##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	TGA3_3+7_ACTTGA_L001_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2549930
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.36584886643947	34.0	34.0	34.0	34.0	34.0
2	33.454195605369556	34.0	34.0	34.0	34.0	34.0
3	33.42648857027448	34.0	34.0	34.0	34.0	34.0
4	33.46408097477185	34.0	34.0	34.0	34.0	34.0
5	33.55495680273576	34.0	34.0	34.0	34.0	34.0
6	37.156568611687376	38.0	38.0	38.0	36.0	38.0
7	37.01621926876424	38.0	38.0	38.0	36.0	38.0
8	37.02620189573832	38.0	38.0	38.0	36.0	38.0
9	37.123656335664116	38.0	38.0	38.0	36.0	38.0
10-11	37.23760848337014	38.0	38.0	38.0	37.0	38.0
12-13	37.14273234951548	38.0	38.0	38.0	37.0	38.0
14-15	37.069851133168356	38.0	38.0	38.0	36.0	38.0
16-17	37.15028236069226	38.0	38.0	38.0	36.5	38.0
18-19	37.201516708301796	38.0	38.0	38.0	37.0	38.0
20-21	37.22016055342696	38.0	38.0	38.0	37.0	38.0
22-23	37.28767181844208	38.0	38.0	38.0	37.0	38.0
24-25	37.214361570709784	38.0	38.0	38.0	37.0	38.0
26-27	37.22690348362504	38.0	38.0	38.0	37.0	38.0
28-29	37.17909079857094	38.0	38.0	38.0	37.0	38.0
30-31	37.16535885298812	38.0	38.0	38.0	37.0	38.0
32-33	37.15088061240897	38.0	38.0	38.0	37.0	38.0
34-35	37.184721345291834	38.0	38.0	38.0	37.0	38.0
36-37	37.02467597149726	38.0	38.0	38.0	36.0	38.0
38-39	37.02732702466342	38.0	38.0	38.0	36.0	38.0
40-41	37.17785311753656	38.0	38.0	38.0	37.0	38.0
42-43	37.06065166494767	38.0	38.0	38.0	36.0	38.0
44-45	37.01163110359892	38.0	38.0	38.0	36.0	38.0
46-47	36.9626860345186	38.0	38.0	38.0	36.0	38.0
48-49	36.85348774280078	38.0	38.0	38.0	36.0	38.0
50-51	36.93445408305326	38.0	38.0	38.0	36.0	38.0
52-53	36.88769083857204	38.0	38.0	38.0	36.0	38.0
54-55	36.993511586592575	38.0	38.0	38.0	36.0	38.0
56-57	36.89841564278235	38.0	38.0	38.0	36.0	38.0
58-59	37.03874616165933	38.0	38.0	38.0	36.0	38.0
60-61	37.02915001588279	38.0	38.0	38.0	36.0	38.0
62-63	37.052336730812215	38.0	38.0	38.0	36.5	38.0
64-65	36.92498578392348	38.0	38.0	38.0	36.0	38.0
66-67	36.878355876435826	38.0	38.0	38.0	36.0	38.0
68-69	34.307312553677946	38.0	37.0	38.0	21.0	38.0
70-71	33.881579690422875	38.0	37.0	38.0	19.0	38.0
72-73	33.96939014012149	38.0	37.0	38.0	16.5	38.0
74-75	34.0028620393501	38.0	37.0	38.0	15.0	38.0
76	33.64865819846035	38.0	36.0	38.0	11.0	38.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.08983118733902273
1101	2	0.1438932893429623
1101	3	0.1325112580635306
1101	4	0.09426249221784389
1101	5	0.073909185537957
1101	6	0.1681835218890626
1101	7	0.1610861403109567
1101	8	0.2952429731666726
1101	9	0.13029524730787756
1101	10-11	0.13367708409979429
1101	12-13	0.13782048879699715
1101	14-15	0.23879076191205684
1101	16-17	-0.004095820892629831
1101	18-19	-0.052865613318353155
1101	20-21	0.011722368319340148
1101	22-23	0.048014877422957625
1101	24-25	-0.00428205780924884
1101	26-27	0.029490943019602867
1101	28-29	0.14231156408973078
1101	30-31	0.016584783746132814
1101	32-33	0.09013888259614333
1101	34-35	0.08538038343422016
1101	36-37	0.08921414664471428
1101	38-39	0.13970350805987408
1101	40-41	0.10746549532611738
1101	42-43	0.0489654248198903
1101	44-45	0.06279928405874102
1101	46-47	0.15869787435540417
1101	48-49	0.12301967685277759
1101	50-51	0.09074981181183261
1101	52-53	-0.01966268210959754
1101	54-55	0.09907816648316725
1101	56-57	0.17111971392311176
1101	58-59	0.01914994254613589
1101	60-61	0.18881367904689483
1101	62-63	0.1291799042162438
1101	64-65	0.27128814163771864
1101	66-67	0.06090269864590425
1101	68-69	0.17953443708534422
1101	70-71	0.11091200065649787
1101	72-73	0.09969072412749824
1101	74-75	0.15601590812077148
1101	76	-0.03729237667555907
1102	1	0.08210533080526972
1102	2	0.10773061411575924
1102	3	0.1184732449499677
1102	4	0.03830163212213478
1102	5	0.11776100608396689
1102	6	0.19541543154918628
1102	7	0.09028635697954002
1102	8	0.3300314025728994
1102	9	0.21648165974773548
1102	10-11	0.2057621220195287
1102	12-13	0.2759706824305539
1102	14-15	0.22533511418347985
1102	16-17	0.10430985141798033
1102	18-19	0.07525878701118671
1102	20-21	0.17193577173094354
1102	22-23	0.14082289148337424
1102	24-25	0.12126269394076417
1102	26-27	0.07658954466901946
1102	28-29	0.1972224571059371
1102	30-31	0.05472074712093189
1102	32-33	0.18413736696935956
1102	34-35	0.1343370926172085
1102	36-37	0.20011388849223977
1102	38-39	0.15771173045040143
1102	40-41	0.11185923309064805
1102	42-43	0.10341656088716178
1102	44-45	0.15976607752502048
1102	46-47	0.1531935290795161
1102	48-49	0.2323439194974526
1102	50-51	0.09644797167589303
1102	52-53	0.142304916128829
1102	54-55	0.19651538136369595
