##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	TGA3_3+7_ACTTGA_L001_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2549930
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.78150772766311	34.0	34.0	34.0	34.0	34.0
2	33.797564639029304	34.0	34.0	34.0	34.0	34.0
3	33.736701007478636	34.0	34.0	34.0	34.0	34.0
4	33.782904628754515	34.0	34.0	34.0	34.0	34.0
5	33.83581157129804	34.0	34.0	34.0	34.0	34.0
6	37.56813716454962	38.0	38.0	38.0	37.0	38.0
7	37.530534563693905	38.0	38.0	38.0	37.0	38.0
8	37.492550383736024	38.0	38.0	38.0	37.0	38.0
9	37.53919558576118	38.0	38.0	38.0	37.0	38.0
10-11	37.55549250371578	38.0	38.0	38.0	37.0	38.0
12-13	37.479074327530554	38.0	38.0	38.0	37.0	38.0
14-15	37.463622726898386	38.0	38.0	38.0	37.0	38.0
16-17	37.49963685277635	38.0	38.0	38.0	37.0	38.0
18-19	37.46784813700768	38.0	38.0	38.0	37.0	38.0
20-21	37.479771993741004	38.0	38.0	38.0	37.0	38.0
22-23	37.241749773523196	38.0	38.0	38.0	37.0	38.0
24-25	37.424865780629275	38.0	38.0	38.0	37.0	38.0
26-27	37.429787484362315	38.0	38.0	38.0	37.0	38.0
28-29	37.491631338899495	38.0	38.0	38.0	37.0	38.0
30-31	37.34420258595334	38.0	38.0	38.0	37.0	38.0
32-33	37.32183236402568	38.0	38.0	38.0	37.0	38.0
34-35	37.12592777056625	38.0	38.0	38.0	36.5	38.0
36-37	37.174502437321806	38.0	38.0	38.0	37.0	38.0
38-39	36.87178275482072	38.0	38.0	38.0	36.5	38.0
40-41	36.90723215931418	38.0	37.5	38.0	35.0	38.0
42-43	36.9669073268678	38.0	37.5	38.0	36.0	38.0
44-45	37.27020643703945	38.0	38.0	38.0	37.0	38.0
46-47	37.28520861356978	38.0	38.0	38.0	37.0	38.0
48-49	37.30519308373171	38.0	38.0	38.0	37.0	38.0
50-51	37.21491217405968	38.0	38.0	38.0	37.0	38.0
52-53	37.294695736745716	38.0	38.0	38.0	37.0	38.0
54-55	37.25405364068817	38.0	38.0	38.0	37.0	38.0
56-57	37.20019412297593	38.0	38.0	38.0	37.0	38.0
58-59	37.303983246598925	38.0	38.0	38.0	37.0	38.0
60-61	37.15802492617445	38.0	38.0	38.0	37.0	38.0
62-63	37.209644578478624	38.0	38.0	38.0	37.0	38.0
64-65	37.192861764832756	38.0	38.0	38.0	37.0	38.0
66-67	37.24580027687034	38.0	38.0	38.0	37.0	38.0
68-69	37.20947124038699	38.0	38.0	38.0	37.0	38.0
70-71	37.183240912495634	38.0	38.0	38.0	37.0	38.0
72-73	37.01334193487664	38.0	38.0	38.0	36.0	38.0
74-75	34.85595722235512	38.0	37.0	38.0	25.5	38.0
76	34.07375104414631	38.0	36.0	38.0	22.0	38.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.0016224878421766675
1101	2	0.037823278378084524
1101	3	-0.04769566395732028
1101	4	0.04153131536288157
1101	5	0.023521218504704677
1101	6	0.05463547140773528
1101	7	-0.02281495939509881
1101	8	-0.0385348081881034
1101	9	0.04030439711620204
1101	10-11	0.020303892286747782
1101	12-13	0.04079655019247497
1101	14-15	-0.01859333772821259
1101	16-17	0.0935269271955903
1101	18-19	0.013350466212642687
1101	20-21	-0.00582653891086693
1101	22-23	0.25751699252995053
1101	24-25	0.036247894203903286
1101	26-27	0.024608959031873212
1101	28-29	-0.016392333515121038
1101	30-31	-0.01629787278358208
1101	32-33	0.04479040793597022
1101	34-35	0.07030796926292027
1101	36-37	0.041217748190248926
1101	38-39	0.11869503167780948
1101	40-41	0.09108888632800216
1101	42-43	0.05373323736073843
1101	44-45	0.1032713389665858
1101	46-47	0.0844455096808403
1101	48-49	0.058724111653489786
1101	50-51	0.07993232463292799
1101	52-53	-0.025025692551771783
1101	54-55	0.02070671198261209
1101	56-57	0.06952835951096858
1101	58-59	0.05110173000765883
1101	60-61	0.05937170661202629
1101	62-63	0.037275132673883604
1101	64-65	0.053739843110513164
1101	66-67	0.014292439786125044
1101	68-69	0.1653035500485629
1101	70-71	0.029622354170953713
1101	72-73	0.20339470726205633
1101	74-75	0.08990001243392243
1101	76	0.17077258893826297
1102	1	0.02872583144828411
1102	2	0.05268802892999247
1102	3	0.02633049316734315
1102	4	0.028556303675308925
1102	5	-0.01211696690799613
1102	6	0.02303639737297658
1102	7	-0.007020886270439064
1102	8	-0.15478603797540558
1102	9	0.013833219314875578
1102	10-11	0.04641986220286043
1102	12-13	0.08887355070408631
1102	14-15	0.031857208501975265
1102	16-17	0.030219907223532516
1102	18-19	0.06426483988212084
1102	20-21	-0.15327690334044064
1102	22-23	0.2565797264665761
1102	24-25	-0.08379699968411813
1102	26-27	-0.03944932424595038
1102	28-29	-0.11136015414934519
1102	30-31	-0.031622051134256424
1102	32-33	0.15064989526702988
1102	34-35	0.11733128329817788
1102	36-37	0.07317334671086684
1102	38-39	0.09698235585263859
1102	40-41	0.11051448418265153
1102	42-43	0.18059846276661773
1102	44-45	0.13098818556319003
1102	46-47	0.09882733111226827
