FastQCFastQC Report
Mon 15 Sep 2014
TGA3_3+7_ACTTGA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameTGA3_3+7_ACTTGA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2549930
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC35829414.051130815355716TruSeq Adapter, Index 8 (100% over 50bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC119710.4694638676355821No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG87510.34318589137741035No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG62850.2464773542803136No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTG53350.2092214296078716No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA38380.15051393567666563No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAACCTCGTATGC36330.14247449929998077TruSeq Adapter, Index 8 (98% over 50bp)
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA35510.13925872474930684No Hit
GCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTC33650.13196440686607083No Hit
GGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCT32710.12827803116163972No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT31440.12329750228437643No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC30240.1185914907468048No Hit
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTA27600.10823826536414725No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGTATCTCGTATGC26570.10419893879439829TruSeq Adapter, Index 8 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC428600.067.90130645
TATGCCG429150.067.8224446
CGTATGC429850.067.7282844
ATGCCGT429250.067.725147
GCACACG435500.067.7175711
CTCGTAT426950.067.66366642
TGCCGTC429750.067.58115448
ACACGTC436100.067.5762513
AGCACAC436950.067.5248910
CACACGT436650.067.5071612
ACGTCTG436200.067.4885415
TCGTATG431450.067.46943
CACGTCT436950.067.3967314
TCTCGTA420100.067.342341
CAGTCAC432000.067.2858827
GCCGTCT431350.067.27367449
GTCACAC421800.067.2534429
GAGCACA439700.067.126459
TCGGAAG439400.067.124493
CGTCTGA428150.067.08980616
Produced by FastQC (version 0.11.2)