1102	56-57	0.3016123825969359
1102	58-59	0.14204885361864683
1102	60-61	0.29539695661670606
1102	62-63	0.2104469809381868
1102	64-65	0.4193613349791008
1102	66-67	0.28094473989917645
1102	68-69	0.1652652508400081
1102	70-71	-0.04144900992666578
1102	72-73	0.021338710973779484
1102	74-75	-0.04302231510031618
1102	76	0.09197777343118219
1103	1	0.022355612779364264
1103	2	0.1701662231883958
1103	3	0.0793274791001295
1103	4	0.08140025198213863
1103	5	0.054210024977869864
1103	6	0.05081214569526793
1103	7	-0.054171809228478196
1103	8	0.28206985485481795
1103	9	0.12957988648823004
1103	10-11	0.035521985874275686
1103	12-13	0.15559986816825244
1103	14-15	-0.09609001092470493
1103	16-17	0.048073468353351245
1103	18-19	-0.009972613560897514
1103	20-21	0.07594296691838309
1103	22-23	-0.0021779041323881643
1103	24-25	0.0011057071925080209
1103	26-27	-9.415671297219319E-4
1103	28-29	0.17833711509703676
1103	30-31	0.05880703284555722
1103	32-33	0.07946991591262531
1103	34-35	0.1157832938611989
1103	36-37	0.14754665746357176
1103	38-39	-0.03279952696455268
1103	40-41	0.12247414198706963
1103	42-43	0.16153765041016044
1103	44-45	0.13795780617624587
1103	46-47	0.10814952897015928
1103	48-49	0.13906563125861737
1103	50-51	0.0760817401392444
1103	52-53	0.10732048308882014
1103	54-55	0.023027970278370447
1103	56-57	0.22503929598064332
1103	58-59	0.18248549756846444
1103	60-61	0.2809608975477076
1103	62-63	0.05437307993059903
1103	64-65	0.3545796653182549
1103	66-67	0.12146031810686253
1103	68-69	0.1597769085941252
1103	70-71	0.013906486101873838
1103	72-73	0.0559121624472283
1103	74-75	0.05123441368144199
1103	76	0.0909135993798813
2116	1	-0.054511019092096547
2116	2	0.07916646865545829
2116	3	-0.02216776886061922
2116	4	0.005752019059478641
2116	5	0.017025557066645547
2116	6	0.005799889560869076
2116	7	-0.26014234768801714
2116	8	0.1896892772412997
2116	9	0.08663683794246424
2116	10-11	-0.05638077882768755
2116	12-13	-0.009950361450911771
2116	14-15	-0.06645740419742907
2116	16-17	0.03324677372498286
2116	18-19	-0.13696063632870192
2116	20-21	-0.005127184918535477
2116	22-23	-0.055030012601193334
2116	24-25	-0.14220328170158325
2116	26-27	-0.19241413892055448
2116	28-29	-0.008961797310803377
2116	30-31	-0.06384018756359922
2116	32-33	-0.1764380003283108
2116	34-35	-0.04798145819287214
2116	36-37	0.051501009394989694
2116	38-39	-0.1421155666178393
2116	40-41	0.05595091073725911
2116	42-43	-0.15718934631576786
2116	44-45	-0.3469736284774072
2116	46-47	-0.12671586366532495
2116	48-49	0.017225463927758256
2116	50-51	-0.07843145150322073
2116	52-53	-0.013916631252492095
2116	54-55	-0.2498988105708051
2116	56-57	0.051162007019875944
2116	58-59	-0.09486633825360968
2116	60-61	0.06796783666755601
2116	62-63	-0.24448985088513098
2116	64-65	0.06426355247384663
2116	66-67	-0.19530043554982512
2116	68-69	0.14404104641383242
2116	70-71	-0.10138827424646024
2116	72-73	0.1039304202099629
2116	74-75	0.09769685881153123
2116	76	-0.019896602791085627
1117	1	-0.1519595372542284
1117	2	-0.013560988372944394
1117	3	0.00180307535374169
1117	4	0.08057849561981101
1117	5	-0.09496761317803504
1117	6	-0.3106775415564087
1117	7	-1.2094966118610273
1117	8	-0.13304234265803672
1117	9	-0.15959066709546477
1117	10-11	-0.15158163592595741
1117	12-13	-0.12864530908240113
1117	14-15	-0.49638911891504023
1117	16-17	-0.15902701626727378
1117	18-19	-0.3121268958894632
1117	20-21	-0.00491441790744318
1117	22-23	-0.15101242143666127
1117	24-25	-0.06522601770829795
1117	26-27	-0.17282823436034533
1117	28-29	-0.03261562744349078
1117	30-31	-0.1553637799191847
1117	32-33	-0.08134495102557082
1117	34-35	-0.14788621920862255
1117	36-37	0.06742854844496549
1117	38-39	-0.3742741433831469
1117	40-41	0.00739163921644348
1117	42-43	-0.36243062826965655
1117	44-45	-0.10967539175278063
1117	46-47	-0.309489749041191
1117	48-49	0.024053542967706676
1117	50-51	-0.21467534989972137
1117	52-53	0.021320467233032048
1117	54-55	-0.36519730866359623
1117	56-57	0.09197041297775854
1117	58-59	-0.3492635048894428
1117	60-61	-0.07970583544607734
1117	62-63	-0.6464619534706486
1117	64-65	-0.03397398946984964
1117	66-67	-0.4408382884225972
1117	68-69	0.08939956364820034
1117	70-71	-0.4715311112489218
1117	72-73	-0.21404747849076244
1117	74-75	-0.3818191231901835
1117	76	-0.008826714284069226
2117	1	0.027692011015197693
2117	2	0.043987774583456485
2117	3	-0.00655751804781346
2117	4	0.06688539378556158
2117	5	0.008035882843380193
2117	6	-0.35459155751991034
2117	7	0.061180190135594614
2117	8	0.1755428005242834
2117	9	-0.022025736906122972