1102	48-49	0.05050548440935643
1102	50-51	0.12516209942509704
1102	52-53	-0.03799628265547739
1102	54-55	0.0826039050009939
1102	56-57	0.13827541978856317
1102	58-59	0.10217721171069627
1102	60-61	0.040103969175987686
1102	62-63	0.06250836573710927
1102	64-65	0.057755760373567
1102	66-67	0.12288379667586469
1102	68-69	0.13313755874317934
1102	70-71	0.01582522411901266
1102	72-73	0.12789774263000453
1102	74-75	-0.16020878815275097
1102	76	0.014704283257387374
1103	1	6.900456715186465E-5
1103	2	-0.006118972682415347
1103	3	-0.07912933800962207
1103	4	-0.008179415114206279
1103	5	0.03588442102835643
1103	6	0.02842281689470383
1103	7	0.04966617550641672
1103	8	-0.008465550669228605
1103	9	0.04044782780285061
1103	10-11	0.042014095346672775
1103	12-13	0.05703538334355329
1103	14-15	0.13210272549791569
1103	16-17	0.12779715311457807
1103	18-19	0.14561187420048327
1103	20-21	0.017627045679219577
1103	22-23	0.3055181959568287
1103	24-25	-0.002883651454503422
1103	26-27	0.003407447880192649
1103	28-29	0.031505459863915064
1103	30-31	0.08832577446806766
1103	32-33	0.1434965620648896
1103	34-35	0.10843138574256983
1103	36-37	0.05394373899262206
1103	38-39	0.020491370901631
1103	40-41	0.03948473472127745
1103	42-43	0.11443167418007505
1103	44-45	0.09108192277332705
1103	46-47	0.06483100151894661
1103	48-49	-0.02493267324864945
1103	50-51	0.036017751094625794
1103	52-53	-0.12317056424059558
1103	54-55	0.03641569822506341
1103	56-57	-0.020755296755588404
1103	58-59	-0.03723980219319145
1103	60-61	-0.03747294786818145
1103	62-63	0.07519453676592747
1103	64-65	-0.15913532338117875
1103	66-67	-0.019643571683147343
1103	68-69	-0.1197601017731671
1103	70-71	-0.025307765214947153
1103	72-73	-0.24186985550976914
1103	74-75	0.1287365219690102
1103	76	-0.13719929337730008
2116	1	-0.02924471511504123
2116	2	0.003399730962023284
2116	3	0.07382532739252667
2116	4	0.02864426285427868
2116	5	0.022948119230548514
2116	6	-0.10349886067194802
2116	7	0.01121635996417325
2116	8	-0.07549183465138753
2116	9	0.013405713788692708
2116	10-11	0.02880766425776926
2116	12-13	0.04005085840515221
2116	14-15	0.14608057313056833
2116	16-17	0.12572128683401473
2116	18-19	0.13252295649140677
2116	20-21	-0.020033229907362227
2116	22-23	0.3658177189590859
2116	24-25	0.08518344514352805
2116	26-27	0.06258030059154862
2116	28-29	0.015190749202709242
2116	30-31	0.061832550270153774
2116	32-33	0.03901023186900687
2116	34-35	0.021343138654223992
2116	36-37	-0.04575563468927868
2116	38-39	-0.05783061562832614
2116	40-41	-0.12352552541845085
2116	42-43	-0.058492855137593835
2116	44-45	-0.05011391770420204
2116	46-47	-0.09209105638870341
2116	48-49	-0.008276658309462448
2116	50-51	-0.019706269730015435
2116	52-53	-0.1793512340165151
2116	54-55	0.009512850044245624
2116	56-57	-0.045521064461794936
2116	58-59	-0.16079299703358174
2116	60-61	-0.17113381909710057
2116	62-63	-0.0543686887890118
2116	64-65	-0.1043484889250692
2116	66-67	-0.030493991917438734
2116	68-69	-0.17529140196265303
2116	70-71	-0.14360298009802364
2116	72-73	0.01768605398450518
2116	74-75	-0.007890562207009566
2116	76	-0.366522158680219
1117	1	-0.009233068367180408
1117	2	0.02974122915819777
1117	3	0.03001863763833512
1117	4	-0.019305774104950046
1117	5	-0.04516105518217728
1117	6	-0.12721644421543488
1117	7	-0.11702500082964207
1117	8	-0.07120465607035698
1117	9	0.008120633822258583
1117	10-11	-4.760664458913766E-4
1117	12-13	0.07607127255625556
1117	14-15	0.0740852235030971
1117	16-17	-0.023819574665090215
1117	18-19	0.16552135598389128
1117	20-21	-0.011565146690088568
1117	22-23	0.3921464971501152
1117	24-25	0.034274894946698
1117	26-27	0.11933651434144821
1117	28-29	0.09021009653870493
1117	30-31	0.07869955099198478
1117	32-33	-0.018297094455157037
1117	34-35	0.061856596069070235
1117	36-37	0.07844869666858045
1117	38-39	0.17225657239246317
1117	40-41	0.20476635822783606
1117	42-43	0.2613457688633076
1117	44-45	0.18515348967213896
1117	46-47	0.10442271340580334
1117	48-49	0.1087723140828416
1117	50-51	0.22460199360165944
1117	52-53	0.156978093293084
1117	54-55	0.274560561348288
1117	56-57	0.14976435382828868
1117	58-59	0.22783079131745865
1117	60-61	0.154564936472255
1117	62-63	0.2521181588571437
1117	64-65	0.21372581229526588
1117	66-67	0.3272936663425412
1117	68-69	0.23975020461541163
1117	70-71	0.3012536611711454
1117	72-73	0.32056966709878765
1117	74-75	0.17570835957479147
1117	76	-0.026429846283207326
2117	1	0.03407566430968956
2117	2	0.05315622999685132
