FastQCFastQC Report
Mon 15 Sep 2014
TGA3_4+8_GATCAG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameTGA3_4+8_GATCAG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699152
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC35124520.67178215957136TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGCACTCCAGTCACGATCAGATCTCGTATGC87050.5123143779956119TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGACCTCGTATGC46600.2742544516323437TruSeq Adapter, Index 9 (98% over 50bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA46340.2727242765803177No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC37820.2225816171831596No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCAT34900.20539657429117583No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAAGATCTCGTATGC28690.16884893170240214TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCAGATCTCGTATGC27620.16255167283444918TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAACTCGTATGC26810.1577845890185222TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACCAGATCTCGTATGC24520.14430727798337054TruSeq Adapter, Index 9 (98% over 50bp)
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG23240.13677410849647353No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG19840.1167641270469034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG414400.068.4628411
CACACGT414850.068.3295112
ACGTCTG414700.068.3289215
AGCACAC415400.068.3148810
TATGCCG411500.068.3074146
GTATGCC411550.068.2906145
ACACGTC415100.068.271513
CACGTCT415400.068.24747514
CGTATGC412450.068.21796444
ATGCCGT412100.068.1739947
GCCGTCT411800.068.1726549
AGTCACG410800.068.16820528
GAGCACA416150.068.166539
CTCGTAT410600.068.0991242
TCGGAAG416250.068.091293
GTCACGA406700.068.08089429
TGCCGTC412550.068.0656948
TCGTATG413300.068.0607343
TCTCGTA400350.067.99841
AGAGCAC417200.067.978198
Produced by FastQC (version 0.11.2)