2117	10-11	0.06798943184031003
2117	12-13	0.02857563051396994
2117	14-15	-0.06372088001936049
2117	16-17	0.1387815377006376
2117	18-19	-0.06689961058830107
2117	20-21	0.11968906336061025
2117	22-23	0.04185243683001261
2117	24-25	0.07853082658131427
2117	26-27	-0.07515004572271522
2117	28-29	0.1490822262048539
2117	30-31	0.12960828967105442
2117	32-33	0.10459153221047757
2117	34-35	0.08602545202310097
2117	36-37	0.20171471812802366
2117	38-39	-0.06674287809738644
2117	40-41	0.0942335884876897
2117	42-43	-0.09135054558053923
2117	44-45	0.12478900389040604
2117	46-47	-0.010561058745125251
2117	48-49	0.19212534357241395
2117	50-51	0.035888176085087764
2117	52-53	0.20052787763925295
2117	54-55	-0.1862360731392414
2117	56-57	0.2565423720933566
2117	58-59	0.14582531390143316
2117	60-61	0.18113468503168662
2117	62-63	-0.23907866095788677
2117	64-65	0.1622920690887284
2117	66-67	-0.10691816474019333
2117	68-69	0.13742302940765683
2117	70-71	-0.0954972118721571
2117	72-73	-0.07892921443531975
2117	74-75	-0.13286999333155336
2117	76	0.052105356114090284
1116	1	0.023980089854390485
1116	2	0.010558573627648116
1116	3	0.05477732809123381
1116	4	-0.027145683131301723
1116	5	0.04161125630997731
1116	6	0.036762529470522054
1116	7	0.2936108163721798
1116	8	0.32552528089922816
1116	9	0.14449338143960233
1116	10-11	0.1242396129642529
1116	12-13	0.14310668855003428
1116	14-15	0.03631286392012356
1116	16-17	0.1818282663607178
1116	18-19	0.13297520532390905
1116	20-21	0.18774613255127548
1116	22-23	0.12207133115285984
1116	24-25	0.1325395934192528
1116	26-27	0.11194536729695415
1116	28-29	0.16596383393561354
1116	30-31	0.1112831570444115
1116	32-33	0.14601521470393664
1116	34-35	0.05417060233621385
1116	36-37	0.2891657488001016
1116	38-39	0.21806086697495175
1116	40-41	0.2710051343333433
1116	42-43	0.0904400435267334
1116	44-45	0.18405056267160802
1116	46-47	0.14493463687180252
1116	48-49	0.3518132351843377
1116	50-51	0.10775732248855974
1116	52-53	0.3044663683872244
1116	54-55	0.1418313980543715
1116	56-57	0.31888666038823743
1116	58-59	0.1442996794846323
1116	60-61	0.19722520924170084
1116	62-63	0.18373928950065022
1116	64-65	0.36769810286515536
1116	66-67	0.11664913389169129
1116	68-69	0.3089171724081936
1116	70-71	-0.07015496445993108
1116	72-73	0.09425379407508672
1116	74-75	-4.932871440104236E-4
1116	76	0.08853937035635795
2118	1	0.04770685219873627
2118	2	-0.1072833802466846
2118	3	0.015009240509563426
2118	4	0.039591000347627414
2118	5	-0.017007232067399514
2118	6	0.07835859067251505
2118	7	0.3091086119236124
2118	8	0.32177497420913426
2118	9	-0.003988696260940117
2118	10-11	0.11145990350207313
2118	12-13	0.1336236076457311
2118	14-15	0.12180622335198166
2118	16-17	0.14629094838014112
2118	18-19	0.19585621551971855
2118	20-21	0.17855932543783126
2118	22-23	0.13913319147478376
2118	24-25	0.13700142550337802
2118	26-27	0.08498171695526224
2118	28-29	0.16479823651556558
2118	30-31	0.18195549613201223
2118	32-33	0.1856220246551672
2118	34-35	0.14934455853295248
2118	36-37	0.21097290469512586
2118	38-39	0.21695426609379354
2118	40-41	0.19583638803867842
2118	42-43	0.1997114152636641
2118	44-45	0.14718352485193265
2118	46-47	0.2221402955677263
2118	48-49	0.2782664998155866
2118	50-51	0.15979314511695009
2118	52-53	0.27440607812599893
2118	54-55	0.1768037847856121
2118	56-57	0.3149907224049784
2118	58-59	0.2360285310417254
2118	60-61	0.25839054931779515
2118	62-63	0.2575436533013118
2118	64-65	0.16322824410060122
2118	66-67	0.1701948827752915
2118	68-69	0.19682412051110987
2118	70-71	0.05380759584630823
2118	72-73	0.09473042746297722
2118	74-75	0.06249659092309656
2118	76	0.20241315050006392
1119	1	0.1180617990353312
1119	2	0.12952350833818116
1119	3	0.1887479509214529
1119	4	-0.00784526923112594
1119	5	0.1261181860079077
1119	6	0.31292376420232415
1119	7	0.4602452334024676
1119	8	0.19619696811592746
1119	9	0.16014631124592427
1119	10-11	0.20521528389970456
1119	12-13	-0.05068729361104829
1119	14-15	0.2239517835411462
1119	16-17	0.18401346853318756
1119	18-19	0.224141149987382
1119	20-21	0.20623504341445908
1119	22-23	0.23993593534501656
1119	24-25	0.09066071126282793
1119	26-27	0.16199568939374132
1119	28-29	0.15082979138976071
1119	30-31	0.21475227248252793
1119	32-33	0.15632965062373927
1119	34-35	0.19000723891006288
1119	36-37	0.18279271409009823
1119	38-39	0.3008159994816495
1119	40-41	0.15221989800290459
1119	42-43	0.29143116415595927
1119	44-45	0.16991808203874115
1119	46-47	0.27455425535838884
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2101	30-31	-0.021540294055867548