2117	3	-0.02372384407144068
2117	4	0.00587282508028153
2117	5	-0.03934673510609343
2117	6	-0.013597240950019795
2117	7	0.02247797750661107
2117	8	-0.07975432091422618
2117	9	0.054216260238867164
2117	10-11	-0.009465544132744697
2117	12-13	0.10818031048733445
2117	14-15	0.10430251515347777
2117	16-17	-0.008692278562349998
2117	18-19	0.1261122327818427
2117	20-21	0.003172853684020538
2117	22-23	0.3547616810007952
2117	24-25	0.0923205302768011
2117	26-27	0.08607405718549899
2117	28-29	0.08864031178859477
2117	30-31	0.19250514726078194
2117	32-33	0.18428257153912142
2117	34-35	0.06219568204520698
2117	36-37	0.14372960782959154
2117	38-39	0.0853569269123966
2117	40-41	0.19296541980821758
2117	42-43	0.26543585486314925
2117	44-45	0.1292278387456136
2117	46-47	-0.08059315524706534
2117	48-49	0.04518861111030503
2117	50-51	0.1799240517316889
2117	52-53	0.11813016890760508
2117	54-55	0.22114484918650135
2117	56-57	-0.04797225551392614
2117	58-59	0.1592024242782344
2117	60-61	0.08343773960348244
2117	62-63	0.18398159272121717
2117	64-65	0.18514410278382343
2117	66-67	0.11346514966201227
2117	68-69	0.07484019138128417
2117	70-71	0.16395086672508796
2117	72-73	0.23278881521470396
2117	74-75	0.07586612401794923
2117	76	-0.18988922139023146
1116	1	-0.026693855582330173
1116	2	0.02914933806615494
1116	3	0.017736897478528135
1116	4	0.06119141441236309
1116	5	0.05161313836620707
1116	6	-0.02600306747675063
1116	7	0.007864019590599014
1116	8	0.07395663425221954
1116	9	0.021586156166151227
1116	10-11	0.0815748361880182
1116	12-13	0.1306732257968548
1116	14-15	0.04412073696862251
1116	16-17	-0.1078681097346319
1116	18-19	0.0023073873541150647
1116	20-21	-0.043601252046698846
1116	22-23	0.33924623079912664
1116	24-25	0.07770490382506523
1116	26-27	-0.0013352820480179162
1116	28-29	0.006679466174695392
1116	30-31	0.12198158709734486
1116	32-33	0.15853036929151898
1116	34-35	0.06459409236975233
1116	36-37	0.07223384020216628
1116	38-39	0.08561851379524654
1116	40-41	0.002931495842510401
1116	42-43	0.030064324649046625
1116	44-45	0.026452690619159114
1116	46-47	0.0841504435034679
1116	48-49	0.056094006891370896
1116	50-51	0.14373487315182132
1116	52-53	0.07707279586780658
1116	54-55	0.1398313515228935
1116	56-57	0.021102027696301207
1116	58-59	0.07386512100079301
1116	60-61	0.09278630374068086
1116	62-63	0.0875724352702818
1116	64-65	0.11132865337251019
1116	66-67	0.12960204160148692
1116	68-69	0.06684401935349626
1116	70-71	0.10911101541796597
1116	72-73	0.17762796837136108
1116	74-75	0.034690306896486334
1116	76	-0.2778355876267966
2118	1	0.014267511400738897
2118	2	0.03028948556976019
2118	3	0.051748708515589215
2118	4	-0.010735126318571986
2118	5	0.02846316785134917
2118	6	0.007103006637080966
2118	7	0.07025522283901608
2118	8	0.06564258639727427
2118	9	0.09535043033059765
2118	10-11	0.10818964979878132
2118	12-13	0.07581937746750356
2118	14-15	-0.02213596502507187
2118	16-17	-0.11534272612365015
2118	18-19	0.015191653738718003
2118	20-21	-0.0322471974482923
2118	22-23	0.19471061890391184
2118	24-25	-0.01916023542645462
2118	26-27	0.02769495402829847
2118	28-29	-0.05491197946722082
2118	30-31	0.09449699031461734
2118	32-33	-0.04649838101736492
2118	34-35	0.0567205272045328
2118	36-37	-0.0037752785269660194
2118	38-39	-0.10490654139584166
2118	40-41	-0.2836151750151785
2118	42-43	-0.04520611189645507
2118	44-45	-0.18704280704334053
2118	46-47	-0.035780482403446
2118	48-49	-0.29941381894700214
2118	50-51	-0.0028083935880758304
2118	52-53	-0.15532809454226992
2118	54-55	-0.07490797524606307
2118	56-57	-0.07966333860437658
2118	58-59	-0.1468510847269613
2118	60-61	-0.18472004473332504
2118	62-63	-0.07024733275427764
2118	64-65	-0.3273323650320492
2118	66-67	-0.07433129003372585
2118	68-69	-0.282252046881581
2118	70-71	0.03758810642293042
2118	72-73	-0.1260144157925751
2118	74-75	-0.0891726201598928
2118	76	-0.6169778042107978
1119	1	-0.017152963327255577
1119	2	0.014595843561501454
1119	3	-0.03439644107763229
1119	4	0.028286806915332363
1119	5	0.011209496939024177
1119	6	0.07298624158319456
1119	7	0.07293326154684365
1119	8	0.14352383801835344
1119	9	0.07906315248087026
1119	10-11	0.1031890468669232
1119	12-13	0.02088966649116486
1119	14-15	-0.08604484362550124
1119	16-17	0.04310858480470614
1119	18-19	-0.0021066315125963797
1119	20-21	0.06032549381039587
1119	22-23	0.2709030721347929
1119	24-25	0.12042146864882142
1119	26-27	0.09360889201914802
1119	28-29	0.13897820628161384
1119	30-31	0.16074630506061993
