.885288661638276E-5	1.7655865984914828E-4
60	0.1723801048993851	5.885288661638276E-5	1.7655865984914828E-4
61	0.18038409747921316	5.885288661638276E-5	1.7655865984914828E-4
62	0.18856464871889037	5.885288661638276E-5	1.7655865984914828E-4
63	0.19539158356639078	5.885288661638276E-5	1.7655865984914828E-4
64	0.2051023098580939	5.885288661638276E-5	1.7655865984914828E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCACACG	41440	0.0	68.46284	11
CACACGT	41485	0.0	68.32951	12
ACGTCTG	41470	0.0	68.32892	15
AGCACAC	41540	0.0	68.31488	10
TATGCCG	41150	0.0	68.30741	46
GTATGCC	41155	0.0	68.29061	45
ACACGTC	41510	0.0	68.2715	13
CACGTCT	41540	0.0	68.247475	14
CGTATGC	41245	0.0	68.217964	44
ATGCCGT	41210	0.0	68.17399	47
GCCGTCT	41180	0.0	68.17265	49
AGTCACG	41080	0.0	68.168205	28
GAGCACA	41615	0.0	68.16653	9
CTCGTAT	41060	0.0	68.09912	42
TCGGAAG	41625	0.0	68.09129	3
GTCACGA	40670	0.0	68.080894	29
TGCCGTC	41255	0.0	68.06569	48
TCGTATG	41330	0.0	68.06073	43
TCTCGTA	40035	0.0	67.998	41
AGAGCAC	41720	0.0	67.97819	8
>>END_MODULE