2101	32-33	-0.20017971004147483
2101	34-35	0.048602471496273836
2101	36-37	-0.47997332004007376
2101	38-39	-0.08925451055058886
2101	40-41	-0.016490122536900742
2101	42-43	0.007603225215412124
2101	44-45	-0.2403605259242667
2101	46-47	-0.15407957384841353
2101	48-49	-0.14830275652477098
2101	50-51	-0.08152666218554572
2101	52-53	-0.29143194406101003
2101	54-55	-0.017025650682469973
2101	56-57	-0.7268764257297136
2101	58-59	-0.11226880799819128
2101	60-61	-0.24342929392201285
2101	62-63	-0.0873516358598252
2101	64-65	-0.5518050994716504
2101	66-67	-0.04115796571136343
2101	68-69	0.03935439470633639
2101	70-71	0.019473059210568522
2101	72-73	-0.021299514656817564
2101	74-75	0.06360347840476521
2101	76	-0.09195586848861836
2107	1	-0.09864496508577503
2107	2	-0.06860829569022542
2107	3	-0.0649273060498956
2107	4	-0.0039611583015783935
2107	5	0.08168370105170197
2107	6	-0.06777642092961855
2107	7	-0.011762407932110364
2107	8	-0.020280853052291548
2107	9	-0.09884344676714818
2107	10-11	0.021581033980261566
2107	12-13	0.03289535130510046
2107	14-15	7.539028271779102E-4
2107	16-17	-0.027876791328921513
2107	18-19	0.15497242587657212
2107	20-21	-0.09391575603357438
2107	22-23	-0.08859848837779083
2107	24-25	-0.10414503308005862
2107	26-27	0.06074649619730366
2107	28-29	-0.14802014498315685
2107	30-31	6.255683742679707E-4
2107	32-33	0.015896873276822987
2107	34-35	0.14033118177928117
2107	36-37	-0.08238966668931624
2107	38-39	0.1706540786050823
2107	40-41	0.045192221811007016
2107	42-43	0.1683028186560378
2107	44-45	0.037475948410332194
2107	46-47	-0.10565191499646431
2107	48-49	-0.15177492509144486
2107	50-51	0.028757561901144868
2107	52-53	-0.11585031578072602
2107	54-55	0.11149895375413621
2107	56-57	-0.24635053305731702
2107	58-59	0.10102269096476846
2107	60-61	-0.11137867168302051
2107	62-63	0.07486468749715414
2107	64-65	-0.31831311740142354
2107	66-67	0.03999777928127202
2107	68-69	-0.007764864343258182
2107	70-71	0.10043922474170586
2107	72-73	0.009462815263461266
2107	74-75	0.09585738033074165
2107	76	-0.010776473175489798
2106	1	0.07627532484601574
2106	2	0.010957102345322767
2106	3	0.030317143635734567
2106	4	-0.029372268021255366
2106	5	-0.09614021499907466
2106	6	-0.006324184356195417
2106	7	0.06608392514917938
2106	8	-0.1417011106728978
2106	9	-0.097451144717013
2106	10-11	0.056188328861694004
2106	12-13	-0.06654850370856025
2106	14-15	-0.17188607491643637
2106	16-17	-0.06604269853044542
2106	18-19	0.09941264633747693
2106	20-21	0.08595563081755841
2106	22-23	-0.0800825724686689
2106	24-25	-0.05825223285340542
2106	26-27	-0.006980911616317087
2106	28-29	-0.08360262632938031
2106	30-31	-0.14334676046677686
2106	32-33	-0.09802536226337821
2106	34-35	0.0556171230517819
2106	36-37	-0.056583748885344676
2106	38-39	0.09802938022185259
2106	40-41	-0.0366340469326758
2106	42-43	0.21172226101231928
2106	44-45	0.04319489251708575
2106	46-47	-0.2397873569423865
2106	48-49	-0.0960410393401645
2106	50-51	0.004703796077336619
2106	52-53	0.03458060051721645
2106	54-55	0.18987874096760038
2106	56-57	-0.02991474233307656
2106	58-59	0.14622486227688825
2106	60-61	0.028298230998956342
2106	62-63	0.10113008140709923
2106	64-65	-0.06170169070346532
2106	66-67	0.15011149277190583
2106	68-69	0.09217826248200822
2106	70-71	0.08488074677775614
2106	72-73	-0.02331173220642313
2106	74-75	0.11055121444253047
2106	76	0.028347725570831983
2105	1	0.03754091291332173
2105	2	-0.21491452635061137
2105	3	-0.121364773979181
2105	4	-0.05576567074890448
2105	5	-0.09110322497961221
2105	6	-0.191551513837517
2105	7	-0.11551060030977567
2105	8	-0.09672111988067655
2105	9	-0.08006983105599375
2105	10-11	-0.10979890102308332
2105	12-13	-0.1415860748894886
2105	14-15	-0.055451100982431
2105	16-17	-0.06436977075701122
2105	18-19	-0.07606619893100941
2105	20-21	0.05130240260180585
2105	22-23	-0.09443147184873624
2105	24-25	-0.061648803819124964
2105	26-27	-0.05004520351192099
2105	28-29	-0.14260077660138393
2105	30-31	-0.24442776620301743
2105	32-33	-0.12257904841620615
2105	34-35	-0.05761240485441732
2105	36-37	-0.06849935243514693
2105	38-39	-0.011282915066288979
2105	40-41	-0.10451311631155136
2105	42-43	0.0821328788323683
2105	44-45	-0.07591367389378689
2105	46-47	-0.23735333466028408
2105	48-49	-0.12181620885316136
2105	50-51	-0.08315058756006266
2105	52-53	-0.024784634948524342
2105	54-55	-0.04285411181784582
2105	56-57	0.09822582246506073
2105	58-59	0.003853855151305652
2105	60-61	0.06785855056511281
2105	62-63	-0.16001387138079792
2105	64-65	-0.10485002399593668
2105	66-67	-0.021502197394575262