1119	32-33	0.19452772128413898
1119	34-35	0.17230842373697186
1119	36-37	0.1986725462637864
1119	38-39	0.17161607758303887
1119	40-41	0.1875654349580813
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2101	32-33	0.05898173223203429
2101	34-35	0.14580203761560284
2101	36-37	0.16839289833385607
2101	38-39	0.18410345285234797
2101	40-41	0.01289130960098106
2101	42-43	0.09987238860418302
2101	44-45	0.06018524082184484
2101	46-47	0.015873585065683926
2101	48-49	0.1325755831315547
2101	50-51	0.10710627782268745
2101	52-53	0.13189459305737472
2101	54-55	0.0676079010479782
2101	56-57	0.1696368719275938
2101	58-59	0.07547794605576996
2101	60-61	0.00637133088250863
2101	62-63	-0.013302277865783196
2101	64-65	0.07600817782167013
2101	66-67	0.010510008606416932
2101	68-69	-0.05247670928687853
2101	70-71	0.024679972109666437
2101	72-73	0.2930333503130882
2101	74-75	0.19428781727527422
2101	76	0.2625642441863718
2107	1	0.016708066286966528
2107	2	-0.0487375533049601
2107	3	0.02986415908846851
2107	4	-0.04517996655857104
2107	5	-0.028227056423027364
2107	6	-0.10202831958233816
2107	7	-0.2810258095201448
2107	8	-0.21032270783337026
2107	9	-0.15665184973876478
2107	10-11	-0.071777115033548
2107	12-13	-0.40122796320789433
2107	14-15	0.002523809238738295
2107	16-17	-0.14023531426602887
2107	18-19	-0.045827554482443134
2107	20-21	-0.04302252954438046
2107	22-23	0.192819185504014
2107	24-25	0.014952311430022291
2107	26-27	0.011437689132193896
2107	28-29	-0.0289110591528825
2107	30-31	-0.0081034402655078
2107	32-33	-0.06461569069336548
2107	34-35	-0.04130153684295834
2107	36-37	-0.2612359499313399
2107	38-39	-0.2135579356155617
2107	40-41	-0.06772377587372347
2107	42-43	-0.15282878893679452
2107	44-45	-0.11123068031007932
2107	46-47	-0.22252734609737956
2107	48-49	-0.07959751950028249
2107	50-51	-0.2993009708969012
2107	52-53	-0.07430072617672323
2107	54-55	-0.22933529973929012
2107	56-57	-0.02544830880042781
2107	58-59	-0.038390667839458104
2107	60-61	-0.06618639191764686
2107	62-63	-0.2523791276506273
2107	64-65	0.02463143707328186
2107	66-67	-0.3526392963379905
2107	68-69	-0.07914630884454255
2107	70-71	-0.27224353578124294
2107	72-73	0.04437402196710849
2107	74-75	-0.13363518331155433
2107	76	0.0999428502006765
2106	1	0.059406120203945534
2106	2	0.0866108361920439
2106	3	0.036076122699853386
2106	4	0.016936018573169065
2106	5	0.02209671267571167
2106	6	-0.02451349568197969
2106	7	-0.4409387372463982
2106	8	-0.11459568957955213
2106	9	-0.046916195451721876
2106	10-11	0.02665655885123641
2106	12-13	0.1049385913127665
2106	14-15	0.03912266925605934
2106	16-17	-0.04307496604995009
2106	18-19	-0.10094624973825006
2106	20-21	-0.1314804422997753
2106	22-23	0.11031328642958016
2106	24-25	0.04512799851748639
2106	26-27	-0.02107038227663338
2106	28-29	-0.014095875521256573
2106	30-31	-0.015398148276382528
2106	32-33	-0.009474496259940679
2106	34-35	0.006589856660262683
2106	36-37	-0.080723815709554
2106	38-39	-0.22433526988014307
2106	40-41	0.019775982248710022
2106	42-43	-0.07613785480014457
2106	44-45	-0.02585588508454606
2106	46-47	-0.12032302910304082
2106	48-49	-0.0718181436064711
2106	50-51	-0.2106256421533388
2106	52-53	0.009809460100036915
2106	54-55	-0.27179284520588
2106	56-57	0.007849333139716919
2106	58-59	0.06336918771307865
2106	60-61	0.03249746552026522
2106	62-63	-0.10387063601375246
2106	64-65	-0.03618256217973226
2106	66-67	-0.1500232033197335
2106	68-69	0.12652141198236677
2106	70-71	-0.12524623130138224
2106	72-73	0.08239909253146749
2106	74-75	0.042139390810497446
2106	76	0.21275299657220614
2105	1	0.003866561723206985
2105	2	-0.04290340362605605
2105	3	-0.026246770612516457
2105	4	-0.09465284056146572
2105	5	-0.053623178392832926
2105	6	-0.0939706000631233
2105	7	-0.03323072003311722
2105	8	-0.019101143870749127
2105	9	-0.12372435677488625
2105	10-11	-0.05734218166289651
2105	12-13	0.03979767781773802
2105	14-15	-0.06449310855137469
2105	16-17	-0.024250101782939737
2105	18-19	-0.16201374347033237
2105	20-21	-0.09621599566992245
2105	22-23	0.24063293431837707
2105	24-25	0.10011309263386181
2105	26-27	0.009497507825734886
2105	28-29	-0.022143554371432117
2105	30-31	-0.03929639325955492
2105	32-33	-0.08295664325202523
2105	34-35	-0.010034335275577178
2105	36-37	-0.19071379975485314
2105	38-39	-0.08231911177516338
2105	40-41	-0.06779024972734504
2105	42-43	-0.3373085046930413
2105	44-45	-0.2558233627438824
2105	46-47	-0.28800137075878496
2105	48-49	-0.22818874028676817
2105	50-51	-0.42721260343254386
