FastQCFastQC Report
Mon 15 Sep 2014
TGA3_3+7_ACTTGA_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameTGA3_3+7_ACTTGA_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2549930
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG38875915.24586949445671Illumina Single End PCR Primer 1 (100% over 50bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG110370.4328354111681497No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC103500.40589349511555217No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG61330.24051640633272284No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG50550.19824073602020448No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA47670.1869463083300326No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA38870.1524355570545074No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC32420.12714074504005993No Hit
GCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTC30840.12094449651559062No Hit
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA29350.1151011988564392No Hit
GGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCT28160.11043440408168068No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC25550.1001988289874624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC420200.068.4673943
GCCGTAT419200.068.33015447
TGTAGGG427450.068.149517
TGGTCGC422550.068.1031842
CGTATCA421250.068.0308649
CCGTATC420800.068.01211548
GTGGTCG425000.067.92470641
GAGCGTC428650.067.901559
CGCCGTA421550.067.8911146
GTGTAGG429850.067.866716
CGTGTAG428900.067.8538115
GTAGGGA429900.067.8343818
GTATCAT422950.067.7822450
GTCGTGT429650.067.7272213
TCGTGTA429400.067.6443914
CATTAAA425500.067.62279554
AGTGTAG430750.067.5623928
GAGTGTA430800.067.5545527
TAGGGAA431450.067.533919
ATCTCGG428400.067.52450635
Produced by FastQC (version 0.11.2)