2105	68-69	-0.07266006112141099
2105	70-71	-0.0031134334349687265
2105	72-73	-0.003655080586398185
2105	74-75	0.04219121124614844
2105	76	0.08273785563295633
2104	1	0.01909936683286162
2104	2	-0.06564159039110962
2104	3	-0.024008186090036077
2104	4	-0.15382616734384413
2104	5	-0.019530635418526288
2104	6	-0.11354369422599575
2104	7	0.010196333293116311
2104	8	0.046057479875941
2104	9	-0.06891647988530991
2104	10-11	-0.10097100543332971
2104	12-13	-0.1762913197130942
2104	14-15	-0.20213920345045722
2104	16-17	-0.10002802091409535
2104	18-19	-0.22196430378527765
2104	20-21	0.009491421967211977
2104	22-23	-0.03161670577149067
2104	24-25	0.002901052209246302
2104	26-27	-0.030121906820816946
2104	28-29	-0.17873444629879032
2104	30-31	-0.06748481138477302
2104	32-33	-0.18975010404579962
2104	34-35	-0.16243846822079178
2104	36-37	-0.06727269212581177
2104	38-39	-0.1365927060576837
2104	40-41	-0.3293873557940614
2104	42-43	-0.1070717741440319
2104	44-45	-0.19550735220681048
2104	46-47	-0.28991511383404145
2104	48-49	-0.12905825023106132
2104	50-51	-0.1509412588447816
2104	52-53	-0.16785318708574692
2104	54-55	-0.13149230651436028
2104	56-57	-0.05777173472008457
2104	58-59	-0.2727843090156483
2104	60-61	-0.01734917512307277
2104	62-63	-0.24867398676013863
2104	64-65	-0.23686052886304765
2104	66-67	-0.19251062961790666
2104	68-69	-0.09060450268499665
2104	70-71	-0.0410084574960905
2104	72-73	0.009598458318720304
2104	74-75	-0.10919862514452916
2104	76	-0.015924298044389218
2111	1	0.04587159459462953
2111	2	-0.07969114938637034
2111	3	0.015442672938341673
2111	4	-0.1065772560413123
2111	5	-0.13339626578628838
2111	6	-0.042295055042394836
2111	7	-0.05399870023481412
2111	8	-0.10361132530729833
2111	9	0.017394425573584726
2111	10-11	-0.18255589554180318
2111	12-13	-0.15852727742716155
2111	14-15	-0.1455956744439817
2111	16-17	-0.13156737456244372
2111	18-19	-0.3639803959658323
2111	20-21	-0.0936686943911269
2111	22-23	-0.010419944802784187
2111	24-25	-0.057227308898603724
2111	26-27	-0.12870207884113682
2111	28-29	-0.21277717438155008
2111	30-31	-0.05963357484902332
2111	32-33	-0.15396159730996573
2111	34-35	-0.060960857098613985
2111	36-37	-0.03975854557788239
2111	38-39	-0.12285054477702317
2111	40-41	-0.14480278907073085
2111	42-43	-0.1165056776262432
2111	44-45	-0.0522215675472566
2111	46-47	-0.15281471312383843
2111	48-49	-0.16770929424355074
2111	50-51	-0.17322624054061464
2111	52-53	-0.10899782237807898
2111	54-55	-0.23913667545299688
2111	56-57	-0.10889793058433384
2111	58-59	-0.20173886098530147
2111	60-61	0.061550247928025215
2111	62-63	-0.14622923586160397
2111	64-65	-0.030329366278373016
2111	66-67	-0.0618189934079183
2111	68-69	0.031122475547789463
2111	70-71	0.00203654670011133
2111	72-73	-0.0551524100581986
2111	74-75	-0.052755189428367544
2111	76	-0.20566008812325975
2110	1	-0.019768693727151287
2110	2	-0.04175457529574089
2110	3	0.017498277853448485
2110	4	-0.03156938709222601
2110	5	0.02116053145988417
2110	6	0.056513355011375666
2110	7	0.0799787461800392
2110	8	0.15110365743332466
2110	9	0.22833546905290802
2110	10-11	0.08820953086804906
2110	12-13	0.0910001169785204
2110	14-15	0.1270835087198705
2110	16-17	-0.013283566200342989
2110	18-19	-0.12635152830106477
2110	20-21	0.010466263971011358
2110	22-23	0.10805037509069848
2110	24-25	-0.0020010079498860023
2110	26-27	0.0580949035803755
2110	28-29	-0.016786578320648005
2110	30-31	0.07303332098352655
2110	32-33	0.09456079497261527
2110	34-35	0.08438656681235557
2110	36-37	0.0698723723731689
2110	38-39	0.13497095083772592
2110	40-41	0.025873027229202705
2110	42-43	-0.162037564090177
2110	44-45	0.006171702038145099
2110	46-47	0.1284196089215186
2110	48-49	0.06939286607724426
2110	50-51	0.16297677790979037
2110	52-53	0.01825160717761065
2110	54-55	0.08200010649994738
2110	56-57	0.06720364599558337
2110	58-59	0.07384715774497863
2110	60-61	-0.01862328092725818
2110	62-63	0.09778513140077649
2110	64-65	0.12992783064938607
2110	66-67	0.12374054772553933
2110	68-69	0.1320906287555772
2110	70-71	0.19453348195152387
2110	72-73	0.14482696937162132
2110	74-75	0.1571947806216727
2110	76	0.19249389129802097
2109	1	-0.040588205043597725
2109	2	-0.13528258224157952
2109	3	-0.020630497117501534
2109	4	0.11308497005067863
2109	5	0.025065135128777172
2109	6	0.134769468850493
2109	7	-0.022718925731119555
2109	8	0.21622108873255996
2109	9	0.20561706318048323
2109	10-11	0.1303542486550242
2109	12-13	0.2014817419545949
2109	14-15	0.027250640449516084
2109	16-17	0.10237327005430075
2109	18-19	0.05331489922906485
2109	20-21	0.003752991529609062