2105	52-53	-0.1908255042084761
2105	54-55	-0.6569779269725728
2105	56-57	-0.301520442650002
2105	58-59	-0.1938640705232899
2105	60-61	-0.13376969803945826
2105	62-63	-0.6482989547408664
2105	64-65	-0.6933884195608897
2105	66-67	-0.8707886020555193
2105	68-69	-0.23755972179101548
2105	70-71	-0.9534209393358424
2105	72-73	-0.4593473476275136
2105	74-75	-0.451892557633208
2105	76	-0.12263960070196589
2104	1	-0.0029644977180964815
2104	2	0.023803400450631784
2104	3	-0.06808416157311825
2104	4	-0.034681354285289956
2104	5	-0.038353228495005
2104	6	-0.2070072218580492
2104	7	-0.032978491879326555
2104	8	0.07658217645384013
2104	9	0.058125564779828665
2104	10-11	0.005324448637658463
2104	12-13	0.10107236159533528
2104	14-15	-0.02236665191470877
2104	16-17	-0.026970993910957475
2104	18-19	-0.030540244823846763
2104	20-21	0.003177711691094487
2104	22-23	0.2552918662277719
2104	24-25	0.18702477259772365
2104	26-27	0.044661302355422094
2104	28-29	0.11812733273941234
2104	30-31	-0.15409435819409367
2104	32-33	-0.05592594958194752
2104	34-35	-0.1008732071449785
2104	36-37	-0.21158917017847756
2104	38-39	-0.14174469111311083
2104	40-41	0.10121676691149872
2104	42-43	-0.17542871803190252
2104	44-45	-0.17666140178447165
2104	46-47	-0.040814435261268045
2104	48-49	-0.10143267808994949
2104	50-51	-0.03368746580814985
2104	52-53	-0.2061427861695151
2104	54-55	-0.06752369473186093
2104	56-57	-0.2697098166972154
2104	58-59	-0.10317624147453586
2104	60-61	-0.1418870402515111
2104	62-63	-0.13418997044855274
2104	64-65	-0.5861781760808071
2104	66-67	-0.040721282100875555
2104	68-69	-0.4478004070721866
2104	70-71	-0.13287519851441232
2104	72-73	-0.6005956412825029
2104	74-75	-0.054947905729839874
2104	76	-0.2703974616743281
2111	1	0.03047557518281252
2111	2	-0.05996885179119715
2111	3	-0.0077935646303899375
2111	4	-0.03718111436295857
2111	5	0.00672505093282183
2111	6	-0.07180575539599943
2111	7	0.023846269559818722
2111	8	0.08133525513186868
2111	9	0.006146458333958549
2111	10-11	-0.024935555768749396
2111	12-13	0.07530221978305462
2111	14-15	-0.12947581888131765
2111	16-17	-0.06574227626344253
2111	18-19	-0.027701433692648436
2111	20-21	0.03205796312969511
2111	22-23	0.29932113959039697
2111	24-25	0.0724194186666125
2111	26-27	0.04053327456664846
2111	28-29	0.06020830816692779
2111	30-31	-0.05325907148202447
2111	32-33	0.0038671854818161933
2111	34-35	-0.07031025090450527
2111	36-37	-0.08015038212595016
2111	38-39	0.07772963817314604
2111	40-41	-0.12024657836680319
2111	42-43	-0.0015574040327379635
2111	44-45	-0.06654486827100214
2111	46-47	0.09665619310107587
2111	48-49	-0.15254647870624183
2111	50-51	0.09067975567484865
2111	52-53	-0.020112776098990537
2111	54-55	0.14020743429028926
2111	56-57	-0.06828009691053438
2111	58-59	0.09748763381480785
2111	60-61	-0.11907660204246895
2111	62-63	0.07238152658341335
2111	64-65	-0.22127015857720522
2111	66-67	0.08992946531262191
2111	68-69	-0.1352555934834072
2111	70-71	0.10443073713743445
2111	72-73	-0.22547204358640016
2111	74-75	0.2908662301213738
2111	76	0.13168708642287186
2110	1	0.008694542096606028
2110	2	-0.008793413898352753
2110	3	0.029004845536725554
2110	4	0.008693986887088556
2110	5	0.011942774509066112
2110	6	0.027525536515419446
2110	7	0.011868312208214604
2110	8	0.05702508513640936
2110	9	0.058533474611564884
2110	10-11	-0.07441852657822068
2110	12-13	-0.04944647519206313
2110	14-15	-0.13620633157059103
2110	16-17	-0.12121260182431826
2110	18-19	-0.1943457759400573
2110	20-21	-0.07566924678073406
2110	22-23	0.19202841119687264
2110	24-25	-0.10152551002457244
2110	26-27	-0.12359976124339056
2110	28-29	-0.058666706764448406
2110	30-31	-0.3086151555965202
2110	32-33	-0.10162469794787654
2110	34-35	-0.04239422763159695
2110	36-37	-0.06386608244498149
2110	38-39	0.06407708780533738
2110	40-41	-0.13237945156642894
2110	42-43	0.02829551419210219
2110	44-45	0.008199042121503908
2110	46-47	0.12652736685183896
2110	48-49	0.02225414915306345
2110	50-51	0.1342334515523902
2110	52-53	0.0030749460734895706
2110	54-55	0.11881144566692114
2110	56-57	-0.08121715054929979
2110	58-59	0.05527734846622678
2110	60-61	-0.023703838371631036
2110	62-63	0.1221499811218223
2110	64-65	0.08285802071799253
2110	66-67	0.17981113026890228
2110	68-69	0.06809693848718723
2110	70-71	0.185277104734638
2110	72-73	0.057503801137656296
2110	74-75	0.33951087448411243
2110	76	0.09200587259060455
2109	1	0.045079863042474244
2109	2	-0.047415807753125705
2109	3	0.01510556120281592