2109	22-23	-0.030430633343115687
2109	24-25	-0.05175841824988936
2109	26-27	-0.01912585994582372
2109	28-29	0.051037552677257736
2109	30-31	0.012723240014750559
2109	32-33	0.13031559341816745
2109	34-35	0.052502582011122456
2109	36-37	0.23014977574616324
2109	38-39	0.018557663004159508
2109	40-41	0.03964543050218339
2109	42-43	-0.023288781210283105
2109	44-45	0.14923598127574422
2109	46-47	0.12726896178210723
2109	48-49	0.08113532212534835
2109	50-51	-0.1312701361432147
2109	52-53	0.0022225351346847333
2109	54-55	0.009434322648928628
2109	56-57	-0.03147124808034363
2109	58-59	-0.03829609356135677
2109	60-61	-0.23854382142217645
2109	62-63	0.04813005385325653
2109	64-65	-0.06954199006667494
2109	66-67	0.020735428306842607
2109	68-69	-0.1612659892811905
2109	70-71	-7.178590332515E-4
2109	72-73	-0.009432322590981812
2109	74-75	-0.023561645285809618
2109	76	-0.029819504922478757
2108	1	-0.05117501804704716
2108	2	-0.027636247663785696
2108	3	1.913256981822542E-5
2108	4	-0.0025784108353050783
2108	5	-0.021019210707692082
2108	6	-0.19880963241833172
2108	7	-0.17053360471150114
2108	8	0.12317344573477129
2108	9	0.09923727885348654
2108	10-11	-0.1782449285191845
2108	12-13	0.11836496391718754
2108	14-15	-0.011851738104674325
2108	16-17	0.16524370419494971
2108	18-19	-0.001362884633159922
2108	20-21	-0.004289384513413097
2108	22-23	-0.11806647740957743
2108	24-25	-0.016947688527643834
2108	26-27	-0.20953654735375693
2108	28-29	-0.016817536174436043
2108	30-31	-0.09864636446857844
2108	32-33	0.043958101798203586
2108	34-35	-0.003927071091325729
2108	36-37	0.22427644216062959
2108	38-39	-0.04429769819775231
2108	40-41	0.060462630198699685
2108	42-43	-4.98159127026554E-4
2108	44-45	0.1260499274191531
2108	46-47	-0.0466414833262192
2108	48-49	0.02704739401021783
2108	50-51	-0.10731185879974703
2108	52-53	-0.18910481769970744
2108	54-55	-0.10469355552820048
2108	56-57	-0.0240192887856594
2108	58-59	-0.23338586995325272
2108	60-61	-0.26795850258729104
2108	62-63	-0.021244744993396125
2108	64-65	-0.08957902757963865
2108	66-67	-0.2043521423975747
2108	68-69	-0.1555576112274224
2108	70-71	-0.01059618040663679
2108	72-73	-0.00865896651670539
2108	74-75	-0.1472756902141299
2108	76	-0.0856621390387673
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	9.0
12	41.0
13	221.0
14	574.0
15	1316.0
16	1782.0
17	2263.0
18	2752.0
19	2769.0
20	2696.0
21	2647.0
22	2802.0
23	3087.0
24	3690.0
25	4679.0
26	6017.0
27	8211.0
28	11202.0
29	14979.0
30	19650.0
31	27360.0
32	39777.0
33	63216.0
34	179845.0
35	263836.0
36	170094.0
37	1714414.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.497070500924735	10.995504179732416	11.894550582684033	34.61287473665882
2	24.68777574286353	33.352405752314766	22.19507986493747	19.764738639884232
3	23.32150294321805	21.464510790492287	34.131133011494434	21.082853254795232
4	24.555576035420582	25.394461808755537	15.061472275709528	34.98848988011435
5	40.71268623060241	26.62382888942049	17.439419905644467	15.224064974332629
6	34.077327612914864	29.901291407999437	19.15166298682709	16.86971799225861
7	15.899377629974158	32.758350229221975	32.840979948469176	18.50129219233469
8	18.56470569780347	34.935547250316674	24.226743479232763	22.27300357264709
9	34.848956638025356	18.971265877886843	26.294721815892984	19.885055668194813
10-11	28.502703995795965	34.633715435325676	18.648904087563185	18.214676481315173
12-13	27.068037161804444	21.63861360900103	23.82392849999804	27.469420729196486
14-15	19.09734777033095	21.883502684387416	30.98706631162424	28.032083233657396
16-17	28.347229139623444	21.868168930127492	30.024530085139595	19.760071845109472
18-19	27.76538963814693	22.142882353633237	30.613369778778242	19.478358229441593
20-21	27.904844446710303	30.11414038816752	22.022055507406087	19.958959657716093
22-23	36.617946374998525	22.95796355194064	21.689850309616343	18.73423976344449
24-25	20.0476091500551	39.092563325267754	22.045742432145197	18.81408509253195
26-27	28.390308753573628	30.445561250700997	21.418411485805493	19.74571850991988
28-29	28.057711388155752	30.30575349127231	21.70004666794775	19.93648845262419
30-31	27.337260238516354	22.188569882310492	30.008000219613873	20.466169659559284
32-33	19.12364260979713	30.35628429015699	30.425050883749748	20.095022216296133
34-35	27.714250979438653	30.309577125646587	21.83359935370775	20.142572541207013
36-37	19.69061895816748	22.208942990591897	30.03213813712533	28.06829991411529
38-39	19.67765781805775	22.2197276003655	29.80383775240889	28.29877682916786
40-41	35.65507680602997	21.806814304706403	21.801735733922108	20.73637315534152
42-43	27.26084637617503	21.912013270952535	30.447227963120554	20.379912389751876
44-45	27.147490323263774	22.217315769452494	30.436521786872582	20.19867212041115
46-47	27.675132258532585	22.224472828665885	21.72634150741393	28.3740534053876
48-49	19.77715074531458	22.266787715741216	22.16951053558333	35.78655100336088
50-51	27.86925523445742	22.46367547344437	30.02107901001204	19.645990282086174
52-53	19.676030322401004	30.247889157741586	30.411697576011893	19.664382943845517
54-55	19.09048483683866	30.459443984736833	22.408595530073374	28.04147564835113
56-57	19.01014929821603	22.648994286117656	38.43601589063229	19.90484052503402
58-59	19.759503202048684	38.533155812120334	21.92379790817787	19.783543077653114
60-61	20.185926672496894	38.84759581635574	21.59976940543466	19.366708105712707
62-63	19.71140384245842	38.232186765911216	22.63330758099242	19.423101810637938
64-65	19.62734271136855	38.433427584286626	22.12219551124933	19.8170341930955
66-67	19.39570890181299	38.60172632189903	22.50281380273184	19.499750973556136
68-69	20.41187405144455	34.55516425941104	23.79761405215045	21.235347636993957
70-71	21.707380202593797	32.186314918448744	24.130838885773333	21.975465993184127
72-73	21.19511123834772	31.64745698901538	25.065335126846623	22.092096645790278
74-75	20.890240124238705	31.218288345170258	25.44403179695129	22.447439733639747
76	20.69405826826619	31.54910134788014	25.413756456059577	22.343083927794098
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	698.0
1	418.0
2	140.5
3	148.5
4	154.0
5	130.0
6	113.5
7	128.0
8	135.0
9	147.5
10	151.5
11	158.0
12	173.0
13	182.0
14	239.5
15	325.5
16	493.5
17	624.0
18	729.5
19	1024.5
20	1525.0
21	1836.0
22	2250.5
23	3275.0
24	4688.5
25	5492.0
26	6668.0
27	9192.5
28	11980.5
29	13420.0
30	15592.5
31	20499.5
32	26592.5
33	29951.0
34	34255.5
35	43449.0
36	55038.5
37	61739.0
38	70013.5
39	123353.0
40	195307.5
41	224605.0
42	227013.0
43	215793.0
44	191348.0
45	165249.0
46	152375.0
47	143591.5
48	127855.0
49	112062.0
50	103222.0
51	96380.0
52	81893.0
53	75302.5
54	76357.0
55	71663.0
56	61051.5
57	44048.0
58	32962.0
59	35288.5
60	36741.5
61	36423.5
62	36979.0
63	36821.5
64	36684.0
65	33195.0
66	25064.0
67	20442.0
68	14479.0
69	8876.5
70	13041.0
71	16845.0
72	14772.5
73	11086.5
74	5762.0
75	2051.0
76	1339.5
77	396.0
78	120.5
79	77.0
80	57.0
81	31.0
82	20.0
83	15.0
84	14.5
85	17.0
86	18.5
87	17.0
88	17.5
89	17.0
90	12.5
91	7.5
92	6.0
93	7.5
94	11.0
95	12.0
96	11.0
97	16.5
98	23.0
99	83.0
100	142.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.3530057687858098E-4
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	2549930.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.76375168784657
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.62855108224576	34.89911031633915
2	15.997490227130218	14.962053212205396
3	4.5740068704783905	6.416931645286669
4	1.7916313276651414	3.3513361009239824
5	0.8683983711188069	2.030478289656516
6	0.6309978441757287	1.7704695900360168
7	0.2574988252358507	0.8429127786269067
8	0.18901272721172532	0.7071155392937443
9	0.16133486386088236	0.6790161160964552
>10	0.788333537470128	6.698254495509512
>50	0.059775080109363485	1.937925982942388
>100	0.0423106451525409	4.195524898825238
>500	0.007051179678722766	2.216048851717392
>1k	0.0031895286857136452	2.8253249623899213
>5k	1.6715591244777946E-4	0.43727389435843644
>10k+	2.507338685072303E-4	16.030223325792313
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	388759	15.24586949445671	Illumina Single End PCR Primer 1 (100% over 50bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG	11037	0.4328354111681497	No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC	10350	0.40589349511555217	No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG	6133	0.24051640633272284	No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG	5055	0.19824073602020448	No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA	4767	0.1869463083300326	No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA	3887	0.1524355570545074	No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC	3242	0.12714074504005993	No Hit
GCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTC	3084	0.12094449651559062	No Hit
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA	2935	0.1151011988564392	No Hit
GGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCT	2816	0.11043440408168068	No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC	2555	0.1001988289874624	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.009961057754526595	0.0	0.0