2109	4	0.001067538764189635
2109	5	0.05706904747790986
2109	6	0.04823750591763343
2109	7	0.01916079637188517
2109	8	0.10730263077193314
2109	9	0.035525174074379606
2109	10-11	0.014643228515659246
2109	12-13	0.05185470281654858
2109	14-15	-0.08565895777430654
2109	16-17	-0.02309737709177284
2109	18-19	0.001868586093259239
2109	20-21	-0.17277344488375945
2109	22-23	0.092999176461241
2109	24-25	-0.049439700690378174
2109	26-27	-0.14578260752723082
2109	28-29	-0.13078543329448422
2109	30-31	-0.7536551404589247
2109	32-33	-0.10233588223208301
2109	34-35	-0.05735446953351442
2109	36-37	-0.016334171515872242
2109	38-39	-0.0233983117411114
2109	40-41	-0.2715938607710058
2109	42-43	-0.045102879824405306
2109	44-45	-0.028733775629611102
2109	46-47	0.0843220109992373
2109	48-49	0.0049550471077282054
2109	50-51	0.10398230898640293
2109	52-53	0.01496282009691896
2109	54-55	-0.003426198431384364
2109	56-57	0.09520248240696105
2109	58-59	0.11121993772767524
2109	60-61	-0.03621034046445004
2109	62-63	0.054403880585851994
2109	64-65	0.11347478867808292
2109	66-67	0.14103382000833165
2109	68-69	0.008352441987973691
2109	70-71	0.11261405823928072
2109	72-73	-0.4423864320905224
2109	74-75	0.1450701090175741
2109	76	-0.3194225576450762
2108	1	0.03245388465897747
2108	2	-0.052299122355016436
2108	3	-0.058157410156503886
2108	4	0.019287440365424402
2108	5	0.004433646814113956
2108	6	-0.0819742669662844
2108	7	-0.12021227480268237
2108	8	0.027673184575199627
2108	9	-0.043011018327277384
2108	10-11	0.013344637685129612
2108	12-13	0.06462296381413779
2108	14-15	-0.09067530376243838
2108	16-17	-0.042642977526419656
2108	18-19	0.0022490401922112824
2108	20-21	0.00508636421110964
2108	22-23	0.1192476301883687
2108	24-25	0.0021182283073102326
2108	26-27	-0.030759561914209144
2108	28-29	-0.08139140247883603
2108	30-31	-0.04320502334410037
2108	32-33	-0.037783407627067334
2108	34-35	-0.19428878367764923
2108	36-37	-0.3075647505794379
2108	38-39	-0.3305076284198023
2108	40-41	-0.42832156853500436
2108	42-43	-0.12336064625663568
2108	44-45	-0.27825002850212144
2108	46-47	-0.2675024168700091
2108	48-49	-0.30379971793617244
2108	50-51	-0.18753810659992354
2108	52-53	-0.10038226139253226
2108	54-55	-0.23672619206136858
2108	56-57	-0.23141120640836732
2108	58-59	-0.08745544098466951
2108	60-61	-0.4034020316001161
2108	62-63	-0.15078768619807192
2108	64-65	-0.07596847856937927
2108	66-67	-0.1365439367204857
2108	68-69	-0.48839169739623856
2108	70-71	-0.20837663055866074
2108	72-73	-1.4305499835526376
2108	74-75	-0.4507811640942734
2108	76	-0.8035584272318133
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	1.0
15	3.0
16	7.0
17	26.0
18	54.0
19	120.0
20	218.0
21	359.0
22	545.0
23	847.0
24	1306.0
25	1911.0
26	3171.0
27	5082.0
28	6119.0
29	8548.0
30	12825.0
31	19692.0
32	29987.0
33	45900.0
34	74282.0
35	141878.0
36	329171.0
37	1867877.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.67895589290686	11.430313773319266	11.551964171565494	29.338766162208373
2	24.17748722513951	32.508343366288486	23.82779135113513	19.48637805743687
3	23.61127560364402	20.037687309063386	34.54765424933233	21.803382837960257
4	24.76311114422749	25.485170181142223	15.485601565533171	34.26611710909711
5	40.52444576909954	26.141109755954083	17.87786331389489	15.45658116105148
6	34.068268540705034	29.86093735906476	19.68022651602201	16.390567584208192
7	15.634076229543556	32.765683763868026	33.566372410223025	18.03386759636539
8	18.77008388465566	34.9919017384791	24.29074523614374	21.94726914072151
9	34.930919672304725	18.718121673928305	26.287976532689132	20.062982121077834
10-11	28.580294361021675	34.36111971701184	18.955912515245515	18.102673406720967
12-13	19.169918389916585	29.55938006141345	23.8624393610805	27.40826218758946
14-15	19.20893906891562	29.900036471589413	22.92929609832427	27.9617283611707
16-17	28.228872949453514	22.11390116591436	21.976603279305706	27.68062260532642
18-19	19.923939088523998	22.312357594130034	30.571819618577766	27.191883698768198
20-21	28.07324122622974	21.83899165859455	30.344597694838683	19.74316942033703
22-23	19.959940076786424	38.8926754852094	21.942072919648776	19.205311518355405
24-25	20.129493750808848	22.750114709031227	30.287772605522505	26.83261893463742
26-27	20.342401556121146	22.16166718302071	21.58839654421886	35.907534716639276
28-29	28.142086253348133	30.292243316483198	21.821442157235687	19.744228272932983
30-31	19.303824026541903	22.269454455612507	29.981960289105974	28.444761228739612