2	0.010666959485162338	0.0	0.0
3	0.012549364100190986	0.0	0.0
4	0.01372586698458389	0.0	0.0
5	0.015608271599612538	0.0	0.0
6	0.017137725349323316	7.843352562619367E-5	0.0
7	0.018471095284968608	7.843352562619367E-5	0.0
8	0.02141235249595087	7.843352562619367E-5	0.0
9	0.024471259995372422	7.843352562619367E-5	0.0
10	0.03137341025047746	7.843352562619367E-5	0.0
11	0.036040205025235986	7.843352562619367E-5	0.0
12	0.03945206338997541	7.843352562619367E-5	3.921676281309683E-5
13	0.04709933213852929	7.843352562619367E-5	3.921676281309683E-5
14	0.05415834944488672	7.843352562619367E-5	3.921676281309683E-5
15	0.05741334075837376	7.843352562619367E-5	3.921676281309683E-5
16	0.06788421642947061	7.843352562619367E-5	3.921676281309683E-5
17	0.07019800543544333	7.843352562619367E-5	3.921676281309683E-5
18	0.07255101120422913	7.843352562619367E-5	7.843352562619367E-5
19	0.07859039267744605	7.843352562619367E-5	7.843352562619367E-5
20	0.08019827995278302	7.843352562619367E-5	7.843352562619367E-5
21	0.08345327126627006	7.843352562619367E-5	7.843352562619367E-5
22	0.08612001113756064	7.843352562619367E-5	7.843352562619367E-5
23	0.08882596777166432	7.843352562619367E-5	7.843352562619367E-5
24	0.09106132325201084	7.843352562619367E-5	7.843352562619367E-5
25	0.09341432902079665	7.843352562619367E-5	7.843352562619367E-5
26	0.09623793594333963	7.843352562619367E-5	7.843352562619367E-5
27	0.09949292725682665	7.843352562619367E-5	7.843352562619367E-5
28	0.10302243591000537	7.843352562619367E-5	1.1765028843929049E-4
29	0.1089833838575961	7.843352562619367E-5	1.1765028843929049E-4
30	0.1237288866753205	7.843352562619367E-5	1.1765028843929049E-4
31	0.12659171036067657	7.843352562619367E-5	1.1765028843929049E-4
32	0.1309447710329303	7.843352562619367E-5	1.1765028843929049E-4
33	0.13321934327608992	7.843352562619367E-5	1.1765028843929049E-4
34	0.13592529991019361	7.843352562619367E-5	1.1765028843929049E-4
35	0.13831752244179252	7.843352562619367E-5	1.1765028843929049E-4
36	0.14137642994121408	7.843352562619367E-5	1.1765028843929049E-4
37	0.14443533744063564	7.843352562619367E-5	1.1765028843929049E-4
38	0.14796484609381433	7.843352562619367E-5	1.1765028843929049E-4
39	0.1509061033047966	7.843352562619367E-5	1.1765028843929049E-4
40	0.15565133160518133	7.843352562619367E-5	1.1765028843929049E-4
41	0.15878867263022906	7.843352562619367E-5	1.1765028843929049E-4
42	0.16239661480903397	7.843352562619367E-5	1.1765028843929049E-4
43	0.16686732576972702	7.843352562619367E-5	1.1765028843929049E-4
44	0.17125960320479386	7.843352562619367E-5	1.1765028843929049E-4
45	0.17616169855643096	7.843352562619367E-5	1.1765028843929049E-4
46	0.1803186754146192	7.843352562619367E-5	1.1765028843929049E-4
47	0.18365210025373246	7.843352562619367E-5	1.1765028843929049E-4
48	0.18824046150286478	7.843352562619367E-5	1.1765028843929049E-4
49	0.19239743836105305	7.843352562619367E-5	1.1765028843929049E-4
50	0.19808386896895208	7.843352562619367E-5	1.1765028843929049E-4
51	0.2039271666281035	7.843352562619367E-5	1.1765028843929049E-4
52	0.20851552787723585	7.843352562619367E-5	1.1765028843929049E-4
53	0.21424117524794797	7.843352562619367E-5	1.1765028843929049E-4
54	0.22094724168898755	7.843352562619367E-5	1.1765028843929049E-4
55	0.22847686014910212	7.843352562619367E-5	1.1765028843929049E-4
56	0.23475154219919764	7.843352562619367E-5	1.1765028843929049E-4
57	0.24181055950555505	7.843352562619367E-5	1.1765028843929049E-4
58	0.2509480652410066	7.843352562619367E-5	1.1765028843929049E-4
59	0.2614581576749166	7.843352562619367E-5	1.1765028843929049E-4
60	0.2708701807500598	7.843352562619367E-5	1.1765028843929049E-4
61	0.2809881055558388	7.843352562619367E-5	1.1765028843929049E-4
62	0.29271391763695476	7.843352562619367E-5	1.1765028843929049E-4
63	0.30126317193020985	7.843352562619367E-5	1.1765028843929049E-4
64	0.31110657939629716	7.843352562619367E-5	1.1765028843929049E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGCC	42020	0.0	68.46739	43
GCCGTAT	41920	0.0	68.330154	47
TGTAGGG	42745	0.0	68.1495	17
TGGTCGC	42255	0.0	68.10318	42
CGTATCA	42125	0.0	68.03086	49
CCGTATC	42080	0.0	68.012115	48
GTGGTCG	42500	0.0	67.924706	41
GAGCGTC	42865	0.0	67.90155	9
CGCCGTA	42155	0.0	67.89111	46
GTGTAGG	42985	0.0	67.8667	16
CGTGTAG	42890	0.0	67.85381	15
GTAGGGA	42990	0.0	67.83438	18
GTATCAT	42295	0.0	67.78224	50
GTCGTGT	42965	0.0	67.72722	13
TCGTGTA	42940	0.0	67.64439	14
CATTAAA	42550	0.0	67.622795	54
AGTGTAG	43075	0.0	67.56239	28
GAGTGTA	43080	0.0	67.55455	27
TAGGGAA	43145	0.0	67.5339	19
ATCTCGG	42840	0.0	67.524506	35
>>END_MODULE