32-33	19.30186318840125	30.30714568635218	22.302259277705662	28.088731847540917
34-35	19.765934751150034	30.176651908091596	22.00964340197574	28.047769938782636
36-37	19.884055640743078	22.232806390763667	38.05947614248234	19.82366182601091
38-39	27.659994588086732	30.128317247924453	22.129352570462718	20.082335593526096
40-41	19.52700662371124	29.919272293749238	29.86715321597063	20.686567866568886
42-43	19.10554407375889	21.892581364978646	30.57178040181495	28.430094159447517
44-45	27.330083570921555	22.134019365237478	22.44896918738946	28.08692787645151
46-47	19.673347831773388	30.390369616101147	29.895081413035147	20.04120113909032
48-49	27.96422410894105	22.39418776087731	30.256365911292843	19.3852222188888
50-51	19.925911648110915	22.23723135766356	22.009126922578417	35.82773007164711
52-53	27.908648472703174	22.044663971167836	30.51572003937363	19.530967516755364
54-55	19.270783903871873	22.237786919640932	30.71411764244509	27.77731153404211
56-57	19.1984093681003	22.420537034349962	30.349460573427507	28.03159302412223
58-59	28.01098461526395	22.202413399583516	30.100806688811065	19.685795296341468
60-61	20.308726303502237	22.526141419259403	29.938408076932355	27.22672420030601
62-63	28.111048625686685	21.83112621219109	30.779143567975254	19.27868159414697
64-65	19.876071010632458	38.399260685007576	22.171704455968953	19.552963848391013
66-67	19.444815914482323	38.673880723737796	22.579001219249633	19.30230214253024
68-69	19.714050973948304	38.403387543971796	22.159529869447397	19.723031612632504
70-71	20.347989944822015	38.483781907738646	21.709046915013353	19.459181232425987
72-73	20.027863509978705	38.264030777315455	22.22774742836078	19.480358284345062
74-75	19.622695524975196	36.0689901291408	23.534057797665035	20.77425654821897
76	19.981097520324088	35.340774060464405	23.71343527077214	20.96469314843937
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	823.0
1	746.5
2	571.5
3	420.5
4	368.0
5	319.0
6	234.5
7	200.5
8	202.0
9	185.0
10	200.5
11	245.0
12	257.0
13	278.5
14	318.0
15	367.5
16	516.5
17	634.0
18	756.5
19	1098.5
20	1610.0
21	1902.0
22	2212.5
23	3157.0
24	4753.0
25	5715.0
26	6652.0
27	8872.0
28	11726.0
29	13297.0
30	15342.0
31	19962.0
32	25945.0
33	29353.0
34	33715.0
35	42803.0
36	54601.0
37	61673.0
38	69638.0
39	88152.0
40	110430.0
41	273031.5
42	423904.0
43	320967.0
44	189792.0
45	150926.5
46	140299.0
47	134732.5
48	124522.5
49	112018.5
50	104158.0
51	97850.0
52	82637.5
53	74921.0
54	76109.0
55	72677.5
56	61666.0
57	43623.0
58	33160.0
59	34872.0
60	35371.5
61	35234.5
62	36310.0
63	36272.5
64	36240.0
65	32690.0
66	24662.5
67	20190.0
68	14032.5
69	8804.0
70	12834.0
71	15935.0
72	14386.0
73	11241.0
74	5743.5
75	1842.0
76	1205.5
77	352.0
78	88.0
79	41.0
80	34.0
81	22.0
82	12.0
83	7.0
84	7.0
85	7.0
86	4.0
87	1.0
88	2.0
89	1.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	1.9608381406548416E-5
48-49	1.9608381406548416E-5
50-51	5.8825144219645246E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	5.8825144219645246E-5
62-63	7.843352562619367E-5
64-65	7.843352562619367E-5
66-67	3.921676281309683E-5
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	2549930.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.14360614608768
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.19759079533449	33.776008005024075
2	17.3400035865858	16.002605921423257
3	4.734891704080508	6.554549338124066
4	1.7897929991193613	3.3035001293755544
5	0.8150059548736133	1.8803656894202059
6	0.4891479667593828	1.3542630675182727
7	0.30748080325035193	0.9931791157866842
8	0.2535770502140683	0.9360767626211732
9	0.17117777056499558	0.7108883663324864
>10	0.775859675162657	6.549376919381693
>50	0.06181507688351979	1.9546114106311832
>100	0.05197533452091977	4.837365873618735
>500	0.007205242039584606	2.2580595584083802
>1k	0.004047312302195055	3.43319101182435
>5k	2.5723698513768696E-4	0.8060018466389991
>10k+	1.7149132340518836E-4	14.649956983870865
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC	358294	14.051130815355716	TruSeq Adapter, Index 8 (100% over 50bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC	11971	0.4694638676355821	No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG	8751	0.34318589137741035	No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG	6285	0.2464773542803136	No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG	5335	0.2092214296078716	No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA	3838	0.15051393567666563	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAACCTCGTATGC	3633	0.14247449929998077	TruSeq Adapter, Index 8 (98% over 50bp)
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA	3551	0.13925872474930684	No Hit
GCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTC	3365	0.13196440686607083	No Hit
GGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCT	3271	0.12827803116163972	No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT	3144	0.12329750228437643	No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC	3024	0.1185914907468048	No Hit
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA	2760	0.10823826536414725	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGTATCTCGTATGC	2657	0.10419893879439829	TruSeq Adapter, Index 8 (98% over 50bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.006980583780731236	0.0	0.0
2	0.007764919036993173	0.0	0.0
3	0.009608106889208724	0.0	0.0
4	0.010745393010788532	0.0	3.921676281309683E-5
5	0.012549364100190986	0.0	3.921676281309683E-5
6	0.013882734035836277	0.0	3.921676281309683E-5
7	0.01521610397148157	0.0	3.921676281309683E-5
8	0.01756910974026738	0.0	3.921676281309683E-5
9	0.020588800476875835	0.0	3.921676281309683E-5
10	0.026432098136027263	0.0	3.921676281309683E-5
11	0.0296870894495143	0.0	3.921676281309683E-5
12	0.03145184377610366	0.0	3.921676281309683E-5
13	0.03737357496088128	0.0	3.921676281309683E-5
14	0.044550242555678	0.0	3.921676281309683E-5
15	0.04733463271540787	0.0	3.921676281309683E-5
16	0.05733490723274757	0.0	3.921676281309683E-5
17	0.05929574537340241	0.0	3.921676281309683E-5
18	0.06102128293717867	0.0	3.921676281309683E-5
19	0.06659006325663842	0.0	3.921676281309683E-5
20	0.06784499966665751	0.0	3.921676281309683E-5
21	0.07000192162137785	0.0	3.921676281309683E-5
22	0.07204119328765887	0.0	3.921676281309683E-5
23	0.07400203142831371	0.0	3.921676281309683E-5
24	0.07572756899208997	0.0	3.921676281309683E-5
25	0.07753154008149243	0.0	3.921676281309683E-5
26	0.07941394469652108	0.0	3.921676281309683E-5
27	0.08125713254873664	0.0	3.921676281309683E-5
28	0.08439447357378438	0.0	3.921676281309683E-5
29	0.08831614985509406	0.0	3.921676281309683E-5
30	0.10290478562156609	0.0	3.921676281309683E-5
31	0.10435580584565067	0.0	7.843352562619367E-5
32	0.107728447447577	0.0	7.843352562619367E-5
33	0.10953241853697945	0.0	7.843352562619367E-5
34	0.11200307459420454	0.0	7.843352562619367E-5
35	0.11423843007455106	0.0	7.843352562619367E-5
36	0.1163169185036452	0.0	7.843352562619367E-5
37	0.1187483577980572	0.0	7.843352562619367E-5
38	0.12168961500903946	0.0	7.843352562619367E-5
39	0.12427792135470385	0.0	7.843352562619367E-5
40	0.12674857741192896	0.0	7.843352562619367E-5
41	0.12925845023196716	0.0	7.843352562619367E-5
42	0.13212127391732323	0.0	7.843352562619367E-5
43	0.1360037334358198	0.0	7.843352562619367E-5
44	0.13933715827493304	0.0	7.843352562619367E-5
45	0.14439612067782254	0.0	7.843352562619367E-5
46	0.1484746640103846	0.0	7.843352562619367E-5
47	0.15180808884949784	0.0	7.843352562619367E-5
48	0.1586318055789767	0.0	7.843352562619367E-5
49	0.16231818128340778	0.0	7.843352562619367E-5
50	0.16718105987223178	0.0	7.843352562619367E-5
51	0.17263218990325224	0.0	7.843352562619367E-5
52	0.17663229971018812	0.0	7.843352562619367E-5
53	0.18169126211307762	0.0	7.843352562619367E-5
54	0.18722082566972426	0.0	7.843352562619367E-5
55	0.19282882275199711	0.0	7.843352562619367E-5
56	0.19777013486644732	0.0	7.843352562619367E-5
57	0.20361343252559874	0.0	7.843352562619367E-5
58	0.21188816947916217	0.0	7.843352562619367E-5
59	0.22094724168898755	0.0	7.843352562619367E-5
60	0.22922197864255098	0.0	7.843352562619367E-5
61	0.23843791790362873	0.0	7.843352562619367E-5
62	0.2500460796963054	0.0	7.843352562619367E-5
63	0.25785021549611165	0.0	7.843352562619367E-5
64	0.2670269379943763	0.0	7.843352562619367E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATGCC	42860	0.0	67.901306	45
TATGCCG	42915	0.0	67.82244	46
CGTATGC	42985	0.0	67.72828	44
ATGCCGT	42925	0.0	67.7251	47
GCACACG	43550	0.0	67.71757	11
CTCGTAT	42695	0.0	67.663666	42
TGCCGTC	42975	0.0	67.581154	48
ACACGTC	43610	0.0	67.57625	13
AGCACAC	43695	0.0	67.52489	10
CACACGT	43665	0.0	67.50716	12
ACGTCTG	43620	0.0	67.48854	15
TCGTATG	43145	0.0	67.469	43
CACGTCT	43695	0.0	67.39673	14
TCTCGTA	42010	0.0	67.3423	41
CAGTCAC	43200	0.0	67.28588	27
GCCGTCT	43135	0.0	67.273674	49
GTCACAC	42180	0.0	67.25344	29
GAGCACA	43970	0.0	67.12645	9
TCGGAAG	43940	0.0	67.12449	3
CGTCTGA	42815	0.0	67.089806	16
>>END_MODULE